##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938866_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4151080 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360271784692177 33.0 31.0 34.0 28.0 34.0 2 31.429110496545476 33.0 31.0 34.0 28.0 34.0 3 31.43923051350492 33.0 31.0 34.0 28.0 34.0 4 34.98960728292396 37.0 35.0 37.0 32.0 37.0 5 35.01113300635016 37.0 35.0 37.0 32.0 37.0 6 35.064859265540534 37.0 35.0 37.0 32.0 37.0 7 34.95964760977866 37.0 35.0 37.0 32.0 37.0 8 34.91971197856943 37.0 35.0 37.0 32.0 37.0 9 36.46283762298005 39.0 37.0 39.0 32.0 39.0 10-11 36.33032138624165 39.0 37.0 39.0 32.0 39.0 12-13 36.19639696175453 39.0 37.0 39.0 32.0 39.0 14-15 37.31663591161818 40.0 37.0 41.0 31.5 41.0 16-17 37.18499101438662 40.0 37.0 41.0 31.0 41.0 18-19 37.10325879048344 40.0 37.0 41.0 31.0 41.0 20-21 36.985182892163 40.0 36.5 41.0 30.5 41.0 22-23 37.008003700241865 40.0 37.0 41.0 30.5 41.0 24-25 36.931589128612316 40.0 36.0 41.0 30.0 41.0 26-27 36.7943253322027 40.0 36.0 41.0 30.0 41.0 28-29 36.605478453799975 39.5 36.0 41.0 30.0 41.0 30-31 36.36240833710745 39.0 36.0 41.0 29.5 41.0 32-33 36.165428635439454 39.0 35.0 41.0 29.0 41.0 34-35 35.85967097719148 39.0 35.0 41.0 27.5 41.0 36-37 35.63368159611474 39.0 35.0 41.0 26.5 41.0 38-39 35.45495461422088 39.0 35.0 40.0 26.0 41.0 40-41 35.32196765660984 38.5 35.0 40.0 25.5 41.0 42-43 35.29683961764168 39.0 35.0 41.0 25.0 41.0 44-45 35.117943041329 39.0 35.0 41.0 24.5 41.0 46-47 34.802642565308304 38.5 34.0 41.0 23.5 41.0 48-49 34.50704370910702 38.0 34.0 40.0 23.0 41.0 50-51 33.37329478111721 37.5 32.5 39.5 20.5 40.5 52-53 33.440544749800054 37.5 33.0 39.5 20.0 40.5 54-55 33.908426361332474 38.0 33.0 40.0 20.5 41.0 56-57 33.78442321034526 38.0 33.0 40.0 20.0 41.0 58-59 33.55915075112982 38.0 33.0 40.0 18.5 41.0 60-61 33.23121235919327 37.0 33.0 40.0 17.5 41.0 62-63 32.82019594900604 36.5 32.0 40.0 14.0 41.0 64-65 32.40880662381838 36.0 31.5 39.0 12.0 41.0 66-67 32.01269958661361 35.5 31.0 39.0 8.0 41.0 68-69 31.584501503223258 35.0 31.0 39.0 7.5 40.0 70-71 31.146059941027396 35.0 30.5 38.0 4.5 40.0 72-73 30.721113661986763 35.0 30.0 37.0 2.0 39.5 74-75 30.25253283482853 35.0 30.0 37.0 2.0 39.0 76-77 29.790176291471134 34.0 29.0 36.0 2.0 39.0 78-79 29.353167368492056 34.0 29.0 36.0 2.0 37.5 80-81 28.88636077839984 34.0 29.0 35.0 2.0 37.0 82-83 28.468230195515382 34.0 28.0 35.0 2.0 37.0 84-85 28.11592910760573 34.0 27.0 35.0 2.0 36.0 86-87 27.68453474758376 34.0 26.5 35.0 2.0 36.0 88-89 27.22436149146728 33.5 26.0 35.0 2.0 35.5 90-91 26.765040182314003 33.0 25.0 35.0 2.0 35.0 92-93 26.277305183229423 33.0 24.5 35.0 2.0 35.0 94-95 25.7337549505189 33.0 21.5 35.0 2.0 35.0 96-97 25.094823756709097 32.0 18.5 35.0 2.0 35.0 98-99 24.27821807818688 32.0 2.0 34.5 2.0 35.0 100-101 21.750576837834974 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.043394537264418886 1101 2 0.20009376018052905 1101 3 0.2967637591306236 1101 4 0.10111139416493131 1101 5 0.15890087135656472 1101 6 0.31054699522310614 1101 7 0.3663491728292101 1101 8 0.4257833855696873 1101 9 0.4981347511212988 1101 10-11 0.39956619092211554 1101 12-13 0.4042497670884302 1101 14-15 0.4155214387778372 1101 16-17 0.5402114553919404 1101 18-19 0.5162744141225062 1101 20-21 0.6148222599769113 1101 22-23 0.5794339326410523 1101 24-25 0.5164548441377264 1101 26-27 0.5461080417237838 1101 28-29 0.5971183764495294 1101 30-31 0.5764808589270061 1101 32-33 0.43724242945064873 1101 34-35 0.5085539387879408 1101 36-37 0.5681198328016634 1101 38-39 0.46080798878778495 1101 40-41 0.6275547689826269 1101 42-43 0.7505585991739139 1101 44-45 0.7741048426512762 1101 46-47 0.7038752758526741 1101 48-49 0.6114943174828227 1101 50-51 0.644206297949971 1101 52-53 0.7146860065331921 1101 54-55 0.6953178113141476 1101 56-57 0.7042874937167198 1101 58-59 0.6687707107825176 1101 60-61 0.6062254567315435 1101 62-63 0.5495525036917286 1101 64-65 0.5455850113161844 1101 66-67 0.4789784514035702 1101 68-69 0.5816557654735242 1101 70-71 0.36506186743332947 1101 72-73 0.3422050005349142 1101 74-75 0.3757626466385844 1101 76-77 0.3812720494455135 1101 78-79 0.349419524808372 1101 80-81 0.3000117040479964 1101 82-83 0.3159040672059632 1101 84-85 0.22487602269506723 1101 86-87 0.2003706989309748 1101 88-89 0.0865053836683991 1101 90-91 0.12545084669325846 1101 92-93 0.20072941383468645 1101 94-95 0.17940626522843672 1101 96-97 0.02075124766570724 1101 98-99 0.06743411824121281 1101 100-101 0.34804851699418293 1102 1 0.15439600245930762 1102 2 0.23762893072962754 1102 3 0.23597855006921264 1102 4 0.3686404833377992 1102 5 0.35135202058930304 1102 6 0.3313431834597793 1102 7 0.2954511716239878 1102 8 0.3412491473754642 1102 9 0.39181636010469845 1102 10-11 0.450915480441914 1102 12-13 0.5251980012442132 1102 14-15 0.5895402152859859 1102 16-17 0.6573956147078732 1102 18-19 0.7329856721752606 1102 20-21 0.6642839280792927 1102 22-23 0.5121439421810479 1102 24-25 0.5983057700566192 1102 26-27 0.5924920061409367 1102 28-29 0.6766477985898405 1102 30-31 0.7278722414257786 1102 32-33 0.8070649412271251 1102 34-35 0.7771585519297517 1102 36-37 0.8160296907332523 1102 38-39 0.8705260326063851 1102 40-41 0.8227516331017455 1102 42-43 0.9277273834996649 1102 44-45 0.9157585970433502 1102 46-47 1.0231142541354146 1102 48-49 1.0035943311377125 1102 50-51 1.1082627393846565 1102 52-53 0.9746398562331677 1102 54-55 0.899500593858086 1102 56-57 0.9271735592136636 1102 58-59 0.9064105035690062 1102 60-61 0.7568830904475519 1102 62-63 0.7130106169176926 1102 64-65 0.7434795218897392 1102 66-67 0.7083154731687173 1102 68-69 0.7805625339001914 1102 70-71 0.6663390830886904 1102 72-73 0.5961401644522972 1102 74-75 0.5488296681941947 1102 76-77 0.5606063885891999 1102 78-79 0.5468545357127077 1102 80-81 0.4899843643357791 1102 82-83 0.5613662920962241 1102 84-85 0.4436736233952665 1102 86-87 0.3596081735640837 1102 88-89 0.4018806386613569 1102 90-91 0.30421653916535973 1102 92-93 0.36778805409791104 1102 94-95 0.26904456495761764 1102 96-97 0.3351850521963229 1102 98-99 -0.04852467024548446 1102 100-101 0.201613536715481 1103 1 0.25402256176200666 1103 2 0.36014118133155293 1103 3 0.48941710830555607 1103 4 0.4539459244280124 1103 5 0.40261925020224965 1103 6 0.3521506542224415 1103 7 0.3840648502193531 1103 8 0.45890483348905065 1103 9 0.47748055850446036 1103 10-11 0.47812441541931605 1103 12-13 0.4951456327605186 1103 14-15 0.5354900814870192 1103 16-17 0.6354665245941433 1103 18-19 0.7310502634462921 1103 20-21 0.6158869268893312 1103 22-23 0.5892848078732627 1103 24-25 0.6367651994370576 1103 26-27 0.7216095436008558 1103 28-29 0.7776522000939323 1103 30-31 0.7357560366872278 1103 32-33 0.7011383191914646 1103 34-35 0.8334969886089425 1103 36-37 0.8205476649106558 1103 38-39 0.8631126212577342 1103 40-41 1.0096541078757184 1103 42-43 1.0293141354130668 1103 44-45 0.9999223934060595 1103 46-47 0.9564293824094179 1103 48-49 0.8304319837398637 1103 50-51 0.8817711150904017 1103 52-53 0.7753888053116071 1103 54-55 0.8253145050310664 1103 56-57 0.8263050300302623 1103 58-59 0.7659680917364611 1103 60-61 0.8247282160556679 1103 62-63 0.831568747894643 1103 64-65 0.650687903974152 1103 66-67 0.6422017353752807 1103 68-69 0.6849663152804162 1103 70-71 0.6157174523511948 1103 72-73 0.6769183126255811 1103 74-75 0.5418846863722528 1103 76-77 0.5471261662401083 1103 78-79 0.5877772280160904 1103 80-81 0.5767237016867277 1103 82-83 0.5870841154516704 1103 84-85 0.6029371416006839 1103 86-87 0.6078936387889833 1103 88-89 0.6167410314805011 1103 90-91 0.5795238281417951 1103 92-93 0.5156445853900102 1103 94-95 0.6042026340060218 1103 96-97 0.5676144130350238 1103 98-99 0.6908769205228324 1103 100-101 1.095915181311561 1104 1 0.22956807431789272 1104 2 0.289149703923961 1104 3 0.2333950202213657 1104 4 0.41568852682434 1104 5 0.37160956865184147 1104 6 0.3559183389597038 1104 7 0.39082746826466064 1104 8 0.4105455123060153 1104 9 0.41727681734111854 1104 10-11 0.3990218952212814 1104 12-13 0.44158833757770566 1104 14-15 0.5232901541276789 1104 16-17 0.6564439688898958 1104 18-19 0.6404023091740427 1104 20-21 0.6685037385911343 1104 22-23 0.5607286092915942 1104 24-25 0.5378222891128743 1104 26-27 0.5672775349466761 1104 28-29 0.6297766914429559 1104 30-31 0.6894078932808938 1104 32-33 0.5609623319036956 1104 34-35 0.64261202189995 1104 36-37 0.733817423943691 1104 38-39 0.7437791878677658 1104 40-41 0.8188326538243231 1104 42-43 0.8004609467957593 1104 44-45 0.7965160176035013 1104 46-47 0.9001542641822837 1104 48-49 0.9126659626790286 1104 50-51 0.9862414871826743 1104 52-53 0.9568931244542256 1104 54-55 0.8834217646885136 1104 56-57 0.8153720011818208 1104 58-59 0.8547694429746002 1104 60-61 0.7932763984547719 1104 62-63 0.7596107673953156 1104 64-65 0.8047897391488519 1104 66-67 0.8404510130562315 1104 68-69 0.8198790399243698 1104 70-71 0.8552849415833599 1104 72-73 0.8308677794406272 1104 74-75 0.6997101449065752 1104 76-77 0.6790593212681628 1104 78-79 0.5570808248340171 1104 80-81 0.5906856612977514 1104 82-83 0.6934573583386197 1104 84-85 0.7239150256645139 1104 86-87 0.79448624362659 1104 88-89 0.9672358720425862 1104 90-91 0.9763343933102924 1104 92-93 0.9788951694076466 1104 94-95 1.0248520113226292 1104 96-97 0.9221782076516085 1104 98-99 1.0725028862788832 1104 100-101 1.3183297425510077 1105 1 0.10284056634174377 1105 2 0.12410880235387367 1105 3 0.22980336583990635 1105 4 0.2373977018836797 1105 5 0.20328780890245923 1105 6 0.14449431310778493 1105 7 0.22021648001172878 1105 8 0.21329677726740215 1105 9 0.2633924352836132 1105 10-11 0.28524865379790754 1105 12-13 0.2742716464503232 1105 14-15 0.34393924316459135 1105 16-17 0.44558377539978977 1105 18-19 0.2857081455822481 1105 20-21 0.31531056816488956 1105 22-23 0.2641175165988443 1105 24-25 0.31231797756979063 1105 26-27 0.31573381141728163 1105 28-29 0.29833355047269094 1105 30-31 0.4973489501722881 1105 32-33 0.3551746549676267 1105 34-35 0.3413962525416636 1105 36-37 0.4794893701598184 1105 38-39 0.43981134172604186 1105 40-41 0.5498089751689506 1105 42-43 0.49723882503955963 1105 44-45 0.5695648526498118 1105 46-47 0.6992617645624151 1105 48-49 0.8413160737340917 1105 50-51 0.8656718140902555 1105 52-53 0.8038953288802375 1105 54-55 0.8482194597282202 1105 56-57 0.745572856930032 1105 58-59 0.8087575078738496 1105 60-61 0.6986350054909849 1105 62-63 0.638838742169149 1105 64-65 0.6182687938105857 1105 66-67 0.589807424662304 1105 68-69 0.671152360884367 1105 70-71 0.6218116640229354 1105 72-73 0.7265282943245062 1105 74-75 0.5294690293665241 1105 76-77 0.5144340945339394 1105 78-79 0.5677478375855465 1105 80-81 0.691126809793392 1105 82-83 0.6441113578019397 1105 84-85 0.6380551338650342 1105 86-87 0.7012061884929004 1105 88-89 0.6417122303580243 1105 90-91 0.7305373277618052 1105 92-93 0.8013560478122805 1105 94-95 0.7103624027547006 1105 96-97 0.6507139066490524 1105 98-99 0.7776157635539853 1105 100-101 1.121483791930416 1106 1 0.17651516949509727 1106 2 0.18516704222621883 1106 3 0.302676324044171 1106 4 0.2224477812663963 1106 5 0.3277782200594146 1106 6 0.29927411643141255 1106 7 0.27516773929200866 1106 8 0.2944516686081329 1106 9 0.21594084803089686 1106 10-11 0.35577501608830175 1106 12-13 0.4416719896835204 1106 14-15 0.4773892989868429 1106 16-17 0.542890465769716 1106 18-19 0.5083776270109723 1106 20-21 0.49523043579788606 1106 22-23 0.49539029060307627 1106 24-25 0.3572949659528746 1106 26-27 0.3413934376090282 1106 28-29 0.24155014346827386 1106 30-31 0.22562060237692805 1106 32-33 0.3405266564026732 1106 34-35 0.4245883747776631 1106 36-37 0.6750953159653861 1106 38-39 0.5784289579956194 1106 40-41 0.6543568417417234 1106 42-43 0.7047883756503879 1106 44-45 0.7478119270018411 1106 46-47 0.7663759819666396 1106 48-49 0.7869394489318822 1106 50-51 0.7178422955002404 1106 52-53 0.4994030435950876 1106 54-55 0.4154909700345897 1106 56-57 0.6403733292103411 1106 58-59 0.711109779718818 1106 60-61 0.8763890234879739 1106 62-63 0.7377012266741261 1106 64-65 0.7818756338430362 1106 66-67 0.8420701399527744 1106 68-69 0.8489474758353559 1106 70-71 0.8081566321225964 1106 72-73 0.8178911335751771 1106 74-75 0.6081665102704967 1106 76-77 0.6817719222051757 1106 78-79 0.7700461345384824 1106 80-81 0.766236245493392 1106 82-83 0.7424088729756839 1106 84-85 0.889656763821101 1106 86-87 0.8584959313892853 1106 88-89 0.9468964169997776 1106 90-91 1.0985267630334299 1106 92-93 1.2517486508960225 1106 94-95 1.368377995501291 1106 96-97 1.317012423142721 1106 98-99 1.414178639216292 1106 100-101 1.8515028544914927 1107 1 0.04773770141877165 1107 2 0.1207704558443865 1107 3 0.10026043756969827 1107 4 0.23425775992792097 1107 5 0.09402653429299335 1107 6 0.017786097306114357 1107 7 0.029387392471200258 1107 8 0.06924655974425775 1107 9 0.11503016440639868 1107 10-11 0.07965254104033193 1107 12-13 0.08608425386110241 1107 14-15 0.23498505656993274 1107 16-17 0.2616148364057196 1107 18-19 0.22348887078725 1107 20-21 0.23828550842534213 1107 22-23 0.2283171575851881 1107 24-25 0.1713222636037557 1107 26-27 0.2726480511970806 1107 28-29 0.06604286727687736 1107 30-31 0.1135147355736521 1107 32-33 0.1554670579209727 1107 34-35 0.21825091493016657 1107 36-37 0.16865566808383647 1107 38-39 0.10242714550259535 1107 40-41 0.18848837118733996 1107 42-43 0.18050788025356468 1107 44-45 0.33981197540669683 1107 46-47 0.42911220103627556 1107 48-49 0.46664087163134127 1107 50-51 0.4707092211328998 1107 52-53 0.3604750498554523 1107 54-55 0.3047363786320929 1107 56-57 0.5039642167268639 1107 58-59 0.5440847288600992 1107 60-61 0.5155048280775603 1107 62-63 0.4692137574817963 1107 64-65 0.5024481019664151 1107 66-67 0.3578036819648318 1107 68-69 0.5244965415543668 1107 70-71 0.6068281876799446 1107 72-73 0.6165279183093588 1107 74-75 0.6523558969674994 1107 76-77 0.5438932382257065 1107 78-79 0.4701217267920903 1107 80-81 0.3609962851315913 1107 82-83 0.32679177153926986 1107 84-85 0.486263556367291 1107 86-87 0.4246041673414567 1107 88-89 0.4214280932734624 1107 90-91 0.6339689615820951 1107 92-93 0.7388992585786802 1107 94-95 0.7249860250605238 1107 96-97 0.6906706875327444 1107 98-99 0.9862671340537794 1107 100-101 1.403582071731151 1108 1 0.32773126891823523 1108 2 0.24486030005078163 1108 3 0.21064101500216026 1108 4 0.2478215408942006 1108 5 0.3114761185678887 1108 6 0.18296996413260302 1108 7 0.28407568441240727 1108 8 0.29196216434684175 1108 9 0.2924945542791235 1108 10-11 0.42114162718785764 1108 12-13 0.41622689125942003 1108 14-15 0.51071591348488 1108 16-17 0.6371798170993728 1108 18-19 0.6597603984719385 1108 20-21 0.653299307765991 1108 22-23 0.5714715787444362 1108 24-25 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2316 58-59 -1.9448474643768314 2316 60-61 -1.7921378475125636 2316 62-63 -2.2005897158121606 2316 64-65 -2.3727696385011754 2316 66-67 -2.141648607131266 2316 68-69 -2.265113868524999 2316 70-71 -2.2414340289585333 2316 72-73 -2.124886649659363 2316 74-75 -2.243122428493187 2316 76-77 -2.271277738684418 2316 78-79 -2.15548458184146 2316 80-81 -2.237570827450952 2316 82-83 -2.150407585051731 2316 84-85 -2.1810961791464685 2316 86-87 -2.211951542103133 2316 88-89 -2.2832353719610907 2316 90-91 -2.7358973140509555 2316 92-93 -2.537907720499856 2316 94-95 -2.5233340086633085 2316 96-97 -2.8405768235459803 2316 98-99 -3.1044715491238115 2316 100-101 -3.0482530154826257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24540.0 3 6825.0 4 6694.0 5 7444.0 6 8397.0 7 9982.0 8 12262.0 9 14224.0 10 16388.0 11 19750.0 12 23136.0 13 26654.0 14 28572.0 15 31078.0 16 35414.0 17 41471.0 18 38495.0 19 32192.0 20 32780.0 21 34661.0 22 37929.0 23 41877.0 24 47473.0 25 53740.0 26 60521.0 27 70250.0 28 81760.0 29 94686.0 30 113113.0 31 134437.0 32 163644.0 33 205175.0 34 269583.0 35 372429.0 36 534800.0 37 739339.0 38 594527.0 39 84791.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2620119362056 18.259650397591695 13.989289977906472 23.48904768829623 2 15.564417445869259 19.78486455520842 40.08967475827607 24.561043240646253 3 19.579659154654884 23.857146581263837 29.37432836512606 27.188865898955218 4 13.035759702444064 16.078232462920127 36.34838599456295 34.53762184007285 5 14.19830970433711 36.849739771547604 33.62929646194106 15.322654062174232 6 32.839297455588905 35.16263066984256 17.146461257423574 14.851610617144964 7 29.274989718401322 32.307016868100675 20.21742653094393 18.200566882554078 8 26.755512557212164 34.46246650347475 19.452883647513193 19.329137291799896 9 26.881382397887666 16.74926015694854 20.03130188732961 36.33805555783418 10-11 25.537259940206614 26.03355058496441 26.769108915862578 21.660080558966392 12-13 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28.283240329470832 22.82189130442407 38-39 22.600163089373172 26.10955910732267 28.611870991082323 22.678406812221834 40-41 22.867288854347528 25.903273042408316 28.70143754241708 22.528000560827074 42-43 22.663919778417572 26.16649247729211 28.263753504543516 22.90583423974681 44-45 22.702085617967665 26.279216868161893 28.173430393487344 22.845267120383102 46-47 22.90862655043189 26.441912238631982 27.739867246631285 22.909593964304847 48-49 23.035506899126716 26.572989037789473 27.519739695577744 22.87176436750607 50-51 22.79610526085365 26.6765249797212 27.58960617583362 22.937763583591533 52-53 23.20272464853427 26.573958374150813 27.44589234634123 22.777424630973684 54-55 22.65664738094852 26.504519417495686 27.62295159285552 23.21588160870027 56-57 22.812451561716752 26.85232226883045 27.426061265405643 22.90916490404716 58-59 23.089679190847868 26.388173707197282 27.423789030184615 23.098358071770235 60-61 22.817951044188607 26.375531857818086 27.991707778959817 22.81480931903349 62-63 22.75649350492312 26.467057382167454 27.915565693457133 22.86088341945229 64-65 22.57586334238154 26.486279939244252 27.713878010223773 23.223978708150437 66-67 22.547316362482132 26.766033588321093 27.399833696270942 23.286816352925836 68-69 22.596201304168577 26.505416615858675 27.71404197417599 23.184340105796757 70-71 22.58410065610149 26.525354918535314 27.54877453364244 23.341769891720755 72-73 22.685492346997098 26.41136053344642 27.66418693171901 23.238960187837474 74-75 22.53971663786695 26.798857494252758 27.361522463659256 23.29990340422104 76-77 22.728367745187484 26.559212060289838 27.42223349880564 23.29018669571704 78-79 22.762828399482338 26.634380458816224 27.401153714461472 23.201637427239966 80-81 22.745259736569405 26.86792557777345 27.29958851816965 23.087226167487493 82-83 22.7576738533858 26.760670002627794 27.256855825796706 23.224800318189693 84-85 22.72351673611503 26.640467478585933 27.151326250756874 23.484689534542163 86-87 22.92603057113645 26.495916048788537 27.31135192721745 23.266701452857557 88-89 22.787528105323855 26.534291881286208 27.049342499893577 23.628837513496357 90-91 22.802931609881544 26.581824647731437 27.26475814460399 23.350485597783027 92-93 23.01973404490162 26.286287291260567 27.31684364145716 23.377135022380653 94-95 22.80051910749125 26.174693606577687 27.286978666656342 23.73780861927472 96-97 22.960859057064216 25.903678951289784 27.39322846698931 23.74223352465669 98-99 22.782493072218763 26.033699476309575 27.10681000774673 24.07699744372493 100-101 23.139167890721954 25.425706394587372 27.590440009278066 23.8446857054126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4426.0 1 2989.5 2 1595.0 3 1666.5 4 1543.5 5 1482.0 6 2166.5 7 3894.0 8 5977.0 9 6719.0 10 5684.0 11 4626.5 12 4496.0 13 4678.5 14 4999.5 15 5336.5 16 5583.0 17 5885.0 18 6146.0 19 6593.0 20 7303.0 21 8470.0 22 10042.5 23 11823.0 24 14240.0 25 17414.5 26 21407.5 27 26456.0 28 31193.5 29 36164.0 30 42237.0 31 49159.5 32 57538.0 33 63444.0 34 69331.0 35 75455.0 36 81636.5 37 89695.0 38 96370.5 39 99895.0 40 103826.5 41 113040.5 42 122452.5 43 129445.0 44 135363.5 45 141953.5 46 145280.0 47 151045.0 48 167114.0 49 170477.0 50 162367.5 51 157590.5 52 151387.5 53 151268.5 54 152283.0 55 143555.5 56 131512.0 57 119641.0 58 107859.0 59 95607.0 60 82056.0 61 68305.0 62 57312.0 63 46162.5 64 35426.0 65 28074.5 66 21329.0 67 16990.5 68 12489.5 69 8239.0 70 6281.0 71 4931.0 72 3970.0 73 3277.0 74 2570.5 75 2055.5 76 1552.5 77 1009.5 78 645.0 79 454.5 80 323.0 81 238.5 82 181.5 83 147.5 84 114.0 85 77.0 86 55.5 87 40.0 88 30.0 89 23.5 90 23.5 91 23.0 92 17.0 93 14.0 94 10.0 95 3.0 96 3.0 97 4.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27477186659857195 2 0.16846218333542115 3 0.19763531418329686 4 0.16665542461239002 5 0.23875714271948506 6 0.19744259325284022 7 0.24540601482023952 8 0.24938088401090802 9 0.15133411064108618 10-11 0.2083433708817946 12-13 0.21496815286624205 14-15 0.1755687676460102 16-17 0.2901413608024899 18-19 0.23505930986634804 20-21 0.20082725459398518 22-23 0.24857386511462076 24-25 0.23681788835676498 26-27 0.34665677365890324 28-29 0.3196156181042042 30-31 0.2943450860980757 32-33 0.19511789702920684 34-35 0.3643268739701475 36-37 0.4035937635506904 38-39 0.3836110120739663 40-41 0.3452715919712461 42-43 0.3790338899756208 44-45 0.38829653969569367 46-47 0.3937529510392476 48-49 0.41316958478275534 50-51 0.40559324320417817 52-53 0.40524393651772556 54-55 0.39958275918556135 56-57 0.377323491717818 58-59 0.35176387831600453 60-61 0.3186158782774603 62-63 0.3878990527766268 64-65 0.4210831879896316 66-67 0.4258891661928944 68-69 0.43053855864016116 70-71 0.43068309933800364 72-73 0.42173362112992285 74-75 0.4181803289746283 76-77 0.4011486167455216 78-79 0.3750349306686453 80-81 0.3860441138209815 82-83 0.3960294670302668 84-85 0.39684853098470757 86-87 0.39260867051466125 88-89 0.40066681441937996 90-91 0.421938387118533 92-93 0.42127590892008826 94-95 0.42957495398787787 96-97 0.43002062113955886 98-99 0.42692504119409885 100-101 0.39987184058124636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4151080.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.6733621681017 #Duplication Level Percentage of deduplicated Percentage of total 1 71.01163152292168 32.43339964694189 2 14.550980018698533 13.291843605896588 3 5.8223086175850804 7.9777323043626875 4 2.830427392312898 5.171005415184906 5 1.6163352334314787 3.691173225078956 6 1.0173489840999708 2.7879449161288985 7 0.6266484731591452 2.0034799866679913 8 0.43778587762514853 1.5996122352682927 9 0.3187870909013614 1.3104070431528094 >10 1.552841998213129 12.632836330070143 >50 0.1164474384724476 3.6868432887842415 >100 0.08744020950097887 7.764567984796031 >500 0.007044615610746974 2.169063257816418 >1k 0.0038136263687403695 2.626772524888409 >5k 5.2967032899171475E-5 0.18545452865413342 >10k+ 1.0593406579834295E-4 0.6678637063076783 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15305 0.3686992300798828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12302 0.296356610809717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7666 0.18467483161008702 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4325 0.10418975302812761 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.409011630708153E-5 2 0.0 0.0 0.0 0.0 2.409011630708153E-5 3 0.0 0.0 0.0 0.0 2.409011630708153E-5 4 0.0 0.0 0.0 0.0 2.409011630708153E-5 5 0.0 0.0 0.0 0.0 2.409011630708153E-5 6 0.0 0.0 0.0 0.0 2.409011630708153E-5 7 0.0 0.0 0.0 0.0 2.409011630708153E-5 8 0.0 0.0 0.0 0.0 2.409011630708153E-5 9 0.0 0.0 0.0 4.818023261416306E-5 2.409011630708153E-5 10-11 0.0 0.0 0.0 4.818023261416306E-5 2.409011630708153E-5 12-13 0.0 0.0 0.0 7.22703489212446E-5 2.409011630708153E-5 14-15 0.0 0.0 0.0 7.22703489212446E-5 2.409011630708153E-5 16-17 0.0 0.0 0.0 8.431540707478536E-5 2.409011630708153E-5 18-19 0.0 0.0 0.0 1.8067587230311148E-4 2.409011630708153E-5 20-21 0.0 0.0 0.0 2.890813956849784E-4 2.409011630708153E-5 22-23 0.0 0.0 0.0 4.6975726798808984E-4 2.409011630708153E-5 24-25 0.0 0.0 0.0 7.588386636730683E-4 2.409011630708153E-5 26-27 0.0 0.0 0.0 0.001288821222428862 2.409011630708153E-5 28-29 0.0 0.0 0.0 0.0033123909922237104 2.409011630708153E-5 30-31 0.0 0.0 0.0 0.009840812511442806 2.409011630708153E-5 32-33 0.0 2.409011630708153E-5 0.0 0.02215086194436147 2.409011630708153E-5 34-35 0.0 2.409011630708153E-5 0.0 0.04233837940969579 2.409011630708153E-5 36-37 0.0 2.409011630708153E-5 0.0 0.07696792160112549 2.409011630708153E-5 38-39 0.0 2.409011630708153E-5 0.0 0.12728012950846526 2.409011630708153E-5 40-41 1.2045058153540766E-5 2.409011630708153E-5 0.0 0.18132630544340267 2.409011630708153E-5 42-43 2.409011630708153E-5 2.409011630708153E-5 0.0 0.2380103491139655 2.409011630708153E-5 44-45 2.409011630708153E-5 2.409011630708153E-5 0.0 0.3115936093739461 2.409011630708153E-5 46-47 2.409011630708153E-5 2.409011630708153E-5 0.0 0.40462963855189493 2.409011630708153E-5 48-49 2.409011630708153E-5 2.409011630708153E-5 0.0 0.5035797912832323 2.409011630708153E-5 50-51 2.409011630708153E-5 2.409011630708153E-5 0.0 0.6099256097208438 2.409011630708153E-5 52-53 2.409011630708153E-5 2.409011630708153E-5 0.0 0.715934166530156 2.409011630708153E-5 54-55 2.409011630708153E-5 2.409011630708153E-5 0.0 0.8357824951578867 2.409011630708153E-5 56-57 2.409011630708153E-5 2.409011630708153E-5 0.0 0.9791427773013288 2.409011630708153E-5 58-59 2.409011630708153E-5 2.409011630708153E-5 0.0 1.1376075623693112 2.409011630708153E-5 60-61 2.409011630708153E-5 2.409011630708153E-5 0.0 1.3111768503618335 2.409011630708153E-5 62-63 2.409011630708153E-5 2.409011630708153E-5 0.0 1.4875405918459774 2.409011630708153E-5 64-65 2.409011630708153E-5 2.409011630708153E-5 0.0 1.6732272083409618 2.409011630708153E-5 66-67 2.409011630708153E-5 2.409011630708153E-5 0.0 1.8923509062701755 2.409011630708153E-5 68-69 2.409011630708153E-5 2.409011630708153E-5 0.0 2.1404550141168084 2.409011630708153E-5 70-71 2.409011630708153E-5 2.409011630708153E-5 0.0 2.411276101641019 2.409011630708153E-5 72-73 2.409011630708153E-5 2.409011630708153E-5 0.0 2.6842171194002526 2.409011630708153E-5 74-75 2.409011630708153E-5 2.409011630708153E-5 0.0 2.964939244726674 2.409011630708153E-5 76-77 2.409011630708153E-5 2.409011630708153E-5 0.0 3.2729675168871717 2.409011630708153E-5 78-79 2.409011630708153E-5 2.409011630708153E-5 0.0 3.623611204794897 2.409011630708153E-5 80-81 2.409011630708153E-5 2.409011630708153E-5 1.2045058153540766E-5 4.0018742110486905 2.409011630708153E-5 82-83 2.409011630708153E-5 2.409011630708153E-5 2.409011630708153E-5 4.382510093758732 2.409011630708153E-5 84-85 2.409011630708153E-5 2.409011630708153E-5 2.409011630708153E-5 4.772480896537768 2.409011630708153E-5 86-87 2.409011630708153E-5 2.409011630708153E-5 2.409011630708153E-5 5.179061834510537 2.409011630708153E-5 88-89 2.409011630708153E-5 2.409011630708153E-5 2.409011630708153E-5 5.618814862638157 2.409011630708153E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14145 0.0 30.740871 1 GTATCAA 20775 0.0 30.37771 1 ATCAACG 24835 0.0 25.043266 3 TATCAAC 25015 0.0 24.938923 2 TCAACGC 24940 0.0 24.861746 4 GTACATG 17000 0.0 24.627766 1 CAACGCA 25255 0.0 24.532866 5 AACGCAG 25890 0.0 23.874737 6 ACATGGG 16685 0.0 23.40055 3 TACATGG 17415 0.0 23.318615 2 GAGTACT 14150 0.0 22.272892 12-13 ACGCAGA 28495 0.0 21.54255 7 CGCAGAG 28770 0.0 21.320145 8 CATGGGG 9380 0.0 20.989288 4 GTACTTT 15190 0.0 20.716831 14-15 GCAGAGT 30130 0.0 19.899765 9 AGTACTT 14820 0.0 19.872776 12-13 AGAGTAC 23850 0.0 19.193197 10-11 TACCTGG 3100 0.0 19.129375 2 ACTTTTT 17800 0.0 18.01224 16-17 >>END_MODULE