FastQCFastQC Report
Thu 26 May 2016
SRR938866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4151080
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149480.36009905855825475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132540.3192904015340586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84630.203874654306831No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47770.11507848559892846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153650.029.8407021
GTATCAA214650.026.7448961
GTACATG169900.023.3753221
TACATGG173350.022.4486712
ACATGGG168750.022.160683
ATCAACG261100.021.7019183
TCAACGC265550.021.3918574
TATCAAC267000.021.3467792
CAACGCA268150.021.184445
AACGCAG272000.020.8967466
GAGTACT154500.020.25242812-13
AGTACTT159300.019.79110112-13
GTACTTT162500.019.19699114-15
CATGGGG89800.018.7608574
AGAGTAC256600.018.637810-11
ACGCAGA305800.018.5085687
GCGTACG650.00761900618.24754152-53
CGCAGAG309950.018.184228
TACCTGG31300.018.0427722
ATTCGCG8600.017.9541480-81
Produced by FastQC (version 0.11.5)