##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938866_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4151080 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.061588309548355 33.0 31.0 34.0 30.0 34.0 2 32.26753037763666 34.0 31.0 34.0 30.0 34.0 3 32.345846382146334 34.0 31.0 34.0 30.0 34.0 4 35.96667758751939 37.0 35.0 37.0 35.0 37.0 5 35.866748171560175 37.0 35.0 37.0 35.0 37.0 6 35.896902492845236 37.0 35.0 37.0 35.0 37.0 7 35.81450128641221 37.0 35.0 37.0 33.0 37.0 8 35.79537831118649 37.0 35.0 37.0 33.0 37.0 9 37.44633685691434 39.0 37.0 39.0 34.0 39.0 10-11 37.36903986432446 39.0 37.0 39.0 34.0 39.0 12-13 37.2829003536429 39.0 37.0 39.0 33.5 39.0 14-15 38.50627692070498 40.0 38.0 41.0 34.0 41.0 16-17 38.42320685219268 40.0 38.0 41.0 33.0 41.0 18-19 38.562462660319724 40.0 38.0 41.0 34.0 41.0 20-21 38.56302925985527 40.0 38.0 41.0 34.0 41.0 22-23 38.49219576592116 40.0 38.0 41.0 34.0 41.0 24-25 38.409630987598405 40.0 38.0 41.0 34.0 41.0 26-27 38.23522480896538 40.0 38.0 41.0 33.0 41.0 28-29 38.06138209815277 40.0 37.5 41.0 33.0 41.0 30-31 37.82946703026682 40.0 37.0 41.0 33.0 41.0 32-33 37.683129089297246 40.0 37.0 41.0 32.5 41.0 34-35 37.653942829335975 40.0 37.0 41.0 32.5 41.0 36-37 37.5369305096505 40.0 37.0 41.0 32.0 41.0 38-39 37.41578553051254 40.0 36.5 41.0 32.0 41.0 40-41 37.231867489906236 40.0 36.0 41.0 31.0 41.0 42-43 37.02815460073042 40.0 36.0 41.0 31.0 41.0 44-45 36.784480544822074 39.5 36.0 41.0 30.5 41.0 46-47 36.46956767877275 39.0 35.0 41.0 30.0 41.0 48-49 36.160295031654414 39.0 35.0 41.0 29.5 41.0 50-51 35.911592886670455 39.0 35.0 41.0 28.5 41.0 52-53 35.66683549823179 39.0 35.0 40.0 28.0 41.0 54-55 35.37376803145206 38.0 34.5 40.0 27.5 41.0 56-57 35.09226803627008 38.0 34.0 40.0 27.0 41.0 58-59 34.76767419563102 38.0 34.0 40.0 26.0 41.0 60-61 34.39130286576024 37.0 34.0 40.0 26.0 41.0 62-63 34.020103442959424 37.0 33.0 40.0 25.5 41.0 64-65 33.6118881110458 36.0 33.0 39.0 24.5 41.0 66-67 33.19981534925851 36.0 32.0 39.0 23.5 40.0 68-69 32.85156609846112 35.0 32.0 38.5 22.5 40.0 70-71 32.402881659712655 35.0 32.0 38.0 22.0 40.0 72-73 31.917295980804994 35.0 31.0 37.0 20.5 39.0 74-75 31.42682627171724 35.0 31.0 36.5 19.5 39.0 76-77 29.51357092612043 32.5 28.5 35.0 17.0 37.0 78-79 30.47330911473641 34.0 30.0 35.5 17.5 37.5 80-81 30.52869168987348 34.0 30.0 35.0 17.5 37.0 82-83 30.20753864054656 34.0 30.0 35.0 13.5 37.0 84-85 29.806139968393765 34.0 30.0 35.0 7.5 36.0 86-87 29.21658893589138 34.0 29.0 35.0 3.5 36.0 88-89 28.611814636191063 34.0 29.0 35.0 2.0 35.5 90-91 28.088111768503616 33.0 27.5 35.0 2.0 35.0 92-93 27.774680565057768 33.0 27.0 35.0 2.0 35.0 94-95 27.406827138961425 33.0 27.0 35.0 2.0 35.0 96-97 26.765312761980013 33.0 25.5 35.0 2.0 35.0 98-99 25.883201961899072 33.0 24.5 34.5 2.0 35.0 100-101 22.118879785501605 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04863727555964914 1101 2 0.105159534444482 1101 3 -0.005603021330365721 1101 4 0.09444396197146432 1101 5 -0.05704057142585839 1101 6 0.030912043758966945 1101 7 0.17535177718320938 1101 8 0.19065048015004038 1101 9 0.13189242482440022 1101 10-11 0.22294450802906596 1101 12-13 0.14926420138478846 1101 14-15 0.2469652130703821 1101 16-17 0.29898601858069895 1101 18-19 0.18843028264508632 1101 20-21 0.1906623233672633 1101 22-23 0.1655644793229385 1101 24-25 0.17955914675169993 1101 26-27 0.02622848714710102 1101 28-29 0.12891648153612323 1101 30-31 0.1737286199831729 1101 32-33 0.23924511087955835 1101 34-35 0.35394300807435997 1101 36-37 0.20270114928015204 1101 38-39 0.3043962301947616 1101 40-41 0.23050542102194527 1101 42-43 0.33414767066037143 1101 44-45 0.3875183705304863 1101 46-47 0.449788308830513 1101 48-49 0.3623042887428696 1101 50-51 0.27579246780226896 1101 52-53 0.15348909038456782 1101 54-55 0.19237790012103773 1101 56-57 0.08667647692499969 1101 58-59 0.1456959734043224 1101 60-61 0.24032543340997137 1101 62-63 0.037223018621538984 1101 64-65 0.0177082373690709 1101 66-67 0.02627057214171913 1101 68-69 0.07458255530539049 1101 70-71 0.16486385054358976 1101 72-73 0.06745128141875512 1101 74-75 -0.13687905666524713 1101 76-77 -0.11122881522293326 1101 78-79 -0.2622723698798488 1101 80-81 -0.10531013311928916 1101 82-83 0.07496889572936638 1101 84-85 0.2079150305762667 1101 86-87 0.19952646205819136 1101 88-89 0.16386483878403624 1101 90-91 -0.024518457672339622 1101 92-93 0.09473781071728027 1101 94-95 0.10415196325980247 1101 96-97 0.05260022147427179 1101 98-99 -0.028376825033394226 1101 100-101 0.559702824831195 1102 1 0.2668328407373295 1102 2 0.1872854002807074 1102 3 0.19346752516683097 1102 4 0.13287637153891296 1102 5 -0.07088784897954525 1102 6 0.014069440874678207 1102 7 0.09734068425558462 1102 8 0.06154765239068638 1102 9 0.1406850908826982 1102 10-11 0.20256596826397555 1102 12-13 0.235049715551213 1102 14-15 0.2601038986134867 1102 16-17 0.31628708492849 1102 18-19 0.261620658734806 1102 20-21 0.3450530608855331 1102 22-23 0.27467734446167924 1102 24-25 0.25835447470734607 1102 26-27 0.24569121678467098 1102 28-29 0.2662888641668246 1102 30-31 0.31529573606376715 1102 32-33 0.28761697840088374 1102 34-35 0.33745801129696673 1102 36-37 0.43716888609402815 1102 38-39 0.3712073209118074 1102 40-41 0.39263650012427576 1102 42-43 0.4142546047878568 1102 44-45 0.4371408232202114 1102 46-47 0.4247525424631178 1102 48-49 0.42953878462994055 1102 50-51 0.4205070587531523 1102 52-53 0.3160896858113915 1102 54-55 0.3870520560221067 1102 56-57 0.4458762106431564 1102 58-59 0.4080656791702566 1102 60-61 0.4540662350693765 1102 62-63 0.38785010729564817 1102 64-65 0.46030192245510193 1102 66-67 0.46379329670880054 1102 68-69 0.4971034964472949 1102 70-71 0.45604203256441167 1102 72-73 0.4414731320038783 1102 74-75 0.36376943541365137 1102 76-77 0.2616564903898251 1102 78-79 0.31922238910675915 1102 80-81 0.3715943957214236 1102 82-83 0.5298790946877752 1102 84-85 0.557441553643244 1102 86-87 0.5928954247494893 1102 88-89 0.5253068641102843 1102 90-91 0.4909737388926594 1102 92-93 0.6533645456998372 1102 94-95 0.7323545009506347 1102 96-97 0.7427781656283123 1102 98-99 0.8302793612183663 1102 100-101 1.5932619013370264 1103 1 0.21525891412866827 1103 2 0.1959650272274871 1103 3 0.17979095782565224 1103 4 0.032449023774546504 1103 5 -0.03975294886680558 1103 6 0.05761169034022373 1103 7 0.0687567961692892 1103 8 0.10602834707808029 1103 9 0.19390999223152505 1103 10-11 0.24245753779010926 1103 12-13 0.17471521075697183 1103 14-15 0.21208726627791918 1103 16-17 0.19768604243061816 1103 18-19 0.07936339150587202 1103 20-21 0.1548418670909868 1103 22-23 0.18286246640574433 1103 24-25 0.2187657217291772 1103 26-27 0.20224827279262314 1103 28-29 0.1458049547692184 1103 30-31 0.226664043126938 1103 32-33 0.23191431201776425 1103 34-35 0.2485546303676287 1103 36-37 0.2792310364784356 1103 38-39 0.31641225443945586 1103 40-41 0.33815980031537407 1103 42-43 0.30058187257534996 1103 44-45 0.3404354196768722 1103 46-47 0.4114031223606318 1103 48-49 0.38422237745098187 1103 50-51 0.326923772844367 1103 52-53 0.25908228259684307 1103 54-55 0.2377501287481536 1103 56-57 0.1802376662518057 1103 58-59 0.2629054359381158 1103 60-61 0.3980935518354798 1103 62-63 0.34653294056860773 1103 64-65 0.3657019592315507 1103 66-67 0.34374408243432697 1103 68-69 0.3440107399233554 1103 70-71 0.37210287730560054 1103 72-73 0.460366720609116 1103 74-75 0.49690587048424106 1103 76-77 0.38550477632523794 1103 78-79 0.21789007560419904 1103 80-81 0.4534550755237561 1103 82-83 0.5600483864443078 1103 84-85 0.644173554965203 1103 86-87 0.5456003574274035 1103 88-89 0.3739486394134417 1103 90-91 0.4269622124332706 1103 92-93 0.46006021016901855 1103 94-95 0.5976614681614763 1103 96-97 0.5953195926655255 1103 98-99 0.5187387452797054 1103 100-101 1.2478006203315424 1104 1 0.15533149390174827 1104 2 0.11853545805698928 1104 3 0.14280556117007848 1104 4 0.1276432495972486 1104 5 -0.1120897219005883 1104 6 0.005916953879136599 1104 7 0.08540754285600372 1104 8 0.05295741846494906 1104 9 0.10279873102293635 1104 10-11 0.10991090630638922 1104 12-13 0.011649690163480386 1104 14-15 0.09794318066794006 1104 16-17 0.18444425941240894 1104 18-19 0.20412553618032092 1104 20-21 0.19406881233455664 1104 22-23 0.16105490350001617 1104 24-25 0.12352430540987314 1104 26-27 0.09637244705759684 1104 28-29 0.14041441309787217 1104 30-31 0.0671514538638931 1104 32-33 0.1410366916907364 1104 34-35 0.2723613901891966 1104 36-37 0.35496569440822157 1104 38-39 0.21911861532373678 1104 40-41 0.44228887292006647 1104 42-43 0.4043737332802806 1104 44-45 0.37927441055122557 1104 46-47 0.47357259860024925 1104 48-49 0.41011304592213094 1104 50-51 0.29896932356986383 1104 52-53 0.30419059014266736 1104 54-55 0.2555617503748664 1104 56-57 0.2506963195398981 1104 58-59 0.42224465509721654 1104 60-61 0.48635539786230453 1104 62-63 0.5245076981070014 1104 64-65 0.2550174388082169 1104 66-67 0.17010191426950172 1104 68-69 0.2460029287403671 1104 70-71 0.23495435488684535 1104 72-73 0.3552724416646811 1104 74-75 0.38825468538259145 1104 76-77 0.21896748416216028 1104 78-79 0.10035478619001381 1104 80-81 0.29001355323163125 1104 82-83 0.3547668508734425 1104 84-85 0.22306856665344554 1104 86-87 0.4436134627413715 1104 88-89 0.49572800078225043 1104 90-91 0.4528990001710973 1104 92-93 0.4953660310857657 1104 94-95 0.46847686356201734 1104 96-97 0.5628798473746812 1104 98-99 0.8622884922382852 1104 100-101 1.451423159053963 1105 1 0.07271510549623628 1105 2 0.06414480620995278 1105 3 0.060491631334762985 1105 4 0.01866919301676262 1105 5 -0.031410077636621736 1105 6 -0.04818966995258478 1105 7 -6.876302011704638E-5 1105 8 0.07818632485945187 1105 9 0.10818253745471651 1105 10-11 0.050574283411556564 1105 12-13 0.056024597050779334 1105 14-15 0.14217272752422616 1105 16-17 0.13789098161501556 1105 18-19 0.12599824950246585 1105 20-21 0.10650700140967473 1105 22-23 0.03541359149902945 1105 24-25 0.05148387403835386 1105 26-27 0.13243084032131236 1105 28-29 0.09045208602968557 1105 30-31 0.012294827124975427 1105 32-33 0.12094272438434217 1105 34-35 0.16528107312222318 1105 36-37 0.11320897948787945 1105 38-39 0.1595381715466857 1105 40-41 0.08343488921048703 1105 42-43 0.2427727082781388 1105 44-45 0.2617333274182343 1105 46-47 0.2424658371976065 1105 48-49 0.1582118464221196 1105 50-51 0.18304354692492808 1105 52-53 0.17507989074479724 1105 54-55 0.2270747497304555 1105 56-57 0.21762537660418246 1105 58-59 0.17958237461005666 1105 60-61 0.07851832001799863 1105 62-63 0.17687660584869036 1105 64-65 0.05029165803834701 1105 66-67 0.0033724776674333157 1105 68-69 0.08106591845282907 1105 70-71 0.10793952772485937 1105 72-73 0.15890063900940987 1105 74-75 0.2486663800962461 1105 76-77 0.31648481162638475 1105 78-79 -0.0171931535482166 1105 80-81 0.21202537336082372 1105 82-83 0.32735973812737384 1105 84-85 0.2705281779574342 1105 86-87 0.35786601926777095 1105 88-89 0.3358174306615602 1105 90-91 0.295823444330793 1105 92-93 0.4774255863520871 1105 94-95 0.7569191064815612 1105 96-97 0.8635335945149265 1105 98-99 1.0255611575971173 1105 100-101 1.5897439797226909 1106 1 0.17814707865871782 1106 2 0.09388357951272752 1106 3 0.03577894857379249 1106 4 0.08018745104138247 1106 5 -0.017042528969533066 1106 6 0.018166141317351503 1106 7 0.08045106659485413 1106 8 0.13109724754631458 1106 9 0.09755660541060251 1106 10-11 0.140856955489987 1106 12-13 0.17949510764361776 1106 14-15 0.1786699819264186 1106 16-17 0.2095556358249766 1106 18-19 0.05700144355238024 1106 20-21 0.07788512819176674 1106 22-23 0.16215924820693317 1106 24-25 0.23047452213866393 1106 26-27 0.23897869618479461 1106 28-29 0.29930785044641084 1106 30-31 0.23692215673325023 1106 32-33 0.20029880070955386 1106 34-35 0.30587921452251265 1106 36-37 0.24078032353229872 1106 38-39 0.25314284294508127 1106 40-41 0.2849749406686968 1106 42-43 0.3090095659541561 1106 44-45 0.3361942879892581 1106 46-47 0.4808109016809965 1106 48-49 0.4421468463016396 1106 50-51 0.3468969419081631 1106 52-53 0.3959824976571156 1106 54-55 0.45450633735799073 1106 56-57 0.5017248981660316 1106 58-59 0.47954229503992707 1106 60-61 0.491609078691873 1106 62-63 0.5282039437566795 1106 64-65 0.532782106677864 1106 66-67 0.45921298400332233 1106 68-69 0.45827076334194317 1106 70-71 0.5102136216508057 1106 72-73 0.6409524881046096 1106 74-75 0.7552935651289232 1106 76-77 0.658937911568291 1106 78-79 0.5563870203173344 1106 80-81 0.4598496443918876 1106 82-83 0.6681063908005491 1106 84-85 0.7502924394036725 1106 86-87 0.7593576378987485 1106 88-89 0.8653706652729589 1106 90-91 0.6400437936147441 1106 92-93 0.8434908274319071 1106 94-95 1.0550106817398515 1106 96-97 1.215665147604966 1106 98-99 1.4650080755932962 1106 100-101 1.951941555671734 1107 1 -0.04202494682913027 1107 2 -0.027862760206012638 1107 3 -0.0484119440521269 1107 4 -0.05604129956199699 1107 5 -0.07539549988493377 1107 6 -0.09836043362210489 1107 7 -0.0015547231640198333 1107 8 -0.034462964735880064 1107 9 0.0336863411873054 1107 10-11 -0.1609359873505909 1107 12-13 -0.04324065426902024 1107 14-15 -0.054007842060073585 1107 16-17 -0.06715813145790861 1107 18-19 -0.039195897676322033 1107 20-21 -0.12587254095296174 1107 22-23 -0.07429047057428306 1107 24-25 -0.10386974665890136 1107 26-27 -0.1419874647855579 1107 28-29 -0.05870959412666821 1107 30-31 -0.0648743089053454 1107 32-33 -0.12906842678117414 1107 34-35 -0.11988997228931453 1107 36-37 -0.18327775154094184 1107 38-39 -0.17535578625621895 1107 40-41 -0.19829354360039986 1107 42-43 -0.24303840326034276 1107 44-45 -0.18517556949877445 1107 46-47 -0.3130123694924336 1107 48-49 -0.2692748985052589 1107 50-51 -0.30363617037632906 1107 52-53 -0.2646626315367939 1107 54-55 -0.13258333398646727 1107 56-57 -0.20324918506612022 1107 58-59 -0.1232523863418109 1107 60-61 -0.11311206226442039 1107 62-63 -0.09572247010217438 1107 64-65 -0.13397553423069297 1107 66-67 -0.1846843331574135 1107 68-69 -0.14366435532391364 1107 70-71 -0.1444571648071289 1107 72-73 -0.13263263116630597 1107 74-75 -0.018923721590528686 1107 76-77 -0.14699784173904007 1107 78-79 -0.012805116044532383 1107 80-81 0.10060545936163123 1107 82-83 0.035602313363533256 1107 84-85 0.018006721660469793 1107 86-87 0.05825900896635616 1107 88-89 0.0037954636196637637 1107 90-91 0.24847664599202446 1107 92-93 0.3747966963865075 1107 94-95 0.385647541770485 1107 96-97 0.5318979857297066 1107 98-99 0.6512531628363334 1107 100-101 1.3180666526495237 1108 1 0.11085493530143253 1108 2 0.004203007396093028 1108 3 -0.001551837852204585 1108 4 0.003075632238989101 1108 5 -0.0348475206344645 1108 6 -1.7956033705956997E-4 1108 7 0.028989456242378253 1108 8 0.0668770963139238 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-1.6113647339545771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 15.0 4 61.0 5 135.0 6 302.0 7 721.0 8 1619.0 9 3005.0 10 4813.0 11 7174.0 12 9808.0 13 13532.0 14 17096.0 15 21956.0 16 28153.0 17 36415.0 18 35036.0 19 26476.0 20 24977.0 21 26743.0 22 27611.0 23 31235.0 24 36744.0 25 42914.0 26 51903.0 27 63071.0 28 76663.0 29 93146.0 30 112706.0 31 136490.0 32 167666.0 33 211738.0 34 280430.0 35 392876.0 36 587221.0 37 871522.0 38 654640.0 39 54455.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.742124569190224 19.224891333192797 14.660462861076724 23.37252123654025 2 16.09682299546142 20.54503888144772 38.9450938069129 24.41304431617796 3 19.695452749645874 24.009342147103887 29.740260365977043 26.55494473727319 4 13.569986605895334 16.84477292656369 35.734941268296446 33.85029919924453 5 14.095619196516138 37.11410311339273 33.716629741886386 15.073647948204744 6 32.85156818398638 35.69489240447169 16.737847742522373 14.715691669019563 7 29.046898638426626 32.54873430528923 20.109513668731992 18.294853387552156 8 26.697726856625266 34.973476781945905 19.172938126945276 19.155858234483556 9 26.54282740877073 16.79350915906222 19.910697938849648 36.752965493317404 10-11 25.573007506480238 26.27681229944978 26.593296202434065 21.55688399163591 12-13 26.43291625311967 23.492609152316987 27.363505401004073 22.710969193559265 14-15 22.954291413318945 24.983245324108424 25.807970455881364 26.25449280669127 16-17 22.710925478127063 28.43016529298928 26.286180614736942 22.57272861414672 18-19 22.509925127918518 26.570615357930947 28.796096437553604 22.123363076596934 20-21 23.123946057411565 25.648397525463253 28.982168495909498 22.245487921215684 22-23 22.854895593435927 25.722426452874913 28.93724042899679 22.48543752469237 24-25 22.886869834682123 25.978246297605423 28.869878767212303 22.265005100500147 26-27 22.593577444810645 26.24902222992045 28.71736319710761 22.440037128161297 28-29 22.31711988205479 26.44712942174085 28.883639920213533 22.352110775990827 30-31 22.443930254295267 26.045739903832256 28.862163581525774 22.648166260346706 32-33 22.22407180781869 26.243748614818312 28.839326151266658 22.69285342609634 34-35 23.043845974918277 26.14036383181376 28.478327567163998 22.33746262610396 36-37 22.94242852537398 25.9229212737034 28.39542120985342 22.739228991069197 38-39 22.568776017937108 26.172870173294697 28.68769850008505 22.570655308683143 40-41 22.820783270769937 25.9608506758943 28.760989350530014 22.45737670280575 42-43 22.63557823424257 26.211000929954658 28.297869777437278 22.8555510583655 44-45 22.72231544129427 26.295925115305902 28.168199059725385 22.813560383674442 46-47 22.928377037462987 26.512319633288584 27.700233940415657 22.859069388832772 48-49 23.05980580110537 26.651805997684413 27.497433149875622 22.790955051334592 50-51 22.785755892806637 26.74693176085826 27.56326188040793 22.904050465927163 52-53 23.205274290064274 26.659218805708395 27.352171001281594 22.78333590294574 54-55 22.66529433304104 26.559425498906307 27.55098673116394 23.22429343688871 56-57 22.83677890221034 26.912235318831556 27.32633716145768 22.92464861750042 58-59 23.107396290658734 26.469091452654087 27.359273612128753 23.064238644558422 60-61 22.821362945671165 26.48588975234117 27.844342500462243 22.848404801525426 62-63 22.719040394233925 26.585772040631767 27.8045756266264 22.89061193850791 64-65 22.52530312741319 26.574730996742126 27.56912550498149 23.3308403708632 66-67 22.481859743941666 26.907590756690613 27.27920130113078 23.331348198236938 68-69 22.540259173984555 26.582636174145357 27.669367374335184 23.2077372775349 70-71 22.559417095992895 26.652992307058554 27.420877179373647 23.366713417574907 72-73 22.62648515567033 26.575240660261905 27.5083231351841 23.289951048883665 74-75 22.472969541740927 26.921546266036273 27.212026427961256 23.393457764261544 76-77 22.583978225700214 26.703961530752657 27.347847524460594 23.364212719086535 78-79 22.63855765236384 26.76895582935809 27.31029937456004 23.282187143718026 80-81 22.57619698805129 27.00286567188629 27.21151250646375 23.209424833598668 82-83 22.607160468904063 26.901011095770915 27.118378257405794 23.373450177919224 84-85 22.54574713086715 26.819080817522188 27.053031982038412 23.582140069572255 86-87 22.670369769255053 26.680859776400645 27.182896016580276 23.465874437764025 88-89 22.51636072295061 26.821540561864314 26.894068194590186 23.768030520594884 90-91 22.548601351500057 26.797608501279257 27.11432173595275 23.539468411267933 92-93 22.6435109691286 26.571002724846842 27.13652106607013 23.648965239954432 94-95 22.43912374765557 26.404985998169867 27.173463577836483 23.982426676338083 96-97 22.540474455412152 26.275868299175514 27.193004221797967 23.990653023614364 98-99 22.31834847798645 26.392962795224374 26.896988253659288 24.391700473129884 100-101 22.45366271656367 25.82342796283622 27.581738127226707 24.141171193373406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1881.0 1 1266.0 2 784.5 3 1006.0 4 921.5 5 897.5 6 1596.0 7 3281.5 8 5078.0 9 5819.0 10 5056.0 11 3958.5 12 3797.5 13 4063.5 14 4519.0 15 4874.5 16 5129.5 17 5471.0 18 5815.5 19 6212.0 20 6879.5 21 8154.5 22 9793.0 23 11642.5 24 14021.0 25 17326.0 26 21446.0 27 26297.0 28 31184.0 29 36397.0 30 42925.5 31 50152.5 32 58255.0 33 64320.0 34 70256.0 35 76048.0 36 82266.5 37 90810.5 38 96890.0 39 100322.0 40 104776.5 41 114204.5 42 123543.5 43 130071.0 44 136345.0 45 143377.5 46 146776.0 47 152993.0 48 170155.0 49 172972.5 50 163908.5 51 159201.5 52 152184.5 53 151867.5 54 153776.0 55 144415.0 56 131343.0 57 119132.0 58 107773.5 59 95186.5 60 80929.0 61 67423.0 62 56499.0 63 45536.0 64 34663.5 65 27124.0 66 20125.0 67 15918.0 68 11552.0 69 7147.5 70 5548.0 71 4426.5 72 3515.0 73 2941.5 74 2365.0 75 1967.0 76 1342.5 77 737.0 78 473.0 79 322.5 80 229.0 81 158.5 82 100.0 83 57.5 84 33.0 85 24.5 86 19.5 87 9.5 88 9.0 89 5.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.234902724110352 2 0.0 3 0.0 4 0.0 5 0.028956319801111997 6 0.003950779074361371 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.00283058866608208 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.974869190668452E-4 26-27 0.001276776164275321 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0069499985545930214 36-37 0.01748942443894119 38-39 0.013924087225493124 40-41 0.008600171521628105 42-43 0.008190639544407722 44-45 0.003974869190668453 46-47 5.540726750628751E-4 48-49 0.0018790290719523594 50-51 4.818023261416306E-5 52-53 0.0 54-55 0.0 56-57 0.0011563255827399135 58-59 4.6975726798808984E-4 60-61 0.0025896875030112644 62-63 0.0010117848848974244 64-65 0.0014815421528855141 66-67 0.005516636634321671 68-69 1.8067587230311148E-4 70-71 4.818023261416306E-5 72-73 0.0 74-75 5.299825587557936E-4 76-77 0.060695048035691916 78-79 0.14063809900074198 80-81 0.001120190408279291 82-83 9.395145359761797E-4 84-85 0.0 86-87 0.1076105495437332 88-89 0.3547390076799291 90-91 0.39187392196729526 92-93 5.058924424487122E-4 94-95 3.7339680275976373E-4 96-97 1.2045058153540767E-4 98-99 0.0 100-101 0.001180415699046995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4151080.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.669663946145405 #Duplication Level Percentage of deduplicated Percentage of total 1 67.82111518330947 28.939041933246358 2 15.988442482688356 13.644429355171738 3 6.453408449759471 8.260943095753554 4 3.1464938202070005 5.3703933566742394 5 1.8490580350895798 3.944934248709616 6 1.1070584759905358 2.8342687883548825 7 0.7464583322168162 2.22957883298442 8 0.503508294656486 1.718762378167122 9 0.3496002781814921 1.3425593746934896 >10 1.7929374635649709 13.49125598738452 >50 0.12938458407959014 3.8395842645091904 >100 0.10020717269222891 8.413101665081001 >500 0.0078036010127595905 2.2713376675401022 >1k 0.0043541830556614895 2.8151277056552955 >5k 5.6547831891707476E-5 0.20420177913072413 >10k+ 1.1309566378341495E-4 0.6804795669437176 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14948 0.36009905855825475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13254 0.3192904015340586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8463 0.203874654306831 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4777 0.11507848559892846 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.2045058153540766E-5 0.0 18-19 0.0 0.0 0.0 7.22703489212446E-5 0.0 20-21 0.0 0.0 0.0 1.6863081414957072E-4 0.0 22-23 0.0 0.0 0.0 3.7339680275976373E-4 0.0 24-25 0.0 0.0 0.0 6.383880821376605E-4 0.0 26-27 0.0 0.0 0.0 0.001180415699046995 0.0 28-29 0.0 0.0 0.0 0.0032521657014560066 0.0 30-31 0.0 0.0 0.0 0.009985353209285295 2.409011630708153E-5 32-33 0.0 0.0 0.0 0.02248812357266061 2.409011630708153E-5 34-35 0.0 0.0 0.0 0.04306108289890824 2.409011630708153E-5 36-37 0.0 0.0 0.0 0.07832901317247559 2.409011630708153E-5 38-39 0.0 0.0 0.0 0.129568690557638 2.409011630708153E-5 40-41 0.0 0.0 0.0 0.1844339304470162 2.409011630708153E-5 42-43 0.0 0.0 0.0 0.2422020293513977 2.409011630708153E-5 44-45 0.0 0.0 0.0 0.3170379756593465 2.409011630708153E-5 46-47 0.0 0.0 0.0 0.41121828536188176 2.409011630708153E-5 48-49 0.0 0.0 0.0 0.5116740703624116 2.409011630708153E-5 50-51 0.0 0.0 0.0 0.6191400792083024 2.409011630708153E-5 52-53 0.0 0.0 0.0 0.7266783584031143 2.409011630708153E-5 54-55 0.0 0.0 0.0 0.8485141216261791 2.409011630708153E-5 56-57 0.0 0.0 0.0 0.9943436406910973 2.409011630708153E-5 58-59 0.0 0.0 0.0 1.1549042658777957 2.409011630708153E-5 60-61 0.0 0.0 0.0 1.330942790791794 2.409011630708153E-5 62-63 0.0 0.0 0.0 1.5097878142555672 2.409011630708153E-5 64-65 0.0 0.0 0.0 1.6981966138932518 2.409011630708153E-5 66-67 0.0 0.0 0.0 1.920512252233154 2.409011630708153E-5 68-69 0.0 0.0 0.0 2.171410813571408 2.409011630708153E-5 70-71 0.0 0.0 0.0 2.4459899592395233 2.409011630708153E-5 72-73 0.0 0.0 0.0 2.7224360889214374 2.409011630708153E-5 74-75 0.0 0.0 0.0 3.0072294439037552 2.409011630708153E-5 76-77 0.0 0.0 0.0 3.319846883220752 2.409011630708153E-5 78-79 0.0 0.0 0.0 3.674959287703441 2.409011630708153E-5 80-81 0.0 0.0 0.0 4.057161027973443 2.409011630708153E-5 82-83 2.409011630708153E-5 0.0 0.0 4.442904015340586 2.409011630708153E-5 84-85 2.409011630708153E-5 0.0 0.0 4.8380662381837976 2.409011630708153E-5 86-87 2.409011630708153E-5 0.0 0.0 5.250019272093045 2.409011630708153E-5 88-89 2.409011630708153E-5 0.0 0.0 5.694770999354384 2.409011630708153E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15365 0.0 29.840702 1 GTATCAA 21465 0.0 26.744896 1 GTACATG 16990 0.0 23.375322 1 TACATGG 17335 0.0 22.448671 2 ACATGGG 16875 0.0 22.16068 3 ATCAACG 26110 0.0 21.701918 3 TCAACGC 26555 0.0 21.391857 4 TATCAAC 26700 0.0 21.346779 2 CAACGCA 26815 0.0 21.18444 5 AACGCAG 27200 0.0 20.896746 6 GAGTACT 15450 0.0 20.252428 12-13 AGTACTT 15930 0.0 19.791101 12-13 GTACTTT 16250 0.0 19.196991 14-15 CATGGGG 8980 0.0 18.760857 4 AGAGTAC 25660 0.0 18.6378 10-11 ACGCAGA 30580 0.0 18.508568 7 GCGTACG 65 0.007619006 18.247541 52-53 CGCAGAG 30995 0.0 18.18422 8 TACCTGG 3130 0.0 18.042772 2 ATTCGCG 860 0.0 17.95414 80-81 >>END_MODULE