FastQCFastQC Report
Thu 26 May 2016
SRR938865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194246
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35091.8064722053478577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27461.4136713239912275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21171.0898551321520134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.9575486753909991No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.7433872512175284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.3130051584073803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.2625536690588223No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.2620388579430207No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3860.1987170906994224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.17194691267773854No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.13333607899261762No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2490.12818796783460149No Hit
GTACATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA2160.11119920101314827No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.10193260092871927No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT459.652388E-442.1802251
ATATAGG459.7262376E-442.114982
TCTATAC757.0139376E-637.903483
GGTATCA18400.036.6211431
GTATCAA27900.033.6761441
CATGGGG4150.029.6834474
GATTTAG650.005860668629.2016931
GGTTAGA650.005860668629.2016931
GAGTACC505.048948E-528.4129612-13
TATCAAC33900.027.5331332
ATGGGGG3500.027.0739145
CTATACT700.00847921927.0739124
ATCAACG34650.026.9371783
TCAACGC35450.026.3292874
CAACGCA35900.025.9992525
ATGGGCA1109.60144E-525.843285
AACGCAG36450.025.7170436
ATGGGAG4650.025.472775
TACATGG18950.024.7522742
AACTGCT1151.3054603E-424.7005587
Produced by FastQC (version 0.11.5)