##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938865_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 194246 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97239582797072 31.0 31.0 34.0 28.0 34.0 2 30.956797051161928 33.0 31.0 34.0 27.0 34.0 3 30.8818302564789 33.0 31.0 34.0 27.0 34.0 4 34.47432122154382 37.0 35.0 37.0 32.0 37.0 5 34.51114566065711 37.0 35.0 37.0 32.0 37.0 6 34.63145187030879 37.0 35.0 37.0 32.0 37.0 7 34.55688148018492 37.0 35.0 37.0 32.0 37.0 8 34.54537030363559 37.0 35.0 37.0 32.0 37.0 9 35.999917630221475 39.0 37.0 39.0 31.0 39.0 10-11 35.89400039125645 39.0 37.0 39.0 31.0 39.0 12-13 35.75264355507964 39.0 35.0 39.0 30.0 39.0 14-15 36.68802961193538 40.0 36.5 41.0 30.0 41.0 16-17 36.61080537050956 39.0 36.5 41.0 30.0 41.0 18-19 36.64461044242867 40.0 36.0 41.0 30.0 41.0 20-21 36.58592969739402 40.0 36.0 41.0 30.0 41.0 22-23 36.407763866437406 40.0 36.0 41.0 30.0 41.0 24-25 36.29044098720179 40.0 35.5 41.0 30.0 41.0 26-27 36.10256581860116 39.0 35.0 41.0 29.0 41.0 28-29 35.879727253070854 39.0 35.0 41.0 28.5 41.0 30-31 35.42043594205286 38.5 35.0 41.0 27.0 41.0 32-33 34.90174315043811 38.0 35.0 40.0 25.5 41.0 34-35 34.40746527599023 38.0 34.5 40.0 23.5 41.0 36-37 34.00206439257437 38.0 34.0 40.0 21.0 41.0 38-39 33.663125624208476 38.0 34.0 40.0 18.5 41.0 40-41 33.26692441543197 37.0 33.0 40.0 13.5 41.0 42-43 32.715381526517916 37.0 33.0 40.0 5.0 41.0 44-45 32.124604882468624 37.0 32.5 40.0 2.0 41.0 46-47 31.41660317329572 37.0 31.0 40.0 2.0 41.0 48-49 30.80342967165347 37.0 30.0 40.0 2.0 41.0 50-51 29.528914366318997 36.0 27.0 39.0 2.0 40.0 52-53 29.532482007351504 35.5 27.5 39.0 2.0 40.0 54-55 29.900551362705023 36.0 28.0 40.0 2.0 41.0 56-57 29.727559383462207 35.5 27.5 40.0 2.0 41.0 58-59 29.460979891477816 35.0 26.5 40.0 2.0 41.0 60-61 29.13104259547172 35.0 26.0 40.0 2.0 41.0 62-63 28.74311697538173 35.0 25.5 39.0 2.0 41.0 64-65 28.319460889799533 35.0 23.0 39.0 2.0 41.0 66-67 27.901089340321036 35.0 22.0 39.0 2.0 41.0 68-69 27.46844465265694 35.0 20.0 38.0 2.0 40.0 70-71 27.020880738856913 35.0 16.5 37.5 2.0 40.0 72-73 26.548438577885776 34.0 10.5 37.0 2.0 39.5 74-75 26.056562297293123 34.0 4.5 36.0 2.0 39.0 76-77 25.576248674361377 34.0 2.0 36.0 2.0 39.0 78-79 25.11462784304439 33.5 2.0 35.0 2.0 38.0 80-81 24.60735871008927 33.0 2.0 35.0 2.0 37.0 82-83 24.05395220493601 33.0 2.0 35.0 2.0 37.0 84-85 23.544286626236833 32.5 2.0 35.0 2.0 36.0 86-87 23.07833623343595 32.0 2.0 35.0 2.0 36.0 88-89 22.603137258939697 32.0 2.0 35.0 2.0 35.5 90-91 22.110089783058594 31.0 2.0 35.0 2.0 35.0 92-93 21.57081999114525 31.0 2.0 35.0 2.0 35.0 94-95 21.00420343276052 30.5 2.0 35.0 2.0 35.0 96-97 20.37375287007197 29.5 2.0 34.5 2.0 35.0 98-99 19.70886916590303 29.0 2.0 34.0 2.0 35.0 100-101 18.051656147359534 23.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8684525280330284 1101 2 0.42805041759464046 1101 3 0.6042701609619634 1101 4 0.8595781106634846 1101 5 0.9334234388150051 1101 6 0.5156277646221028 1101 7 0.6749604702883403 1101 8 0.9773702066250607 1101 9 0.5113533239632275 1101 10-11 0.7930044746612239 1101 12-13 0.8752672351353254 1101 14-15 1.162007148404264 1101 16-17 1.0726260011869115 1101 18-19 1.2379437745491018 1101 20-21 1.3422584746678936 1101 22-23 0.9323853616758342 1101 24-25 0.7824917184905047 1101 26-27 1.0529214912666305 1101 28-29 0.7296397425382111 1101 30-31 0.8407393658962192 1101 32-33 0.9708105684685009 1101 34-35 0.2490127344795212 1101 36-37 0.5124584015527702 1101 38-39 0.6241223272235672 1101 40-41 0.7269720603856769 1101 42-43 0.872935785798866 1101 44-45 0.6620042757519755 1101 46-47 1.2659918646178632 1101 48-49 0.8632231098605345 1101 50-51 0.3177058727920752 1101 52-53 -0.30909638469509204 1101 54-55 -0.7054979384063351 1101 56-57 -0.4183515878842705 1101 58-59 -0.25057123741427034 1101 60-61 -0.14178176374418072 1101 62-63 -0.1734920164086553 1101 64-65 -0.2153168652380728 1101 66-67 -0.2220393502015483 1101 68-69 -0.2642932512296632 1101 70-71 -0.38079693420919725 1101 72-73 -0.6911702999465241 1101 74-75 -0.4419249246146322 1101 76-77 -0.8490965871424301 1101 78-79 -0.5568249893651789 1101 80-81 -0.5589237390936077 1101 82-83 -0.49832167837922015 1101 84-85 -0.642291571442339 1101 86-87 -0.5725037259592263 1101 88-89 -0.6334460501665262 1101 90-91 -0.35376058527315735 1101 92-93 -0.5335226998725169 1101 94-95 -0.878116857531122 1101 96-97 -1.2405192717987958 1101 98-99 -0.8882596760179382 1101 100-101 -0.46723657830344933 1102 1 0.7055311797184203 1102 2 0.8662526647856517 1102 3 0.5087645429844372 1102 4 0.1517129421241563 1102 5 0.47836725903972166 1102 6 0.6560772028243491 1102 7 0.1356346275917133 1102 8 0.8481567234789935 1102 9 0.3709038857609812 1102 10-11 0.6244651488185227 1102 12-13 0.23762678569712392 1102 14-15 0.02998467649415204 1102 16-17 0.592288922535225 1102 18-19 0.5132246734255048 1102 20-21 0.1315843173645206 1102 22-23 0.31440783358593904 1102 24-25 0.3920422802882584 1102 26-27 0.3928091317160636 1102 28-29 0.7464936751224798 1102 30-31 0.6890539726378009 1102 32-33 0.9314847257718739 1102 34-35 1.305192509760417 1102 36-37 1.3776269408786135 1102 38-39 1.4134481699201942 1102 40-41 1.5078709367901766 1102 42-43 1.780239156585374 1102 44-45 1.7631278712575877 1102 46-47 1.9232952354043782 1102 48-49 1.7424365930066017 1102 50-51 1.8233238503201648 1102 52-53 1.7133755254172698 1102 54-55 1.8759627357509707 1102 56-57 2.0563675132393264 1102 58-59 1.7269568524733714 1102 60-61 1.644735090188405 1102 62-63 1.7703282083104455 1102 64-65 1.9672674044248488 1102 66-67 1.8200954812591235 1102 68-69 2.128965175736628 1102 70-71 1.4731356500604669 1102 72-73 1.1936611607276326 1102 74-75 1.0777379967336813 1102 76-77 0.5329258847676819 1102 78-79 0.6566581567022354 1102 80-81 1.2219751373108885 1102 82-83 0.8387569733061717 1102 84-85 1.0459106757486758 1102 86-87 0.8741254875239193 1102 88-89 0.9845314779233583 1102 90-91 1.2389360439403276 1102 92-93 0.9973761765319757 1102 94-95 0.9252539289857289 1102 96-97 0.9364470203360327 1102 98-99 1.030279649824756 1102 100-101 0.9456847700111588 1103 1 -0.5165852425503523 1103 2 -0.06674422469571795 1103 3 -0.5453838731902607 1103 4 -0.2853149255835774 1103 5 -0.49338415338965547 1103 6 -0.381820340500731 1103 7 -0.42517918565020807 1103 8 -0.4488660658309769 1103 9 -0.40644011220765464 1103 10-11 -0.6946645407644141 1103 12-13 -0.4630175360193718 1103 14-15 -0.48001341910668316 1103 16-17 -0.6634560149370046 1103 18-19 -0.9122180993796789 1103 20-21 -0.4093602646198633 1103 22-23 -0.723442099760085 1103 24-25 -0.9964710187658952 1103 26-27 -0.3780122991225028 1103 28-29 -0.28634134043011983 1103 30-31 -0.2931875051759576 1103 32-33 -0.37412372976041297 1103 34-35 -0.31733970632539865 1103 36-37 -0.43033027362021414 1103 38-39 -0.37068818505981227 1103 40-41 0.20218313129281285 1103 42-43 0.08859312090963556 1103 44-45 0.3592524189627895 1103 46-47 0.3471033989287875 1103 48-49 0.19850845233498404 1103 50-51 0.15672066362556691 1103 52-53 -0.10331018524101765 1103 54-55 0.4462668048171743 1103 56-57 0.4856825643407028 1103 58-59 0.6358630925546258 1103 60-61 0.3988612883728777 1103 62-63 0.48331938459432777 1103 64-65 0.3965348455471762 1103 66-67 0.6360829122246585 1103 68-69 0.6081278749050405 1103 70-71 0.6608141874819076 1103 72-73 0.7924677372527391 1103 74-75 1.2296138299083168 1103 76-77 0.7497575993617254 1103 78-79 1.0468219350301737 1103 80-81 0.8128625873204065 1103 82-83 0.3593244842564651 1103 84-85 0.5930128150237302 1103 86-87 0.7449754843499221 1103 88-89 0.2855693754666859 1103 90-91 0.1770113311871988 1103 92-93 0.04120909724548838 1103 94-95 0.008079422542511594 1103 96-97 -0.03798642091325277 1103 98-99 -0.18480636553739416 1103 100-101 0.19528802018572833 1104 1 0.42120744000803256 1104 2 0.3168625307953832 1104 3 0.0021251149966943217 1104 4 0.6446829595489021 1104 5 -0.018958944168836922 1104 6 0.45485146227277085 1104 7 0.6510164698076579 1104 8 0.597661019579462 1104 9 0.04635302353662496 1104 10-11 0.3831474777255721 1104 12-13 0.5885701252392721 1104 14-15 0.3451021432970123 1104 16-17 0.03293964656334225 1104 18-19 0.37274519256267524 1104 20-21 0.5659411040015456 1104 22-23 0.21170699839998264 1104 24-25 0.49579460859445845 1104 26-27 0.6811435666136205 1104 28-29 0.9807513209795928 1104 30-31 0.6687901980779216 1104 32-33 0.4760530127393636 1104 34-35 0.06826106711186242 1104 36-37 0.06787208898893482 1104 38-39 0.5023744452311334 1104 40-41 0.11728630661532691 1104 42-43 -0.16472268923568834 1104 44-45 -0.004927767749798306 1104 46-47 -1.24582992231959 1104 48-49 -0.5651702086517538 1104 50-51 -0.9312276127574037 1104 52-53 -1.0353927254990296 1104 54-55 -0.6828307510002212 1104 56-57 -0.8281184568381867 1104 58-59 -0.7216401552776333 1104 60-61 -0.8382757852547229 1104 62-63 -0.9023720260223094 1104 64-65 -1.050502829307053 1104 66-67 -0.6898937033830634 1104 68-69 -0.4502122562167443 1104 70-71 -0.9090670778131162 1104 72-73 -0.8619478040022841 1104 74-75 -1.0609689671550413 1104 76-77 -1.0521699513195628 1104 78-79 -0.6536675057384294 1104 80-81 -1.2394350651766466 1104 82-83 -1.2294999515270923 1104 84-85 -1.2617562112308356 1104 86-87 -1.0898533624430335 1104 88-89 -0.7804598097050715 1104 90-91 -0.3806037024996165 1104 92-93 -0.76907218013449 1104 94-95 -0.7268370401904996 1104 96-97 -0.3348231833532047 1104 98-99 -0.6722229038013907 1104 100-101 -0.6431814199786281 1105 1 0.38801356895984895 1105 2 0.5300181651640621 1105 3 0.7821533768902391 1105 4 0.6248165108497759 1105 5 0.6648957564490487 1105 6 0.5349268273222236 1105 7 0.34786609990934636 1105 8 0.16212307383121072 1105 9 0.6447293500446207 1105 10-11 0.5301093241692811 1105 12-13 0.6584074808126843 1105 14-15 0.6675232455108642 1105 16-17 0.3850495908707856 1105 18-19 0.7699633355900275 1105 20-21 1.176702789740368 1105 22-23 1.0656595073357238 1105 24-25 1.208119010799983 1105 26-27 1.097632441956506 1105 28-29 0.9909339149781005 1105 30-31 1.0509768928217582 1105 32-33 0.9723678834827609 1105 34-35 0.9804413890757857 1105 36-37 0.8523751506940656 1105 38-39 0.6123187526762024 1105 40-41 0.48766950464253966 1105 42-43 0.3440745178242466 1105 44-45 0.6661813730332184 1105 46-47 0.23567516205047667 1105 48-49 0.33118899447367767 1105 50-51 0.775818465212172 1105 52-53 0.632031286027388 1105 54-55 0.6601217856432662 1105 56-57 0.6297796340986146 1105 58-59 0.7909668658633677 1105 60-61 0.8871959389978556 1105 62-63 0.4620817159078143 1105 64-65 0.7200909475348212 1105 66-67 0.1467968842980767 1105 68-69 0.30162338147686185 1105 70-71 0.34729877516962304 1105 72-73 0.6323582556801846 1105 74-75 0.05581915176657759 1105 76-77 0.430914474160474 1105 78-79 0.14430151700962313 1105 80-81 0.32142498303482014 1105 82-83 0.21903292370496175 1105 84-85 0.3927698478974797 1105 86-87 0.18445325139420987 1105 88-89 0.30963190291026166 1105 90-91 0.526035071710016 1105 92-93 0.6327315936595319 1105 94-95 0.5267996005232511 1105 96-97 0.3800780385580751 1105 98-99 0.6989499647808053 1105 100-101 1.128603789630418 1106 1 -0.7851252366032817 1106 2 -0.49856105490684044 1106 3 -0.47012368883105893 1106 4 -0.662775526237759 1106 5 -1.1005801093813332 1106 6 -0.762373418110009 1106 7 -0.6792677970091177 1106 8 -0.5472306212874116 1106 9 -0.4602611053685237 1106 10-11 -0.6527376897385437 1106 12-13 -1.0246440599563371 1106 14-15 -0.8529839811049058 1106 16-17 -0.8513834606699788 1106 18-19 -0.4518255926892252 1106 20-21 -0.27911940824163395 1106 22-23 -0.8335223852195028 1106 24-25 -1.2437353660748443 1106 26-27 -1.1454818203832815 1106 28-29 -1.191708572068535 1106 30-31 -1.1542044543285002 1106 32-33 -1.1731870660672783 1106 34-35 -1.0855526114696232 1106 36-37 -0.9113145138818837 1106 38-39 -0.5759959459875503 1106 40-41 -0.5774927533340133 1106 42-43 -0.022096739334195092 1106 44-45 -0.42460128308895406 1106 46-47 -0.8539076031526882 1106 48-49 -1.1033351396959432 1106 50-51 -1.527385788946539 1106 52-53 -1.149043161750086 1106 54-55 -1.9494364363365548 1106 56-57 -1.9560807422308102 1106 58-59 -1.5494476419086496 1106 60-61 -1.511917777936965 1106 62-63 -1.1659520991999024 1106 64-65 -1.4683328321215718 1106 66-67 -1.4938903259555403 1106 68-69 -1.2380957349670965 1106 70-71 -1.1143865261666193 1106 72-73 -1.1108923579004006 1106 74-75 -0.7858634225448498 1106 76-77 -0.6015507562731237 1106 78-79 -0.7233832389216595 1106 80-81 -0.5572087775324022 1106 82-83 -0.6490608859487068 1106 84-85 -0.5174837654104039 1106 86-87 -0.25579763488885376 1106 88-89 -0.13338691356096888 1106 90-91 0.012768095980536742 1106 92-93 0.08894921023984281 1106 94-95 0.5019836746983266 1106 96-97 0.5769555951438399 1106 98-99 1.1005043689258827 1106 100-101 1.53169896279649 1107 1 -0.18290776398773545 1107 2 -0.2927902827989044 1107 3 -0.27244802759675224 1107 4 -0.47912604721024366 1107 5 0.0773520306133264 1107 6 -0.3213368164535808 1107 7 -0.3465133219890788 1107 8 -0.5991141371837543 1107 9 -0.47575669713982904 1107 10-11 -0.8134376630220075 1107 12-13 -0.23056177302071745 1107 14-15 -0.3399463107869778 1107 16-17 0.01650326121086465 1107 18-19 0.039945713749460765 1107 20-21 -0.12376274246551588 1107 22-23 -0.16635700985214186 1107 24-25 -0.47308348255894117 1107 26-27 -0.3113915184864027 1107 28-29 -0.055921770038075636 1107 30-31 0.10924732232124512 1107 32-33 -0.0037374267405354544 1107 34-35 0.20405465542859247 1107 36-37 0.07894730818597395 1107 38-39 -0.03299567882218213 1107 40-41 0.5790795149000303 1107 42-43 0.5213844255068452 1107 44-45 0.2008595771835573 1107 46-47 0.05899648447755368 1107 48-49 0.4568037862992469 1107 50-51 0.9770625145533245 1107 52-53 0.5484722002589955 1107 54-55 0.8179293130623222 1107 56-57 0.4508521992599732 1107 58-59 0.6373543885482036 1107 60-61 0.8775959810798639 1107 62-63 0.8957631025099566 1107 64-65 1.1265242344026056 1107 66-67 1.3040257841165968 1107 68-69 1.0252892503388082 1107 70-71 1.5281462015918628 1107 72-73 1.2937296030934569 1107 74-75 1.2337865908134162 1107 76-77 1.2197022493373488 1107 78-79 1.5672125398654124 1107 80-81 0.8201756734427512 1107 82-83 0.7668354538856512 1107 84-85 1.3408117194947522 1107 86-87 1.6346912777480682 1107 88-89 2.0586916330593894 1107 90-91 1.514331013426439 1107 92-93 1.2161065706459304 1107 94-95 1.0849385237498907 1107 96-97 0.5786788118149602 1107 98-99 0.7857835567431408 1107 100-101 0.5907825855589834 1108 1 0.5227834239369571 1108 2 0.5622576585434551 1108 3 0.33642044929202797 1108 4 0.33633335708164935 1108 5 0.2561450368175002 1108 6 0.8189688263901331 1108 7 0.7277218994542665 1108 8 0.8444708546245892 1108 9 1.0719026373215357 1108 10-11 0.6633302579083491 1108 12-13 0.8971743750199934 1108 14-15 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-1.222330607342709 2316 80-81 -1.1851035143745072 2316 82-83 -0.886136909340518 2316 84-85 -0.3733901981589405 2316 86-87 -0.7774350617894399 2316 88-89 -0.5680652761340674 2316 90-91 -0.7448342432007458 2316 92-93 -1.152161900871267 2316 94-95 -1.5748584305648308 2316 96-97 -1.5199449896514814 2316 98-99 -1.6994956310741145 2316 100-101 -1.3296460664432743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3541.0 3 694.0 4 565.0 5 559.0 6 561.0 7 634.0 8 728.0 9 819.0 10 1019.0 11 1474.0 12 2062.0 13 2708.0 14 2937.0 15 3463.0 16 4336.0 17 5417.0 18 4423.0 19 2671.0 20 2404.0 21 2405.0 22 2444.0 23 2682.0 24 2805.0 25 2923.0 26 3273.0 27 3685.0 28 4149.0 29 4672.0 30 5346.0 31 6079.0 32 6900.0 33 8369.0 34 10928.0 35 15541.0 36 17937.0 37 23938.0 38 24341.0 39 4814.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.42252431590564 20.501686384124266 16.041277732509567 18.034511567460523 2 15.784455702204195 23.31180845087843 40.28777719942898 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18.959596168566524 46-47 18.26259429070889 28.89892242884074 33.69019635223932 19.148286928211057 48-49 17.950137214481366 29.366226873346246 33.077872128001324 19.605763784171064 50-51 18.086083072777257 29.739340476319786 32.4592097198408 19.715366731062165 52-53 18.168627958101645 29.88102935471357 31.936117936117935 20.014224751066855 54-55 18.01369615028525 29.563616238834378 32.00699289848401 20.41569471239636 56-57 18.377414100674784 29.710695726363145 31.388091729362188 20.523798443599887 58-59 18.45261481833785 29.477966872764117 31.549711532496538 20.5197067764015 60-61 18.47551128509231 29.208428613878013 31.65587897109931 20.660181129930365 62-63 18.4706442087408 29.182030211667847 31.78818735164375 20.559138227947603 64-65 18.393183264438225 29.37389090946717 31.856701622958365 20.376224203136236 66-67 18.54931125913471 29.13639009247882 31.13574338744099 21.178555260945483 68-69 18.517435768744196 29.267358802482583 31.08479741703813 21.130408011735092 70-71 18.939299868583078 29.031757365038956 30.81700969587848 21.21193307049949 72-73 18.960397320158307 28.655423058020123 30.877156677617112 21.507022944204454 74-75 18.54920269518772 29.00327198892956 30.76497290586242 21.682552410020303 76-77 18.466674596976485 28.631090607237613 30.878557649042413 22.023677146743488 78-79 18.5647365400425 28.643482397224506 30.997068358435836 21.794712704297154 80-81 18.816038138277108 28.537265739924457 30.495368443367816 22.151327678430622 82-83 18.882390054197177 28.161071118282237 30.52185470191552 22.434684125605063 84-85 19.151857396619533 27.863405653766893 30.398370010678704 22.58636693893487 86-87 19.409168134178415 27.634991686255038 30.17255869400505 22.783281485561492 88-89 19.781242968246865 27.216085288861187 30.188366926254208 22.814304816637744 90-91 20.269927733031228 26.66790818939429 30.184262664825074 22.877901412749406 92-93 20.61891668886814 26.78299375858421 29.990869375160045 22.607220177387607 94-95 20.66554578375582 26.45188825659596 29.86989136057941 23.012674599068806 96-97 20.773204276265588 25.898411532362992 30.06215742927426 23.266226762097162 98-99 20.9571080872064 25.815846444727807 29.850008923421523 23.377036544644266 100-101 22.2483992096906 25.040084410009207 30.012878733022312 22.69863764727788 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1984.0 1 1258.0 2 591.5 3 594.0 4 481.5 5 456.5 6 666.0 7 1096.0 8 1528.5 9 1641.5 10 1326.5 11 996.5 12 905.0 13 922.0 14 936.5 15 914.0 16 958.0 17 1023.0 18 1028.0 19 1057.0 20 1140.0 21 1230.0 22 1348.5 23 1557.0 24 1734.0 25 1917.5 26 2281.5 27 2760.0 28 2986.0 29 3103.0 30 3398.5 31 3745.0 32 4185.5 33 4561.0 34 4817.0 35 5022.0 36 5435.5 37 5747.0 38 5526.5 39 5399.0 40 5523.5 41 5554.0 42 5417.0 43 5425.0 44 5540.0 45 5470.5 46 5439.0 47 5708.0 48 6149.5 49 5991.5 50 5487.5 51 5011.0 52 4375.0 53 3923.5 54 3585.5 55 3242.5 56 2961.0 57 2672.5 58 2432.0 59 2286.0 60 2063.5 61 1887.0 62 1635.5 63 1215.5 64 962.5 65 870.0 66 834.5 67 749.5 68 648.5 69 596.5 70 530.5 71 484.0 72 448.5 73 399.5 74 350.0 75 278.0 76 194.0 77 163.5 78 143.0 79 103.5 80 71.0 81 44.0 82 31.0 83 22.5 84 16.5 85 13.0 86 7.5 87 4.5 88 5.5 89 3.5 90 1.0 91 3.0 92 2.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17503577937254822 2 0.10656590097093376 3 0.09524005642329829 4 0.11531768993956117 5 0.13333607899261762 6 0.03552196699031126 7 0.04015526703252577 8 0.04478856707474028 9 0.04530337819054189 10-11 0.04504597263264109 12-13 0.04813483932745076 14-15 0.04530337819054189 16-17 0.04169970037993061 18-19 0.048392244885351564 20-21 0.05045148934855802 22-23 0.06666803949630881 24-25 0.08159756185455556 26-27 0.08391421187566282 28-29 0.07361798955963057 30-31 0.061777333896193484 32-33 0.0496792726748556 34-35 0.2998774749544392 36-37 0.47208179319007854 38-39 0.44273755958938665 40-41 0.4185414371467109 42-43 0.45174675411591486 44-45 0.46230038198984796 46-47 0.46049854308454224 48-49 0.48160579883240834 50-51 0.46822070982156644 52-53 0.47414103765328497 54-55 0.4669336820320624 56-57 0.43810425954717214 58-59 0.41699700379930604 60-61 0.3801880090194908 62-63 0.4514893485580141 64-65 0.49267423782214304 66-67 0.49344645449584545 68-69 0.5042574879276793 70-71 0.4983371600959608 72-73 0.4895853711273334 74-75 0.48315023217981323 76-77 0.46075594864244307 78-79 0.4319265261575528 80-81 0.4347579872944617 82-83 0.4520041596738157 84-85 0.44814307630530353 86-87 0.45895410973713746 88-89 0.4769724987901939 90-91 0.4813483932745076 92-93 0.4841798544114165 94-95 0.48701131554832533 96-97 0.4872687211062261 98-99 0.48109098771660674 100-101 0.4653892486846576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 194246.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.34662169709043 #Duplication Level Percentage of deduplicated Percentage of total 1 79.58962356873649 48.02964904516374 2 12.66416194909963 15.284787805060052 3 3.7546362425638433 6.797388388205561 4 1.4043744498729984 3.3899701459018132 5 0.780938566795504 2.3563502129538136 6 0.4501606538280814 1.6299404807686868 7 0.26171726213767005 1.1055626826874156 8 0.2061915211581731 0.9954369379583913 9 0.11399873252262746 0.619149454694172 >10 0.6889461767401335 7.315505826243234 >50 0.05452090941823296 2.2612322160036733 >100 0.022799653029442875 2.2570447489369996 >500 0.002973867786449071 0.9732869882111049 >1k 0.004956446310748452 6.984695067211347 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3509 1.8064722053478577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2746 1.4136713239912275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2117 1.0898551321520134 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1860 0.9575486753909991 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1444 0.7433872512175284 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 608 0.3130051584073803 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 510 0.2625536690588223 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.2620388579430207 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 386 0.1987170906994224 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 334 0.17194691267773854 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.13333607899261762 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 249 0.12818796783460149 No Hit GTACATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA 216 0.11119920101314827 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.10193260092871927 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.148111158016124E-4 0.0 18-19 0.0 0.0 0.0 5.148111158016124E-4 0.0 20-21 0.0 0.0 0.0 0.0015444333474048373 0.0 22-23 0.0 0.0 0.0 0.004633300042214511 0.0 24-25 0.0 0.0 0.0 0.011840655663437085 0.0 26-27 0.0 0.0 0.0 0.030888666948096742 0.0 28-29 0.0 0.0 0.0 0.07284577288592815 0.0 30-31 0.0 0.0 0.0 0.16808582930922644 0.0 32-33 0.0 0.0 0.0 0.3330827919236432 0.0 34-35 0.0 0.0 0.0 0.5693810940765833 0.0 36-37 0.0 0.0 0.0 0.8865047414103765 0.0 38-39 0.0 0.0 0.0 1.300155472956972 0.0 40-41 0.0 0.0 0.0 1.7696632105680425 0.0 42-43 0.0 0.0 0.0 2.1534548973981447 0.0 44-45 0.0 0.0 0.0 2.5920739680611184 0.0 46-47 0.0 0.0 0.0 3.0744519835672293 0.0 48-49 0.0 0.0 0.0 3.5779372548212063 0.0 50-51 0.0 0.0 0.0 4.137022126581757 0.0 52-53 0.0 0.0 0.0 4.61682608650886 0.0 54-55 0.0 0.0 0.0 5.155833324753148 0.0 56-57 0.0 0.0 0.0 5.774121474830885 0.0 58-59 0.0 0.0 0.0 6.383143024824191 0.0 60-61 0.0 0.0 0.0 7.07221770332465 0.0 62-63 0.0 0.0 0.0 7.734264798245524 0.0 64-65 0.0 0.0 0.0 8.412528443314148 0.0 66-67 0.0 0.0 0.0 9.150510177815761 0.0 68-69 0.0 0.0 0.0 9.972148718635133 0.0 70-71 0.0 0.0 0.0 10.84578318215047 0.0 72-73 0.0 0.0 0.0 11.679262378633279 0.0 74-75 0.0 0.0 0.0 12.527413691916436 0.0 76-77 0.0 0.0 0.0 13.389722310884135 0.0 78-79 0.0 0.0 0.0 14.327965569432575 0.0 80-81 0.0 0.0 0.0 15.316917722887473 0.0 82-83 0.0 0.0 0.0 16.3156512875426 0.0 84-85 0.0 0.0 0.0 17.3051182521133 0.0 86-87 0.0 0.0 0.0 18.296901866705106 0.0 88-89 0.0 0.0 0.0 19.30696127590787 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAT 45 9.652388E-4 42.180225 1 ATATAGG 45 9.7262376E-4 42.11498 2 TCTATAC 75 7.0139376E-6 37.90348 3 GGTATCA 1840 0.0 36.621143 1 GTATCAA 2790 0.0 33.676144 1 CATGGGG 415 0.0 29.683447 4 GATTTAG 65 0.0058606686 29.201693 1 GGTTAGA 65 0.0058606686 29.201693 1 GAGTACC 50 5.048948E-5 28.41296 12-13 TATCAAC 3390 0.0 27.533133 2 ATGGGGG 350 0.0 27.073914 5 CTATACT 70 0.008479219 27.073912 4 ATCAACG 3465 0.0 26.937178 3 TCAACGC 3545 0.0 26.329287 4 CAACGCA 3590 0.0 25.999252 5 ATGGGCA 110 9.60144E-5 25.84328 5 AACGCAG 3645 0.0 25.717043 6 ATGGGAG 465 0.0 25.47277 5 TACATGG 1895 0.0 24.752274 2 AACTGCT 115 1.3054603E-4 24.700558 7 >>END_MODULE