FastQCFastQC Report
Thu 26 May 2016
SRR938865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194246
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32941.6957878154505113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27591.4203638684966486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22811.174284155143478No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT21801.122288232447515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18340.9441635863801571No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.6759469950475171No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT11560.5951216498666639RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT6330.32587543630242066RNA PCR Primer, Index 33 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.3248458140708174No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.28057205811187874No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG4090.21055774636285945RNA PCR Primer, Index 33 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.20643925743644656No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3880.19974671293102564No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.1616506903617063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.15289890139307888No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2580.132821267876816No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT2310.11892136775017245RNA PCR Primer, Index 33 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2090.10759552320253699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.044.9335981
GTATTGG704.162368E-640.895791
TATAGAC608.312852E-539.544822
GTACAGG500.001577634738.1694031
ACCGAGG352.0594809E-433.9042978-79
ATAAGCC753.086677E-431.6358577
GTATCAA29000.030.9303781
TGGGATA1401.7065759E-830.5060046
ATTAGTG650.005860083729.2023289
ATGGGGC1004.9650647E-528.472275
CATGGGG4500.027.4177444
ATAGAGT1056.9225345E-527.116456
GTACATG19300.025.7099591
ATGGGAT4700.025.2413735
TATCAAC35950.025.2118852
TACATGG19400.024.9499282
CAACGCA36850.024.4673525
TCAACGC37050.024.3352744
ATCAACG37100.024.3024773
ACATGGG19050.023.913723
Produced by FastQC (version 0.11.5)