##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938865_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 194246 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.812680827404424 31.0 31.0 34.0 30.0 34.0 2 32.00589973538709 33.0 31.0 34.0 30.0 34.0 3 32.03012674649671 33.0 31.0 34.0 30.0 34.0 4 35.75213904018616 37.0 35.0 37.0 35.0 37.0 5 35.67397012036284 37.0 35.0 37.0 33.0 37.0 6 35.735263531810176 37.0 35.0 37.0 33.0 37.0 7 35.66039455123915 37.0 35.0 37.0 33.0 37.0 8 35.659946665568405 37.0 35.0 37.0 33.0 37.0 9 37.21176755248499 39.0 37.0 39.0 33.0 39.0 10-11 37.16239974053519 39.0 37.0 39.0 33.0 39.0 12-13 37.07205296376759 39.0 37.0 39.0 33.0 39.0 14-15 38.103930068058034 40.0 37.0 41.0 33.0 41.0 16-17 38.050430896903926 40.0 37.0 41.0 33.0 41.0 18-19 38.1509348969863 40.0 37.0 41.0 33.0 41.0 20-21 38.22859930191613 40.0 37.0 41.0 33.0 41.0 22-23 38.10737415442274 40.0 37.0 41.0 33.0 41.0 24-25 37.969994234115504 40.0 37.0 41.0 33.0 41.0 26-27 37.685350020077635 40.0 36.0 41.0 32.5 41.0 28-29 37.395109809211 39.5 36.0 41.0 32.0 41.0 30-31 36.91760190686037 39.0 35.0 41.0 31.5 41.0 32-33 36.357006064474945 38.0 35.0 41.0 30.5 41.0 34-35 35.9810549509385 38.0 35.0 41.0 30.0 41.0 36-37 35.6512412096002 38.0 35.0 41.0 29.5 41.0 38-39 35.30123400224458 38.0 35.0 41.0 27.5 41.0 40-41 34.94917784664807 38.0 35.0 40.0 25.5 41.0 42-43 34.50445826426284 38.0 34.0 40.0 23.5 41.0 44-45 33.866216035336635 38.0 33.5 40.0 19.5 41.0 46-47 33.0262527928503 38.0 33.0 40.0 5.5 41.0 48-49 32.3160193774904 37.5 32.5 40.0 2.0 41.0 50-51 31.81967968452375 37.0 32.0 40.0 2.0 41.0 52-53 31.513562698845792 37.0 31.0 40.0 2.0 41.0 54-55 31.198585813864895 36.5 31.0 40.0 2.0 41.0 56-57 30.794961028798532 36.0 30.5 40.0 2.0 41.0 58-59 30.34743829988777 35.0 29.5 40.0 2.0 41.0 60-61 29.851819342483243 35.0 28.5 39.0 2.0 41.0 62-63 29.33569545833634 35.0 27.5 39.0 2.0 41.0 64-65 28.851440956313127 35.0 26.0 39.0 2.0 40.0 66-67 28.285277431710306 35.0 25.5 38.0 2.0 40.0 68-69 27.74717626102983 35.0 22.5 38.0 2.0 40.0 70-71 27.272376265148317 34.0 21.5 37.0 2.0 39.5 72-73 26.952161177064134 34.0 20.5 36.5 2.0 39.0 74-75 26.54228401099637 34.0 18.5 36.0 2.0 39.0 76-77 24.68770013282127 31.5 12.5 34.5 2.0 36.5 78-79 25.538708647797122 33.0 10.5 35.0 2.0 37.0 80-81 25.51616506903617 33.0 7.0 35.0 2.0 37.0 82-83 25.185422093633846 33.0 2.0 35.0 2.0 36.5 84-85 24.787385583229515 33.0 2.0 35.0 2.0 36.0 86-87 24.247809478702266 33.0 2.0 35.0 2.0 36.0 88-89 23.73237286739495 32.0 2.0 35.0 2.0 35.0 90-91 23.29032000658958 32.0 2.0 35.0 2.0 35.0 92-93 22.960096990414215 31.5 2.0 35.0 2.0 35.0 94-95 22.559288222151295 31.0 2.0 35.0 2.0 35.0 96-97 22.073327121279203 31.0 2.0 34.0 2.0 35.0 98-99 21.42878617835116 31.0 2.0 34.0 2.0 35.0 100-101 19.027593875806968 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03590302024891301 1101 2 -0.026033552451316666 1101 3 -0.0012516506867896737 1101 4 0.07961103862280794 1101 5 0.2357095113680714 1101 6 0.20155757865268953 1101 7 -9.887141897735319E-4 1101 8 -0.13494094409141155 1101 9 -0.23788126282063615 1101 10-11 -0.1713486512576381 1101 12-13 0.05102259186036662 1101 14-15 0.006376249225908737 1101 16-17 0.025005293632197834 1101 18-19 -0.09070271095502136 1101 20-21 0.45262700378114573 1101 22-23 0.23002758824247138 1101 24-25 -0.2676040484929203 1101 26-27 -0.019473703508495532 1101 28-29 -0.15592477971725316 1101 30-31 -0.3449174964000363 1101 32-33 -0.015443143122745084 1101 34-35 -0.1346038775568772 1101 36-37 -0.3213835534714846 1101 38-39 -0.16819155012198905 1101 40-41 -0.065869319179086 1101 42-43 -0.3057161752298754 1101 44-45 -0.44369713118622656 1101 46-47 -0.6264322521012957 1101 48-49 0.041694389695635437 1101 50-51 0.14605790108115002 1101 52-53 -0.08849043088643427 1101 54-55 -0.32992952180659785 1101 56-57 -0.3705812531068773 1101 58-59 0.3568788156103153 1101 60-61 0.38174545090319256 1101 62-63 0.342791665400199 1101 64-65 0.8248271128334359 1101 66-67 0.8451668181479981 1101 68-69 -0.08158847859712992 1101 70-71 -0.06961900972113355 1101 72-73 0.3309706724028523 1101 74-75 0.33367659489953283 1101 76-77 0.24211310301341626 1101 78-79 0.5775579110207119 1101 80-81 0.30972819661941386 1101 82-83 -0.24094698966842998 1101 84-85 -0.45987435316158454 1101 86-87 -0.04090807280767095 1101 88-89 0.36655606713509314 1101 90-91 0.029888776207869228 1101 92-93 -0.07291116989154034 1101 94-95 -0.12700031007476653 1101 96-97 -0.2894446882526509 1101 98-99 -0.67940657357369 1101 100-101 -0.043824436396484145 1102 1 0.013431110136554736 1102 2 -0.0372695075074958 1102 3 -0.034959515855327084 1102 4 0.03466721839808429 1102 5 -0.07327925267686908 1102 6 -0.1130491629203405 1102 7 0.03833712850686055 1102 8 -0.12370498903523242 1102 9 0.12166929897711753 1102 10-11 0.26404460716933187 1102 12-13 0.2701237154558669 1102 14-15 0.1552526537202894 1102 16-17 0.14298282172209298 1102 18-19 0.2351399856741878 1102 20-21 -0.005238164758182506 1102 22-23 0.02216241970315025 1102 24-25 -0.042884947369323356 1102 26-27 -0.019473703508502638 1102 28-29 0.032277467473754484 1102 30-31 0.01744205416176925 1102 32-33 0.17556809283231445 1102 34-35 0.32326129098245104 1102 36-37 0.18704341282064973 1102 38-39 -0.08953986472872799 1102 40-41 0.17851270329282443 1102 42-43 -0.4461656134321288 1102 44-45 -0.9521240974783538 1102 46-47 -1.0281176453597212 1102 48-49 -0.8571820147987488 1102 50-51 -0.7163016494806485 1102 52-53 -0.035119644369576264 1102 54-55 0.11950868044059604 1102 56-57 -0.08125541041024675 1102 58-59 0.216429377408069 1102 60-61 0.33118365315038645 1102 62-63 0.18548829461368044 1102 64-65 0.4849394723839957 1102 66-67 0.42381850354125916 1102 68-69 0.022344105672534198 1102 70-71 -0.14546170635034628 1102 72-73 0.016363930829822237 1102 74-75 -0.07924475341507176 1102 76-77 -0.14271835766074048 1102 78-79 0.07755791102071186 1102 80-81 -0.004878544953619723 1102 82-83 0.24781705527538733 1102 84-85 0.2816986805462811 1102 86-87 0.4843728260687321 1102 88-89 0.5631852806182387 1102 90-91 0.4062932705898916 1102 92-93 0.224841639097221 1102 94-95 0.5078311505993902 1102 96-97 0.9633643005113939 1102 98-99 1.1857619657521532 1102 100-101 1.4365126422552024 1103 1 0.09144799580912633 1103 2 0.036049860231983644 1103 3 -0.09710640851069385 1103 4 -0.09648240389608276 1103 5 -0.5388096278435057 1103 6 -0.606732905699495 1103 7 -0.2853789890580529 1103 8 -0.041593645164219595 1103 9 -0.06280349985485145 1103 10-11 -0.15157463995820564 1103 12-13 -0.3689926433329376 1103 14-15 -0.2923287593438815 1103 16-17 -0.6017166006101675 1103 18-19 -0.3786319942661365 1103 20-21 0.1033601974585352 1103 22-23 -0.4177696567267404 1103 24-25 -0.3032797927955997 1103 26-27 0.04924336625599324 1103 28-29 -0.050135406264573135 1103 30-31 0.2825978974931971 1103 32-33 0.4355185784541007 1103 34-35 -0.07637687317675557 1103 36-37 -0.5201552160119505 1103 38-39 -0.2606025194611661 1103 40-41 -1.0069916450852574 1103 42-43 -0.9994570499838886 1103 44-45 -0.740735790489218 1103 46-47 -0.9208523625564453 1103 48-49 -0.9280097936345229 1103 50-51 -1.54619119432925 1103 52-53 -1.1510816833462805 1103 54-55 -0.9407020730666744 1103 56-57 -0.8849435967874442 1103 58-59 -1.3252293542620883 1103 60-61 -1.4219109954879734 1103 62-63 -1.0092682596934317 1103 64-65 -1.6371166439113125 1103 66-67 -1.6567693210000982 1103 68-69 -1.303562070928752 1103 70-71 -0.97216138260249 1103 72-73 -1.5514802893187216 1103 74-75 -1.3673803466354109 1103 76-77 -0.9418677260350172 1103 78-79 -1.3736577304444069 1103 80-81 -0.942461862416959 1103 82-83 -1.1479012206085706 1103 84-85 -1.5284009830098668 1103 86-87 -0.9832999981552284 1103 88-89 -0.59916474794775 1103 90-91 -0.7254594114389263 1103 92-93 -0.48849867703304284 1103 94-95 -0.6353161864157784 1103 96-97 -0.50495919342627 1103 98-99 0.1021905825235585 1103 100-101 -0.512401850222421 1104 1 -0.01480899080678455 1104 2 -0.11105428188711741 1104 3 -0.4039434730745768 1104 4 -0.3400849296521429 1104 5 -0.011301243904412672 1104 6 -0.24439567496744985 1104 7 -0.09171754913539587 1104 8 -0.01570162425724675 1104 9 0.27068089544410157 1104 10-11 0.023838514517755982 1104 12-13 0.273398365458867 1104 14-15 0.21940875538465576 1104 16-17 0.40130463870220723 1104 18-19 0.37545423190383787 1104 20-21 0.3985201720801257 1104 22-23 0.5345249745310028 1104 24-25 0.3732059016362612 1104 26-27 0.3496905096742182 1104 28-29 0.5979657448276043 1104 30-31 0.42710377380365827 1104 32-33 0.19641769927346076 1104 34-35 0.16616821881998334 1104 36-37 0.08312584988127725 1104 38-39 0.5388174025908654 1104 40-41 0.2427288902783502 1104 42-43 0.20032239954630882 1104 44-45 0.33000652800983943 1104 46-47 0.05544601503804358 1104 48-49 -0.8340341448233843 1104 50-51 -1.8044618369468495 1104 52-53 -2.7130473016429058 1104 54-55 -2.4771986439600546 1104 56-57 -2.4862004323413878 1104 58-59 -2.479493833551494 1104 60-61 -2.338977976363527 1104 62-63 -2.398751325647087 1104 64-65 -2.413801740137785 1104 66-67 -1.9164146275048246 1104 68-69 -1.7735460583603953 1104 70-71 -1.7447526995607063 1104 72-73 -2.036706429081253 1104 74-75 -2.1434158764097226 1104 76-77 -2.1478256099590034 1104 78-79 -2.0064714106160118 1104 80-81 -2.025893386027949 1104 82-83 -2.3383903592532427 1104 84-85 -1.9456341320476014 1104 86-87 -1.6626709761303928 1104 88-89 -1.6340147434158894 1104 90-91 -1.9553001921271616 1104 92-93 -1.738521336327885 1104 94-95 -1.7379778982499765 1104 96-97 -1.6754628340196405 1104 98-99 -1.6023171065304886 1104 100-101 -0.45856336567605993 1105 1 -0.013174234219270886 1105 2 0.03701587410745333 1105 3 0.28628400746073623 1105 4 0.21188093441369205 1105 5 0.28932772176570154 1105 6 0.05653807582915249 1105 7 -0.1975888189810604 1105 8 0.0420690110812032 1105 9 0.14123033990393452 1105 10-11 0.025542540452200058 1105 12-13 0.1424238842862806 1105 14-15 0.2631731287741417 1105 16-17 0.4185111444792611 1105 18-19 0.3365110050605793 1105 20-21 0.34539058298589254 1105 22-23 0.00412958509873107 1105 24-25 0.39156227857233716 1105 26-27 0.4911800777105668 1105 28-29 0.5545892797809131 1105 30-31 0.37760389848848064 1105 32-33 0.5296171118693991 1105 34-35 0.46854277203269135 1105 36-37 0.4622369856214945 1105 38-39 0.3439904374194924 1105 40-41 0.36770464600692065 1105 42-43 0.20662591011681286 1105 44-45 0.43525437373315157 1105 46-47 0.5610568321392648 1105 48-49 1.0627545282530022 1105 50-51 1.161194420845952 1105 52-53 1.0601094410353191 1105 54-55 0.9011643828333291 1105 56-57 0.8503100550377347 1105 58-59 1.1651544167048016 1105 60-61 1.1911126279422568 1105 62-63 1.0693155054188281 1105 64-65 1.2339497768609142 1105 66-67 1.2474489395894643 1105 68-69 1.3372710428559742 1105 70-71 0.8798628555638395 1105 72-73 1.032562917847347 1105 74-75 0.8948485108336328 1105 76-77 1.0139737305968133 1105 78-79 0.3390995073413734 1105 80-81 0.5509955890627971 1105 82-83 0.6552368608293264 1105 84-85 0.252240684388628 1105 86-87 0.505986029771357 1105 88-89 0.12556520726433362 1105 90-91 0.1410257617117452 1105 92-93 0.5119843299046956 1105 94-95 0.5754797504713132 1105 96-97 -0.029387635216615138 1105 98-99 0.6048398023941708 1105 100-101 0.8347923185665458 1106 1 -0.17976818022417973 1106 2 -0.2621660184477683 1106 3 -0.1612753053290099 1106 4 -0.09395489869239526 1106 5 -0.1270344271298569 1106 6 -0.04681616469444094 1106 7 -0.09548900987280007 1106 8 -0.2889918015721875 1106 9 0.06809153434080883 1106 10-11 -0.189769703889219 1106 12-13 -0.42996498945247197 1106 14-15 -0.2912582865517379 1106 16-17 -0.9373246886268163 1106 18-19 -0.5087985122560283 1106 20-21 -0.39556932974931414 1106 22-23 -0.4072284732595932 1106 24-25 -0.18720783323568213 1106 26-27 -0.650816104454691 1106 28-29 -0.20432809136716656 1106 30-31 -0.20198432076431772 1106 32-33 -0.3304047043291263 1106 34-35 -0.17236260020619198 1106 36-37 0.0033059793493350753 1106 38-39 0.42539212817487737 1106 40-41 0.53909815568786 1106 42-43 0.5995765509676403 1106 44-45 0.4795139864956255 1106 46-47 0.47540098267102593 1106 48-49 0.5530190496601506 1106 50-51 0.08204252556370051 1106 52-53 -0.13615453496685959 1106 54-55 -0.2626616329834164 1106 56-57 -0.2547622111198855 1106 58-59 0.07423540934183492 1106 60-61 -0.48948459049243453 1106 62-63 -0.3531279088162407 1106 64-65 -0.5690226801884428 1106 66-67 -1.017399710533251 1106 68-69 -0.01331526747945233 1106 70-71 0.12248625343676167 1106 72-73 -0.0173143660359365 1106 74-75 0.1549657729007201 1106 76-77 -0.0353854185714475 1106 78-79 -0.38095776017976135 1106 80-81 -0.5631576697318579 1106 82-83 -0.5179428501060421 1106 84-85 -0.5253977121207818 1106 86-87 -0.4322741283960845 1106 88-89 -1.0061228210967208 1106 90-91 -0.9024293787300337 1106 92-93 -0.17181714268870607 1106 94-95 -0.5577217766625822 1106 96-97 -0.24074569357494724 1106 98-99 -0.25894530805033256 1106 100-101 0.265430442373475 1107 1 -0.3184002935022967 1107 2 -0.05033059231899628 1107 3 -0.06541636864720601 1107 4 -0.07164658984921601 1107 5 7.240553708101061E-4 1107 6 0.030394628786723388 1107 7 -0.22736183915412767 1107 8 -0.1091895039499633 1107 9 -0.0032950539573519677 1107 10-11 -0.07490195075002504 1107 12-13 0.025698916505312752 1107 14-15 0.120817588414873 1107 16-17 0.16036433087625568 1107 18-19 -0.28965611311095785 1107 20-21 -0.33882340402128364 1107 22-23 -0.4474235039836074 1107 24-25 -0.5570582206254713 1107 26-27 -0.5224965746657517 1107 28-29 -0.4923190077443991 1107 30-31 -0.19940617489201884 1107 32-33 -0.24937344598020417 1107 34-35 -0.5423064954773693 1107 36-37 -0.06773329396084193 1107 38-39 -0.6921606369867632 1107 40-41 -0.09889276068937392 1107 42-43 -0.36637322194179234 1107 44-45 -0.6851133178057367 1107 46-47 -0.8161031583922878 1107 48-49 -0.18868546543469478 1107 50-51 0.1843000729855575 1107 52-53 0.24396836110581077 1107 54-55 0.38594910706467545 1107 56-57 0.1664774362218182 1107 58-59 0.12048173581792554 1107 60-61 0.6035557514792487 1107 62-63 0.3985151582422688 1107 64-65 0.43912586376025686 1107 66-67 -0.05579137497354125 1107 68-69 -0.18058750899694687 1107 70-71 0.4164215994159228 1107 72-73 0.7049939428072385 1107 74-75 0.03243037348848432 1107 76-77 -0.1831278711495905 1107 78-79 -0.265395377064614 1107 80-81 -0.542479069678425 1107 82-83 -0.4917740015888086 1107 84-85 -0.19106696138607404 1107 86-87 -0.3314459157324414 1107 88-89 -0.1678647393441146 1107 90-91 -0.1973398783292879 1107 92-93 0.14851414237675087 1107 94-95 -0.12970948705531882 1107 96-97 0.2347953163101728 1107 98-99 0.4449018733549579 1107 100-101 1.528895577291987 1108 1 -0.3616952200459558 1108 2 -0.36929495189249195 1108 3 0.012659531763723919 1108 4 -0.23333111701597886 1108 5 -0.4363076100872476 1108 6 -0.2707092306927663 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2316 96-97 -3.878932828077872 2316 98-99 -4.135436536120508 2316 100-101 -4.641177744761027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 6.0 5 31.0 6 66.0 7 145.0 8 276.0 9 475.0 10 831.0 11 1232.0 12 1728.0 13 2366.0 14 2620.0 15 3409.0 16 4241.0 17 5592.0 18 5211.0 19 3073.0 20 2543.0 21 2686.0 22 2749.0 23 2902.0 24 3126.0 25 3331.0 26 3199.0 27 3244.0 28 3876.0 29 4488.0 30 4960.0 31 5849.0 32 6904.0 33 8455.0 34 11248.0 35 16050.0 36 19411.0 37 28262.0 38 26414.0 39 3245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99924556381187 22.102396626740113 16.414671200173625 18.483686609274397 2 15.502507130133955 23.98556469631292 40.04870113155483 20.46322704199829 3 18.2943278111261 30.46240334421301 33.47559280499984 17.767676039661048 4 11.659442150674918 21.266332382648805 36.74001009029787 30.33421537637841 5 10.473317339868002 40.58822318091492 33.80970521915509 15.128754260061983 6 24.812866159406113 39.31818415823234 18.302564788978923 17.566384893382615 7 21.544330385181677 38.975319955108475 21.60559290796207 17.874756751747782 8 24.65790801354983 33.98937429856985 21.486156729096095 19.86656095878422 9 25.896028747052707 17.209105979016297 21.878442799336923 35.01642247459407 10-11 22.918876064371982 29.251310194289715 26.74315043810426 21.086663303234044 12-13 25.812371940734945 28.08989631704128 27.642010646293873 18.455721095929903 14-15 22.725108174964156 28.12857955525353 29.191152433389707 19.955159836392607 16-17 18.165624573659972 31.768091618380307 29.6851068649432 20.38117694301652 18-19 17.646438021889768 28.14549591754785 35.481811723278724 18.726254337283653 20-21 19.79886329705631 26.661552876249704 35.63960133027192 17.899982496422062 22-23 19.05779269585989 28.064670572367 35.380136527907915 17.4974002038652 24-25 18.530626113279038 28.835342812722015 35.706784180884036 16.927246893114916 26-27 17.976431947118602 28.964045591672416 36.304222480771806 16.755299980437176 28-29 17.610143838225756 28.961214130535506 36.11348496236731 17.315157068871432 30-31 18.6781194979562 28.69196791697126 35.719654458779075 16.910258126293463 32-33 17.677326688837866 29.162247871256035 35.938963994110566 17.221461445795537 34-35 17.71429159823511 28.377258241390496 36.23586105347701 17.672589106897384 36-37 17.52066668897888 29.078565599198818 35.725631843306466 17.675135868515834 38-39 17.52331035334943 28.14207087583086 35.944539121749294 18.39007964907042 40-41 17.689759292008063 27.976544420676536 36.23027242141789 18.103423865897515 42-43 17.808289701968175 28.453570564401954 35.41605531273006 18.32208442089981 44-45 17.27605024711697 28.99814662273476 34.84117586490939 18.88462726523888 46-47 18.500423441952414 28.624161160205208 33.27841887547203 19.59699652237035 48-49 18.086344120342247 28.85258898510137 32.9525447113454 20.10852218321098 50-51 17.70496023063657 29.587891580220855 32.571494761769934 20.13565342737265 52-53 17.835888512504763 29.9159313447896 32.70414834797113 19.54403179473451 54-55 17.52520000411849 29.711551331816356 31.914428096331456 20.848820567733696 56-57 18.466789534919638 30.069087651740578 30.633578040217046 20.830544773122742 58-59 17.639230666268546 30.626885495711626 31.567960215396973 20.16592362262286 60-61 18.1673097565182 31.437698364689865 30.29583849220956 20.099153386582376 62-63 17.48066884260165 31.982640569175167 30.37385583229513 20.16283475592805 64-65 17.488391009338674 31.934505729847718 30.127003902268257 20.450099358545348 66-67 17.634309513574458 31.430395354284702 29.873586503538085 21.06170862860276 68-69 17.67214861758566 31.263851814861244 29.773969270529 21.290030297024096 70-71 18.148846021179875 30.799882620044173 29.40367893493135 21.647592423844607 72-73 17.95609690804444 30.374628048968834 29.555820969286366 22.11345407370036 74-75 17.39443798070488 30.768458552557064 29.097896481780833 22.73920698495722 76-77 17.325058032735356 30.36612794768938 29.82766660741016 22.481147412165104 78-79 17.298001581019527 30.110954611349865 29.676302989259884 22.914740818370728 80-81 17.073195844444673 30.247727108923733 29.43149408482028 23.24758296181131 82-83 17.18362282878412 30.012201023444497 29.315146772649115 23.489029375122268 84-85 17.282209157460127 29.643853670088443 29.08399658165419 23.989940590797236 86-87 17.266863954135818 29.71608133468066 28.76770947186076 24.24934523932276 88-89 17.364190150574174 29.475972947778057 28.741823778376297 24.41801312327147 90-91 17.39935155451382 29.155007382779026 28.85479157846913 24.590849484238024 92-93 17.37796402499923 29.322096722712438 28.63482388311728 24.66511536917105 94-95 17.185211497821058 29.048291981024015 28.75510629589875 25.011390225256186 96-97 17.055692266507418 28.907416368934236 28.817324423668957 25.219566940889386 98-99 17.205076063733944 28.743597003783883 28.64449535380576 25.406831578676414 100-101 17.448370481532297 28.497589378335036 29.034798926095718 25.019241214036946 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1832.0 1 1192.5 2 587.5 3 571.0 4 442.0 5 437.5 6 658.0 7 1065.5 8 1427.0 9 1505.5 10 1235.0 11 934.5 12 881.5 13 919.5 14 925.0 15 907.0 16 948.5 17 979.5 18 1026.0 19 1096.5 20 1144.5 21 1235.5 22 1403.5 23 1551.5 24 1743.0 25 1992.0 26 2370.0 27 2871.0 28 3055.5 29 3172.5 30 3530.0 31 3825.5 32 4208.5 33 4658.5 34 4948.0 35 5139.0 36 5417.0 37 5648.5 38 5493.5 39 5412.0 40 5661.0 41 5799.0 42 5755.0 43 5891.0 44 6123.0 45 6153.0 46 6099.5 47 6278.5 48 6589.5 49 6390.5 50 5918.5 51 5245.5 52 4229.5 53 3503.0 54 3139.0 55 2859.0 56 2531.5 57 2234.0 58 2066.0 59 1962.5 60 1778.0 61 1617.5 62 1394.0 63 1042.5 64 809.0 65 723.0 66 672.5 67 607.5 68 572.5 69 504.0 70 427.0 71 412.5 72 386.0 73 324.5 74 272.5 75 213.5 76 166.0 77 118.5 78 91.5 79 76.5 80 42.5 81 28.0 82 23.0 83 14.0 84 9.0 85 5.0 86 4.5 87 4.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37272324784036737 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.574055579008062E-4 16-17 0.0028314611369088683 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00669254450542096 36-37 0.016731361263552402 38-39 0.009781411200230634 40-41 0.00334627225271048 42-43 0.002574055579008062 44-45 0.0030888666948096746 46-47 0.0023166500211072557 48-49 0.0 50-51 5.148111158016124E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 7.722166737024187E-4 62-63 0.0 64-65 0.0 66-67 0.001287027789504031 68-69 0.001287027789504031 70-71 0.002574055579008062 72-73 0.0 74-75 0.0 76-77 0.08983453970738137 78-79 0.03474975031660884 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14517673465605468 88-89 0.3194402973549005 90-91 0.28417573592249007 92-93 0.0 94-95 2.574055579008062E-4 96-97 0.0 98-99 5.148111158016124E-4 100-101 7.722166737024187E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 194246.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.5446899496247 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81515031238942 45.35393388059516 2 13.10209130200262 15.079115633308305 3 3.7634964428174267 6.497077077853327 4 1.6216520914472101 3.732698672339605 5 0.7491566156816694 2.15549925865559 6 0.4565484378047546 1.5763162980276069 7 0.2655614669602551 1.0697156595156767 8 0.2108558452297897 0.9706907390251438 9 0.15515779234148613 0.8035656348203165 >10 0.7598750136495669 7.652757404684009 >50 0.0606706481210423 2.4301553860531153 >100 0.029838023666086382 3.162750509498237 >500 0.002983802366608638 1.0353629518064262 >1k 0.0069622055220868225 8.480360893817478 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3294 1.6957878154505113 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 1.4203638684966486 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2281 1.174284155143478 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2180 1.122288232447515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1834 0.9441635863801571 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1313 0.6759469950475171 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1156 0.5951216498666639 RNA PCR Primer, Index 33 (95% over 22bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 633 0.32587543630242066 RNA PCR Primer, Index 33 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 631 0.3248458140708174 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.28057205811187874 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 409 0.21055774636285945 RNA PCR Primer, Index 33 (96% over 29bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 401 0.20643925743644656 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 388 0.19974671293102564 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 314 0.1616506903617063 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 297 0.15289890139307888 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 258 0.132821267876816 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 231 0.11892136775017245 RNA PCR Primer, Index 33 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 209 0.10759552320253699 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.148111158016124E-4 0.0 18-19 0.0 0.0 0.0 5.148111158016124E-4 0.0 20-21 0.0 0.0 0.0 0.001287027789504031 0.0 22-23 0.0 0.0 0.0 0.004375894484313705 0.0 24-25 0.0 0.0 0.0 0.012355466779238697 0.0 26-27 0.0 0.0 0.0 0.03166088362179916 0.0 28-29 0.0 0.0 0.0 0.07541982846493622 0.0 30-31 0.0 0.0 0.0 0.17194691267773854 0.0 32-33 0.0 0.0 0.0 0.34363641979757625 0.0 34-35 0.0 0.0 0.0 0.5848254275506317 0.0 36-37 0.0 0.0 0.0 0.9029786971160281 0.0 38-39 0.0 0.0 2.574055579008062E-4 1.320233106473235 0.0 40-41 0.0 0.0 5.148111158016124E-4 1.7997796608424368 0.0 42-43 0.0 0.0 5.148111158016124E-4 2.1900064866200593 0.0 44-45 0.0 0.0 5.148111158016124E-4 2.638921779599065 0.0 46-47 0.0 0.0 5.148111158016124E-4 3.1290219618422004 0.0 48-49 0.0 0.0 5.148111158016124E-4 3.6386849664857963 0.0 50-51 0.0 0.0 5.148111158016124E-4 4.200086488267455 0.0 52-53 0.0 0.0 5.148111158016124E-4 4.685553370468375 0.0 54-55 0.0 0.0 5.148111158016124E-4 5.2299661254285805 0.0 56-57 0.0 0.0 5.148111158016124E-4 5.860609742285556 0.0 58-59 0.0 0.0 5.148111158016124E-4 6.4814719479423 0.0 60-61 0.0 0.0 5.148111158016124E-4 7.176724359832377 0.0 62-63 0.0 0.0 5.148111158016124E-4 7.845721404816572 0.0 64-65 0.0 0.0 5.148111158016124E-4 8.532994244411725 0.0 66-67 0.0 0.0 5.148111158016124E-4 9.28152960678727 0.0 68-69 0.0 0.0 5.148111158016124E-4 10.119642103312295 0.0 70-71 0.0 0.0 5.148111158016124E-4 11.010265333649084 0.0 72-73 0.0 0.0 5.148111158016124E-4 11.864851785879761 0.0 74-75 0.0 0.0 5.148111158016124E-4 12.722527104805247 0.0 76-77 0.0 0.0 5.148111158016124E-4 13.604398546173408 0.0 78-79 0.0 0.0 5.148111158016124E-4 14.550878782574674 0.0 80-81 0.0 0.0 5.148111158016124E-4 15.555017863945718 0.0 82-83 0.0 0.0 5.148111158016124E-4 16.576403117696117 0.0 84-85 0.0 0.0 5.148111158016124E-4 17.57693852125655 0.0 86-87 0.0 0.0 5.148111158016124E-4 18.57541468035378 0.0 88-89 0.0 0.0 5.148111158016124E-4 19.589335172925054 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1975 0.0 44.933598 1 GTATTGG 70 4.162368E-6 40.89579 1 TATAGAC 60 8.312852E-5 39.54482 2 GTACAGG 50 0.0015776347 38.169403 1 ACCGAGG 35 2.0594809E-4 33.90429 78-79 ATAAGCC 75 3.086677E-4 31.635857 7 GTATCAA 2900 0.0 30.930378 1 TGGGATA 140 1.7065759E-8 30.506004 6 ATTAGTG 65 0.0058600837 29.202328 9 ATGGGGC 100 4.9650647E-5 28.47227 5 CATGGGG 450 0.0 27.417744 4 ATAGAGT 105 6.9225345E-5 27.11645 6 GTACATG 1930 0.0 25.709959 1 ATGGGAT 470 0.0 25.241373 5 TATCAAC 3595 0.0 25.211885 2 TACATGG 1940 0.0 24.949928 2 CAACGCA 3685 0.0 24.467352 5 TCAACGC 3705 0.0 24.335274 4 ATCAACG 3710 0.0 24.302477 3 ACATGGG 1905 0.0 23.91372 3 >>END_MODULE