FastQCFastQC Report
Thu 26 May 2016
SRR938864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193545
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34171.7654808959156787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26381.3629905190007492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21931.1330698287220025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18330.9470665736650391No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.7130124777183601No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.29708853238265004No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.2722880983750549No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.2604045570797489No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3850.19892014776925265No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.16998630809372497No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.15500271254746958No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2380.12296881862099253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.034.874671
GTATCAA29600.031.9574151
ACTTGGC600.00396892931.6241058
GGGTAAC650.005811417529.2520921
GGAGTGC650.005870647329.191488
CACCAGA1004.9075723E-528.5207881
ACAGTGC908.990819E-426.353428
TAGAATG908.990819E-426.353425
TATCAAC35650.026.346032
TGGGATC1658.443749E-825.8742686
TCAACGC36250.025.5173824
ATCAACG36450.025.507513
CAACGCA36350.025.3166835
AACGCAG36800.025.1360076
CTTGGCT950.001232485424.9663989
GTTAGTA601.7115219E-423.74267626-27
ATATGTA1000.001661640523.7180795
GAGTACT23400.022.92501812-13
TGGGATA1050.002206837522.5886466
ACGCAGA41000.022.5610987
Produced by FastQC (version 0.11.5)