##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938864_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193545 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93211914541838 31.0 31.0 34.0 28.0 34.0 2 30.923562995685757 33.0 31.0 34.0 27.0 34.0 3 30.844051770905992 33.0 31.0 34.0 27.0 34.0 4 34.42629362680513 37.0 35.0 37.0 32.0 37.0 5 34.462228422330725 37.0 35.0 37.0 32.0 37.0 6 34.54766591748689 37.0 35.0 37.0 32.0 37.0 7 34.47827636983647 37.0 35.0 37.0 32.0 37.0 8 34.45869436048464 37.0 35.0 37.0 31.0 37.0 9 35.907458213852074 39.0 37.0 39.0 31.0 39.0 10-11 35.80483608463148 39.0 37.0 39.0 30.0 39.0 12-13 35.667979022966236 39.0 35.0 39.0 30.0 39.0 14-15 36.61290655919812 40.0 36.5 41.0 30.0 41.0 16-17 36.50272804774083 39.0 36.5 41.0 30.0 41.0 18-19 36.532930326280706 40.0 36.0 41.0 30.0 41.0 20-21 36.47960164302876 40.0 36.0 41.0 30.0 41.0 22-23 36.33192539202769 40.0 36.0 41.0 30.0 41.0 24-25 36.190103076803844 40.0 35.0 41.0 29.0 41.0 26-27 35.964917719393426 39.0 35.0 41.0 28.0 41.0 28-29 35.69389805988271 39.0 35.0 41.0 27.0 41.0 30-31 35.232054560954815 38.5 35.0 40.5 26.5 41.0 32-33 34.74278333204164 38.0 35.0 40.0 24.5 41.0 34-35 34.26171433000077 38.0 34.5 40.0 23.0 41.0 36-37 33.8948461598078 38.0 34.0 40.0 20.0 41.0 38-39 33.53813066728668 37.5 33.5 40.0 17.0 41.0 40-41 33.15173473869126 37.0 33.0 40.0 12.0 41.0 42-43 32.759223953085844 37.0 33.0 40.0 4.5 41.0 44-45 32.211671704254826 37.0 33.0 40.0 2.0 41.0 46-47 31.431524968353614 37.0 31.0 40.0 2.0 41.0 48-49 30.744475444986954 37.0 30.0 40.0 2.0 41.0 50-51 29.49263995453254 36.0 27.0 39.0 2.0 40.0 52-53 29.473269782221188 35.5 27.0 39.0 2.0 40.0 54-55 29.814084579813482 36.0 28.0 40.0 2.0 41.0 56-57 29.635927045389963 35.5 27.0 40.0 2.0 41.0 58-59 29.383647213826244 35.0 26.5 40.0 2.0 41.0 60-61 29.026396961946833 35.0 26.0 40.0 2.0 41.0 62-63 28.62111911958459 35.0 24.0 39.0 2.0 41.0 64-65 28.211335865044305 35.0 22.5 39.0 2.0 41.0 66-67 27.801268438864348 35.0 21.0 39.0 2.0 40.5 68-69 27.34039370688987 35.0 19.5 38.0 2.0 40.0 70-71 26.890327830737036 34.5 15.0 37.5 2.0 40.0 72-73 26.413454235449123 34.0 7.5 37.0 2.0 39.5 74-75 25.902697047198323 34.0 2.0 36.0 2.0 39.0 76-77 25.39087809036658 34.0 2.0 36.0 2.0 39.0 78-79 24.890648169676304 33.0 2.0 35.0 2.0 37.5 80-81 24.3279159885298 33.0 2.0 35.0 2.0 37.0 82-83 23.818233485752668 33.0 2.0 35.0 2.0 37.0 84-85 23.399532408483815 32.5 2.0 35.0 2.0 36.0 86-87 22.857989098142554 32.0 2.0 35.0 2.0 36.0 88-89 22.30982717197551 31.5 2.0 35.0 2.0 35.0 90-91 21.772042677413523 31.0 2.0 35.0 2.0 35.0 92-93 21.215740525459196 30.5 2.0 35.0 2.0 35.0 94-95 20.629706269859724 30.0 2.0 34.5 2.0 35.0 96-97 19.99639360355473 29.0 2.0 34.0 2.0 35.0 98-99 19.30864915136015 28.0 2.0 34.0 2.0 35.0 100-101 17.460293471802423 21.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03253010937583056 1101 2 0.13142109998500828 1101 3 0.06744985056684882 1101 4 -0.0996527712419848 1101 5 -0.03201413137459497 1101 6 -0.20982560349749946 1101 7 -0.11228182282088284 1101 8 -0.41428873118243814 1101 9 -0.18011247723200086 1101 10-11 -0.30931631999099096 1101 12-13 -0.38163157810976855 1101 14-15 -0.11696755336207332 1101 16-17 -0.34464607581935525 1101 18-19 0.11998498694669735 1101 20-21 0.06368292444782497 1101 22-23 0.20912639952266687 1101 24-25 -0.05412831013416053 1101 26-27 -0.6683329199038823 1101 28-29 -0.9101793658226001 1101 30-31 -0.722177419774475 1101 32-33 -0.8988679052288333 1101 34-35 -0.5362320398162836 1101 36-37 -0.1520640293721982 1101 38-39 -0.14383266495025993 1101 40-41 -1.1449956002741644 1101 42-43 -1.1745242948838097 1101 44-45 -0.5292909469945322 1101 46-47 -0.5106133226397702 1101 48-49 -0.5779001954719902 1101 50-51 -0.624415519198358 1101 52-53 -0.3106456231251613 1101 54-55 -0.20258359663388603 1101 56-57 -0.32384170408292334 1101 58-59 -0.7859131110881599 1101 60-61 -1.0452859943986859 1101 62-63 -1.102001646722826 1101 64-65 -1.1100136742829463 1101 66-67 -1.1305443243656832 1101 68-69 -0.9770833387697699 1101 70-71 -1.4095879905552025 1101 72-73 -1.2657417546843064 1101 74-75 -1.3566188106867862 1101 76-77 -1.511216610687292 1101 78-79 -1.6661209864657351 1101 80-81 -1.3557271826497654 1101 82-83 -1.2243319392695575 1101 84-85 -1.102599690236687 1101 86-87 -1.705291634077554 1101 88-89 -1.5158414564366431 1101 90-91 -1.8691718448073438 1101 92-93 -1.3285298632308589 1101 94-95 -1.192835093627668 1101 96-97 -1.5909407789400625 1101 98-99 -1.7101820889896757 1101 100-101 -1.108713643045192 1102 1 -0.11492868072467743 1102 2 -0.009351264544260829 1102 3 -0.04887918565169258 1102 4 -0.08916111434148632 1102 5 -0.2298767353145479 1102 6 -0.06453289743131307 1102 7 -0.11836475155574533 1102 8 0.046981428982753926 1102 9 0.29547209780008643 1102 10-11 0.23032037848549436 1102 12-13 -0.01281914438020948 1102 14-15 -0.09050123269685173 1102 16-17 -0.08303828520604384 1102 18-19 -0.08691830006709012 1102 20-21 0.29275541733247223 1102 22-23 0.07679479619658736 1102 24-25 0.0508161622136285 1102 26-27 0.5595257775680764 1102 28-29 0.6858639401654614 1102 30-31 0.33357345918081904 1102 32-33 0.3084678835986665 1102 34-35 0.5519146201535818 1102 36-37 0.11611499847533935 1102 38-39 0.013709608614114188 1102 40-41 -0.21458653726842414 1102 42-43 0.055427153299682175 1102 44-45 0.39768600484430294 1102 46-47 -0.3083001355199535 1102 48-49 -0.2629970200715732 1102 50-51 -0.20095438855490855 1102 52-53 -0.037178911813143145 1102 54-55 0.17206063574515085 1102 56-57 0.33371452115286004 1102 58-59 0.3643463900000725 1102 60-61 0.23849100208549245 1102 62-63 -0.15168354861467037 1102 64-65 0.17118226299033878 1102 66-67 0.08167454945540342 1102 68-69 -0.3287631200075616 1102 70-71 -0.06171701561240184 1102 72-73 -0.11658443563748833 1102 74-75 -0.23343950380581902 1102 76-77 -0.41767065388166813 1102 78-79 -0.5441136199832322 1102 80-81 -0.7778768561806544 1102 82-83 -0.7788271677979353 1102 84-85 -1.0023708828255593 1102 86-87 -1.5210179580911714 1102 88-89 -1.1294220133873658 1102 90-91 -1.5753217415649772 1102 92-93 -2.031847570692211 1102 94-95 -1.6412139094097995 1102 96-97 -1.5392498363651406 1102 98-99 -1.4287768766452373 1102 100-101 -1.5532417975180977 1103 1 0.16241938557366353 1103 2 0.09732336588727364 1103 3 0.10919159230859066 1103 4 0.2789440073547951 1103 5 0.1951493957889312 1103 6 0.27980038612849256 1103 7 0.43388252334346333 1103 8 0.396003379109672 1103 9 0.3105236134040865 1103 10-11 -0.05915251982718672 1103 12-13 0.04024939377119807 1103 14-15 0.6080802216857037 1103 16-17 0.518649037475754 1103 18-19 0.33879470575641335 1103 20-21 -0.07272166195675567 1103 22-23 -0.24313950274323304 1103 24-25 0.030747474741623648 1103 26-27 -0.201830053401018 1103 28-29 -0.19857685422008586 1103 30-31 0.12303142543436962 1103 32-33 0.0775585211975951 1103 34-35 -0.4209986245828645 1103 36-37 -0.21215138945955658 1103 38-39 0.066404845287245 1103 40-41 0.17034701506844385 1103 42-43 -0.11424583460534876 1103 44-45 -0.9417943594979477 1103 46-47 -1.4862753483017954 1103 48-49 -1.7408677084395023 1103 50-51 -1.0750024697853071 1103 52-53 -1.311450973930512 1103 54-55 -1.079201123251412 1103 56-57 -1.1776637079049266 1103 58-59 -1.2906633158383656 1103 60-61 -1.121207370320061 1103 62-63 -1.2698558645770461 1103 64-65 -1.334924949194221 1103 66-67 -1.2100829539043083 1103 68-69 -1.5694529811394133 1103 70-71 -1.1127547937220044 1103 72-73 -1.7611417000842522 1103 74-75 -1.2705008745688495 1103 76-77 -1.57890702837771 1103 78-79 -1.1854903558351069 1103 80-81 -0.9861940131165952 1103 82-83 -0.8457078606454793 1103 84-85 -1.2277703154073123 1103 86-87 -1.1411911699770876 1103 88-89 -0.5965266871218731 1103 90-91 -0.5933596689951663 1103 92-93 -0.8405828252838212 1103 94-95 -0.5400641408567175 1103 96-97 -0.3557646937639767 1103 98-99 0.047257168449583276 1103 100-101 -0.1093415436730929 1104 1 -0.19302102893410833 1104 2 -0.3968931304017609 1104 3 -0.38188808840839883 1104 4 -0.40367747423042033 1104 5 -0.007651332400399724 1104 6 -0.1261651649147808 1104 7 -0.0432495309906642 1104 8 0.15427252908867217 1104 9 -0.350364217431931 1104 10-11 -0.042516194382564265 1104 12-13 -0.062285265240141996 1104 14-15 -0.35105715403197735 1104 16-17 0.038394171210526906 1104 18-19 -0.3704897081912151 1104 20-21 -0.3229947329552161 1104 22-23 -0.3976276696666403 1104 24-25 -0.7711430167861764 1104 26-27 -0.32157518713578526 1104 28-29 -0.22535181571007 1104 30-31 -0.1708349798310067 1104 32-33 -0.33741137097436535 1104 34-35 -0.2147530007206626 1104 36-37 0.06850958239312632 1104 38-39 0.587522335834791 1104 40-41 0.13068870390754483 1104 42-43 0.7022224471479106 1104 44-45 0.3411492058290406 1104 46-47 0.7032219209882236 1104 48-49 0.26177897969941455 1104 50-51 0.7726379167151798 1104 52-53 1.0886190442742247 1104 54-55 1.0635099816358569 1104 56-57 0.99011391427684 1104 58-59 1.2113392048170049 1104 60-61 1.05386614879491 1104 62-63 1.264971190457267 1104 64-65 0.969682248677227 1104 66-67 1.211594682281099 1104 68-69 1.3081131860640625 1104 70-71 1.1542106172848534 1104 72-73 1.423678353129585 1104 74-75 1.58744619166837 1104 76-77 1.7197066111685722 1104 78-79 1.5674714142251105 1104 80-81 1.2007966044439726 1104 82-83 0.7723708398166416 1104 84-85 0.6810659820300238 1104 86-87 0.8613854999570911 1104 88-89 0.724240712402004 1104 90-91 0.7355018349958691 1104 92-93 -0.10878055669398634 1104 94-95 0.020123507931970153 1104 96-97 -0.5452899703358831 1104 98-99 -0.44588104748655866 1104 100-101 0.43569416561132 1105 1 -0.24284377232107346 1105 2 -0.3914390807699064 1105 3 -0.12494781025186796 1105 4 0.130437606216816 1105 5 0.18021340716872913 1105 6 0.17667094089378566 1105 7 0.16517697569054945 1105 8 0.35503609603713215 1105 9 0.21265732606411802 1105 10-11 0.21588304205048559 1105 12-13 0.04949548196465514 1105 14-15 -0.13110896750348644 1105 16-17 0.07263196777447689 1105 18-19 -0.197691780730068 1105 20-21 0.14207207915276854 1105 22-23 0.23246504917710809 1105 24-25 0.30684989294930176 1105 26-27 0.17839754261605378 1105 28-29 0.08442456825501665 1105 30-31 0.3512675563020764 1105 32-33 0.4999625041278932 1105 34-35 0.5178349458296552 1105 36-37 0.6285313985916474 1105 38-39 0.8811274626414445 1105 40-41 1.042537626732745 1105 42-43 1.2777883047972125 1105 44-45 1.2006665224366202 1105 46-47 0.8669252206882483 1105 48-49 0.8131152218592206 1105 50-51 0.7440586966442773 1105 52-53 0.7619487415744715 1105 54-55 0.8183095453118874 1105 56-57 0.5706293219670506 1105 58-59 0.8227790739198113 1105 60-61 0.3905991730654286 1105 62-63 0.5802152591782885 1105 64-65 0.4242500152439028 1105 66-67 0.383942650647608 1105 68-69 0.1616992236970063 1105 70-71 0.19883837839748253 1105 72-73 0.16737892580479397 1105 74-75 0.46036683524622646 1105 76-77 0.351462815150029 1105 78-79 0.6119491889728614 1105 80-81 0.554559898689952 1105 82-83 0.8818881564242211 1105 84-85 0.2873790444788895 1105 86-87 -0.0974500604465085 1105 88-89 0.11172366849690363 1105 90-91 0.6456872726833502 1105 92-93 1.4549363781301174 1105 94-95 0.9685418335387332 1105 96-97 1.1653590615703031 1105 98-99 1.498324024779599 1105 100-101 1.4850396796554968 1106 1 0.21375628396938495 1106 2 -0.059606826337034846 1106 3 0.09745579233749524 1106 4 0.30201092453935985 1106 5 -0.2783005247198105 1106 6 -0.3075677306513924 1106 7 -0.004735774098364232 1106 8 0.08095785229944141 1106 9 -0.17417070658434852 1106 10-11 -0.39710598131005526 1106 12-13 -0.16134043134803733 1106 14-15 -0.14207153434840336 1106 16-17 0.8325672337469001 1106 18-19 0.5956237272913185 1106 20-21 0.18890574084711176 1106 22-23 0.01913828306396681 1106 24-25 0.5878800083447473 1106 26-27 0.3281020177075291 1106 28-29 -0.007624404444108279 1106 30-31 0.23236803477097823 1106 32-33 0.3160756479500151 1106 34-35 0.5707198318414726 1106 36-37 0.7198908131708777 1106 38-39 0.7001958555488486 1106 40-41 0.4729485470817423 1106 42-43 1.0135327461144144 1106 44-45 0.9407031112348179 1106 46-47 1.100052155672767 1106 48-49 0.756472947724685 1106 50-51 0.5915678438438299 1106 52-53 0.2697168248843482 1106 54-55 -0.0951860921775598 1106 56-57 0.30346073204304247 1106 58-59 0.5126608639564054 1106 60-61 0.7675482302002443 1106 62-63 0.4911356081791354 1106 64-65 0.04580926095175286 1106 66-67 0.31315739874780846 1106 68-69 0.2806409630721802 1106 70-71 0.4267162281019594 1106 72-73 0.17558326017011794 1106 74-75 0.0377662160511818 1106 76-77 0.241457186104153 1106 78-79 0.14983266772321358 1106 80-81 0.07742789756414226 1106 82-83 0.3050798254363265 1106 84-85 0.5254454672202371 1106 86-87 0.7231100296182298 1106 88-89 1.383296986819449 1106 90-91 0.8711727778902194 1106 92-93 0.9057244445528632 1106 94-95 0.9286741161168344 1106 96-97 0.46832838327027915 1106 98-99 0.4567816662093733 1106 100-101 0.4477331781075087 1107 1 0.2681188674389503 1107 2 0.4010343307381383 1107 3 -0.05028187079181734 1107 4 0.08855050504419637 1107 5 0.4431258696721372 1107 6 0.12253949829765531 1107 7 0.4023463239316172 1107 8 0.32525698504721845 1107 9 0.07450624888931401 1107 10-11 -0.07619495085925365 1107 12-13 -0.1270521046183788 1107 14-15 -0.042766998591567074 1107 16-17 -0.07818613967030075 1107 18-19 0.11396625035801833 1107 20-21 0.4023938680810488 1107 22-23 0.2629940919913949 1107 24-25 0.1511367759599409 1107 26-27 0.5722071962839053 1107 28-29 0.3135082879168749 1107 30-31 0.4664707196508644 1107 32-33 0.2765782663313274 1107 34-35 -0.38832813025433666 1107 36-37 -0.06879611708873767 1107 38-39 -0.7648092703828269 1107 40-41 -0.7527309852116204 1107 42-43 -0.6552905062199699 1107 44-45 -0.4438248874352091 1107 46-47 -0.8744982862656663 1107 48-49 -1.217495631699549 1107 50-51 -0.42684394856979324 1107 52-53 -0.35697681064805664 1107 54-55 0.05314728733533869 1107 56-57 0.016020650028135464 1107 58-59 -0.15653644285139023 1107 60-61 -0.11711830716363991 1107 62-63 -0.0407282914080227 1107 64-65 -0.105447285001528 1107 66-67 0.04579157347280827 1107 68-69 0.1112219943542172 1107 70-71 0.5246769385283798 1107 72-73 0.02471462255963175 1107 74-75 -0.16643981869432167 1107 76-77 0.06944754381106222 1107 78-79 0.34726416033909757 1107 80-81 -0.1878044318254588 1107 82-83 -0.04369133634916267 1107 84-85 0.2484234949833919 1107 86-87 0.13340622534569135 1107 88-89 -0.09182146376380018 1107 90-91 -0.2023219992010219 1107 92-93 -0.5828738209426909 1107 94-95 -0.43842571486595716 1107 96-97 -0.5530619910612735 1107 98-99 0.1292484861921963 1107 100-101 0.6766268080983657 1108 1 0.6272545504088285 1108 2 0.4518229203868316 1108 3 0.1822239653409632 1108 4 0.17196450037528876 1108 5 0.01549391613345108 1108 6 -0.10065699432888664 1108 7 0.4065881960491353 1108 8 0.032653215759509635 1108 9 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-1.970677390949234 2316 64-65 -2.001694802748215 2316 66-67 -1.50186769018244 2316 68-69 -1.7795017964745696 2316 70-71 -1.6413772487761804 2316 72-73 -1.2368292819392366 2316 74-75 -1.0345323068822196 2316 76-77 -0.8252935029580151 2316 78-79 -0.7127436002689684 2316 80-81 -0.39217091512873736 2316 82-83 0.07562801267277308 2316 84-85 -0.2679536705686374 2316 86-87 -0.39400698138321033 2316 88-89 -0.16142727052465844 2316 90-91 0.059587110383329644 2316 92-93 0.20016012030493968 2316 94-95 0.4719849126020286 2316 96-97 0.6980393305246295 2316 98-99 0.34991224643505703 2316 100-101 -0.1814453655566517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3869.0 3 713.0 4 542.0 5 560.0 6 578.0 7 711.0 8 814.0 9 880.0 10 1114.0 11 1561.0 12 2050.0 13 2648.0 14 2734.0 15 3483.0 16 4237.0 17 5219.0 18 4444.0 19 2749.0 20 2536.0 21 2450.0 22 2512.0 23 2714.0 24 2740.0 25 3054.0 26 3324.0 27 3842.0 28 4221.0 29 4687.0 30 5384.0 31 5911.0 32 7140.0 33 8518.0 34 10724.0 35 15456.0 36 17851.0 37 23985.0 38 23164.0 39 4426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72713332504455 20.72454308093995 15.851775871358118 17.69654772265738 2 15.760900504593092 23.34532281019537 40.26031828179583 20.63345840341571 3 19.14423276040588 30.117001449575483 32.17073928349555 18.56802650652309 4 11.947667498162772 19.300715224659207 38.13513709335183 30.616480183826194 5 11.032789687122888 39.72777982069516 33.43760844006857 15.801822052113385 6 25.667148100407406 38.1217872062866 19.315846417459998 16.895218275846002 7 22.56375463528827 38.355327215098086 22.547699446872862 16.533218702740776 8 25.130391095595968 32.69333360956737 21.563346506243754 20.612928788592917 9 27.028201811125484 17.06856403622251 22.732729624838292 33.17050452781371 10-11 23.11943895765 28.925057147516963 27.00754114232903 20.947962752504008 12-13 26.0630820252732 28.183420968831797 27.904074727061374 17.849422278833632 14-15 23.03296930800683 27.736918378965893 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40-41 18.064322134290016 28.326986834362856 35.39817044562014 18.21052058572699 42-43 17.641323514158284 28.872523597137228 34.751841095322064 18.73431179338243 44-45 17.950513357140448 28.7627820611568 34.34396873435875 18.942735847344004 46-47 18.21147910885183 28.987473091786185 33.669631973441916 19.13141582592007 48-49 18.198796915247115 29.230445981805875 32.95072229182717 19.620034811119844 50-51 18.37031657307378 29.781731879077128 32.25595600918204 19.59199553866705 52-53 18.297737683317163 29.909683711423636 31.77047938703201 20.022099218227186 54-55 18.066070645844842 29.62726652609082 31.991638071672927 20.315024756391402 56-57 18.459424066798917 29.68454626784395 31.397305742476693 20.458723922880445 58-59 18.59751276155021 29.527206925069155 31.406764853044493 20.468515460336143 60-61 18.476728516637298 29.390484088317613 31.620970249818598 20.511817145226495 62-63 18.419878843201527 29.33695697273972 31.71341438114601 20.529749802912743 64-65 18.411358483321834 29.299252349055966 31.74066214582566 20.54872702179654 66-67 18.61999366993374 29.099366474516298 31.250291857563134 21.03034799798683 68-69 18.589326158979055 29.19960461490166 31.177567862767123 21.033501363352162 70-71 19.01355831945331 29.097461122958855 30.70261463343763 21.186365924150206 72-73 18.865345003813484 28.654332069088966 31.04100406253081 21.439318864566744 74-75 18.504184154686616 28.96794309757148 30.731876877422167 21.79599587031974 76-77 18.469247748016464 28.70063208567523 30.819169547839575 22.01095061846873 78-79 18.638011419917955 28.59984130359245 30.89730890307591 21.864838373413683 80-81 18.770033505876498 28.63922573417288 30.459486934782838 22.13125382516779 82-83 18.851653296607818 28.242595892240697 30.332786081652024 22.57296472949946 84-85 19.205681043046145 27.840845724304458 30.24330654134344 22.71016669130596 86-87 19.44479743976099 27.59440751681194 30.1748753206134 22.78591972281367 88-89 19.92047785907515 27.063464685788986 30.130228528892268 22.885828926243605 90-91 20.16929893740661 26.759920305495598 30.045243234268636 23.025537522829154 92-93 20.546537961663482 26.947891076343584 29.80224706276055 22.703323899232387 94-95 20.594477076025818 26.427689338120835 29.92388078288122 23.053952802972123 96-97 20.67553789036454 26.05976011892871 29.935580283363734 23.329121707343017 98-99 21.125887260802788 25.880774978207626 29.717529367813704 23.275808393175875 100-101 22.240291318793332 24.922903747046483 30.13665943038254 22.70014550377765 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1973.0 1 1275.0 2 598.5 3 618.5 4 542.5 5 512.0 6 685.0 7 1094.5 8 1530.5 9 1616.0 10 1281.5 11 952.5 12 921.5 13 929.0 14 927.0 15 925.5 16 924.5 17 981.5 18 1013.0 19 1043.0 20 1129.5 21 1206.0 22 1310.0 23 1450.5 24 1628.0 25 1890.5 26 2299.5 27 2727.0 28 2925.5 29 3109.0 30 3456.5 31 3781.5 32 4184.5 33 4605.5 34 4860.0 35 5060.5 36 5395.5 37 5596.5 38 5488.0 39 5448.5 40 5419.0 41 5374.0 42 5372.5 43 5466.0 44 5556.0 45 5470.5 46 5526.0 47 5788.5 48 6057.5 49 5929.5 50 5482.5 51 4977.5 52 4330.5 53 3889.0 54 3572.0 55 3231.0 56 2957.5 57 2640.0 58 2393.0 59 2283.5 60 2045.0 61 1841.0 62 1630.5 63 1258.5 64 1022.0 65 900.5 66 807.0 67 742.5 68 670.5 69 612.0 70 557.5 71 494.0 72 443.5 73 396.0 74 333.5 75 260.0 76 205.5 77 166.0 78 134.5 79 109.5 80 87.0 81 60.5 82 35.5 83 26.0 84 22.0 85 17.5 86 14.0 87 9.0 88 6.0 89 4.0 90 1.5 91 0.5 92 1.5 93 1.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.265054638456173 2 0.1653362267173009 3 0.19892014776925265 4 0.16481955100880932 5 0.2407708801570694 6 0.19220336355886228 7 0.2381875016146116 8 0.24387093440801882 9 0.15241933400501176 10-11 0.20899532408483815 12-13 0.21467875687824536 14-15 0.17308636234467437 16-17 0.2844299775246067 18-19 0.22992069027874654 20-21 0.20202020202020202 22-23 0.2441292722622646 24-25 0.23198739311271283 26-27 0.33868092691622104 28-29 0.3076803844067271 30-31 0.2854633289415898 32-33 0.19427006639282854 34-35 0.36348136092381617 36-37 0.39887364695548844 38-39 0.3794983078870547 40-41 0.3391976026247126 42-43 0.3745898886563848 44-45 0.3851817406804619 46-47 0.3926735384535896 48-49 0.40843214756258234 50-51 0.401715363352192 52-53 0.4019737012064378 54-55 0.3960319305587848 56-57 0.37588157792761373 58-59 0.35159781962851017 60-61 0.312588803637397 62-63 0.3807899971582836 64-65 0.41747397246118473 66-67 0.4205740267121341 68-69 0.42444909452582086 70-71 0.4247074323800667 72-73 0.41747397246118473 74-75 0.41024051254230276 76-77 0.39809863339275103 78-79 0.37407321294789325 80-81 0.383890051409233 82-83 0.39448190343331013 84-85 0.39448190343331013 86-87 0.38776511922291973 88-89 0.3970652819757679 90-91 0.4179906481696763 92-93 0.4156656074814642 94-95 0.42419075667157513 96-97 0.42599912165129555 98-99 0.42212405383760876 100-101 0.3960319305587848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 193545.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.560923001638734 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87327825043597 48.37199454013118 2 12.419478511415523 15.042701637006845 3 3.726522418334676 6.770449117219406 4 1.4762832752235195 3.5762031103767447 5 0.7146086499983664 2.1638679714428033 6 0.4234868026858859 1.5388050989802067 7 0.2707032412495091 1.1475826704723888 8 0.20723195533666686 1.0040126792498332 9 0.11997498335475326 0.653921615616309 >10 0.6831607722668003 7.265767897157042 >50 0.05255081865128739 2.1839334642044927 >100 0.024788122005324237 2.4343322144308535 >500 0.0029745746406389085 0.9643654505120747 >1k 0.004957624401064848 6.882062533199805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 1.7654808959156787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2638 1.3629905190007492 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2193 1.1330698287220025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1833 0.9470665736650391 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1380 0.7130124777183601 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 575 0.29708853238265004 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 527 0.2722880983750549 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 504 0.2604045570797489 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 385 0.19892014776925265 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.16998630809372497 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.15500271254746958 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 238 0.12296881862099253 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.166757084915653E-4 0.0 20-21 0.0 0.0 0.0 0.0025833785424578264 0.0 22-23 0.0 0.0 0.0 0.0077501356273734796 0.0 24-25 0.0 0.0 0.0 0.016275284817484307 0.0 26-27 0.0 0.0 0.0 0.03539228603167222 0.0 28-29 0.0 0.0 0.0 0.07853470769071792 0.0 30-31 0.0 0.0 0.0 0.17205301092769124 0.0 32-33 0.0 0.0 0.0 0.31646387145108373 0.0 34-35 0.0 0.0 0.0 0.5345010204345243 0.0 36-37 0.0 0.0 0.0 0.8439897698209718 0.0 38-39 0.0 0.0 0.0 1.246738484590147 0.0 40-41 0.0 0.0 0.0 1.6636957813428401 0.0 42-43 0.0 0.0 0.0 2.037252318582242 0.0 44-45 0.0 0.0 0.0 2.477976697925547 0.0 46-47 0.0 0.0 0.0 2.94763491694438 0.0 48-49 0.0 0.0 0.0 3.435118447906172 0.0 50-51 0.0 0.0 0.0 3.955152548502932 0.0 52-53 0.0 0.0 0.0 4.448319512258131 0.0 54-55 0.0 0.0 0.0 4.958278436539306 0.0 56-57 0.0 0.0 0.0 5.528688418713994 0.0 58-59 0.0 0.0 0.0 6.1517993231548225 0.0 60-61 0.0 0.0 0.0 6.828644501278772 0.0 62-63 0.0 0.0 0.0 7.477072515435687 0.0 64-65 0.0 0.0 0.0 8.161926167041257 0.0 66-67 0.0 0.0 0.0 8.897155700224754 0.0 68-69 0.0 0.0 0.0 9.68017773644372 0.0 70-71 0.0 0.0 0.0 10.516675708491565 0.0 72-73 0.0 0.0 0.0 11.334056679325222 0.0 74-75 0.0 0.0 0.0 12.19354672040094 0.0 76-77 0.0 0.0 0.0 13.041928233744091 0.0 78-79 0.0 0.0 0.0 14.013536903562478 0.0 80-81 0.0 0.0 0.0 15.040946549897956 0.0 82-83 0.0 0.0 0.0 16.052339249270197 0.0 84-85 0.0 0.0 0.0 17.052106745201375 0.0 86-87 0.0 0.0 0.0 18.031982226355627 0.0 88-89 0.0 0.0 0.0 19.04208323645664 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1990 0.0 34.87467 1 GTATCAA 2960 0.0 31.957415 1 ACTTGGC 60 0.003968929 31.624105 8 GGGTAAC 65 0.0058114175 29.252092 1 GGAGTGC 65 0.0058706473 29.19148 8 CACCAGA 100 4.9075723E-5 28.520788 1 ACAGTGC 90 8.990819E-4 26.35342 8 TAGAATG 90 8.990819E-4 26.35342 5 TATCAAC 3565 0.0 26.34603 2 TGGGATC 165 8.443749E-8 25.874268 6 TCAACGC 3625 0.0 25.517382 4 ATCAACG 3645 0.0 25.50751 3 CAACGCA 3635 0.0 25.316683 5 AACGCAG 3680 0.0 25.136007 6 CTTGGCT 95 0.0012324854 24.966398 9 GTTAGTA 60 1.7115219E-4 23.742676 26-27 ATATGTA 100 0.0016616405 23.718079 5 GAGTACT 2340 0.0 22.925018 12-13 TGGGATA 105 0.0022068375 22.588646 6 ACGCAGA 4100 0.0 22.561098 7 >>END_MODULE