FastQCFastQC Report
Thu 26 May 2016
SRR938864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193545
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32071.6569789971324498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28611.4782092019943682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21971.1351365315559687No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20041.0354181198170969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19981.0323180655661475No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.6458446356144566No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT11220.5797101449275363RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.3156888578883464No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT5580.28830504533829343RNA PCR Primer, Index 33 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.28210493683639465No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG4690.2423209072825441RNA PCR Primer, Index 33 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.22423725748533935No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4120.2128703918985249No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.16740292955126715No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.16430287530031776No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2490.12865225141439976No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT2280.11780206153607688RNA PCR Primer, Index 33 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2260.11676871011909375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.037.2242131
TCTAGAT550.002583331734.5209163
ATCGCTC550.002589891734.503088
ATAAGCC600.003966670531.627829
CGGGAGT804.4986635E-429.6664144
GTATCAA31050.027.4134141
AAGCCGA350.00841391927.12357736-37
ACGCCCG350.00841922727.12007342-43
ATGGGAG3900.026.775845
GTATAGC908.8633475E-426.4179551
TGTGAGC950.001227943924.9822433
TTCTCTA1000.001655526523.7331312
TCTGATT1000.00166051823.7208678
ATGGGGG2052.660272E-823.1542765
TATCAAC37950.022.2634892
GTACATG19550.022.1343271
TACATGG19650.021.7402732
ATCAACG39050.021.6363493
TCGGGAG1100.00288066721.5755733
GTCGGGA1100.00288066721.5755732
Produced by FastQC (version 0.11.5)