##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938864_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193545 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874458136350718 31.0 31.0 34.0 30.0 34.0 2 32.04861401741197 33.0 31.0 34.0 30.0 34.0 3 32.067942855666644 33.0 31.0 34.0 30.0 34.0 4 35.78414838926348 37.0 35.0 37.0 35.0 37.0 5 35.70843989769821 37.0 35.0 37.0 33.0 37.0 6 35.768746286393345 37.0 35.0 37.0 33.0 37.0 7 35.675842827249475 37.0 35.0 37.0 33.0 37.0 8 35.68647084657315 37.0 35.0 37.0 33.0 37.0 9 37.24744633031078 39.0 37.0 39.0 33.0 39.0 10-11 37.19535508538066 39.0 37.0 39.0 33.0 39.0 12-13 37.08543232839908 39.0 37.0 39.0 33.0 39.0 14-15 38.131181379007465 40.0 37.0 41.0 33.0 41.0 16-17 38.06767676767677 40.0 37.0 41.0 33.0 41.0 18-19 38.258518690743756 40.0 37.5 41.0 33.0 41.0 20-21 38.277976697925546 40.0 37.5 41.0 33.0 41.0 22-23 38.13143455010463 40.0 37.0 41.0 33.0 41.0 24-25 37.97502647963006 40.0 37.0 41.0 33.0 41.0 26-27 37.70255754475703 40.0 36.5 41.0 33.0 41.0 28-29 37.37794828076158 39.5 36.0 41.0 32.0 41.0 30-31 36.921555710558266 39.0 35.0 41.0 31.5 41.0 32-33 36.397116949546614 38.0 35.0 41.0 30.5 41.0 34-35 36.05657599007982 38.0 35.0 41.0 30.0 41.0 36-37 35.70138469089876 38.0 35.0 41.0 29.5 41.0 38-39 35.34545712883309 38.0 35.0 41.0 28.0 41.0 40-41 34.98144875868661 38.0 35.0 40.5 26.0 41.0 42-43 34.48204293575138 38.0 34.0 40.0 23.5 41.0 44-45 33.85002454209615 38.0 33.5 40.0 19.5 41.0 46-47 32.999739078767206 38.0 33.0 40.0 5.5 41.0 48-49 32.26389728486915 37.5 32.5 40.0 2.0 41.0 50-51 31.78059366038906 37.0 31.5 40.0 2.0 41.0 52-53 31.469890723087655 37.0 31.0 40.0 2.0 41.0 54-55 31.115541605311428 36.0 31.0 40.0 2.0 41.0 56-57 30.68647084657315 36.0 30.0 40.0 2.0 41.0 58-59 30.248629517683227 35.0 29.5 40.0 2.0 41.0 60-61 29.743173422201554 35.0 28.0 39.0 2.0 41.0 62-63 29.243938102250123 35.0 27.0 39.0 2.0 40.5 64-65 28.72902167454597 35.0 26.0 39.0 2.0 40.0 66-67 28.181180087318197 35.0 25.0 38.0 2.0 40.0 68-69 27.79006432612571 35.0 23.0 38.0 2.0 40.0 70-71 27.378813712573304 34.0 22.5 37.0 2.0 40.0 72-73 26.90210287013356 34.0 20.5 36.5 2.0 39.0 74-75 26.42982252189413 34.0 18.5 36.0 2.0 39.0 76-77 24.663824950269962 31.5 12.5 34.5 2.0 36.5 78-79 25.428636751143145 33.0 8.0 35.0 2.0 37.0 80-81 25.394670490066908 33.0 4.5 35.0 2.0 37.0 82-83 25.030879123717998 33.0 2.0 35.0 2.0 36.5 84-85 24.609307912888475 33.0 2.0 35.0 2.0 36.0 86-87 24.034754191531686 32.5 2.0 35.0 2.0 36.0 88-89 23.44297966881087 32.0 2.0 35.0 2.0 35.0 90-91 22.89971582836033 31.0 2.0 35.0 2.0 35.0 92-93 22.574866310160427 31.0 2.0 34.0 2.0 35.0 94-95 22.202673796791444 31.0 2.0 34.0 2.0 35.0 96-97 21.64498437055982 31.0 2.0 34.0 2.0 35.0 98-99 20.93886693017128 30.0 2.0 34.0 2.0 35.0 100-101 18.171675837660494 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.029977514501140945 1101 2 0.14686183675706843 1101 3 0.15055638312301767 1101 4 0.2395418454460625 1101 5 0.19080258003350536 1101 6 0.22687024305630388 1101 7 0.2068919656043633 1101 8 0.29353940478406315 1101 9 0.29211585327850287 1101 10-11 0.2165180101420674 1101 12-13 0.3345258771556061 1101 14-15 0.22872103415169676 1101 16-17 0.049678603669676136 1101 18-19 0.2883928128828117 1101 20-21 0.36102259119954283 1101 22-23 0.06581600364034301 1101 24-25 -0.0011015984593782946 1101 26-27 0.08217521007104267 1101 28-29 0.16021303268102827 1101 30-31 0.16109536646252565 1101 32-33 0.36651180699447394 1101 34-35 0.2896417441118544 1101 36-37 0.5792549027522966 1101 38-39 0.3766390281839733 1101 40-41 0.22054051430050237 1101 42-43 0.623471768418284 1101 44-45 0.5272872135003013 1101 46-47 0.0590257869126134 1101 48-49 0.001552422535176845 1101 50-51 -0.3356870283311544 1101 52-53 -0.48859274832728516 1101 54-55 -0.23035997993855872 1101 56-57 -0.29143980095068756 1101 58-59 -0.5677214424547437 1101 60-61 -0.4851321668560544 1101 62-63 -0.7021776704593918 1101 64-65 -0.35443376946031435 1101 66-67 -0.299804579809301 1101 68-69 -0.24110354876972195 1101 70-71 -0.6403635844035804 1101 72-73 -0.43688170500658785 1101 74-75 -0.9541699632740226 1101 76-77 -0.4192767556823789 1101 78-79 -0.6298327468659828 1101 80-81 -1.144914319932301 1101 82-83 -0.9410907884241588 1101 84-85 -1.1607659024567738 1101 86-87 -1.2670279210450701 1101 88-89 -1.3428279217099934 1101 90-91 -0.8641357722023209 1101 92-93 -0.7561868788465382 1101 94-95 -0.6884082401676928 1101 96-97 -1.0357098819561017 1101 98-99 -1.156607782857833 1101 100-101 -0.6432285519713723 1102 1 0.15105937371806633 1102 2 -0.09146061427256313 1102 3 -0.23093811991844504 1102 4 -0.029753316114295103 1102 5 -0.10360559006527126 1102 6 -0.02585033286869276 1102 7 0.23197707079997087 1102 8 -0.0941496403635469 1102 9 0.36734326552248575 1102 10-11 0.49267739403626365 1102 12-13 0.31581668579448774 1102 14-15 0.28419287967879114 1102 16-17 0.3666745297816263 1102 18-19 0.10688156783565006 1102 20-21 0.2713970540601949 1102 22-23 0.3375248043546648 1102 24-25 0.14918580597166908 1102 26-27 0.15981626146899686 1102 28-29 0.46781948393388717 1102 30-31 0.218269315901658 1102 32-33 0.1684678313261827 1102 34-35 -0.12290917948879354 1102 36-37 -0.4574519447280352 1102 38-39 -0.19748620201860945 1102 40-41 -0.3789209511830691 1102 42-43 -0.23489086342501508 1102 44-45 -0.24729563152452982 1102 46-47 -0.05830776964747031 1102 48-49 -0.2412345633457349 1102 50-51 -0.25476733415179353 1102 52-53 -0.24389438346317505 1102 54-55 -0.7021776036898864 1102 56-57 -0.9088710192106362 1102 58-59 -0.6731821266447078 1102 60-61 -0.747883994525008 1102 62-63 -0.9015889099258771 1102 64-65 -1.2221440211484662 1102 66-67 -0.8265499339027222 1102 68-69 -0.9457187616722287 1102 70-71 -1.0445574568294944 1102 72-73 -0.4583254239641157 1102 74-75 -0.5194916888167391 1102 76-77 -1.295609140302055 1102 78-79 -0.8485391478928257 1102 80-81 -0.676807840776096 1102 82-83 -0.5864253495045766 1102 84-85 0.049443930681235315 1102 86-87 -0.0671004697364026 1102 88-89 -0.5894934722429497 1102 90-91 -0.05958473698830247 1102 92-93 0.1940196058903325 1102 94-95 0.10160515343686072 1102 96-97 -0.07227721272232657 1102 98-99 -0.4871507819091221 1102 100-101 0.24623235544533983 1103 1 0.3283626438390179 1103 2 0.1682377581329888 1103 3 -0.011496578929943269 1103 4 -0.16479885889464185 1103 5 0.2773709666018931 1103 6 0.3036106197966788 1103 7 0.20085865957106108 1103 8 0.3685052797499395 1103 9 0.3480809092435564 1103 10-11 0.41709131071536376 1103 12-13 0.25184774447747316 1103 14-15 0.47403907946974755 1103 16-17 0.29670290069397254 1103 18-19 0.24861667310666746 1103 20-21 0.3036652338421888 1103 22-23 0.47434726217159806 1103 24-25 0.179542682184902 1103 26-27 -0.20631782842200153 1103 28-29 0.15983083229883022 1103 30-31 0.4830445884117367 1103 32-33 0.14536793585060082 1103 34-35 -0.3705275160574004 1103 36-37 -0.15854198504459305 1103 38-39 -0.17246007091513604 1103 40-41 -0.28026483650485545 1103 42-43 -0.1816879367414188 1103 44-45 0.14744828366136886 1103 46-47 0.13737686526368975 1103 48-49 -0.14289202190926886 1103 50-51 -0.251138843782968 1103 52-53 0.025644215909675694 1103 54-55 -0.13598378556236312 1103 56-57 -1.0646721241830122 1103 58-59 -0.7742870990203983 1103 60-61 -0.7387903705142556 1103 62-63 -0.3617053799871002 1103 64-65 -0.16404337906992694 1103 66-67 0.10498657498185082 1103 68-69 -0.04697305463922774 1103 70-71 -0.1046141986541933 1103 72-73 0.1252941071692284 1103 74-75 0.22382621472215547 1103 76-77 0.0680014315958104 1103 78-79 0.048190731157493616 1103 80-81 -0.26702734204532064 1103 82-83 -0.6088085061418802 1103 84-85 -0.31032910201997055 1103 86-87 -0.26418871820586887 1103 88-89 -0.41656529544737 1103 90-91 -0.13818709625364534 1103 92-93 -0.24399741665707708 1103 94-95 0.2199865182270635 1103 96-97 0.46427646803024913 1103 98-99 -0.18292500917505805 1103 100-101 0.016517557774736957 1104 1 -0.08519288059733654 1104 2 -0.13740570916850814 1104 3 -0.2475699580085049 1104 4 0.0067737499836653114 1104 5 -0.27229994783586164 1104 6 0.04591561909649755 1104 7 -0.1424826091588116 1104 8 0.0031222801700465652 1104 9 -0.1647324222226274 1104 10-11 -0.14955201654971972 1104 12-13 -0.6012960729150478 1104 14-15 -0.20355509501562352 1104 16-17 -0.4381661652631692 1104 18-19 -0.20056411389794704 1104 20-21 -0.3119768330952226 1104 22-23 -0.3104727910422369 1104 24-25 -0.1365755085446878 1104 26-27 -0.04590612137816663 1104 28-29 -0.3168110946021656 1104 30-31 -0.6006411698854137 1104 32-33 -0.530083681569927 1104 34-35 -0.17435051003584334 1104 36-37 0.023869441926414936 1104 38-39 -0.3146390374464261 1104 40-41 0.14053057030091765 1104 42-43 -0.18552493436080653 1104 44-45 0.44743428002042407 1104 46-47 0.5070029680504149 1104 48-49 0.3213566827539509 1104 50-51 0.15722132986218185 1104 52-53 0.5260223142152363 1104 54-55 0.3145973655369225 1104 56-57 0.8451724354024783 1104 58-59 0.6707906211868568 1104 60-61 0.7186805719307792 1104 62-63 0.7390088057011788 1104 64-65 0.3153992760204005 1104 66-67 0.020614638278306785 1104 68-69 0.13357588808587906 1104 70-71 0.18170424414093134 1104 72-73 0.25881882359549735 1104 74-75 0.6698421788728375 1104 76-77 0.6774678928757432 1104 78-79 0.32340428668193155 1104 80-81 0.4704884120507451 1104 82-83 0.5875505472119933 1104 84-85 1.0874023081466717 1104 86-87 1.1796146706752424 1104 88-89 0.7368025802003011 1104 90-91 0.8206001884403769 1104 92-93 0.6753696187721339 1104 94-95 1.2270023423413328 1104 96-97 1.3100403666438893 1104 98-99 1.8825140049686198 1104 100-101 1.5286587144754797 1105 1 -0.2512959621353765 1105 2 -0.25935825893671094 1105 3 -0.06597737551600602 1105 4 0.019127172399812764 1105 5 -0.03343984420891388 1105 6 0.13803451737847183 1105 7 0.15064528830505708 1105 8 0.03521936225349265 1105 9 0.22645928762193535 1105 10-11 -0.26370527534437826 1105 12-13 0.10988472883024514 1105 14-15 0.06984277790501636 1105 16-17 0.14627615816196737 1105 18-19 -0.0668171818008716 1105 20-21 -0.08488384154899364 1105 22-23 -0.09101546898060775 1105 24-25 -0.29549287969276605 1105 26-27 -0.06862223809483226 1105 28-29 0.24439697739128974 1105 30-31 -0.31482169892822753 1105 32-33 -0.33016549231440706 1105 34-35 -0.3134151405239791 1105 36-37 -0.4091820988426065 1105 38-39 -0.19002763848270376 1105 40-41 -0.456865120999872 1105 42-43 -0.8018870832563536 1105 44-45 -0.7851263962817328 1105 46-47 -1.3723670892171107 1105 48-49 -1.8086814955934756 1105 50-51 -1.1330022861727294 1105 52-53 -1.8948394256265964 1105 54-55 -1.9867661468710445 1105 56-57 -2.2751984399724883 1105 58-59 -1.866392362178292 1105 60-61 -1.7207249366593516 1105 62-63 -1.7661861765731608 1105 64-65 -1.8389366934369242 1105 66-67 -2.162723240096387 1105 68-69 -2.1563613903433563 1105 70-71 -1.6142870293796534 1105 72-73 -1.9409221090469906 1105 74-75 -2.2541965448795516 1105 76-77 -2.0167780847626524 1105 78-79 -2.0326769786575802 1105 80-81 -1.6731439850040708 1105 82-83 -1.538751607137609 1105 84-85 -1.6111114633286476 1105 86-87 -1.8942740667122706 1105 88-89 -1.7507758217631562 1105 90-91 -2.339680695115664 1105 92-93 -2.1116361079799795 1105 94-95 -2.10220409343723 1105 96-97 -1.7764063200209605 1105 98-99 -1.5903930034851612 1105 100-101 -0.6070244052409102 1106 1 0.08529283606332783 1106 2 0.16023082071428973 1106 3 0.07331336470353023 1106 4 -0.0536845360156164 1106 5 -0.2575040153319108 1106 6 -0.039618170490932414 1106 7 -0.06019062500763539 1106 8 -0.36480878297589214 1106 9 0.05801008976096966 1106 10-11 0.17997612065900626 1106 12-13 -0.022425994502143 1106 14-15 0.03383095690868032 1106 16-17 0.10775941175048587 1106 18-19 0.2075847320747286 1106 20-21 -0.01093225134353304 1106 22-23 0.178264013147178 1106 24-25 0.26241592976403183 1106 26-27 0.199376636095991 1106 28-29 -0.10686955793097042 1106 30-31 -0.589350266336055 1106 32-33 -0.1853301419062987 1106 34-35 -0.3954444115625364 1106 36-37 -0.9229732612405783 1106 38-39 -0.5694394031885821 1106 40-41 -0.12423072752957154 1106 42-43 -0.7722501567154083 1106 44-45 -0.20964089107486217 1106 46-47 -0.31471158682476386 1106 48-49 -0.26107384009108614 1106 50-51 -0.04216355833709429 1106 52-53 0.042453003306700765 1106 54-55 -0.124002285345572 1106 56-57 -0.2603940493167052 1106 58-59 -0.04915340918082478 1106 60-61 -0.2143359695892677 1106 62-63 -0.28491682672795804 1106 64-65 -0.7468877207377957 1106 66-67 -0.7048847937130454 1106 68-69 -0.5679009343906358 1106 70-71 -0.9545347073982313 1106 72-73 -0.6123124833785418 1106 74-75 -0.18114560201436447 1106 76-77 -0.06485013654987526 1106 78-79 -0.392161920959861 1106 80-81 -0.12887153918363836 1106 82-83 0.341588950137929 1106 84-85 0.14122459071019478 1106 86-87 0.08828996368458064 1106 88-89 0.20322080080931926 1106 90-91 0.15532352666874516 1106 92-93 -0.14581848906638584 1106 94-95 0.25054006642767135 1106 96-97 -0.23728445117772878 1106 98-99 0.7263393353834005 1106 100-101 1.4320833909876285 1107 1 -0.19400117075277734 1107 2 -0.24103265217368985 1107 3 -0.009227989096586953 1107 4 -0.03985837436866291 1107 5 -0.06504606182549821 1107 6 -0.19760769696567593 1107 7 -0.09066913765622076 1107 8 -0.30257720169523594 1107 9 -0.3098101424298747 1107 10-11 -0.3853033118865028 1107 12-13 -0.3370473682518309 1107 14-15 -0.1802390328394594 1107 16-17 -0.47443559531498636 1107 18-19 0.014461792837799692 1107 20-21 -0.5477281284320057 1107 22-23 -0.4944106148764291 1107 24-25 -0.6598915707208519 1107 26-27 -0.3619682975439673 1107 28-29 -0.42821005833273773 1107 30-31 -0.35452718024810537 1107 32-33 -0.356508552036253 1107 34-35 -1.0137287483778081 1107 36-37 -1.3103274492652943 1107 38-39 -0.928628674752126 1107 40-41 -0.4864124348804282 1107 42-43 -0.6932969550861898 1107 44-45 -0.4903895541764669 1107 46-47 -0.6639814879081385 1107 48-49 -0.54392829133932 1107 50-51 -0.8168719343865263 1107 52-53 -0.8548067013288048 1107 54-55 -0.45483268627654994 1107 56-57 -0.5719260101933763 1107 58-59 -0.3551161145644386 1107 60-61 0.2031365304800019 1107 62-63 0.31672901295506506 1107 64-65 0.49933709985459274 1107 66-67 0.717505829988152 1107 68-69 0.8149230383449471 1107 70-71 0.6143467311875668 1107 72-73 0.40130587022762043 1107 74-75 0.5739872565930462 1107 76-77 0.14378913639906088 1107 78-79 0.03324884626742275 1107 80-81 -0.1460918988300861 1107 82-83 -0.24664634397971597 1107 84-85 0.009682100892785428 1107 86-87 0.055262339069024335 1107 88-89 -0.21138394829710805 1107 90-91 -0.0032340084508177824 1107 92-93 -0.18473400817087438 1107 94-95 -0.21082149185555465 1107 96-97 -0.2832238820607742 1107 98-99 -0.05530982922827121 1107 100-101 0.5416120823511257 1108 1 0.024412539888913898 1108 2 0.1173913072865389 1108 3 -0.1531954206287871 1108 4 -0.05582944992523409 1108 5 0.06326345249438248 1108 6 0.1842462004322627 1108 7 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-1.6512720922136914 2316 94-95 -1.4959439496857811 2316 96-97 -1.815590182761511 2316 98-99 -1.6358717885535583 2316 100-101 -1.417724613912366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 9.0 5 23.0 6 89.0 7 141.0 8 283.0 9 449.0 10 821.0 11 1339.0 12 1715.0 13 2335.0 14 2492.0 15 3351.0 16 4309.0 17 5652.0 18 5081.0 19 3103.0 20 2677.0 21 2650.0 22 2847.0 23 2964.0 24 3149.0 25 3190.0 26 3221.0 27 3409.0 28 3847.0 29 4519.0 30 5267.0 31 6082.0 32 7131.0 33 8776.0 34 11418.0 35 16103.0 36 19488.0 37 28049.0 38 24880.0 39 2683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08055595832384 22.092818526420448 16.36441783872237 18.46220767653334 2 15.618589991991527 23.77431605053088 40.1653362267173 20.441757730760287 3 18.30633702756465 30.260146219225504 33.5865044304942 17.847012322715646 4 11.505334676690175 21.12221963884368 36.818827662817434 30.55361802164871 5 10.557731862861631 40.481038563706086 33.78639572502041 15.17483384841187 6 24.819932003017495 39.276007812419266 18.26876375698829 17.635296427574946 7 21.658529024257923 39.15833527086724 21.34387351778656 17.839262187088273 8 24.742566328244077 33.7518406572115 21.42602495543672 20.0795680591077 9 25.67723268490532 17.225451445400296 21.968017773644373 35.129298096050015 10-11 22.875558655609808 29.187527448397017 26.806685783667884 21.130228112325298 12-13 25.68627450980392 28.096308352062827 27.667725851869072 18.549691286264174 14-15 22.645379627476814 28.254411118861245 29.136634891110596 19.963574362551345 16-17 18.287834203530565 31.73218765258592 29.546317657727016 20.4336604861565 18-19 17.613991578185953 28.075124648014672 35.43775349401948 18.873130279779897 20-21 19.99560825647782 26.484280141569144 35.59327288227544 17.926838719677594 22-23 19.269678886047174 27.939497274535636 35.381435841793895 17.40938799762329 24-25 18.66747613573246 28.62394564501337 35.682343671286674 17.0262345479675 26-27 18.10236590696545 28.85822642230845 36.12567879674074 16.913728873985356 28-29 17.62148337595908 28.746028055490967 36.24686765351727 17.38562091503268 30-31 18.707794053062596 28.383321708129895 35.86891937275569 17.039964866051825 32-33 17.76253584437728 28.940556459738044 35.910253429435016 17.386654266449664 34-35 17.874277564919044 28.20500233814483 36.337612612030725 17.5831074849054 36-37 17.46603482044577 28.93564573891384 35.71188639170676 17.886433048933633 38-39 17.54922226241538 28.011213890755 35.86765541832463 18.571908428504987 40-41 17.6083339361933 28.12135054412418 36.103130393443635 18.167185126238877 42-43 17.94264306937088 28.320113680403047 35.405761529518145 18.331481720707917 44-45 17.43745947105038 28.775231289511687 34.86122629298652 18.92608294645141 46-47 18.546266204409356 28.317221495998307 33.54241693060457 19.59409536898777 48-49 18.1642507085117 28.692075777812796 33.03356679127516 20.110106722400335 50-51 17.81575344235191 29.455682141104134 32.621354207031956 20.107210209512 52-53 17.90668836704642 29.950915807693303 32.578728461081404 19.563667364178876 54-55 17.539848614017412 29.712986643932936 31.952517502389625 20.794647239660026 56-57 18.524871798183863 30.172184403942286 30.51190307038506 20.791040727488795 58-59 17.741185466870583 30.767680734096643 31.324918364816266 20.166215434216507 60-61 18.1903201971716 31.508703762071335 30.322883996341787 19.97809204441528 62-63 17.455474550612017 31.901250897742596 30.29564795056345 20.34762660108194 64-65 17.49100312856032 31.825286311063117 30.335409567555978 20.348300992820587 66-67 17.744748243816865 31.43561350794836 29.848525167867596 20.971113080367175 68-69 17.698301161492992 31.145114289257226 29.92601165626421 21.230572892985574 70-71 18.11314710570437 30.79731017931277 29.443357987439583 21.64618472754328 72-73 17.887571365832237 30.33997261618745 29.716861711746624 22.05559430623369 74-75 17.479139218269655 30.67684517812395 29.128109741920483 22.71590586168591 76-77 17.27160404474865 30.162430984925248 29.776256746417417 22.789708223908683 78-79 17.2868728415661 30.260887560953524 29.812321343111766 22.639918254368606 80-81 17.13026472495421 30.351011803731005 29.28715178476856 23.231571686546225 82-83 17.139262481851393 29.85749789450297 29.28991789853313 23.713321725112507 84-85 17.220543026169626 29.66416078948048 29.061458575525073 24.05383760882482 86-87 17.267327449555616 29.72255910714147 28.797066558403777 24.213046884899136 88-89 17.491011739821296 29.361217893053315 28.75284163813148 24.39492872899391 90-91 17.321964805277652 29.177752537432646 28.771140950039676 24.72914170725003 92-93 17.364953886693016 29.51432483401793 28.489240228370665 24.631481050918392 94-95 17.33421168203777 29.0475083313958 28.599292154279365 25.018987832287067 96-97 17.046939988116456 28.812679221886384 28.817329303262806 25.32305148673435 98-99 17.194192565036552 28.78529540933633 28.460306388695134 25.560205636931983 100-101 17.538493334711173 28.41712307533327 29.002531776376976 25.041851813578585 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1805.0 1 1165.0 2 564.0 3 585.5 4 479.0 5 447.5 6 655.5 7 1070.5 8 1448.0 9 1520.5 10 1197.0 11 893.5 12 859.0 13 879.0 14 873.5 15 865.0 16 896.5 17 944.0 18 1024.5 19 1049.5 20 1112.0 21 1257.0 22 1366.0 23 1547.5 24 1716.0 25 1894.5 26 2364.0 27 2794.5 28 2973.5 29 3231.0 30 3560.5 31 3831.5 32 4204.0 33 4589.0 34 4923.0 35 5190.5 36 5438.0 37 5619.0 38 5548.5 39 5408.5 40 5557.5 41 5732.0 42 5705.0 43 5950.5 44 6208.5 45 6153.5 46 6039.5 47 6216.0 48 6616.0 49 6402.0 50 5880.5 51 5277.5 52 4211.5 53 3461.0 54 3143.5 55 2822.5 56 2601.5 57 2316.5 58 2057.0 59 2000.0 60 1794.5 61 1586.5 62 1378.0 63 1018.0 64 812.0 65 745.0 66 683.5 67 618.5 68 549.0 69 485.5 70 429.0 71 388.5 72 368.0 73 355.0 74 284.5 75 202.0 76 167.5 77 132.5 78 95.0 79 71.5 80 52.0 81 30.5 82 17.5 83 13.0 84 9.0 85 6.0 86 3.5 87 1.5 88 2.0 89 2.5 90 1.0 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22578728461081404 2 0.0 3 0.0 4 0.0 5 0.024283758299103567 6 0.003616729959440957 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003358392105195174 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012916892712289132 26-27 0.002066702833966261 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008008473481619262 36-37 0.01963367692267948 38-39 0.018083649797204785 40-41 0.011883541295306001 42-43 0.010333514169831306 44-45 0.005425094939161435 46-47 0.0010333514169831306 48-49 0.002841716396703609 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0012916892712289132 58-59 5.166757084915653E-4 60-61 0.004133405667932522 62-63 0.002066702833966261 64-65 0.002841716396703609 66-67 0.007491797773127696 68-69 5.166757084915653E-4 70-71 2.5833785424578265E-4 72-73 0.0 74-75 0.0 76-77 0.05631765222558062 78-79 0.1356273734790359 80-81 0.0023250406882120438 82-83 0.002066702833966261 84-85 0.0 86-87 0.09739337105066005 88-89 0.33816425120772947 90-91 0.3782066186158258 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0025833785424578264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 193545.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.55414416418149 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75072621259679 45.32430649473781 2 13.14125276535368 15.126671123102287 3 3.873899281560293 6.688768731853205 4 1.524136525047663 3.508814931539512 5 0.7840898443644877 2.256380997011217 6 0.43672571812507527 1.508122496470576 7 0.30141746498091504 1.2143476963179596 8 0.18701655768587963 0.8610862337713663 9 0.16115230882808818 0.8347484893214235 >10 0.740106625679208 7.552238125504694 >50 0.05769648355119831 2.4280980716754 >100 0.031832542648937 3.333267383469507 >500 0.0029843008733378435 0.9818882793971495 >1k 0.006963368704454968 8.381260945827915 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3207 1.6569789971324498 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2861 1.4782092019943682 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2197 1.1351365315559687 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2004 1.0354181198170969 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1998 1.0323180655661475 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1250 0.6458446356144566 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1122 0.5797101449275363 RNA PCR Primer, Index 33 (95% over 22bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 611 0.3156888578883464 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 558 0.28830504533829343 RNA PCR Primer, Index 33 (95% over 24bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 546 0.28210493683639465 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 469 0.2423209072825441 RNA PCR Primer, Index 33 (96% over 29bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.22423725748533935 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 412 0.2128703918985249 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 324 0.16740292955126715 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 318 0.16430287530031776 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 249 0.12865225141439976 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 228 0.11780206153607688 RNA PCR Primer, Index 33 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 226 0.11676871011909375 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.166757084915653E-4 0.0 20-21 0.0 0.0 0.0 0.0025833785424578264 0.0 22-23 0.0 0.0 0.0 0.0077501356273734796 0.0 24-25 0.0 0.0 0.0 0.01756697408871322 0.0 26-27 0.0 0.0 0.0 0.0372006510113927 0.0 28-29 0.0 0.0 0.0 0.0818930997959131 0.0 30-31 0.0 0.0 0.0 0.17799478157534424 0.0 32-33 0.0 0.0 0.0 0.3260223720581777 0.0 34-35 0.0 0.0 0.0 0.5494846159807797 0.0 36-37 0.0 0.0 0.0 0.859231703221473 0.0 38-39 0.0 0.0 0.0 1.2666304993670723 0.0 40-41 0.0 0.0 0.0 1.686429512516469 0.0 42-43 0.0 0.0 0.0 2.066702833966261 0.0 44-45 0.0 0.0 0.0 2.5156940246454313 0.0 46-47 0.0 0.0 0.0 2.991552352166163 0.0 48-49 0.0 0.0 0.0 3.483685964504379 0.0 50-51 0.0 0.0 0.0 4.016895295667674 0.0 52-53 0.0 0.0 0.0 4.516520705779017 5.166757084915653E-4 54-55 0.0 0.0 0.0 5.0342297656875665 5.166757084915653E-4 56-57 0.0 0.0 0.0 5.616006613449069 5.166757084915653E-4 58-59 0.0 0.0 0.0 6.256426154124364 5.166757084915653E-4 60-61 0.0 0.0 0.0 6.942313157146916 5.166757084915653E-4 62-63 0.0 0.0 0.0 7.599524658348187 5.166757084915653E-4 64-65 0.0 0.0 0.0 8.298845229791521 5.166757084915653E-4 66-67 0.0 0.0 0.0 9.050091709938258 5.166757084915653E-4 68-69 0.0 0.0 0.0 9.853005760934149 5.166757084915653E-4 70-71 0.0 0.0 0.0 10.705520679945232 5.166757084915653E-4 72-73 0.0 0.0 0.0 11.539435273450618 5.166757084915653E-4 74-75 0.0 0.0 0.0 12.408483815133431 5.166757084915653E-4 76-77 0.0 0.0 0.0 13.273657289002557 5.166757084915653E-4 78-79 0.0 0.0 0.0 14.250174378051614 0.0010333514169831306 80-81 0.0 0.0 0.0 15.29437598491307 0.0010333514169831306 82-83 0.0 0.0 0.0 16.321010617685808 0.0010333514169831306 84-85 0.0 0.0 0.0 17.325944870701903 0.0010333514169831306 86-87 0.0 0.0 0.0 18.31124544679532 0.0010333514169831306 88-89 0.0 0.0 0.0 19.336588390296832 0.0010333514169831306 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2210 0.0 37.224213 1 TCTAGAT 55 0.0025833317 34.520916 3 ATCGCTC 55 0.0025898917 34.50308 8 ATAAGCC 60 0.0039666705 31.62782 9 CGGGAGT 80 4.4986635E-4 29.666414 4 GTATCAA 3105 0.0 27.413414 1 AAGCCGA 35 0.008413919 27.123577 36-37 ACGCCCG 35 0.008419227 27.120073 42-43 ATGGGAG 390 0.0 26.77584 5 GTATAGC 90 8.8633475E-4 26.417955 1 TGTGAGC 95 0.0012279439 24.982243 3 TTCTCTA 100 0.0016555265 23.733131 2 TCTGATT 100 0.001660518 23.720867 8 ATGGGGG 205 2.660272E-8 23.154276 5 TATCAAC 3795 0.0 22.263489 2 GTACATG 1955 0.0 22.134327 1 TACATGG 1965 0.0 21.740273 2 ATCAACG 3905 0.0 21.636349 3 TCGGGAG 110 0.002880667 21.575573 3 GTCGGGA 110 0.002880667 21.575573 2 >>END_MODULE