##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938863_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186351 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.905857293728225 31.0 31.0 34.0 30.0 34.0 2 32.12142972468739 33.0 31.0 34.0 30.0 34.0 3 32.184075201099915 34.0 31.0 34.0 30.0 34.0 4 35.854576415223356 37.0 35.0 37.0 35.0 37.0 5 35.78053844053402 37.0 35.0 37.0 35.0 37.0 6 35.829500844100515 37.0 35.0 37.0 35.0 37.0 7 35.77307989430791 37.0 35.0 37.0 33.0 37.0 8 35.75638861280737 37.0 35.0 37.0 33.0 37.0 9 37.35261264087971 39.0 37.0 39.0 34.0 39.0 10-11 37.282786707166416 39.0 37.0 39.0 33.0 39.0 12-13 37.24108480294335 39.0 37.0 39.0 33.0 39.0 14-15 38.351230200457294 40.0 38.0 41.0 33.0 41.0 16-17 38.305152969491175 40.0 38.0 41.0 33.0 41.0 18-19 38.43011163349343 40.0 38.0 41.0 33.5 41.0 20-21 38.535543698152765 40.0 38.0 41.0 34.0 41.0 22-23 38.47087178591178 40.0 38.0 41.0 34.0 41.0 24-25 38.38457342851171 40.0 38.0 41.0 34.0 41.0 26-27 38.19409669353182 40.0 37.5 41.0 33.0 41.0 28-29 38.0481381992187 40.0 37.0 41.0 33.0 41.0 30-31 37.76399672330747 40.0 37.0 41.0 33.0 41.0 32-33 37.45796283396399 40.0 36.0 41.0 32.5 41.0 34-35 37.318600947423356 40.0 36.0 41.0 32.0 41.0 36-37 37.15848758959564 40.0 35.5 41.0 31.5 41.0 38-39 37.017788314868014 40.0 35.0 41.0 31.0 41.0 40-41 36.864841464156484 40.0 35.0 41.0 31.0 41.0 42-43 36.61256655495846 40.0 35.0 41.0 30.5 41.0 44-45 36.23269502472384 39.0 35.0 41.0 30.0 41.0 46-47 35.766942041785605 39.0 35.0 41.0 28.0 41.0 48-49 35.35567139036687 39.0 35.0 41.0 26.0 41.0 50-51 35.11187201871977 39.0 35.0 41.0 24.0 41.0 52-53 34.97472775414377 39.0 35.0 41.0 24.0 41.0 54-55 34.78206312710081 39.0 35.0 40.5 23.5 41.0 56-57 34.5556189285252 39.0 34.5 40.0 23.0 41.0 58-59 34.31369002506917 38.0 34.0 40.0 22.0 41.0 60-61 33.97855810892213 38.0 34.0 40.0 21.5 41.0 62-63 33.66762610395129 38.0 33.5 40.0 19.5 41.0 64-65 33.352117523673 37.5 33.0 40.0 18.0 41.0 66-67 32.96284905763073 37.0 33.0 39.5 14.5 41.0 68-69 32.50288219961021 36.0 32.0 39.0 12.0 40.0 70-71 32.025065508694624 36.0 31.5 39.0 8.0 40.0 72-73 31.661457606761218 35.0 31.0 38.0 7.0 40.0 74-75 31.20835355347792 35.0 31.0 37.5 2.0 39.5 76-77 29.125624385105596 33.0 28.5 35.5 2.0 38.0 78-79 30.15937582757755 34.0 30.0 36.0 2.0 39.0 80-81 30.137974643595655 35.0 30.0 36.0 2.0 38.5 82-83 29.782701633909653 34.0 30.0 36.0 2.0 37.0 84-85 29.345556591782383 34.0 29.5 35.0 2.0 37.0 86-87 28.83981117396063 34.0 29.0 35.0 2.0 36.0 88-89 28.285584290902968 34.0 29.0 35.0 2.0 36.0 90-91 27.842602125642223 34.0 27.5 35.0 2.0 36.0 92-93 27.55104852788962 34.0 27.0 35.0 2.0 35.0 94-95 27.147410914349983 33.0 26.0 35.0 2.0 35.0 96-97 26.61952380930601 33.0 25.5 35.0 2.0 35.0 98-99 25.92854623983066 33.0 24.5 35.0 2.0 35.0 100-101 22.869715795862604 29.0 9.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1094834345243818 1101 2 0.12443167246311759 1101 3 0.14210177549206549 1101 4 0.15791699092029177 1101 5 0.021838971906859683 1101 6 0.1368205246280283 1101 7 0.0985544976006878 1101 8 0.051075908865207964 1101 9 0.20461742946395134 1101 10-11 0.15699738642477712 1101 12-13 0.19058485285722782 1101 14-15 0.2489740680288719 1101 16-17 0.2311486830533127 1101 18-19 0.17723182977022134 1101 20-21 0.18873460497731998 1101 22-23 0.12433852249095168 1101 24-25 0.09347203252534797 1101 26-27 0.2709348941806411 1101 28-29 0.27346953555530007 1101 30-31 0.202300613976945 1101 32-33 0.3890399479915132 1101 34-35 0.3740242831586258 1101 36-37 0.386706346812133 1101 38-39 0.542698651327207 1101 40-41 0.5294891793647309 1101 42-43 0.4823949118787496 1101 44-45 0.5350931205555867 1101 46-47 0.7278139060143687 1101 48-49 0.6686895121792276 1101 50-51 0.6115905845940262 1101 52-53 0.731284334793429 1101 54-55 0.7619221864269576 1101 56-57 0.4815947886832319 1101 58-59 0.5370869125709135 1101 60-61 0.5406473337167839 1101 62-63 0.49250275142382094 1101 64-65 0.4487113791211925 1101 66-67 0.5036515352950772 1101 68-69 0.5020420339518452 1101 70-71 0.4834379321592124 1101 72-73 0.515354504391567 1101 74-75 0.6933495796473501 1101 76-77 0.5896900650415517 1101 78-79 0.612366704461035 1101 80-81 0.6877308587863205 1101 82-83 0.9102843587513831 1101 84-85 0.9180697489393559 1101 86-87 1.0639151834614786 1101 88-89 1.0548448359371072 1101 90-91 1.087318762253453 1101 92-93 1.102558175410671 1101 94-95 1.0137858050755604 1101 96-97 0.9606861802146263 1101 98-99 1.0142662242038583 1101 100-101 1.940432547196 1102 1 0.1065349018431867 1102 2 0.042687934505501346 1102 3 0.10202840955164305 1102 4 0.10355394067573798 1102 5 0.0630572911598648 1102 6 0.07434831475696768 1102 7 0.1627200526622019 1102 8 0.057659390412560185 1102 9 0.10494257397262174 1102 10-11 0.19024132817666128 1102 12-13 0.30437570434677497 1102 14-15 0.32854091255236284 1102 16-17 0.35434834586641983 1102 18-19 0.33585742262630447 1102 20-21 0.31421491103927934 1102 22-23 0.2904503129163274 1102 24-25 0.16654525025175104 1102 26-27 0.22022624589250483 1102 28-29 0.2891413156056899 1102 30-31 0.3499000100048093 1102 32-33 0.4140214212388855 1102 34-35 0.24185720890340434 1102 36-37 0.36827037526922624 1102 38-39 0.4795344672453936 1102 40-41 0.4959923474394259 1102 42-43 0.4874480836588049 1102 44-45 0.41003198227191007 1102 46-47 0.4222591224068424 1102 48-49 0.39147602840866114 1102 50-51 0.24963619745900445 1102 52-53 0.28641809794742557 1102 54-55 0.24114119116980248 1102 56-57 0.10026002878995399 1102 58-59 0.08495216749903278 1102 60-61 0.05331777984098807 1102 62-63 -0.06959707071935384 1102 64-65 -0.10429088114262441 1102 66-67 -0.04438416575723636 1102 68-69 0.00923875101127436 1102 70-71 0.11847665307221433 1102 72-73 0.20682293480508918 1102 74-75 0.12884498501269803 1102 76-77 0.0983837437135513 1102 78-79 -0.017744826589801477 1102 80-81 -0.11341409452626294 1102 82-83 0.1221345329028587 1102 84-85 0.16416801483530818 1102 86-87 0.06116998189041212 1102 88-89 0.3123055592215316 1102 90-91 0.34697166354291653 1102 92-93 0.5233206439151914 1102 94-95 0.47496299493771943 1102 96-97 0.5395327972295938 1102 98-99 0.5409586430566797 1102 100-101 0.9202055018425099 1103 1 0.0254071117675565 1103 2 0.1223641934983064 1103 3 0.013543812613811212 1103 4 0.04512606758959237 1103 5 0.06579663814381576 1103 6 0.09036012069683608 1103 7 0.05636974829031516 1103 8 0.15686041420890717 1103 9 0.10139041569923535 1103 10-11 0.13437467534508585 1103 12-13 0.20277486962993407 1103 14-15 0.2630617574149312 1103 16-17 0.1558246648112558 1103 18-19 0.18694059455030043 1103 20-21 0.14688704256482055 1103 22-23 0.08688201370953408 1103 24-25 0.1733198305874808 1103 26-27 0.17647855174014637 1103 28-29 0.12807094113603767 1103 30-31 0.06513762760303621 1103 32-33 0.18833889034052476 1103 34-35 0.11740945941068759 1103 36-37 0.06714742747361413 1103 38-39 0.17586009904764666 1103 40-41 0.226244491877722 1103 42-43 0.27970850609008835 1103 44-45 0.31756979817303943 1103 46-47 0.2682832646315205 1103 48-49 0.3230136829004522 1103 50-51 0.1761246221039059 1103 52-53 0.11385076719664511 1103 54-55 0.08566076781617937 1103 56-57 0.1996299559251682 1103 58-59 0.008434343296855218 1103 60-61 0.03473495379413549 1103 62-63 -0.0192488877272865 1103 64-65 -0.009945972454190155 1103 66-67 -0.10632788001659321 1103 68-69 -0.17281137811618663 1103 70-71 -0.14043198924470346 1103 72-73 0.009513858988817958 1103 74-75 -0.026917328213645675 1103 76-77 -0.24223491520546148 1103 78-79 -0.2929316002477975 1103 80-81 -0.20872400044767758 1103 82-83 -0.08080254051533231 1103 84-85 -0.11191112185533925 1103 86-87 0.07549909552258427 1103 88-89 0.31199888148679733 1103 90-91 0.26390177330126363 1103 92-93 0.16618808073496538 1103 94-95 0.06525307056952556 1103 96-97 0.18580712392208554 1103 98-99 0.3337125168806985 1103 100-101 1.0545632079852894 1104 1 -0.09777081760514861 1104 2 -0.030682151784041878 1104 3 -0.0925010542731215 1104 4 -0.0765930096893328 1104 5 -0.08562255248642714 1104 6 -0.041921321646896104 1104 7 -0.08497336130638189 1104 8 -0.11300170185087666 1104 9 -0.07812909429200232 1104 10-11 -0.07460927843148824 1104 12-13 -0.08867572828099668 1104 14-15 -0.08144415271793548 1104 16-17 -0.003324832465317229 1104 18-19 -0.08350494738623837 1104 20-21 -0.09578236936488338 1104 22-23 -0.16915078229718716 1104 24-25 -0.14790824926886614 1104 26-27 -0.05957084460088424 1104 28-29 -0.05035920065657251 1104 30-31 -0.15818986572349303 1104 32-33 -0.23312991735540578 1104 34-35 -0.07960408986493661 1104 36-37 -0.15973039912416453 1104 38-39 -0.012888001225732637 1104 40-41 0.057684996233589914 1104 42-43 0.044368302701506934 1104 44-45 -0.07316091543441416 1104 46-47 -0.06755276453172598 1104 48-49 -0.08215426327952002 1104 50-51 0.06189604331783727 1104 52-53 -0.11701596798663871 1104 54-55 -0.17842353521119492 1104 56-57 -0.20605657826072132 1104 58-59 -0.3124340243292778 1104 60-61 -0.14984340461213463 1104 62-63 -0.3040787802849607 1104 64-65 0.0780921301413926 1104 66-67 0.03539379537558318 1104 68-69 0.04443078213451912 1104 70-71 -0.015267078028898595 1104 72-73 -0.023170980675402575 1104 74-75 -0.12145094389061128 1104 76-77 -0.14415119617876826 1104 78-79 -0.1775805445631029 1104 80-81 -0.20965828657582364 1104 82-83 -0.2132195215996866 1104 84-85 -0.05402469849163438 1104 86-87 0.10836626295833796 1104 88-89 0.2462617875569535 1104 90-91 0.14757527553494754 1104 92-93 0.22718177858589073 1104 94-95 0.29356595238295924 1104 96-97 0.28127698006018775 1104 98-99 0.21491367292614072 1104 100-101 0.7110794140041712 1105 1 -0.03637038212518107 1105 2 -0.0028728131580493255 1105 3 0.012362701427996114 1105 4 -0.04825231381742867 1105 5 -0.024074268868893967 1105 6 -0.009008982151726741 1105 7 0.041278643603966714 1105 8 -0.030653873426402356 1105 9 0.10313182630906681 1105 10-11 0.10996859273453907 1105 12-13 0.12047248723678905 1105 14-15 0.025836023982989786 1105 16-17 0.07908549539912713 1105 18-19 -0.029048670112736374 1105 20-21 0.023306841305277715 1105 22-23 0.03447888947994926 1105 24-25 -0.004716431991248271 1105 26-27 0.09799853201759845 1105 28-29 0.04452413372214892 1105 30-31 0.01146318398036783 1105 32-33 0.12403220484780064 1105 34-35 0.17191202558205276 1105 36-37 0.1023375930981203 1105 38-39 0.09241638672778407 1105 40-41 0.13193097073202154 1105 42-43 0.20116573283205952 1105 44-45 0.22713298874207055 1105 46-47 0.4330180188841979 1105 48-49 0.4587994468126837 1105 50-51 0.49040273232600384 1105 52-53 0.5763084645901273 1105 54-55 0.6256644657923829 1105 56-57 0.6966317937072759 1105 58-59 0.5814587275277816 1105 60-61 0.4631578229754254 1105 62-63 0.45036159211313276 1105 64-65 0.4165080045511189 1105 66-67 0.5199468011730275 1105 68-69 0.5158302319602299 1105 70-71 0.45941392841368867 1105 72-73 0.37823738484103586 1105 74-75 0.5341379397215427 1105 76-77 0.5620384885014751 1105 78-79 0.34338924961436845 1105 80-81 0.39983177175698614 1105 82-83 0.34947772125655874 1105 84-85 0.492906332592316 1105 86-87 0.6235598992160796 1105 88-89 0.6591872989771019 1105 90-91 0.732528367353126 1105 92-93 0.6167988071071413 1105 94-95 0.5873731315301001 1105 96-97 0.470740112110974 1105 98-99 0.7389501958963685 1105 100-101 0.7452661596426573 1106 1 -0.09921718309042404 1106 2 -0.10235486150020279 1106 3 -0.0605278287891835 1106 4 -0.056304228972017256 1106 5 -0.03429264848679736 1106 6 -0.14259451416874214 1106 7 -0.07446766451838727 1106 8 -0.1040919080987237 1106 9 -0.051057782660762996 1106 10-11 -0.16968119550744376 1106 12-13 0.001449596039968526 1106 14-15 0.03386160826179463 1106 16-17 -0.09060677776648873 1106 18-19 -0.13269288554151615 1106 20-21 -0.09384100267373441 1106 22-23 -0.15658894418890412 1106 24-25 -0.18377571979105056 1106 26-27 -0.18903867784281658 1106 28-29 -0.20226427713727446 1106 30-31 -0.07656504449609258 1106 32-33 -0.12136666903508342 1106 34-35 -0.13697225632941468 1106 36-37 -0.02786381032644414 1106 38-39 -0.19390389004638564 1106 40-41 -0.1893864840833075 1106 42-43 -0.05388823990826097 1106 44-45 -0.06814434092937915 1106 46-47 -0.16608250951092884 1106 48-49 -0.1782920913104249 1106 50-51 -0.007924201604602388 1106 52-53 -0.04073038956126851 1106 54-55 -0.02077642194699081 1106 56-57 -0.008985348692768014 1106 58-59 0.02200283230022393 1106 60-61 -0.2028057981977085 1106 62-63 -0.28591213345025324 1106 64-65 -0.17535031089499853 1106 66-67 -0.23644415789301831 1106 68-69 -0.26220337379211145 1106 70-71 -0.2473306643132105 1106 72-73 -0.1822361931805716 1106 74-75 -0.13630781867434294 1106 76-77 -0.2824881334582514 1106 78-79 -0.25122858605429954 1106 80-81 -0.303389038745145 1106 82-83 -0.1697197092054843 1106 84-85 -0.1763336494537988 1106 86-87 -0.018844031972566455 1106 88-89 0.06411661668522939 1106 90-91 0.055947737100837 1106 92-93 0.022199191960108777 1106 94-95 -0.03237313659598584 1106 96-97 -0.050663556879921856 1106 98-99 -0.077442496341245 1106 100-101 0.4555953067000402 1107 1 -0.040085676483595734 1107 2 -0.018717174983549967 1107 3 -0.10708865951067992 1107 4 -0.05658081507945667 1107 5 -0.08113136689740941 1107 6 -0.12245123605718078 1107 7 -0.1849919069110726 1107 8 0.009157846336201203 1107 9 -0.10623443332664806 1107 10-11 -0.03722042084073962 1107 12-13 -0.1267672630872525 1107 14-15 -0.07083794763853746 1107 16-17 -0.07090256654234395 1107 18-19 -0.01309857119738922 1107 20-21 -0.0891066077703826 1107 22-23 -0.1405505293071414 1107 24-25 -0.07747847237598648 1107 26-27 -0.11968812805471174 1107 28-29 -0.04892016670761734 1107 30-31 -0.09573972890591165 1107 32-33 -0.08501002177066397 1107 34-35 -0.010701044886602062 1107 36-37 -0.017973308054749282 1107 38-39 -0.06043254566869649 1107 40-41 -0.058343348798644 1107 42-43 -0.12946306176511513 1107 44-45 -0.2042717796595923 1107 46-47 -0.29297283997384227 1107 48-49 -0.2562727784414278 1107 50-51 -0.28990970372010594 1107 52-53 -0.2122975112608927 1107 54-55 -0.10897636918954845 1107 56-57 -0.06612999086193128 1107 58-59 0.0016088912497309593 1107 60-61 -0.08285571819381943 1107 62-63 -0.11692301120223192 1107 64-65 -0.05087636682482355 1107 66-67 -0.24452980023225734 1107 68-69 -0.14754465718636567 1107 70-71 -0.0576042003809043 1107 72-73 -0.023707665980985837 1107 74-75 0.005659274737570996 1107 76-77 0.018249689811252523 1107 78-79 -0.14371338358025554 1107 80-81 -0.15148610463294432 1107 82-83 -0.12835500948905576 1107 84-85 -0.3200016931938876 1107 86-87 -0.11974839261829118 1107 88-89 0.08907422850844782 1107 90-91 0.19168144737222548 1107 92-93 0.08631369557156532 1107 94-95 -0.004367437403658414 1107 96-97 0.15184856084554 1107 98-99 0.2526725678180526 1107 100-101 0.603253999877321 1108 1 -0.11322724390580063 1108 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76.0 6 254.0 7 585.0 8 1144.0 9 1856.0 10 3499.0 11 5636.0 12 8087.0 13 11629.0 14 13640.0 15 18465.0 16 24961.0 17 34873.0 18 29974.0 19 16517.0 20 13360.0 21 13300.0 22 14136.0 23 15534.0 24 17806.0 25 20681.0 26 24820.0 27 29811.0 28 36332.0 29 44658.0 30 53369.0 31 64071.0 32 78629.0 33 98064.0 34 127809.0 35 177656.0 36 247393.0 37 424018.0 38 458532.0 39 55130.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.29733973682036 24.076851027098503 16.2910586874076 19.33475054867354 2 13.951419511322745 24.620703629014738 40.69561566280986 20.732261196852654 3 17.92049858417061 30.62682981826797 32.705636011784016 18.747035585777397 4 10.422114289974482 20.61233534780097 42.32335064223448 26.642199719990067 5 9.5652985270892 41.94257921074887 36.54408647101952 11.94803579114241 6 23.661479789841614 42.970090346883914 19.36075223054304 14.007677632731433 7 23.068482599546 37.14719182784466 24.258867857905706 15.52545771470363 8 23.848686693033276 35.526729239724084 21.6395263157654 18.985057751477232 9 25.17958003998443 18.38762394510305 24.609909387833884 31.822886627078635 10-11 22.031549371532748 29.316838879027202 29.46583142414004 19.18578032530001 12-13 24.27462470573115 28.615190333116686 29.14042621701639 17.969758744135778 14-15 21.611149378918068 29.118515550603608 29.802510673059068 19.467824397419257 16-17 17.348882225194252 32.2707962464575 30.77695365886234 19.60336786948591 18-19 17.1393954030212 31.094875520788314 34.259695044112355 17.506034032078137 20-21 18.59831747052509 29.283838688298445 33.99637112247758 18.12147271869887 22-23 18.219009664962304 29.389196885586987 33.79157783905695 18.600215610393754 24-25 18.358968893832692 30.22307488596296 34.04970199204062 17.368254228163728 26-27 18.02314771723368 29.23352373442581 35.394455461032194 17.34887308730832 28-29 16.840639952139433 30.04771420508418 35.32406278772255 17.787583055053833 30-31 17.70132060222718 29.650339767036492 34.641853023599595 18.006486607136733 32-33 17.715796777370148 30.022672480310803 34.53768402237335 17.723846719945698 34-35 18.416391756915743 29.524964183970887 34.565555683633306 17.493088375480063 36-37 18.501119857712197 28.845279397179862 34.80836160425107 17.845239140856876 38-39 17.496699629205743 30.663806802743494 34.25224530423335 17.587248263817415 40-41 17.490147753395025 30.309103587930803 34.61358220619424 17.587166452479934 42-43 17.40568251363271 30.343834246754543 33.47924288039168 18.77124035922107 44-45 18.01467906105786 29.77608634451221 33.44148352144697 18.767751072982968 46-47 18.71885176918292 30.578735043360535 32.75135844707088 17.951054740385672 48-49 18.177015654865713 30.57791779811915 32.5651577126982 18.679908834316937 50-51 17.202142023396135 30.76071286128807 33.00615090763393 19.030994207681864 52-53 17.85529862313965 31.792767949885448 31.639407396159168 18.71252603081573 54-55 17.765788750296725 30.812824656242295 31.923762469978517 19.49762412348246 56-57 18.38734951524252 30.768206934751095 31.484468870734844 19.359974679271534 58-59 18.748453467731274 30.29417159155723 31.82853742528048 19.12883751543102 60-61 18.29728523220106 31.37714754077366 32.00390333059207 18.32166389643321 62-63 17.693087706411276 30.83548798889108 32.49864728947914 18.972777015218508 64-65 17.44831913997341 31.262981104131953 31.920057666861357 19.36864208903328 66-67 17.869665484776302 30.790256539732376 30.729995682258103 20.61008229323322 68-69 17.564947175283564 30.642246188769377 31.366289101690526 20.42651753425654 70-71 18.470524469367266 30.588641566478604 31.366643720798333 19.574190243355797 72-73 18.510820083326056 29.66632530641237 31.86711557293408 19.95573903732749 74-75 17.48406362930746 31.6746716332373 30.772552074209493 20.068712663245748 76-77 17.720278513286424 30.360095419895384 31.47652467757099 20.4431013892472 78-79 17.88417402858382 30.290148299850106 31.325003248577488 20.50067442298858 80-81 18.13972230442413 30.309245862169433 30.86087275099012 20.69015908241632 82-83 18.061098149382236 30.221679867505262 31.38439344826151 20.332828534850993 84-85 17.575906155964894 30.295844537313542 30.996212410541585 21.132036896179983 86-87 17.37108653718863 30.437461321724008 31.232830968089477 20.95862117299788 88-89 18.016803421807737 29.83869302436354 30.68655042517916 21.457953128649564 90-91 17.884393241216753 29.25940186983616 31.2512885596088 21.60491632933829 92-93 18.47502985568191 30.059400343311754 30.96890663941883 20.496663161587502 94-95 18.07201913509254 28.965428445909495 31.10152333833177 21.861029080666196 96-97 17.915604584991158 28.938377232201052 31.21596212136112 21.930056061446678 98-99 17.875684875273688 29.228457330881145 30.414362404503954 22.481495389341216 100-101 17.719355378720035 28.447339798329445 31.318785110079045 22.51451971287148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6690.0 1 4106.5 2 1756.5 3 2039.0 4 1703.5 5 1681.0 6 3082.5 7 5919.5 8 8566.0 9 8996.0 10 7042.0 11 5292.5 12 5040.5 13 5139.5 14 5343.5 15 5471.0 16 5694.0 17 6063.5 18 6545.0 19 7157.0 20 7864.5 21 8896.0 22 10179.0 23 11431.0 24 12874.0 25 15955.5 26 22800.5 27 29275.5 28 30514.0 29 33683.5 30 42059.5 31 50467.0 32 62091.5 33 77410.5 34 93204.5 35 103194.5 36 113124.5 37 123180.0 38 118993.5 39 109935.0 40 100356.5 41 91143.5 42 83313.5 43 77815.5 44 74771.5 45 71516.5 46 67486.0 47 72010.0 48 94031.0 49 85655.0 50 51517.5 51 33807.5 52 24688.0 53 18762.5 54 13830.5 55 10572.5 56 8535.0 57 6684.0 58 5286.0 59 4269.5 60 3454.0 61 2757.5 62 2042.0 63 1351.5 64 829.5 65 537.0 66 390.5 67 341.0 68 285.0 69 220.0 70 158.0 71 109.5 72 108.5 73 123.0 74 131.5 75 111.5 76 69.0 77 50.0 78 38.5 79 24.5 80 14.5 81 10.0 82 11.0 83 7.0 84 2.5 85 1.0 86 0.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.382875393749677 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.286915504418092E-4 16-17 0.001966747333799559 18-19 9.147662017672369E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.860746513254277E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00818715750581677 36-37 0.0164200533217219 38-39 0.010657026250588309 40-41 0.007981335110419142 42-43 0.0029729901557435197 44-45 0.0018066632484902928 46-47 0.001417887612739217 48-49 9.147662017672369E-5 50-51 3.201681706185329E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.6590648070689474E-4 60-61 5.94598031148704E-4 62-63 0.0 64-65 0.0 66-67 0.0010519811320323224 68-69 7.546821164579703E-4 70-71 0.0021039622640646447 72-73 0.0 74-75 0.0 76-77 0.09653070344148766 78-79 0.03563014355883387 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14993018046965012 88-89 0.3271203937519639 90-91 0.2793009905545816 92-93 0.0 94-95 6.860746513254277E-5 96-97 0.0 98-99 1.8295324035344737E-4 100-101 5.94598031148704E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2186351.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.091168051267168 #Duplication Level Percentage of deduplicated Percentage of total 1 70.33413342824171 15.537631613035352 2 12.6777785773314 5.601338741371655 3 5.173995103433355 3.4289878597913908 4 2.9009980845543586 2.5634574480917793 5 1.7876886784185455 1.97460655091459 6 1.1286766246947457 1.496027099500118 7 0.8421485081923155 1.302283095302027 8 0.6247158347246556 1.104056199135201 9 0.46362318237014777 0.9217779870781997 >10 3.053840633964808 13.20007163254344 >50 0.4237772699604292 6.6072635866101415 >100 0.5019885696209276 23.003459578146177 >500 0.06155680582372379 9.327552672981096 >1k 0.02321334091669045 9.12622903391583 >5k 8.290478898818019E-4 1.2553767422996285 >10k+ 0.0010363098623522523 3.5498801592834086 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19944 0.9122048564022887 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18961 0.8672440975854288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16678 0.7628235356536988 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11800 0.5397120590426697 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10148 0.46415237077669597 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8174 0.37386494666226966 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7516 0.3437691386241276 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6693 0.3061265094214058 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5035 0.23029239129490187 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4785 0.2188578137728114 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4724 0.21606777685742135 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4673 0.2137351230429149 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3971 0.18162682936088487 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3936 0.1800259885077922 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3743 0.1711984946607384 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3624 0.1657556357602233 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3561 0.1628741222246565 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3476 0.15898636586714576 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3011 0.1377180516760575 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2985 0.1365288556137601 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2688 0.12294457751751663 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2658 0.12157242821486577 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2636 0.12056618539292181 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2466 0.1127906726779003 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 2406 0.11004637407259858 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2398 0.1096804675918917 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2386 0.10913160787083134 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2385 0.10908586956074298 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2362 0.10803388842871067 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2343 0.10716486053703181 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2331 0.10661600081597145 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 2294 0.10492368334270206 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.573831008836184E-5 0.0 2 0.0 0.0 0.0 4.573831008836184E-5 0.0 3 0.0 0.0 0.0 4.573831008836184E-5 0.0 4 0.0 0.0 0.0 4.573831008836184E-5 0.0 5 0.0 0.0 0.0 4.573831008836184E-5 0.0 6 0.0 0.0 0.0 9.147662017672369E-5 0.0 7 0.0 0.0 0.0 1.8295324035344737E-4 0.0 8 0.0 0.0 0.0 1.8295324035344737E-4 0.0 9 0.0 0.0 0.0 1.8295324035344737E-4 0.0 10-11 0.0 0.0 0.0 2.286915504418092E-4 0.0 12-13 0.0 0.0 0.0 2.7442986053017107E-4 0.0 14-15 0.0 0.0 0.0 2.97299015574352E-4 0.0 16-17 0.0 0.0 0.0 4.3451394583943746E-4 0.0 18-19 0.0 0.0 0.0 5.031214109719802E-4 0.0 20-21 0.0 0.0 0.0 8.004204265463321E-4 0.0 22-23 0.0 0.0 0.0 0.0017151866283135692 0.0 24-25 0.0 0.0 0.0 0.0033617657914945956 0.0 26-27 0.0 0.0 0.0 0.0063804942573264765 0.0 28-29 0.0 0.0 0.0 0.014750605003496694 0.0 30-31 0.0 0.0 0.0 0.03915199343563773 0.0 32-33 0.0 0.0 0.0 0.08399840647727652 0.0 34-35 0.0 0.0 0.0 0.15230857259424493 0.0 36-37 4.573831008836184E-5 0.0 0.0 0.25682061114615173 0.0 38-39 4.573831008836184E-5 0.0 0.0 0.41713338800586 0.0 40-41 4.573831008836184E-5 0.0 0.0 0.5993090770878051 0.0 42-43 4.573831008836184E-5 0.0 0.0 0.7753329634628657 0.0 44-45 4.573831008836184E-5 0.0 0.0 0.9864381336756998 0.0 46-47 4.573831008836184E-5 2.286915504418092E-5 0.0 1.2376786709910714 0.0 48-49 4.573831008836184E-5 4.573831008836184E-5 0.0 1.4975408797580991 0.0 50-51 4.573831008836184E-5 4.573831008836184E-5 0.0 1.7855550183845137 0.0 52-53 4.573831008836184E-5 4.573831008836184E-5 0.0 2.0633466447061792 0.0 54-55 4.573831008836184E-5 4.573831008836184E-5 0.0 2.3613317349318566 0.0 56-57 4.573831008836184E-5 4.573831008836184E-5 0.0 2.722069786598767 0.0 58-59 4.573831008836184E-5 4.573831008836184E-5 0.0 3.1079410396592313 0.0 60-61 4.573831008836184E-5 4.573831008836184E-5 0.0 3.5322553423489644 0.0 62-63 4.573831008836184E-5 4.573831008836184E-5 0.0 3.954031168828793 0.0 64-65 4.573831008836184E-5 4.573831008836184E-5 0.0 4.38339955478329 0.0 66-67 4.573831008836184E-5 4.573831008836184E-5 0.0 4.848215131056266 0.0 68-69 4.573831008836184E-5 4.573831008836184E-5 0.0 5.36535533407033 0.0 70-71 4.573831008836184E-5 4.573831008836184E-5 0.0 5.962812009599556 0.0 72-73 4.573831008836184E-5 4.573831008836184E-5 0.0 6.526994064539499 0.0 74-75 4.573831008836184E-5 4.573831008836184E-5 0.0 7.108213639987358 0.0 76-77 4.573831008836184E-5 4.573831008836184E-5 0.0 7.704664072694641 0.0 78-79 4.573831008836184E-5 4.573831008836184E-5 0.0 8.410268982427798 0.0 80-81 4.573831008836184E-5 4.573831008836184E-5 0.0 9.171720368778846 0.0 82-83 4.573831008836184E-5 4.573831008836184E-5 0.0 9.902321264975296 0.0 84-85 4.573831008836184E-5 4.573831008836184E-5 0.0 10.616707930245418 0.0 86-87 4.573831008836184E-5 4.573831008836184E-5 0.0 11.355358769017418 0.0 88-89 4.573831008836184E-5 4.573831008836184E-5 0.0 12.134465143062574 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACGC 40 5.416525E-4 47.45139 3 CCGATAC 85 1.644356E-5 33.4951 9 CATTCCG 650 0.0 29.930878 9 GTACGGG 65 0.005756652 29.32223 1 CATGGGT 1945 0.0 27.080229 4 CATATAG 1135 0.0 26.756733 3 GACATAT 1180 0.0 26.650925 1 ATGGGTA 1170 0.0 25.956318 5 TAGGCAT 815 0.0 25.61793 5 GTATGGT 360 0.0 25.147884 1 CCCGATA 115 1.2912141E-4 24.757248 8 CATGGGG 4855 0.0 23.163708 4 GAGTACT 15870 0.0 23.053736 12-13 GTACATG 17830 0.0 22.90234 1 ACATGGG 16365 0.0 22.848577 3 AGGCTTA 1230 0.0 22.761236 6 GGACAAT 880 0.0 22.647255 6 GACAATG 865 0.0 22.491411 7 GGCATTC 845 0.0 22.462196 7 TTAGGCA 965 0.0 22.12759 4 >>END_MODULE