##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938862_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2181692 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64741677560352 33.0 31.0 34.0 30.0 34.0 2 31.693545651723525 33.0 31.0 34.0 30.0 34.0 3 31.668901476468722 33.0 31.0 34.0 28.0 34.0 4 35.26145899604527 37.0 35.0 37.0 33.0 37.0 5 35.325232892635626 37.0 35.0 37.0 33.0 37.0 6 35.42164476012196 37.0 35.0 37.0 33.0 37.0 7 35.36004807278021 37.0 35.0 37.0 33.0 37.0 8 35.34400089471841 37.0 35.0 37.0 33.0 37.0 9 36.887218727483074 39.0 37.0 39.0 33.0 39.0 10-11 36.78587444973901 39.0 37.0 39.0 32.5 39.0 12-13 36.70760790249036 39.0 37.0 39.0 32.0 39.0 14-15 37.7959973268454 40.0 37.5 41.0 32.0 41.0 16-17 37.71563882527873 40.0 37.0 41.0 32.0 41.0 18-19 37.79792220900109 40.0 37.5 41.0 32.0 41.0 20-21 37.753061156203536 40.0 37.5 41.0 32.0 41.0 22-23 37.743517187577346 40.0 37.5 41.0 32.0 41.0 24-25 37.67691589830278 40.0 37.0 41.0 32.0 41.0 26-27 37.53574908832228 40.0 37.0 41.0 32.0 41.0 28-29 37.392970455958036 40.0 37.0 41.0 32.0 41.0 30-31 37.126114960315206 39.5 36.5 41.0 31.0 41.0 32-33 36.86766578417118 39.0 35.5 41.0 31.0 41.0 34-35 36.55305423496992 39.0 35.0 41.0 30.0 41.0 36-37 36.30505382977982 39.0 35.0 41.0 30.0 41.0 38-39 36.129308811692944 39.0 35.0 41.0 30.0 41.0 40-41 36.02090304222594 39.0 35.0 41.0 30.0 41.0 42-43 35.8858640449706 39.0 35.0 41.0 29.5 41.0 44-45 35.56896207163981 39.0 35.0 41.0 27.0 41.0 46-47 35.10633123282297 39.0 35.0 41.0 24.5 41.0 48-49 34.70958916290659 39.0 35.0 41.0 22.5 41.0 50-51 33.58038141955876 38.0 34.0 40.0 17.5 40.5 52-53 33.70846411867487 38.0 33.5 39.5 19.0 40.5 54-55 34.260885817063084 39.0 34.5 40.0 19.0 41.0 56-57 34.19833299109132 39.0 34.0 41.0 16.5 41.0 58-59 34.055712263692584 39.0 34.0 40.5 14.5 41.0 60-61 33.769327201089794 38.0 34.0 40.0 11.5 41.0 62-63 33.43458861287478 38.0 33.5 40.0 8.0 41.0 64-65 33.10451131507105 38.0 33.0 40.0 7.0 41.0 66-67 32.715938592615274 37.0 33.0 40.0 2.0 41.0 68-69 32.287233486669976 36.5 32.0 39.0 2.0 41.0 70-71 31.86114744886079 36.0 31.5 39.0 2.0 40.5 72-73 31.41927297712051 36.0 31.0 38.5 2.0 40.0 74-75 30.920651035984914 35.0 31.0 37.5 2.0 40.0 76-77 30.444186209602456 35.0 30.5 37.0 2.0 39.0 78-79 29.96784743217649 35.0 30.0 36.5 2.0 39.0 80-81 29.43864143059607 34.5 29.0 36.0 2.0 38.5 82-83 28.974907548819907 34.0 29.0 36.0 2.0 37.0 84-85 28.554135964196597 34.0 29.0 35.0 2.0 37.0 86-87 28.146699671631012 34.0 28.5 35.0 2.0 36.5 88-89 27.649514230239646 34.0 26.5 35.0 2.0 36.0 90-91 27.151656374960353 34.0 26.0 35.0 2.0 36.0 92-93 26.675080625496175 34.0 25.0 35.0 2.0 35.0 94-95 26.14293630814982 33.5 24.5 35.0 2.0 35.0 96-97 25.53921085102755 33.0 20.0 35.0 2.0 35.0 98-99 24.840814835457984 33.0 8.5 35.0 2.0 35.0 100-101 22.46203772118154 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12362659190667458 1101 2 0.2304375623991568 1101 3 0.28290787791265615 1101 4 0.12919209817302857 1101 5 0.10400163827051045 1101 6 0.255525290621776 1101 7 0.2179050527013544 1101 8 0.23699067098428372 1101 9 0.3248276048483021 1101 10-11 0.25113323284003286 1101 12-13 0.23110076410475244 1101 14-15 0.1971307398666582 1101 16-17 0.419884241464338 1101 18-19 0.3724913838808064 1101 20-21 0.5657670854784769 1101 22-23 0.4760003848304706 1101 24-25 0.48265910615413077 1101 26-27 0.4828961553048998 1101 28-29 0.34282305092330034 1101 30-31 0.4432565816388916 1101 32-33 0.4901576379577932 1101 34-35 0.4664113238770611 1101 36-37 0.3678542345617828 1101 38-39 0.4413675723241681 1101 40-41 0.5536289184736702 1101 42-43 0.5877281935880632 1101 44-45 0.5940445123710205 1101 46-47 0.698589032089096 1101 48-49 0.7094640978464 1101 50-51 0.6024227114807559 1101 52-53 0.5247476710480825 1101 54-55 0.6845503450501056 1101 56-57 0.7235516921766845 1101 58-59 0.7990653780013801 1101 60-61 0.566687315446238 1101 62-63 0.4123987483837155 1101 64-65 0.46362165628227103 1101 66-67 0.34745028628677943 1101 68-69 0.5121997821632007 1101 70-71 0.304199260074121 1101 72-73 0.24462019635773302 1101 74-75 0.2548311239487653 1101 76-77 0.20142046443619321 1101 78-79 0.0020044301114019447 1101 80-81 -0.042952882897495215 1101 82-83 0.1147372220391496 1101 84-85 -0.02530754068397556 1101 86-87 -0.11345143463676521 1101 88-89 0.009119248372655875 1101 90-91 -0.043498022741108144 1101 92-93 -0.1543328893054472 1101 94-95 -0.17351544943548092 1101 96-97 -0.14778305892443555 1101 98-99 -0.035701510289186444 1101 100-101 0.09559992859020738 1102 1 0.1437782905421443 1102 2 0.20464176627866237 1102 3 0.16417549319136526 1102 4 0.29633811666952425 1102 5 0.3212259569061189 1102 6 0.2766086617986758 1102 7 0.21311198492172423 1102 8 0.3208763401666417 1102 9 0.45073314730878167 1102 10-11 0.3680006956632411 1102 12-13 0.40822081470700056 1102 14-15 0.4864661978458784 1102 16-17 0.5960636091524236 1102 18-19 0.6106121084589375 1102 20-21 0.5745150311134921 1102 22-23 0.4336444246491169 1102 24-25 0.3974754927784119 1102 26-27 0.438928689958793 1102 28-29 0.422424513126586 1102 30-31 0.5413362910003059 1102 32-33 0.41022008885126127 1102 34-35 0.5025012455949494 1102 36-37 0.4366637480737481 1102 38-39 0.44750814134722106 1102 40-41 0.42511194473350855 1102 42-43 0.41906640513720106 1102 44-45 0.398192797877293 1102 46-47 0.3599204317964322 1102 48-49 0.3296678764363179 1102 50-51 0.2532588290192592 1102 52-53 0.3004448035870624 1102 54-55 0.2455156533129852 1102 56-57 0.2705087991982893 1102 58-59 0.1864706339320108 1102 60-61 0.17633949703768792 1102 62-63 0.0719321282105696 1102 64-65 0.1297519805115286 1102 66-67 0.1612468789242243 1102 68-69 0.04793537097302902 1102 70-71 0.08778880335635009 1102 72-73 -0.1531156771798834 1102 74-75 -0.15546858807972086 1102 76-77 -0.2501330139280995 1102 78-79 -0.18889585930176978 1102 80-81 -0.23282170989113027 1102 82-83 -0.25323829137188625 1102 84-85 -0.3441220099009392 1102 86-87 -0.4395519165114976 1102 88-89 -0.377101143542518 1102 90-91 -0.4749935103464402 1102 92-93 -0.7091776766246092 1102 94-95 -0.6991141436522632 1102 96-97 -0.6937832643609347 1102 98-99 -0.717269080876445 1102 100-101 -0.33209881320134116 1103 1 -0.011228657985302704 1103 2 0.2070831046719519 1103 3 0.20110246238622764 1103 4 0.2113611893439895 1103 5 0.23616172756908327 1103 6 0.32074205570891223 1103 7 0.3172695205106564 1103 8 0.24852747063861358 1103 9 0.3862347775402242 1103 10-11 0.25839324292213917 1103 12-13 0.34841136470968337 1103 14-15 0.4007864002440158 1103 16-17 0.440307689541541 1103 18-19 0.3807064926234247 1103 20-21 0.4706271963815496 1103 22-23 0.3669300982108581 1103 24-25 0.402790893565907 1103 26-27 0.40873332943711915 1103 28-29 0.4961528435201572 1103 30-31 0.6359578635055243 1103 32-33 0.5257411393260796 1103 34-35 0.5300374581129859 1103 36-37 0.6342934539194118 1103 38-39 0.5705300381170488 1103 40-41 0.5649973831143242 1103 42-43 0.84563093735877 1103 44-45 0.8781454053567614 1103 46-47 0.7707759469673832 1103 48-49 0.77889373921181 1103 50-51 0.7941959003040324 1103 52-53 0.6131532594104598 1103 54-55 0.6836924666113973 1103 56-57 0.6705054585600934 1103 58-59 0.6463549142249221 1103 60-61 0.5886392814551726 1103 62-63 0.47379241826705254 1103 64-65 0.41205082955031 1103 66-67 0.44270990028649493 1103 68-69 0.40940200022455286 1103 70-71 0.3663652906084707 1103 72-73 0.3600638086290857 1103 74-75 0.2121991564995369 1103 76-77 0.2034494864727776 1103 78-79 0.17048977506315666 1103 80-81 0.39314462538422745 1103 82-83 0.4262524171997448 1103 84-85 0.2893799143066609 1103 86-87 0.20174282576940072 1103 88-89 0.28559105450833044 1103 90-91 0.15193982833191555 1103 92-93 0.04202495312577881 1103 94-95 0.20070768897730673 1103 96-97 0.19198361254322904 1103 98-99 0.026252976244013126 1103 100-101 0.4416166000578734 1104 1 0.009901223541682214 1104 2 0.10315813758112213 1104 3 0.08676283974471843 1104 4 0.1989346864918673 1104 5 0.18430565034222468 1104 6 0.12777364851277184 1104 7 0.1312624998875762 1104 8 0.22270254742667817 1104 9 0.21036816937874647 1104 10-11 0.19828683660357171 1104 12-13 0.28508753847010126 1104 14-15 0.30681097242479183 1104 16-17 0.32316091464026897 1104 18-19 0.30693839257954636 1104 20-21 0.2859683547843517 1104 22-23 0.13227235358601774 1104 24-25 0.13110265310956493 1104 26-27 0.16998607186800996 1104 28-29 0.16980356752191028 1104 30-31 0.299970588384852 1104 32-33 0.23485917355161234 1104 34-35 0.22135163099583366 1104 36-37 0.13740775843894681 1104 38-39 0.1916489499204701 1104 40-41 0.1834440702577993 1104 42-43 0.16258683907004468 1104 44-45 0.319817191227763 1104 46-47 0.49118511765628625 1104 48-49 0.5674545114803422 1104 50-51 0.5872520652886664 1104 52-53 0.734896348484618 1104 54-55 0.5803796100953846 1104 56-57 0.5074328389901055 1104 58-59 0.5561333258089505 1104 60-61 0.5057388865451173 1104 62-63 0.5154674950551197 1104 64-65 0.472345915114154 1104 66-67 0.42600301130009655 1104 68-69 0.41721354037373715 1104 70-71 0.4202606394456865 1104 72-73 0.2557439314899881 1104 74-75 0.17164299151489715 1104 76-77 0.1756895040243336 1104 78-79 0.10569052100435883 1104 80-81 0.01963321687172126 1104 82-83 0.12830814339544716 1104 84-85 0.15117346410920973 1104 86-87 0.18752606403179684 1104 88-89 0.25244274384576215 1104 90-91 0.105740837546481 1104 92-93 0.20700520762336794 1104 94-95 0.17737728090358829 1104 96-97 0.27189695172708284 1104 98-99 0.5770416993681877 1104 100-101 0.9611229624975408 1105 1 -0.10148379406357932 1105 2 -0.0384552213343099 1105 3 0.042936910460944944 1105 4 0.11744066384748919 1105 5 3.7767378780273475E-4 1105 6 0.1164830878853067 1105 7 0.11511330846460766 1105 8 0.24757509375908882 1105 9 0.25562529054933947 1105 10-11 0.19883333768940759 1105 12-13 0.21328631980641433 1105 14-15 0.35468400369259 1105 16-17 0.3760218225236329 1105 18-19 0.1776584161937862 1105 20-21 0.2908988825205441 1105 22-23 0.1736206847757984 1105 24-25 0.14603423505310786 1105 26-27 0.21561806916923842 1105 28-29 0.31924167500889666 1105 30-31 0.28081964697769735 1105 32-33 0.2943105640511021 1105 34-35 0.22193819196063913 1105 36-37 0.3130912010584339 1105 38-39 0.43616749342761096 1105 40-41 0.41446538633024943 1105 42-43 0.16835904594769602 1105 44-45 0.2825895456944352 1105 46-47 0.3366214540749368 1105 48-49 0.26882856532925814 1105 50-51 0.3344510363823048 1105 52-53 0.38745512054390474 1105 54-55 0.3550501703011619 1105 56-57 0.1108027181779434 1105 58-59 0.1338893400618275 1105 60-61 0.27598767947771563 1105 62-63 0.25689489179550407 1105 64-65 0.18135517105461219 1105 66-67 0.05511389282827395 1105 68-69 0.17196400949600132 1105 70-71 0.22292694065380658 1105 72-73 0.28019458404433095 1105 74-75 0.199493884428513 1105 76-77 0.13321949622320162 1105 78-79 0.08920566996385304 1105 80-81 0.18065959313940638 1105 82-83 0.15469157968969682 1105 84-85 0.014019191406848819 1105 86-87 0.09126112064272718 1105 88-89 0.1344257078551223 1105 90-91 0.14936815416711724 1105 92-93 0.14720550701825275 1105 94-95 0.18999761867162235 1105 96-97 0.2068669068178508 1105 98-99 0.2486137333558318 1105 100-101 0.5120415987749247 1106 1 0.1677924727290936 1106 2 0.164417180926268 1106 3 0.22741207328845192 1106 4 0.21944258172536735 1106 5 0.2365513357491551 1106 6 0.21129746003373384 1106 7 0.18908077321154337 1106 8 0.22350024910637956 1106 9 0.12935028190417341 1106 10-11 0.24361386493414727 1106 12-13 0.28387379048847095 1106 14-15 0.25672835032653296 1106 16-17 0.40709720755028656 1106 18-19 0.3281525599626889 1106 20-21 0.29786467873008604 1106 22-23 0.27935289964679555 1106 24-25 0.13482106693304274 1106 26-27 0.17192769117725248 1106 28-29 0.04830504591225093 1106 30-31 0.030239215054592705 1106 32-33 0.15604125671893598 1106 34-35 0.2844392649421863 1106 36-37 0.3100852177045681 1106 38-39 0.25378454242996185 1106 40-41 0.47441603326658566 1106 42-43 0.5039809297027134 1106 44-45 0.5411037053720733 1106 46-47 0.5784436996597648 1106 48-49 0.4583610478529394 1106 50-51 0.3688267082731329 1106 52-53 0.20806262021485367 1106 54-55 0.15432638804325194 1106 56-57 0.34338792108795246 1106 58-59 0.43454714928284943 1106 60-61 0.5227223411553865 1106 62-63 0.4570496872668315 1106 64-65 0.38118480202253835 1106 66-67 0.48680155178282547 1106 68-69 0.5134280223080196 1106 70-71 0.471516327578712 1106 72-73 0.39446119195929796 1106 74-75 0.31242182012646325 1106 76-77 0.26958972840414575 1106 78-79 0.43819036372878983 1106 80-81 0.3302673519609485 1106 82-83 0.2749470596619332 1106 84-85 0.5574577763955766 1106 86-87 0.5003681383916856 1106 88-89 0.6392978615927376 1106 90-91 0.6942733516488566 1106 92-93 0.7657902014371949 1106 94-95 0.869242149735765 1106 96-97 0.8392595453060459 1106 98-99 1.031530763473711 1106 100-101 1.3666540296646943 1107 1 0.013081010875438892 1107 2 0.06585947794084035 1107 3 0.06941398170620516 1107 4 0.08980545165292853 1107 5 0.03603582874441713 1107 6 0.010760349061619934 1107 7 -0.019398723411981678 1107 8 0.0175502338220781 1107 9 0.07171331981772511 1107 10-11 0.11177656259530977 1107 12-13 2.3964378258511942E-4 1107 14-15 0.23126363283575557 1107 16-17 0.24411707956596018 1107 18-19 0.1985007916880548 1107 20-21 0.06480743871566119 1107 22-23 0.1774973611366093 1107 24-25 0.09147341306332635 1107 26-27 -0.015757583033625622 1107 28-29 -0.08452422176979013 1107 30-31 -0.3008626841966162 1107 32-33 -0.20251342978730236 1107 34-35 -0.13748339123934983 1107 36-37 -0.022444562455881112 1107 38-39 -0.12196377256153568 1107 40-41 -0.0758800218489668 1107 42-43 0.02149205574590951 1107 44-45 0.26808659624135345 1107 46-47 0.14464380812379574 1107 48-49 0.08577296928557132 1107 50-51 0.2006296259797864 1107 52-53 0.13504434002795307 1107 54-55 -0.0604612651480636 1107 56-57 0.09365827353951772 1107 58-59 0.14555875202667323 1107 60-61 0.15925204539909288 1107 62-63 0.016522078002239482 1107 64-65 -0.0750264799326601 1107 66-67 -0.290570271071104 1107 68-69 -0.040608244897924806 1107 70-71 0.01735611013377536 1107 72-73 -0.014365421867161388 1107 74-75 0.08958204524384428 1107 76-77 -0.04045688366976563 1107 78-79 0.07525946048508203 1107 80-81 0.0027565570439804787 1107 82-83 -0.04705048354297148 1107 84-85 0.15034039981984648 1107 86-87 0.023201815705945705 1107 88-89 0.1602606757134133 1107 90-91 0.1796970337159891 1107 92-93 0.3028467598161626 1107 94-95 0.2993325189294005 1107 96-97 0.2922141806088696 1107 98-99 0.5257173781401008 1107 100-101 0.9223386593908636 1108 1 0.1132763558049028 1108 2 0.19033074389823668 1108 3 0.11702421438169708 1108 4 0.065906477842411 1108 5 0.15411091385558962 1108 6 0.0846276974557938 1108 7 0.08912197443675751 1108 8 0.11994201702302121 1108 9 0.10956671054356093 1108 10-11 0.18337474943366772 1108 12-13 0.25996180398711033 1108 14-15 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-1.684160516099599 2316 76-77 -1.5903894447908833 2316 78-79 -1.5494220928465054 2316 80-81 -1.5158349232129424 2316 82-83 -1.4093598610761227 2316 84-85 -1.441420411310002 2316 86-87 -1.5636972027778135 2316 88-89 -1.5734894129523056 2316 90-91 -2.029082407271815 2316 92-93 -1.8500717802997357 2316 94-95 -1.707120492475923 2316 96-97 -1.771437481171919 2316 98-99 -2.4855827806288744 2316 100-101 -2.354320254098571 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9765.0 3 1806.0 4 1570.0 5 1762.0 6 2076.0 7 2651.0 8 3373.0 9 3998.0 10 5248.0 11 7337.0 12 11096.0 13 14679.0 14 16684.0 15 20518.0 16 26629.0 17 35084.0 18 28346.0 19 16095.0 20 14908.0 21 15037.0 22 16123.0 23 18423.0 24 20486.0 25 23359.0 26 27054.0 27 31681.0 28 36750.0 29 43395.0 30 51813.0 31 61883.0 32 75257.0 33 94052.0 34 123730.0 35 176392.0 36 240522.0 37 387983.0 38 435037.0 39 79028.0 40 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08725178300759 23.133920208399783 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30.880864004704065 34.44378089340512 17.38223755535547 20-21 18.813486896260986 29.104750235986014 34.10244146502137 17.979321402731628 22-23 18.410060007011005 29.24162182948563 33.889787597877216 18.45853056562615 24-25 18.58167363255571 30.02761395407383 34.10725294675942 17.28345946661105 26-27 18.15826125867385 29.084996551959634 35.45712231310966 17.299619876256855 28-29 17.095792085125634 29.885050601275193 35.3243428638685 17.69481444973067 30-31 17.8827294712914 29.51146434294826 34.67810048154676 17.927705704213587 32-33 17.8437424949936 29.868077198418707 34.54674697721876 17.74143332936893 34-35 18.60853578209865 29.456433344617967 34.50491893463254 17.43011193865085 36-37 18.682393467116665 28.7400483298634 34.75597284269373 17.82158536032621 38-39 17.702859716611623 30.494212453639474 34.28388847112115 17.519039358627744 40-41 17.615797520795137 30.14954010742899 34.74248706462319 17.492175307152678 42-43 17.47835870007515 30.27249417404912 33.52244431619289 18.726702809682845 44-45 18.07686671661647 29.67084890813983 33.55667350888457 18.69561086635913 46-47 18.83798875998409 30.451912686853362 32.865095193212646 17.845003359949903 48-49 18.31689923222647 30.489700394137703 32.655467409489724 18.5379329641461 50-51 17.34648542052389 30.591832419486913 33.20634353830321 18.855338621685988 52-53 17.991282632034025 31.640332183994264 31.846233065348507 18.522152118623207 54-55 17.839238525402195 30.66073179564129 32.24655436517632 19.253475313780193 56-57 18.457993315144847 30.66805537697021 31.761595734868276 19.112355573016664 58-59 18.976423945838548 30.083569431157553 32.06416954889985 18.875837074104055 60-61 18.495602218673152 31.097954460968037 32.34389479809104 18.062548522267768 62-63 17.87420143147097 30.580628420033417 32.85792467765678 18.687245470838835 64-65 17.726598792289263 31.02665077618001 32.223338810933505 19.02341162059722 66-67 18.14789722271572 30.486404461181444 31.111272712544498 20.25442560355834 68-69 17.841823741167794 30.311899765671278 31.875564131089686 19.970712362071243 70-71 18.720666109734122 30.43350984873068 31.77258741194367 19.073236629591527 72-73 18.830069348178665 29.48652984806729 32.25350744936036 19.429893354393677 74-75 17.843599278377084 31.419314458230552 31.326971576893342 19.410114686499025 76-77 18.137713242893284 30.255228862529716 31.793218928910292 19.813838965666708 78-79 18.321501773526393 30.182403756779962 31.727157782780335 19.768936686913303 80-81 18.66781716784857 30.159134004786186 31.138685617577366 20.034363209787877 82-83 18.614291758297757 30.043249030813584 31.815794419980094 19.526664790908573 84-85 18.21719346042151 30.15153540400507 31.48629021812475 20.144980917448667 86-87 18.095979954293504 30.20665923449216 31.669989367052032 20.027371444162302 88-89 18.803543080639596 29.67564707235707 31.143080639595077 20.37772920740826 90-91 18.737726051216107 29.177813993325643 31.685185841449233 20.39927411400902 92-93 19.490098897121175 29.73154391759808 31.48654958191304 19.291807603367708 94-95 19.190062525760894 28.774783677529182 31.571171180293696 20.463982616416228 96-97 19.07584369927638 28.66371677139048 31.736367090509866 20.524072438823275 98-99 19.166848279221995 28.993993783212375 30.87320620684592 20.96595173071971 100-101 19.425895641818734 27.9613076476386 31.626263202601084 20.986533507941576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8201.0 1 5134.5 2 2360.0 3 2573.0 4 2094.5 5 2026.0 6 3413.0 7 6319.0 8 9243.0 9 9617.5 10 7477.0 11 5690.5 12 5563.0 13 5732.0 14 5846.5 15 5967.5 16 6005.5 17 6340.5 18 6718.5 19 7168.5 20 7938.0 21 8943.0 22 10197.5 23 11439.5 24 12936.0 25 15822.0 26 22479.0 27 29252.5 28 30385.0 29 33079.0 30 41653.5 31 50425.5 32 61479.0 33 76678.5 34 93195.0 35 103455.0 36 113275.5 37 122698.0 38 117803.5 39 109032.0 40 100109.5 41 91430.0 42 83851.0 43 77960.0 44 74655.5 45 71723.5 46 68155.5 47 70521.0 48 90323.5 49 84623.5 50 52511.5 51 32437.5 52 21378.5 53 16292.5 54 12832.0 55 10367.5 56 8686.5 57 6906.0 58 5435.0 59 4475.0 60 3680.5 61 2958.5 62 2248.0 63 1549.0 64 1014.5 65 721.5 66 545.0 67 440.5 68 375.0 69 298.0 70 252.0 71 215.5 72 170.0 73 174.0 74 181.0 75 144.0 76 116.0 77 83.0 78 63.5 79 55.5 80 41.5 81 30.5 82 27.0 83 24.5 84 16.0 85 10.0 86 7.0 87 5.0 88 1.5 89 2.5 90 3.0 91 2.0 92 1.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2692405710796941 2 0.15694240983603552 3 0.1847648522339542 4 0.15497146251624885 5 0.22844654515852833 6 0.18508570412322178 7 0.22940910082633112 8 0.23756790601056427 9 0.14044145553084486 10-11 0.19624676627131601 12-13 0.20179292035722732 14-15 0.16370321750274558 16-17 0.2762763946514907 18-19 0.22239619524662507 20-21 0.18914218872324784 22-23 0.2343593871178883 24-25 0.22432130658223068 26-27 0.33315885102021736 28-29 0.304717622835854 30-31 0.2795307495283477 32-33 0.18238138105653778 34-35 0.3503702630802148 36-37 0.3896746195154953 38-39 0.3713860618272424 40-41 0.3357715021185392 42-43 0.3672608232509447 44-45 0.3747779246566426 46-47 0.38027824275837285 48-49 0.39971270005115295 50-51 0.39196641872454957 52-53 0.39231018860590766 54-55 0.38662652656745317 56-57 0.3654273838837013 58-59 0.34156975411744644 60-61 0.31115757861329646 62-63 0.3755571363877211 64-65 0.40810068515629155 66-67 0.41224884172467974 68-69 0.417244964000418 70-71 0.4160532284117098 72-73 0.40739022740148473 74-75 0.40390669260372225 76-77 0.3880474420770668 78-79 0.3628147327853794 80-81 0.3734028451312101 82-83 0.3826617139357893 84-85 0.3837159415719542 86-87 0.3785593933515822 88-89 0.3869473784567208 90-91 0.40789442322747665 92-93 0.40695478555176445 94-95 0.4156177865619895 96-97 0.41584696648289493 98-99 0.413738511210565 100-101 0.3871307223934451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2181692.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.673755376732146 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69245841203869 17.20903478179879 2 11.663210618813778 5.522239901942043 3 4.852533620368244 3.446330816578987 4 2.5814939027583357 2.444546206417056 5 1.6445609386633013 1.9466466682021997 6 1.0617349087983667 1.508115150349773 7 0.7760604294248505 1.286058533463593 8 0.5685341502972058 1.0767470717963454 9 0.4361284191440941 0.929231775689237 >10 2.7927883751683242 12.92956730710218 >50 0.39583087497521363 6.628161659817032 >100 0.45646933543513635 22.505515049405663 >500 0.05473720988011698 8.771833217617067 >1k 0.021699393916760658 8.926824377461742 >5k 7.819601411445282E-4 1.2825067248777517 >10k+ 9.774501764306602E-4 3.5866407574805454 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21676 0.9935407931092014 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18109 0.8300438375352708 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15874 0.7276004128905454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11409 0.5229427435220003 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10431 0.47811515099289903 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8005 0.3669170533695866 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7853 0.3599499837740616 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6720 0.30801781369689213 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5134 0.2353219427856911 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4569 0.209424611723378 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4320 0.19801145166228779 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4309 0.1975072558362959 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3760 0.17234330052088012 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3668 0.16812638997622031 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3661 0.1678055380869527 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3648 0.1672096702925986 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3500 0.16042594463379797 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3461 0.15863834125073567 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2702 0.12384882925729204 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2644 0.1211903421747891 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2625 0.12031945847534849 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2563 0.11747762745612121 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2490 0.11413160061090201 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 2417 0.11078557376568278 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2405 0.11023554195550976 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2384 0.10927298628770697 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2381 0.10913547833516371 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2374 0.10881462644589611 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2321 0.10638531928429862 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2304 0.10560610755322017 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2296 0.10523941967977148 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2267 0.10391017613852001 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1458996045271284E-4 0.0 18-19 0.0 0.0 0.0 2.979338971770534E-4 0.0 20-21 0.0 0.0 0.0 6.646217706257345E-4 0.0 22-23 0.0 0.0 0.0 0.001489669485885267 0.0 24-25 0.0 0.0 0.0 0.0035522887740340983 0.0 26-27 0.0 0.0 0.0 0.006394119793261377 0.0 28-29 0.0 0.0 0.0 0.014415417024951277 0.0 30-31 0.0 0.0 0.0 0.03935019241946159 0.0 32-33 0.0 0.0 0.0 0.08571329041862921 0.0 34-35 0.0 0.0 0.0 0.1530463511806433 0.0 36-37 0.0 0.0 0.0 0.25088325941516953 0.0 38-39 0.0 0.0 0.0 0.40030856784550706 0.0 40-41 0.0 0.0 0.0 0.5715288867539506 0.0 42-43 0.0 0.0 0.0 0.7406865863742453 0.0 44-45 0.0 0.0 0.0 0.9367729267009275 0.0 46-47 0.0 0.0 0.0 1.1758075842052866 0.0 48-49 0.0 0.0 0.0 1.4211217715424542 0.0 50-51 0.0 0.0 0.0 1.7023484524855022 0.0 52-53 0.0 0.0 0.0 1.9803207785516928 0.0 54-55 0.0 0.0 0.0 2.2683082671614505 0.0 56-57 0.0 0.0 0.0 2.618816038194209 0.0 58-59 0.0 0.0 0.0 2.998819265047495 0.0 60-61 0.0 0.0 0.0 3.4197081897903097 0.0 62-63 0.0 0.0 0.0 3.8290235285274 0.0 64-65 0.0 0.0 0.0 4.241684894109709 0.0 66-67 0.0 0.0 0.0 4.70421581048104 0.0 68-69 0.0 0.0 0.0 5.2182434550798185 0.0 70-71 0.0 0.0 0.0 5.80372939901691 0.0 72-73 0.0 0.0 0.0 6.365541057124471 0.0 74-75 0.0 0.0 0.0 6.949033135749684 0.0 76-77 0.0 0.0 0.0 7.539767299875509 0.0 78-79 0.0 0.0 0.0 8.240828677925208 0.0 80-81 0.0 0.0 0.0 8.991782524756015 0.0 82-83 0.0 0.0 0.0 9.719245429694016 0.0 84-85 0.0 0.0 0.0 10.430344888279372 0.0 86-87 0.0 0.0 0.0 11.167250005958678 0.0 88-89 0.0 0.0 0.0 11.957943651074487 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACGC 40 5.424422E-4 47.437363 8 CATTCCG 670 0.0 42.47927 9 TAGGCAT 830 0.0 36.576527 5 GGCATTC 895 0.0 32.86088 7 TTAGGCA 990 0.0 32.102596 4 CCTTTAG 985 0.0 30.885418 1 CTTTAGG 1020 0.0 29.763252 2 GTAAGCG 115 0.0 28.923107 94-95 CATGGGT 2250 0.0 28.671932 4 CATGGGG 4775 0.0 27.716053 4 CGTTCGC 35 0.008424175 27.122957 14-15 GAGACCG 70 0.008449279 27.105816 9 CATATAG 970 0.0 25.918194 3 GTACATG 17710 0.0 25.55219 1 ATGGGTA 1425 0.0 25.298763 5 ACATGGG 16410 0.0 25.177359 3 AACGCCG 95 0.001236712 24.965885 6 GACATAT 1080 0.0 24.647566 1 TACATGG 18190 0.0 24.539036 2 GAGTACT 15100 0.0 24.470724 12-13 >>END_MODULE