##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938862_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2181692 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968030317753378 31.0 31.0 34.0 30.0 34.0 2 32.1635294991227 33.0 31.0 34.0 30.0 34.0 3 32.217534372404536 34.0 31.0 34.0 30.0 34.0 4 35.876515108457106 37.0 35.0 37.0 35.0 37.0 5 35.79751220612259 37.0 35.0 37.0 35.0 37.0 6 35.84861474488608 37.0 35.0 37.0 35.0 37.0 7 35.777056981462096 37.0 35.0 37.0 33.0 37.0 8 35.777909530767865 37.0 35.0 37.0 33.0 37.0 9 37.37115367338745 39.0 37.0 39.0 34.0 39.0 10-11 37.301628965041814 39.0 37.0 39.0 34.0 39.0 12-13 37.24847755778542 39.0 37.0 39.0 33.0 39.0 14-15 38.35991377334656 40.0 38.0 41.0 33.0 41.0 16-17 38.31088622958694 40.0 38.0 41.0 33.0 41.0 18-19 38.54330858801334 40.0 38.0 41.0 34.0 41.0 20-21 38.583814763953846 40.0 38.0 41.0 34.0 41.0 22-23 38.49435988214652 40.0 38.0 41.0 34.0 41.0 24-25 38.40632706174841 40.0 38.0 41.0 34.0 41.0 26-27 38.19968309000537 40.0 37.5 41.0 33.0 41.0 28-29 38.048285917535566 40.0 37.0 41.0 33.0 41.0 30-31 37.7717883642604 40.0 37.0 41.0 33.0 41.0 32-33 37.504370690271585 40.0 36.0 41.0 33.0 41.0 34-35 37.38336735891225 40.0 36.0 41.0 32.0 41.0 36-37 37.20063716601611 40.0 35.5 41.0 31.5 41.0 38-39 37.04150723383502 40.0 35.0 41.0 31.0 41.0 40-41 36.87544300478711 40.0 35.0 41.0 31.0 41.0 42-43 36.60297099682265 40.0 35.0 41.0 30.5 41.0 44-45 36.224434750643084 39.0 35.0 41.0 30.0 41.0 46-47 35.73718494636273 39.0 35.0 41.0 28.0 41.0 48-49 35.29976596146477 39.0 35.0 41.0 25.5 41.0 50-51 35.06639502734575 39.0 35.0 41.0 24.0 41.0 52-53 34.91353958303922 39.0 35.0 40.5 24.0 41.0 54-55 34.68917519063186 39.0 35.0 40.0 23.0 41.0 56-57 34.447955531761586 38.5 34.0 40.0 23.0 41.0 58-59 34.199159643066025 38.0 34.0 40.0 22.0 41.0 60-61 33.84996026020171 38.0 34.0 40.0 20.0 41.0 62-63 33.5462943440229 38.0 33.0 40.0 18.5 41.0 64-65 33.19163474954301 37.0 33.0 40.0 16.5 41.0 66-67 32.79330469195469 37.0 32.5 39.0 13.0 41.0 68-69 32.44770801744701 36.0 32.0 39.0 10.0 40.0 70-71 32.01920367311243 36.0 31.5 39.0 8.0 40.0 72-73 31.51478027145903 35.0 31.0 38.0 4.5 40.0 74-75 30.989582168335403 35.0 31.0 37.0 2.0 39.5 76-77 29.02118676696802 33.0 28.5 35.5 2.0 38.0 78-79 29.94167371012957 34.0 29.5 36.0 2.0 39.0 80-81 29.923582705533136 34.0 30.0 36.0 2.0 38.0 82-83 29.55832239381178 34.0 29.5 35.5 2.0 37.0 84-85 29.10254403463 34.0 29.0 35.0 2.0 37.0 86-87 28.548686524037308 34.0 29.0 35.0 2.0 36.0 88-89 27.90657182590393 34.0 27.5 35.0 2.0 36.0 90-91 27.360148682765487 33.0 26.0 35.0 2.0 35.5 92-93 27.05141811951458 33.0 26.0 35.0 2.0 35.0 94-95 26.684025288629194 33.0 25.5 35.0 2.0 35.0 96-97 26.056072763708165 33.0 24.5 35.0 2.0 35.0 98-99 25.255214989100203 32.5 20.5 34.5 2.0 35.0 100-101 21.6780485054719 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12134392625143775 1101 2 0.05118321194277797 1101 3 -0.04060060247662278 1101 4 0.0013219191787285922 1101 5 -0.099085549969395 1101 6 0.058028586460828535 1101 7 0.03726634633312642 1101 8 0.15702464202337296 1101 9 0.1631463728284075 1101 10-11 0.1481317719949189 1101 12-13 0.08983437159483998 1101 14-15 0.09836288967657225 1101 16-17 0.21052621925812076 1101 18-19 0.28546225424273786 1101 20-21 0.21393041230598442 1101 22-23 0.03862943755762416 1101 24-25 0.13318369546310294 1101 26-27 -0.016185863360355768 1101 28-29 0.06282326842552521 1101 30-31 0.14051313692799994 1101 32-33 0.21324444769992823 1101 34-35 0.26913171938109315 1101 36-37 0.2978289443183044 1101 38-39 0.29802257026431533 1101 40-41 0.4220688403262258 1101 42-43 0.5071451446685913 1101 44-45 0.45019277553792136 1101 46-47 0.37455544146356345 1101 48-49 0.391546287886527 1101 50-51 0.30536974768790515 1101 52-53 0.18492962272785718 1101 54-55 0.16436213913095798 1101 56-57 0.24554364009769358 1101 58-59 0.1556861069584201 1101 60-61 0.18115894809049138 1101 62-63 -0.0126634814601303 1101 64-65 0.04599225469093682 1101 66-67 0.11878611812334583 1101 68-69 0.1713864204591644 1101 70-71 0.08778086904660398 1101 72-73 0.0021650594420208336 1101 74-75 0.049037907727452534 1101 76-77 0.09801591890016681 1101 78-79 -0.02246889319134837 1101 80-81 0.05100097476529086 1101 82-83 0.08842588595256018 1101 84-85 0.08373822174347012 1101 86-87 0.19441969352039834 1101 88-89 0.22121650896744072 1101 90-91 0.1992583285275522 1101 92-93 0.3083887316676659 1101 94-95 0.2254256057141184 1101 96-97 0.2569806728228947 1101 98-99 0.2555380364143076 1101 100-101 1.012325626609229 1102 1 0.27412786111725396 1102 2 0.17010571552891207 1102 3 0.22460575307989217 1102 4 0.1228335533720113 1102 5 0.0025311812115376142 1102 6 -0.012964140366300114 1102 7 0.1149911731537614 1102 8 0.16133700641726278 1102 9 0.20600871595782166 1102 10-11 0.24264652738131787 1102 12-13 0.2865053542300515 1102 14-15 0.2903536594507301 1102 16-17 0.3558858322445886 1102 18-19 0.30541459838130436 1102 20-21 0.3501136364336048 1102 22-23 0.3449726498636281 1102 24-25 0.31211820987608974 1102 26-27 0.317263253262702 1102 28-29 0.3470527734316846 1102 30-31 0.351040045505151 1102 32-33 0.46807986423143433 1102 34-35 0.5072815024113169 1102 36-37 0.444482897349026 1102 38-39 0.5148074079694922 1102 40-41 0.517712820676401 1102 42-43 0.32340427217618384 1102 44-45 0.2940042713318789 1102 46-47 0.2995215624620613 1102 48-49 0.22700606918675703 1102 50-51 0.3483940522673379 1102 52-53 0.008532879797392923 1102 54-55 0.14695387373583202 1102 56-57 0.06491337353482862 1102 58-59 0.2169201495882973 1102 60-61 0.1552773281696389 1102 62-63 0.1701101275185266 1102 64-65 0.15378537112657398 1102 66-67 0.14074619856794612 1102 68-69 0.22199971147978204 1102 70-71 0.3444643130273164 1102 72-73 0.08832764981605834 1102 74-75 0.07665785380938317 1102 76-77 0.011501248211271076 1102 78-79 -0.012955516391972566 1102 80-81 0.14158315296883828 1102 82-83 0.07256920674345224 1102 84-85 0.12094205079147358 1102 86-87 0.23662022477197198 1102 88-89 0.07149474927768651 1102 90-91 0.060686905104905264 1102 92-93 0.10803228132202847 1102 94-95 0.3670319479352848 1102 96-97 0.33544164709647717 1102 98-99 0.2528707408236279 1102 100-101 1.0751777069240873 1103 1 0.1361448048341778 1103 2 0.13769840710337178 1103 3 0.08219033803900544 1103 4 0.0379145304418671 1103 5 -0.05295815547133742 1103 6 0.02160701077308147 1103 7 0.04479011127623522 1103 8 -0.014561847966547248 1103 9 0.08185946515160936 1103 10-11 0.12466209030113617 1103 12-13 0.1390449073862854 1103 14-15 0.21470668918686187 1103 16-17 0.1262191833946602 1103 18-19 -0.033379564855643196 1103 20-21 0.07609734302757687 1103 22-23 0.15831293172440297 1103 24-25 0.18287493124013565 1103 26-27 0.05990516357324083 1103 28-29 0.13816533957632515 1103 30-31 0.16552393917486796 1103 32-33 0.3067648999539969 1103 34-35 0.24721125992552118 1103 36-37 0.3648253795768355 1103 38-39 0.24490522185585206 1103 40-41 0.2885305643648195 1103 42-43 0.34040166202419897 1103 44-45 0.27366245723170834 1103 46-47 0.45510599597890433 1103 48-49 0.4974659191698336 1103 50-51 0.607171022353036 1103 52-53 0.5788704624225147 1103 54-55 0.4039226677209058 1103 56-57 0.4151020082382715 1103 58-59 0.5495036210013424 1103 60-61 0.5979339372882393 1103 62-63 0.5099726268503986 1103 64-65 0.49376969239797575 1103 66-67 0.4146110497091158 1103 68-69 0.40412172940342117 1103 70-71 0.3761756551621147 1103 72-73 0.5384390044225746 1103 74-75 0.5341146036802122 1103 76-77 0.5331421251510662 1103 78-79 0.21043385057935993 1103 80-81 0.4874819628108007 1103 82-83 0.5762040438093905 1103 84-85 0.7374505939168827 1103 86-87 0.588912347992526 1103 88-89 0.43468871118549757 1103 90-91 0.36285475658458566 1103 92-93 0.3328630223201223 1103 94-95 0.5445536843544758 1103 96-97 0.6621549490723524 1103 98-99 0.9570692549077542 1103 100-101 1.654840569717397 1104 1 0.05394866617687555 1104 2 0.05928901702000644 1104 3 0.10473092601618816 1104 4 0.016111107888114873 1104 5 -0.071049423571381 1104 6 0.012456067376859892 1104 7 -0.013483254234955666 1104 8 -0.08307654739611792 1104 9 -0.012197138621971249 1104 10-11 0.027515315399860185 1104 12-13 -0.07761481178879848 1104 14-15 -0.09869063420058666 1104 16-17 0.07714502806337009 1104 18-19 0.06685738117332107 1104 20-21 0.016796114418539787 1104 22-23 -0.03525881026769895 1104 24-25 0.02028936739634446 1104 26-27 0.07908353127837842 1104 28-29 -0.03525502023060767 1104 30-31 -0.11603266459871975 1104 32-33 -0.09275901404337361 1104 34-35 -0.1318266953180114 1104 36-37 0.03239777975235114 1104 38-39 -0.012905221320984595 1104 40-41 0.03452113040255966 1104 42-43 -0.03331597729128788 1104 44-45 0.05842551120274919 1104 46-47 -0.055862630611706265 1104 48-49 0.04305170240809275 1104 50-51 -0.02662230805503185 1104 52-53 -0.00711790967051229 1104 54-55 8.522420956253995E-4 1104 56-57 -0.08652918965554335 1104 58-59 0.061911475323405796 1104 60-61 0.14942252877573736 1104 62-63 0.14063848907066756 1104 64-65 -0.045857337000882126 1104 66-67 -0.013674382497995907 1104 68-69 0.05482708262852043 1104 70-71 -0.07988511710642143 1104 72-73 0.0686891141198096 1104 74-75 0.18941736805054887 1104 76-77 0.23966801369867596 1104 78-79 -0.02441257329076052 1104 80-81 0.2936239110337091 1104 82-83 0.13247324082562884 1104 84-85 0.30813729861192485 1104 86-87 0.45720107111670316 1104 88-89 0.4824432087721604 1104 90-91 0.6007024968215298 1104 92-93 0.7074549486035799 1104 94-95 0.5163516220464466 1104 96-97 0.548285927571694 1104 98-99 0.9884119490718639 1104 100-101 1.6768820352724667 1105 1 0.04291322372605677 1105 2 0.07266522145189214 1105 3 0.008599445276558981 1105 4 0.05472545775845106 1105 5 -0.05868610155320653 1105 6 -0.025748828362033294 1105 7 0.008302573001550684 1105 8 0.05170639131226551 1105 9 -0.011417446872201253 1105 10-11 0.08077513534124847 1105 12-13 0.11193124652995579 1105 14-15 -0.007628836566226482 1105 16-17 0.027778395834140213 1105 18-19 0.061208094871695096 1105 20-21 0.0383548653936856 1105 22-23 -0.029967743620709086 1105 24-25 -0.059057234893387545 1105 26-27 -0.022203023361193175 1105 28-29 0.021567809683574524 1105 30-31 -0.04195288218711113 1105 32-33 -0.07065987638497262 1105 34-35 -0.13703047359712173 1105 36-37 -0.1706364398179545 1105 38-39 -0.03096590149555567 1105 40-41 -0.24639455409427313 1105 42-43 -0.23166529943596714 1105 44-45 -0.19850906468963814 1105 46-47 -0.42989096770504176 1105 48-49 -0.3656980076848839 1105 50-51 -0.37793606124401435 1105 52-53 -0.3935116771967131 1105 54-55 -0.3085995700867912 1105 56-57 -0.2258410031030138 1105 58-59 -0.20924203834965738 1105 60-61 -0.2919083025721747 1105 62-63 -0.3886505534982234 1105 64-65 -0.45580778925582877 1105 66-67 -0.32452428445671444 1105 68-69 -0.3070837735986842 1105 70-71 -0.2896997874557883 1105 72-73 -0.24354580544416748 1105 74-75 -0.23042935020199806 1105 76-77 -0.12532513395360567 1105 78-79 -0.3648321478126775 1105 80-81 -0.35980352787497694 1105 82-83 -0.19599035739486936 1105 84-85 -0.14178827434976427 1105 86-87 0.0225718358387077 1105 88-89 -0.08773790404910997 1105 90-91 -0.14819016378695693 1105 92-93 0.08795590793226182 1105 94-95 0.4274252268030274 1105 96-97 0.3231192573910029 1105 98-99 0.38960573346537686 1105 100-101 1.0400244165160082 1106 1 0.08602868798575614 1106 2 0.061821363361772796 1106 3 0.01404038142332098 1106 4 0.04600017618765406 1106 5 0.04587216931556526 1106 6 0.11802865529389805 1106 7 0.1288268093043783 1106 8 0.07013837490975305 1106 9 0.09382630592266139 1106 10-11 0.09866531435135784 1106 12-13 0.10264270074082305 1106 14-15 0.048658957931444036 1106 16-17 0.1662263290468715 1106 18-19 -0.04836416767647478 1106 20-21 0.050642445703793726 1106 22-23 0.11935041237159538 1106 24-25 0.041023739447432206 1106 26-27 0.039554728879402035 1106 28-29 0.15360462705271516 1106 30-31 0.057889788061338265 1106 32-33 -0.0532845241404587 1106 34-35 -0.04409167319457907 1106 36-37 0.030282318515538975 1106 38-39 0.12610960452254005 1106 40-41 0.19520643247649616 1106 42-43 0.2430270512070436 1106 44-45 0.212519918483558 1106 46-47 0.17776770889381766 1106 48-49 0.015889792453599227 1106 50-51 0.10486782552434448 1106 52-53 0.11010355529897708 1106 54-55 0.11435340593482834 1106 56-57 0.05882468037192723 1106 58-59 0.053190649941228685 1106 60-61 0.18800977477570058 1106 62-63 0.1403197439200028 1106 64-65 0.10772498103128214 1106 66-67 0.00917461198687164 1106 68-69 -0.018562101006430964 1106 70-71 -0.012432771603450021 1106 72-73 0.0744879691536795 1106 74-75 0.1999290378976788 1106 76-77 0.1834728242340411 1106 78-79 0.026842905141517548 1106 80-81 -0.06005172383533264 1106 82-83 0.13243968870450828 1106 84-85 0.10669749321422728 1106 86-87 0.2916089810292597 1106 88-89 0.39951998021930635 1106 90-91 0.3577077603105323 1106 92-93 0.32401900169981346 1106 94-95 0.5923719210797245 1106 96-97 0.8059601364755871 1106 98-99 0.8561360667559264 1106 100-101 1.2538357123752384 1107 1 0.11732778905880181 1107 2 0.03869017720705159 1107 3 -0.0024157845306831405 1107 4 -0.030605271161860514 1107 5 -0.03291447111405432 1107 6 -0.08832315340236363 1107 7 -0.013091873362419904 1107 8 -0.01894528170912224 1107 9 -0.04781738044911066 1107 10-11 -0.03760554609630162 1107 12-13 0.021087320080944494 1107 14-15 0.054856267975026185 1107 16-17 0.11960527030709756 1107 18-19 -0.08744635037981396 1107 20-21 -0.11297913839600682 1107 22-23 0.04321732466393513 1107 24-25 -0.009648769847316885 1107 26-27 -0.06034585248941937 1107 28-29 -0.0033181119103815604 1107 30-31 -0.03494892075635647 1107 32-33 6.395841507398359E-4 1107 34-35 0.1291746689953044 1107 36-37 0.05890232572762244 1107 38-39 0.06560163669670516 1107 40-41 -0.18238437788226491 1107 42-43 -0.0809925829630771 1107 44-45 -0.11628105728890858 1107 46-47 -0.13118043229679444 1107 48-49 0.09615327605497725 1107 50-51 -0.035321732127357564 1107 52-53 -0.1385108631484684 1107 54-55 0.03174831474792228 1107 56-57 -0.13896277050933747 1107 58-59 -0.06434936888450693 1107 60-61 0.01738688999591176 1107 62-63 -0.013392483965915858 1107 64-65 0.017797356341063164 1107 66-67 0.04866171764884797 1107 68-69 0.1590458310012366 1107 70-71 -0.03613908767474783 1107 72-73 -0.09312146503639696 1107 74-75 0.025819434308271383 1107 76-77 -0.19874375897131458 1107 78-79 -0.06068778965418886 1107 80-81 -0.020389565465738002 1107 82-83 -0.18354962964792065 1107 84-85 -0.10850649063811701 1107 86-87 -0.015211472234092582 1107 88-89 0.0378377660993543 1107 90-91 0.3567062044210694 1107 92-93 0.36569790051934703 1107 94-95 0.325450966967761 1107 96-97 0.46558115039217896 1107 98-99 0.6961304214575357 1107 100-101 1.3578257026716223 1108 1 0.1263185979628254 1108 2 0.10393477671743057 1108 3 0.07162302174099011 1108 4 0.0776915126698583 1108 5 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-1.0386656359099362 2316 50-51 -1.1653935434170677 2316 52-53 -1.1613679142520397 2316 54-55 -1.098512208946211 2316 56-57 -1.1053072056725526 2316 58-59 -0.8564992857592912 2316 60-61 -0.8718218130425797 2316 62-63 -0.8339148123752551 2316 64-65 -0.9825346328023699 2316 66-67 -0.9539615523992211 2316 68-69 -0.9408362303628479 2316 70-71 -0.8917888318057834 2316 72-73 -0.8815009274804204 2316 74-75 -1.1192660853187029 2316 76-77 -1.0938894477707102 2316 78-79 -1.0159318065269645 2316 80-81 -1.0084758928770832 2316 82-83 -1.046922731098796 2316 84-85 -1.2700731582576132 2316 86-87 -1.3701751819300085 2316 88-89 -1.741960001959601 2316 90-91 -2.273778233290077 2316 92-93 -1.911216776065178 2316 94-95 -1.7804554225042928 2316 96-97 -1.8232415524070475 2316 98-99 -1.8723544482012144 2316 100-101 -2.306852822791935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 21.0 5 87.0 6 255.0 7 524.0 8 1029.0 9 1928.0 10 3504.0 11 5612.0 12 8043.0 13 11354.0 14 13777.0 15 18793.0 16 24956.0 17 35194.0 18 30553.0 19 17077.0 20 14055.0 21 13797.0 22 14741.0 23 16543.0 24 18835.0 25 21789.0 26 25750.0 27 31423.0 28 37756.0 29 45100.0 30 55021.0 31 65719.0 32 80177.0 33 100650.0 34 131030.0 35 183121.0 36 256199.0 37 428724.0 38 425208.0 39 43326.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.24361783743804 24.14522434616427 16.284908373415657 19.326249442982032 2 13.940922916708685 24.605352176200856 40.72160506615966 20.732119840930803 3 17.90834819947087 30.636038450890407 32.69178234141208 18.763831008226642 4 10.46568443208299 20.576873362509467 42.31138034149642 26.64606186391113 5 9.541055326539418 42.0479132021958 36.535066061642254 11.87596540962253 6 23.69484244233856 42.9915649606209 19.371621194216758 13.94197140282378 7 23.061321213076823 37.143923156889244 24.24728146777822 15.547474162255718 8 23.851396072406185 35.53155074135121 21.652735583207896 18.964317603034708 9 25.152175467481204 18.390130229198256 24.66379305603174 31.7939012472888 10-11 21.983854732932055 29.39851271398529 29.483332202712393 19.13430035037026 12-13 24.250810838560163 28.624824219000665 29.14850033826956 17.975864604169608 14-15 21.6297030011569 29.125674018147386 29.749410090883586 19.495212889812127 16-17 17.298313276427766 32.281426789009664 30.7962730550516 19.623986879510973 18-19 17.166974073333908 31.0618318259406 34.27069448849792 17.500499612227575 20-21 18.61160970476126 29.272532511463577 34.048412883211746 18.067444900563416 22-23 18.20625917865583 29.43332514397083 33.7744512057614 18.58596447161194 24-25 18.376663855377508 30.167646034248836 34.10807267793627 17.34761743243739 26-27 18.047887791993492 29.202431631840398 35.40082391295629 17.348856663209823 28-29 16.862806482308226 30.06022389961553 35.331660014337494 17.74530960373875 30-31 17.72074151621769 29.659846577793747 34.609376575611954 18.010035330376606 32-33 17.678778672699906 30.022592556602856 34.506314365180785 17.79231440551645 34-35 18.44360680641256 29.53111828344406 34.52089381264809 17.50438109749529 36-37 18.508876808438263 28.829226764565384 34.793783712663355 17.868112714332998 38-39 17.506113657529284 30.653829571238294 34.22843682217709 17.61161994905534 40-41 17.475971599110878 30.290843697469743 34.635804758327026 17.59737994509235 42-43 17.397730919092368 30.3926197570479 33.47073114829246 18.73891817556727 44-45 17.96683626696003 29.803034470113676 33.46456729006234 18.765561972863953 46-47 18.711434572041206 30.621717555537426 32.7618782205656 17.90496965185577 48-49 18.206384540582736 30.5912171027527 32.53503466740128 18.66736368926329 50-51 17.22756211927451 30.776278939721042 32.96398663421476 19.03217230678969 52-53 17.901243621922802 31.76981443760164 31.62352889408771 18.70541304638785 54-55 17.75211624738964 30.79376007245752 31.95996501797687 19.494158662175963 56-57 18.376176111848828 30.79573847241938 31.469170938263037 19.35891447746875 58-59 18.771457068445354 30.337487314833233 31.759967767988183 19.131087848733223 60-61 18.272680247805713 31.36716448286223 32.01798564696052 18.342169622371546 62-63 17.673300297342255 30.869965590437744 32.498426657487116 18.958307454732886 64-65 17.472532937671044 31.312806289343637 31.882052117689362 19.33260865529596 66-67 17.900437206462477 30.77483362960206 30.72798680588088 20.59674235805458 68-69 17.60167833655103 30.569023744960315 31.44960498403985 20.3796929344488 70-71 18.444133472613675 30.61620550297911 31.42172745819642 19.517933566210797 72-73 18.567492569986964 29.69892175430812 31.805383161326162 19.92820251437875 74-75 17.486265785666273 31.670751045120372 30.772294736205225 20.070688433008133 76-77 17.76381760325725 30.33891258770684 31.465082251064384 20.432187557971528 78-79 17.872432130723592 30.320922122950183 31.32747156808226 20.479174178243966 80-81 18.16748798278009 30.301751738698172 30.78406773554645 20.746692542975286 82-83 18.069116493393004 30.260679403272135 31.2789386269062 20.391265476428664 84-85 17.514204571497718 30.305538086952694 30.98377314488021 21.196484196669374 86-87 17.349955596524165 30.45316781118509 31.228731698621647 20.968144893669102 88-89 17.985797490503604 29.872993428648442 30.641744043878926 21.499465036969028 90-91 17.901292512593127 29.288393339066793 31.202567263433934 21.607746884906145 92-93 18.450931243994017 30.02706402432806 30.959442465972874 20.562562265705054 94-95 18.102527997829203 28.955322188746596 31.044210283143276 21.897939530280926 96-97 17.934866060194647 28.9621939699013 31.177770983857002 21.92516898604705 98-99 17.911327538442638 29.24718521221144 30.297608461689368 22.543878787656553 100-101 17.77606603409054 28.301463884627353 31.3767354323151 22.545734648967002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6646.0 1 4092.5 2 1784.5 3 2056.0 4 1700.5 5 1608.0 6 3012.0 7 5780.5 8 8345.0 9 8829.0 10 6912.5 11 5092.0 12 4968.5 13 5266.5 14 5411.0 15 5516.0 16 5687.5 17 6017.5 18 6397.0 19 6968.0 20 7821.5 21 8844.5 22 10112.0 23 11329.0 24 12704.5 25 15898.5 26 22900.0 27 29302.5 28 30369.0 29 33851.0 30 42481.5 31 50630.0 32 61975.0 33 77295.0 34 92893.5 35 103089.0 36 113815.0 37 123050.5 38 118528.0 39 110335.5 40 100608.0 41 91114.0 42 83049.5 43 77400.5 44 74452.0 45 71015.5 46 67009.0 47 71584.5 48 93390.5 49 85463.5 50 51662.0 51 33678.5 52 24525.5 53 18585.5 54 13728.5 55 10561.0 56 8548.5 57 6707.0 58 5230.0 59 4202.5 60 3389.5 61 2738.5 62 2016.5 63 1334.0 64 815.0 65 551.0 66 419.0 67 344.0 68 273.0 69 219.0 70 166.5 71 131.5 72 120.0 73 132.0 74 133.5 75 106.0 76 76.0 77 55.5 78 38.0 79 34.5 80 27.0 81 17.0 82 19.0 83 15.0 84 5.0 85 2.0 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22560471413930108 2 0.0 3 0.0 4 0.0 5 0.02855581814481604 6 0.003575206766124641 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0030251749559516193 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.500318101730216E-4 26-27 0.0013521615333420116 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007379593453154707 36-37 0.017876033830623205 38-39 0.014002893167321511 40-41 0.009671392662208964 42-43 0.008800508962768346 44-45 0.004216910544659833 46-47 4.5835984181085137E-4 48-49 0.001443833501704182 50-51 9.167196836217027E-5 52-53 0.0 54-55 0.0 56-57 0.0015125874779758097 58-59 4.5835984181085137E-4 60-61 0.0029105849954989063 62-63 0.0014209155096136393 64-65 0.001535505470066352 66-67 0.005225302196643706 68-69 3.666878734486811E-4 70-71 4.583598418108514E-5 72-73 0.0 74-75 2.520979129959682E-4 76-77 0.055163606961935965 78-79 0.14257282879526534 80-81 0.0014209155096136393 82-83 0.0012604895649798414 84-85 0.0 86-87 0.10292470247862669 88-89 0.3533954380361664 90-91 0.38882665380814524 92-93 6.187857864446494E-4 94-95 2.2917992090542568E-4 96-97 1.375079525432554E-4 98-99 0.0 100-101 0.0014209155096136393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2181692.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.83856950721639 #Duplication Level Percentage of deduplicated Percentage of total 1 70.06474932861137 15.301138982185725 2 12.801319133273001 5.59124995352083 3 5.2807771893223325 3.459738591034157 4 2.85460461483068 2.493619251864019 5 1.7468045255343374 1.9073856023200886 6 1.1601226638185773 1.520125165841942 7 0.8288684863097341 1.2670911435431456 8 0.6351293897024632 1.1096253858474534 9 0.4864685109365475 0.9561398750243866 >10 3.115856984197473 13.250602226094225 >50 0.43154060146482454 6.708948359500084 >100 0.5055370046195745 23.077154093592416 >500 0.061902484032050877 9.170910221783902 >1k 0.024424109345979262 9.381506364196525 >5k 8.422106671027331E-4 1.2701522362148177 >10k+ 0.0010527633338784162 3.534612547436304 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19919 0.9130069689030349 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18746 0.8592413594586221 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16477 0.7552395113517398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11671 0.5349517713774447 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10057 0.46097249290917325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8207 0.37617592217416573 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7706 0.3532120940994421 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6581 0.30164661189572134 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5129 0.2350927628647857 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4950 0.22688812169637143 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4780 0.21909600438558696 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4750 0.2177209248601544 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3976 0.1822438731039945 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3887 0.17816447051187792 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3773 0.17293916831523423 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3731 0.17101405697962865 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3719 0.17046402516945564 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3500 0.16042594463379797 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 3006 0.13778296844834192 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2951 0.13526198931838224 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2718 0.12458220500418941 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2687 0.12316128949457576 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2631 0.120594474380435 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2485 0.11390242068999656 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2459 0.11271068510128836 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2436 0.1116564574651234 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2392 0.10963967416115566 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 2384 0.10927298628770697 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2366 0.10844793857244744 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2282 0.1045977159012363 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2244 0.10285594850235505 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2236 0.10248926062890638 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2195 0.10060998527748187 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1458996045271284E-4 0.0 18-19 0.0 0.0 0.0 2.979338971770534E-4 0.0 20-21 0.0 0.0 0.0 5.729498022635642E-4 0.0 22-23 0.0 0.0 0.0 0.0015125874779758097 0.0 24-25 0.0 0.0 0.0 0.0035522887740340983 0.0 26-27 0.0 0.0 0.0 0.006417037785351919 0.0 28-29 0.0 0.0 0.0 0.01453000698540399 0.0 30-31 0.0 0.0 0.0 0.039991896197996785 0.0 32-33 2.291799209054257E-5 0.0 0.0 0.0871571239203334 0.0 34-35 4.583598418108514E-5 0.0 0.0 0.15565900227896512 0.0 36-37 4.583598418108514E-5 0.0 0.0 0.25450430216547526 0.0 38-39 6.87539762716277E-5 0.0 0.0 0.4063130817732292 0.0 40-41 9.167196836217027E-5 0.0 0.0 0.5802606417404473 0.0 42-43 9.167196836217027E-5 0.0 0.0 0.7514580426568003 0.0 44-45 9.167196836217027E-5 2.291799209054257E-5 0.0 0.9492861503823637 0.0 46-47 9.167196836217027E-5 4.583598418108514E-5 0.0 1.1902459192223285 0.0 48-49 9.167196836217027E-5 4.583598418108514E-5 0.0 1.438814461436353 0.0 50-51 9.167196836217027E-5 4.583598418108514E-5 0.0 1.7233642512325296 0.0 52-53 9.167196836217027E-5 4.583598418108514E-5 0.0 2.003742966468227 0.0 54-55 9.167196836217027E-5 4.583598418108514E-5 0.0 2.2945722860972126 0.0 56-57 9.167196836217027E-5 4.583598418108514E-5 0.0 2.64801356011756 0.0 58-59 9.167196836217027E-5 4.583598418108514E-5 0.0 3.031454485784428 0.0 60-61 9.167196836217027E-5 4.583598418108514E-5 0.0 3.458554186383779 0.0 62-63 9.167196836217027E-5 4.583598418108514E-5 0.0 3.871971845705077 0.0 64-65 9.167196836217027E-5 4.583598418108514E-5 0.0 4.287658386243338 0.0 66-67 9.167196836217027E-5 4.583598418108514E-5 0.0 4.754772901032776 0.0 68-69 9.167196836217027E-5 4.583598418108514E-5 0.0 5.272811194247401 0.0 70-71 9.167196836217027E-5 4.583598418108514E-5 0.0 5.861620247037621 0.0 72-73 9.167196836217027E-5 4.583598418108514E-5 0.0 6.428107175531652 0.0 74-75 9.167196836217027E-5 4.583598418108514E-5 0.0 7.017145408242777 0.0 76-77 9.167196836217027E-5 4.583598418108514E-5 0.0 7.6135632344070565 0.0 78-79 9.167196836217027E-5 4.583598418108514E-5 0.0 8.318818605009323 0.0 80-81 9.167196836217027E-5 4.583598418108514E-5 0.0 9.07428729628197 0.0 82-83 9.167196836217027E-5 4.583598418108514E-5 0.0 9.806081701725084 0.0 84-85 9.167196836217027E-5 4.583598418108514E-5 0.0 10.52098554699747 0.0 86-87 9.167196836217027E-5 4.583598418108514E-5 0.0 11.261809641324255 0.0 88-89 9.167196836217027E-5 4.583598418108514E-5 0.0 12.053282498171143 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAACCG 60 8.336581E-5 39.5483 5 CATTCCG 660 0.0 36.657776 9 TACGGGA 70 2.0646442E-4 33.89855 4 GTACGGG 60 0.0039688875 31.638641 3 CTTAACG 30 0.00397556 31.62849 20-21 GAACCGT 110 2.761266E-6 30.190138 6 TGCCGTT 40 4.528156E-4 29.651031 94-95 CATATAG 1125 0.0 27.842005 3 GGCATTC 895 0.0 27.5626 7 GACATAT 1215 0.0 27.409908 1 TTAGGCA 920 0.0 27.339912 4 TAGGCAT 920 0.0 26.308218 5 GTAAGCG 175 0.0 25.754038 94-95 CCTTTAG 1035 0.0 25.281809 1 TAGGACA 1130 0.0 25.198917 4 CATGGGT 2020 0.0 25.138626 4 GACAATG 840 0.0 23.719736 7 AGTGGCG 100 0.0016664414 23.719736 9 CATGGGG 4610 0.0 23.574564 4 GTACATG 17640 0.0 23.54515 1 >>END_MODULE