FastQCFastQC Report
Thu 26 May 2016
SRR938861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6551321
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT3689755.63207023438479No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA2377093.628413261997084No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2205833.3670003347416495No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA2128713.249283617761975No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA1744812.663294929373786No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1440482.198762661759361No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1241531.895083449582153No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1218821.8604186850255087No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1146901.7506392985475752No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1125671.7182336203645034No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA998351.5238911358487852No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT982851.500231785314748No Hit
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG623480.9516859271588127No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA373620.5702971965501309No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT360300.5499654191879775No Hit
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG351270.5361819394897609No Hit
CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG346590.5290383420381936No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT332740.5078975675287473No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG311110.4748813254609261No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG304250.4644101548374748No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA294200.4490697372331473No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA286380.4371332132862975No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA254090.3878454436899062No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC226810.3462049867500005No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA219610.3352148368245122No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG209440.31969125005475996No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA208330.3179969352745805No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT205690.3139672136352348No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT203670.31088386601725054No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT199870.3050835091121318No Hit
GCTTATACTCTGCGTTGATACCACTGCTTTTACTCTGCGTTGATACCACT195660.29865732422514485No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT191720.2926432699603637No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT188190.2872550436774507No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG181250.2766617602770495No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA177270.2705866496237934No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG170120.25967282018389876No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC162770.24845370880162945No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG162640.24825527553908594No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG162480.2480110499851862No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC158280.241600129195318No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCA158050.24124905496158713No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA148750.22705344464116475No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG143990.21978773441264748No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTAT136820.20884337677851533No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAG131350.20049391565456798No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC125210.19112176002366546No Hit
GTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCAGA124650.19026697058501638No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATA121720.18579459012922736No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG117060.17868152087189743No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA115200.17584239880781297No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG112360.17150739522609257No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA112150.17118684918659915No Hit
AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAT111410.17005730599981286No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA111060.16952306260065717No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG110950.1693551575323511No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG110230.16825614253980228No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGT107330.1638295543753695No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGT106520.16259316250875205No Hit
ATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG104280.1591740047541557No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA103900.15859396906364379No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT102340.15621276991312133No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG100770.15381630666548013No Hit
GTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGC100670.1536636656942928No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA96460.1472374808073059No Hit
GATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTT95510.1457873915810262No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG92920.14183399042727413No Hit
GCTTCTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT91880.1402465243269258No Hit
GTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGCTT88550.13516357998638748No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA88520.13511778769503127No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG87990.13430879054773837No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCA85120.1299279946746618No Hit
GCTTACACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT82150.12539455783039788No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCA79160.12083059279189648No Hit
TGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGT78980.12055583904375927No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA77810.11876993968086742No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGT76540.11683139934678823No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAG76080.11612925087932646No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTAT75110.11464863345880931No Hit
TACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGA74800.11417544644812855No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAG74570.11382437221439767No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT73950.11287799819303618No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG73940.11286273409591745No Hit
ATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGT72110.11006940432318918No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA68530.10460485755468248No Hit
GAGTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGC65700.10028511807008082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC8100.031.70377594-95
ACCACTC31400.029.83587694-95
CTTAGGG39250.029.07861994-95
GCCGGAT4550.027.17466494-95
TACGTTA700.00849704727.0753044
GCCGTAT14550.026.80112894-95
GCCGTAG1250.026.63117294-95
GCTTCCC23950.026.5248551
CGTATCG458.8915287E-426.41981194-95
TACCTGG7000.026.3984242
TTATGTA6150.026.1948073
CGTATCC751.0507392E-625.3630294-95
ACACTCT75700.025.1619835
GCTTACA84700.024.9634841
CTTCCCA25350.024.485262
TACACTC79150.024.304494
GCGCCGT5200.024.23528392-93
CTTACAC81750.023.995182
CGTATCT1102.1445885E-923.7778394-95
GTACTAA1801.9907748E-723.7041131
Produced by FastQC (version 0.11.5)