##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938861_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6551321 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86825160910296 33.0 31.0 34.0 30.0 34.0 2 31.674901748822872 33.0 31.0 34.0 30.0 34.0 3 31.868358610423762 33.0 31.0 34.0 30.0 34.0 4 35.10799440296087 37.0 35.0 37.0 33.0 37.0 5 35.30170220631839 37.0 35.0 37.0 33.0 37.0 6 35.44601294914415 37.0 35.0 37.0 33.0 37.0 7 35.51654712080205 37.0 35.0 37.0 33.0 37.0 8 35.53527799965839 37.0 35.0 37.0 33.0 37.0 9 37.00754092189957 39.0 37.0 39.0 33.0 39.0 10-11 36.82665503644227 39.0 37.0 39.0 32.5 39.0 12-13 36.66821569268244 39.0 37.0 39.0 32.5 39.0 14-15 37.44216265696643 39.0 37.0 41.0 32.0 41.0 16-17 37.29497417391088 39.0 36.5 41.0 32.0 41.0 18-19 37.81138246774964 40.0 37.5 41.0 32.0 41.0 20-21 37.85222942060082 40.0 37.5 41.0 32.5 41.0 22-23 37.73812167042342 40.0 37.5 41.0 32.0 41.0 24-25 37.919686350279584 40.0 38.0 41.0 32.5 41.0 26-27 37.36666559309184 39.5 37.0 41.0 31.0 41.0 28-29 37.00391989646057 39.0 36.5 41.0 30.5 41.0 30-31 37.270254579190976 39.0 36.5 41.0 31.0 41.0 32-33 37.583238403369336 40.0 37.5 41.0 31.5 41.0 34-35 37.45965187784265 40.0 37.0 41.0 31.0 41.0 36-37 36.81640794276452 39.0 36.0 41.0 30.0 41.0 38-39 36.15029197928173 38.5 35.0 40.5 29.5 41.0 40-41 35.73118345139858 38.0 35.0 40.0 29.5 41.0 42-43 36.200027826449045 38.5 35.5 40.0 30.0 41.0 44-45 36.94202634857916 39.5 37.0 41.0 30.0 41.0 46-47 36.72147220385018 40.0 36.5 41.0 30.0 41.0 48-49 36.7335519813485 40.0 36.5 41.0 30.0 41.0 50-51 35.65660276759451 38.5 35.0 40.0 28.5 40.5 52-53 35.33676116618312 38.0 34.5 39.5 27.5 40.5 54-55 35.500474484458934 38.0 34.5 40.0 28.0 41.0 56-57 36.230844741083516 39.0 36.0 40.5 28.0 41.0 58-59 36.47576801686256 40.0 36.0 41.0 29.0 41.0 60-61 36.079195096683556 39.0 35.5 41.0 28.5 41.0 62-63 35.3317825519464 38.5 34.5 40.0 27.0 41.0 64-65 34.23442195856377 37.5 32.5 39.5 26.0 41.0 66-67 33.72853001707594 36.0 32.0 39.0 25.0 40.0 68-69 33.890712346410744 37.0 33.5 39.0 26.0 39.0 70-71 33.25889152737288 36.0 32.5 38.0 25.5 39.0 72-73 32.15939281558636 35.0 32.0 37.0 23.0 38.0 74-75 31.806828119092316 35.0 32.0 36.5 23.0 37.0 76-77 31.014563780342925 34.5 30.5 36.0 21.5 37.0 78-79 30.319773676179203 34.0 30.0 35.0 19.0 36.0 80-81 30.23364196320101 34.0 30.5 35.0 18.0 36.0 82-83 30.31664995502434 34.0 31.0 35.0 18.0 36.0 84-85 30.011623380994457 34.0 31.0 35.0 14.5 35.5 86-87 29.292869636520635 34.0 29.0 35.0 4.5 35.0 88-89 28.408032135808945 33.5 28.0 35.0 2.0 35.0 90-91 27.68921641910082 33.0 26.0 35.0 2.0 35.0 92-93 27.664718382750593 33.0 26.5 35.0 2.0 35.0 94-95 27.650525672608623 34.0 28.0 35.0 2.0 35.0 96-97 26.597857210782372 33.0 25.0 35.0 2.0 35.0 98-99 25.823116131845776 33.0 24.0 35.0 2.0 35.0 100-101 23.662774423662036 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15780178196860817 1101 2 0.03538579796785868 1101 3 0.2319288426994497 1101 4 0.2213992640485145 1101 5 0.011689502913171168 1101 6 0.0018625199641419954 1101 7 0.14184278570893127 1101 8 0.1687209342306133 1101 9 0.31377562532121317 1101 10-11 0.30068881751557086 1101 12-13 0.24040737258209077 1101 14-15 0.4231692786948855 1101 16-17 0.44157805811800444 1101 18-19 0.47646029853076044 1101 20-21 0.5275033852436692 1101 22-23 0.5108624627323977 1101 24-25 0.4940127013454543 1101 26-27 0.480988599930086 1101 28-29 0.3745702110943441 1101 30-31 0.35641084862130157 1101 32-33 0.41742052772796967 1101 34-35 0.41942309708367276 1101 36-37 0.5962682720040817 1101 38-39 0.6023109524083736 1101 40-41 0.5193808311718371 1101 42-43 0.4757037674644735 1101 44-45 0.5957058398579207 1101 46-47 0.652362579841089 1101 48-49 0.6431524232860184 1101 50-51 0.5931906403197473 1101 52-53 0.5938142753875724 1101 54-55 0.5865677567331105 1101 56-57 0.6896725575233944 1101 58-59 0.7308406386651356 1101 60-61 0.5487321247407735 1101 62-63 0.5037202975471047 1101 64-65 0.47977345569533014 1101 66-67 0.3572230026499241 1101 68-69 0.4726545904167878 1101 70-71 0.39973025919750427 1101 72-73 0.4727486994542147 1101 74-75 0.3509447923644977 1101 76-77 0.2368111465616991 1101 78-79 0.2684297927347252 1101 80-81 0.33092049063579054 1101 82-83 0.3095656551694752 1101 84-85 0.36838429510990167 1101 86-87 0.4137879692029429 1101 88-89 0.2560522101442473 1101 90-91 0.13853643945433802 1101 92-93 0.007337268749747494 1101 94-95 0.15776188521887136 1101 96-97 0.04124278431757844 1101 98-99 0.02524202163055378 1101 100-101 0.2828696730034146 1102 1 0.18048273757344901 1102 2 0.16732622988329027 1102 3 0.23480099630332418 1102 4 0.23063128121330578 1102 5 0.21312644300143546 1102 6 0.23869224038886472 1102 7 0.22268423753074984 1102 8 0.1999775769698644 1102 9 0.3212791108397539 1102 10-11 0.2302171112268283 1102 12-13 0.2596200587738551 1102 14-15 0.24203047453902826 1102 16-17 0.31414750037195205 1102 18-19 0.39222923596142323 1102 20-21 0.33088708580019244 1102 22-23 0.35637272637672623 1102 24-25 0.42106935650274124 1102 26-27 0.4163338497136877 1102 28-29 0.39025849766024834 1102 30-31 0.39261664545615815 1102 32-33 0.37558924176669706 1102 34-35 0.5104803804477811 1102 36-37 0.5385567676844971 1102 38-39 0.4615155602323284 1102 40-41 0.433462608293226 1102 42-43 0.36538952128111646 1102 44-45 0.5842667813978366 1102 46-47 0.5570353162960444 1102 48-49 0.5599748356599648 1102 50-51 0.4451721480625963 1102 52-53 0.47943386760431395 1102 54-55 0.43837883183336857 1102 56-57 0.43056535163923826 1102 58-59 0.40349821913255113 1102 60-61 0.47333515414529614 1102 62-63 0.4380046016403796 1102 64-65 0.36003776740543714 1102 66-67 0.31929624452581606 1102 68-69 0.26763282065990524 1102 70-71 0.2826835749972716 1102 72-73 0.28044009916342105 1102 74-75 0.2591359020426971 1102 76-77 0.3117960805551583 1102 78-79 0.1692247798302553 1102 80-81 0.13478251822310128 1102 82-83 0.15434868309313643 1102 84-85 0.17152624229205315 1102 86-87 0.1240592336883104 1102 88-89 0.08565879728453751 1102 90-91 -0.01490438741458533 1102 92-93 -0.09074064084889955 1102 94-95 -0.05954554465488471 1102 96-97 -0.09507339695528216 1102 98-99 -0.20706406184856974 1102 100-101 0.01938173726378878 1103 1 0.06362774734544985 1103 2 0.11912913266156622 1103 3 0.1067644299048105 1103 4 0.1205282119732729 1103 5 0.07438581066269023 1103 6 0.14630502931819933 1103 7 0.0868947505100337 1103 8 0.13681571938711556 1103 9 0.15944753426755653 1103 10-11 0.16028551890168075 1103 12-13 0.22770779732535118 1103 14-15 0.36907055031067415 1103 16-17 0.2704353095497396 1103 18-19 0.3699231765445816 1103 20-21 0.3598327061870563 1103 22-23 0.41086971659843385 1103 24-25 0.35964597528639786 1103 26-27 0.37215781857435104 1103 28-29 0.3905508210529902 1103 30-31 0.3374266970115727 1103 32-33 0.3199621418830745 1103 34-35 0.33947618100599897 1103 36-37 0.40040344943135153 1103 38-39 0.3130728141947685 1103 40-41 0.38598061741151213 1103 42-43 0.4159469092118826 1103 44-45 0.4730960458971296 1103 46-47 0.40293803687549 1103 48-49 0.463451991615635 1103 50-51 0.5470816205947813 1103 52-53 0.5244732466390616 1103 54-55 0.44670323738505857 1103 56-57 0.4134293772194084 1103 58-59 0.519369515423584 1103 60-61 0.536623200427023 1103 62-63 0.5110925943792282 1103 64-65 0.21746055724324265 1103 66-67 0.18862443322993983 1103 68-69 0.17320823684048747 1103 70-71 0.2310638236525122 1103 72-73 0.3898473523590127 1103 74-75 0.25518358244577755 1103 76-77 0.28371201398683965 1103 78-79 0.3001398393718553 1103 80-81 0.3159795034499737 1103 82-83 0.4171721015691219 1103 84-85 0.4323032223742125 1103 86-87 0.41476898923374605 1103 88-89 0.32233298965384094 1103 90-91 0.16189439454375787 1103 92-93 0.1782517982760865 1103 94-95 0.20436683085245377 1103 96-97 0.19425153764267833 1103 98-99 0.2793370577316061 1103 100-101 0.4163767505874283 1104 1 0.10268033628159401 1104 2 0.1562727893358158 1104 3 0.07848462908329523 1104 4 0.17233160570025774 1104 5 0.14335301570410763 1104 6 0.11629830066051028 1104 7 0.10901989356511876 1104 8 0.13408565300127862 1104 9 0.14424050628666407 1104 10-11 0.06165148599662729 1104 12-13 0.1915331932975306 1104 14-15 0.22154333885423227 1104 16-17 0.2650790923920212 1104 18-19 0.25971000564814517 1104 20-21 0.2576311370916997 1104 22-23 0.34328955380926374 1104 24-25 0.22301741577734902 1104 26-27 0.2426736856763796 1104 28-29 0.3037683091625425 1104 30-31 0.32169152765726494 1104 32-33 0.24206368489912933 1104 34-35 0.24070951575996702 1104 36-37 0.3231088042218033 1104 38-39 0.3734264765068076 1104 40-41 0.21728300281329638 1104 42-43 0.3707448187436668 1104 44-45 0.5342813981639125 1104 46-47 0.490631164089649 1104 48-49 0.500105136780185 1104 50-51 0.5507945504258629 1104 52-53 0.5325538415936748 1104 54-55 0.5595231052960159 1104 56-57 0.5213454381045821 1104 58-59 0.5502793760206046 1104 60-61 0.520576968686747 1104 62-63 0.5551080664893675 1104 64-65 0.37417864236564213 1104 66-67 0.48802156144164144 1104 68-69 0.451051225519258 1104 70-71 0.5406537928997608 1104 72-73 0.5015833463457753 1104 74-75 0.40779377626938995 1104 76-77 0.27126249146783366 1104 78-79 0.38709338918368275 1104 80-81 0.31774710317603194 1104 82-83 0.40054701787918034 1104 84-85 0.43644440864943235 1104 86-87 0.37238903207338225 1104 88-89 0.338580040751701 1104 90-91 0.22141709698732726 1104 92-93 0.23458759805381746 1104 94-95 0.2697042750582703 1104 96-97 0.05893628167153864 1104 98-99 0.14793384741446758 1104 100-101 0.4503065978743699 1105 1 0.06149863963958779 1105 2 0.03397427049348778 1105 3 0.08163477447562428 1105 4 0.11780322976856183 1105 5 0.08735745787872418 1105 6 0.09892183772586094 1105 7 0.06067995593242159 1105 8 0.03097937818961327 1105 9 0.15376890376181507 1105 10-11 0.09570646160900509 1105 12-13 0.04602827663182296 1105 14-15 0.11771641363019114 1105 16-17 0.05932297493870209 1105 18-19 0.0489775225267195 1105 20-21 0.09544994884642932 1105 22-23 0.17226390786766643 1105 24-25 0.137484386776471 1105 26-27 0.10916033577280615 1105 28-29 0.14107163942571788 1105 30-31 0.14058157623009748 1105 32-33 0.23698866753640146 1105 34-35 0.16560021017391335 1105 36-37 0.11939883829545295 1105 38-39 0.18468366287719817 1105 40-41 0.07847174026274928 1105 42-43 0.0980697563739028 1105 44-45 0.20591420819938122 1105 46-47 0.2506989938891522 1105 48-49 0.2052344263107173 1105 50-51 0.3019370694913164 1105 52-53 0.2859584528367378 1105 54-55 0.3384329894671012 1105 56-57 0.22663273228393876 1105 58-59 0.2691283214075284 1105 60-61 0.39677447056499915 1105 62-63 0.2765904034116673 1105 64-65 0.17868120229253748 1105 66-67 0.10711377730964955 1105 68-69 0.08698831584109001 1105 70-71 0.14027806628875084 1105 72-73 0.1770757033191117 1105 74-75 0.047558806815846566 1105 76-77 0.1279975017961128 1105 78-79 0.10649062284731414 1105 80-81 0.17895443451076787 1105 82-83 0.2534826439108606 1105 84-85 0.13145772814636913 1105 86-87 0.19677402196718674 1105 88-89 0.10548087759509883 1105 90-91 0.1502403547626905 1105 92-93 0.2041865377801635 1105 94-95 0.46686959820828733 1105 96-97 0.42806012594083853 1105 98-99 0.5168484743275812 1105 100-101 0.7702667491250743 1106 1 0.08931399164804077 1106 2 0.11566699058510821 1106 3 0.07652104641031698 1106 4 0.11692436096160463 1106 5 0.024815174757677028 1106 6 0.019213694036196216 1106 7 0.01847767076832696 1106 8 0.0864113421943884 1106 9 0.12340054957217461 1106 10-11 0.1484757274724231 1106 12-13 0.12387428814208334 1106 14-15 0.14065916105929688 1106 16-17 0.05574777250985363 1106 18-19 0.12844515176794147 1106 20-21 0.14626219725511191 1106 22-23 0.08398046070430354 1106 24-25 0.052150356002911735 1106 26-27 -0.11118054805688615 1106 28-29 0.07228814755346491 1106 30-31 0.033350861471987514 1106 32-33 0.0579289826911662 1106 34-35 0.1074591642848759 1106 36-37 0.21452736874054779 1106 38-39 0.18827644434318813 1106 40-41 0.1296805689811933 1106 42-43 0.24342188663484166 1106 44-45 0.19291660033261593 1106 46-47 0.11508796178373615 1106 48-49 0.21205161844294906 1106 50-51 0.2598004634857034 1106 52-53 0.2384797158587233 1106 54-55 0.1427507055100108 1106 56-57 0.16514357190581563 1106 58-59 0.09459727336747648 1106 60-61 0.24162968158680798 1106 62-63 -0.010064657399347254 1106 64-65 -0.04254289665723121 1106 66-67 -0.14040053299551403 1106 68-69 0.17814826099449732 1106 70-71 0.0761148298412877 1106 72-73 -0.024922950154326884 1106 74-75 -0.05400653031271574 1106 76-77 -0.0769013329213486 1106 78-79 0.1774345645435993 1106 80-81 0.0654255106274455 1106 82-83 0.12887351449399276 1106 84-85 0.19586696037984197 1106 86-87 0.2853326710334052 1106 88-89 0.24356846383273378 1106 90-91 0.1709089146132179 1106 92-93 0.3166722911470998 1106 94-95 0.3848998352595281 1106 96-97 0.4706353912112782 1106 98-99 0.6452913841205437 1106 100-101 0.8753689193194951 1107 1 0.018417869769127293 1107 2 0.06392350671373137 1107 3 0.02810099679998146 1107 4 0.08542748589660931 1107 5 0.003191840996926487 1107 6 0.06072663282317592 1107 7 -0.13407384385818943 1107 8 4.6490039701296837E-4 1107 9 -0.09148889615963895 1107 10-11 -0.05636162240576681 1107 12-13 0.05866452900313135 1107 14-15 0.04389565480244784 1107 16-17 0.04831526660345986 1107 18-19 0.17480535354787463 1107 20-21 0.0874873529226079 1107 22-23 0.12008578879943599 1107 24-25 -0.05913188762063726 1107 26-27 0.06412631747941333 1107 28-29 -0.1603230116385177 1107 30-31 0.014596327834674128 1107 32-33 0.10289745161387742 1107 34-35 0.04838313503895364 1107 36-37 0.12647076314375738 1107 38-39 0.13302831236632073 1107 40-41 0.08764036570573097 1107 42-43 0.16684241676136224 1107 44-45 0.18247489142220985 1107 46-47 0.1802439294393352 1107 48-49 0.2262587970125125 1107 50-51 0.33961268829145297 1107 52-53 0.27873164852115906 1107 54-55 0.23573095642634456 1107 56-57 0.15124732022170662 1107 58-59 0.09068218544882001 1107 60-61 0.0038061460882445886 1107 62-63 -0.08314228327549955 1107 64-65 -0.03527219964019679 1107 66-67 0.02089232147864095 1107 68-69 0.14240363599591177 1107 70-71 0.1351529443852897 1107 72-73 0.04337692301572815 1107 74-75 0.13960363124867925 1107 76-77 -0.007981554374804745 1107 78-79 0.0033566487779559395 1107 80-81 0.024118245933589577 1107 82-83 0.08710228180014568 1107 84-85 -0.03432567526855834 1107 86-87 0.0019845974820462686 1107 88-89 -0.02704612821955621 1107 90-91 -0.011230298693575946 1107 92-93 0.10391469168486012 1107 94-95 0.04324083271634649 1107 96-97 0.13000020410354196 1107 98-99 0.21982507936374773 1107 100-101 0.44636581794953756 1108 1 0.016406034204131004 1108 2 -0.06736986125319433 1108 3 -0.11399541417837611 1108 4 -0.02300237434445762 1108 5 -0.024202286156466357 1108 6 0.03783639852264997 1108 7 0.05912771832478114 1108 8 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-4.059129825416523 2316 100-101 -4.207298871204628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17575.0 3 5342.0 4 4130.0 5 4269.0 6 5054.0 7 5725.0 8 7273.0 9 9249.0 10 11442.0 11 15339.0 12 19706.0 13 25869.0 14 27292.0 15 20618.0 16 23084.0 17 26194.0 18 28347.0 19 29443.0 20 33845.0 21 38716.0 22 44677.0 23 51891.0 24 60734.0 25 72595.0 26 86650.0 27 101556.0 28 122740.0 29 147096.0 30 176497.0 31 215240.0 32 267670.0 33 338949.0 34 444427.0 35 619197.0 36 963451.0 37 1599229.0 38 865322.0 39 14884.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.19623285582173 8.786787430458974 6.207182075067999 12.809797638651304 2 7.503193496801461 26.36950633374956 34.66861044350226 31.458689725946716 3 32.63253127454841 25.666655157905797 35.61664093491063 6.084172632635159 4 6.308281197620453 10.381925865170675 62.50797200455081 20.801820932658067 5 9.590117360395169 59.689239625554414 22.483546101328447 8.237096912721976 6 13.030844652863088 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22.035179487492858 28-29 19.611819491878308 18.48892132670958 48.50278971707506 13.396469464337049 30-31 12.84295683297316 44.036233928271685 31.380973101996872 11.739836136758283 32-33 16.05931147304646 40.993520472180236 14.073348719182993 28.873819335590305 34-35 24.088836791085214 19.075700627901178 22.414910783319176 34.420551797694436 36-37 37.189453274905865 20.445997746211308 29.632567422931338 12.731981555951489 38-39 41.03669739328531 10.029420375144854 30.118441176229343 18.815441055340493 40-41 20.098004652918007 18.3838620568301 50.13662466941688 11.381508620835005 42-43 18.160622514376247 38.298267720954065 27.27232305456016 16.268786710109527 44-45 11.745760507953955 42.19740564186106 20.830330462026122 25.22650338815886 46-47 15.783169903797662 24.04578531509375 10.645424634907531 49.525620146201064 48-49 24.44258430680636 27.09755674687181 14.908114508784742 33.55174443753709 50-51 39.86755609724381 27.00722231695633 22.01554596900927 11.109675616790591 52-53 19.888790748143542 25.330891461404985 35.61795429173382 19.162363498717657 54-55 10.626228528049639 32.89110348836219 41.54951731407512 14.933150669513045 56-57 16.42854542046862 42.684911177401055 25.69765476450101 15.188888637629322 58-59 18.103865432199626 26.169084666182407 24.11785335413225 31.609196547485713 60-61 18.251825307415356 18.262681841194578 30.355443069164316 33.13004978222575 62-63 43.91459564703535 11.803491426823138 22.141738396963458 22.140174529178054 64-65 37.51974935554331 16.649927996622 34.12296756680731 11.707355081027382 66-67 19.736357541549836 22.748075410206695 40.239705777918346 17.275861270325127 68-69 10.20574943020804 38.96284057513937 33.299488912948306 17.531921081704287 70-71 15.667480115898492 38.55710243339894 14.543576443136095 31.231841007566473 72-73 17.603994707221528 29.615647777157278 12.512417890842809 40.267939624778386 74-75 19.585395002762525 26.90855217223434 24.572027141378793 28.93402568362434 76-77 34.51924687716231 18.58824927499564 24.0880700604346 22.804433787407447 78-79 19.17430819257067 19.326672425101574 46.120970516777504 15.378048865550253 80-81 14.457712188730149 40.76917758331229 30.55425882940199 14.218851398555568 82-83 16.938244797423994 37.06129384340022 16.61103856642257 29.389422792753212 84-85 22.95711193850898 17.891166277329017 24.35119165531567 34.80053012884633 86-87 34.19426774471116 18.71666171254019 29.649285413577047 17.439785129171597 88-89 37.16977335753724 11.707665007696248 30.178725908877112 20.9438357258894 90-91 19.92259853833024 18.840014818065004 45.355762769558 15.881623874046754 92-93 18.59942544015068 35.02930963788374 28.213736632028407 18.157528289937165 94-95 14.237138478747049 38.3313160788868 22.400596441672054 25.030949000694097 96-97 16.622113564015592 23.850743910790523 15.150480794670498 44.37666173052339 98-99 22.795336173107792 26.36465952344455 18.368781692587678 32.47122261085998 100-101 35.21274178095047 25.11973271144294 23.8085537122661 15.858971795340485 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2672.0 1 1425.0 2 211.5 3 239.0 4 215.0 5 229.5 6 299.5 7 442.5 8 651.0 9 816.5 10 908.5 11 1068.5 12 1443.5 13 2214.0 14 4513.0 15 6947.5 16 6654.5 17 4445.0 18 2733.0 19 2217.0 20 2168.0 21 2218.0 22 2384.5 23 2675.0 24 2963.0 25 3385.5 26 4011.5 27 4654.5 28 5178.0 29 5703.0 30 6390.5 31 7128.5 32 7925.0 33 8744.0 34 9654.5 35 10443.0 36 11449.0 37 12693.5 38 13229.5 39 13472.5 40 15230.0 41 22809.0 42 60339.0 43 183484.0 44 569854.0 45 1118896.0 46 1414415.0 47 1272019.5 48 843441.5 49 423202.5 50 176052.0 51 80352.5 52 47223.5 53 32988.5 54 24318.5 55 17874.5 56 13570.5 57 10775.5 58 9093.5 59 8052.0 60 6857.0 61 5554.0 62 4488.0 63 3558.0 64 2695.5 65 2037.5 66 1516.5 67 1214.5 68 924.5 69 622.0 70 455.5 71 350.5 72 312.0 73 289.0 74 227.5 75 172.0 76 146.0 77 97.0 78 64.0 79 53.0 80 36.0 81 30.5 82 24.5 83 19.5 84 18.5 85 15.5 86 11.0 87 8.0 88 7.0 89 8.5 90 8.5 91 4.0 92 2.5 93 4.0 94 5.0 95 3.5 96 2.0 97 2.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1587160818405937 2 0.09140141354697777 3 0.0852194542138906 4 0.09987298744787501 5 0.12038793397545319 6 0.03870975029310882 7 0.04202205936787405 8 0.046036516910101036 9 0.04614336558993217 10-11 0.04470854046077119 12-13 0.04886037487706678 14-15 0.04662418464917228 16-17 0.04539542483111421 18-19 0.049593051538765996 20-21 0.05162317645555759 22-23 0.06491820504597469 24-25 0.08046468796140503 26-27 0.08335723436540508 28-29 0.07294712013042866 30-31 0.06154483958273454 32-33 0.05056995375436497 34-35 0.29249826103773574 36-37 0.46252503884331114 38-39 0.43961362906809176 40-41 0.41583979780566394 42-43 0.4455666269443979 44-45 0.45464113268148515 46-47 0.45416794567080443 48-49 0.471591912531839 50-51 0.4601362076442293 52-53 0.46769956776656185 54-55 0.45870901456362767 56-57 0.43698820436366953 58-59 0.4161603438451573 60-61 0.38613586481260803 62-63 0.44312437140540056 64-65 0.48063589007468877 66-67 0.4826507508943616 68-69 0.49264873450713226 70-71 0.49101547611542773 72-73 0.4807274746574011 74-75 0.47346939647744324 76-77 0.4549998389637754 78-79 0.42947826858125254 80-81 0.43478254233001257 82-83 0.4470472443649151 84-85 0.44468894136007076 86-87 0.455198272226319 88-89 0.46723401280444055 90-91 0.47168349711455143 92-93 0.47357624515727437 94-95 0.4780791538073008 96-97 0.4790789521685779 98-99 0.47269092752438785 100-101 0.45989198209032955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6551321.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.151697251886848 #Duplication Level Percentage of deduplicated Percentage of total 1 77.99201998495701 9.47735414920306 2 10.08096189638612 2.450015939453824 3 3.427079391855977 1.2493449368404297 4 1.806926012207418 0.8782887142761496 5 1.1456711402582194 0.6960924423320937 6 0.7871176719640129 0.5738889390790002 7 0.5990904992403397 0.5095976461275249 8 0.4508148867426156 0.4382532816271938 9 0.35576988382551933 0.3890887127628 >10 2.518906611567974 6.143080204008116 >50 0.38748346262296074 3.3168945842909663 >100 0.32519046108974287 8.28694042507593 >500 0.06609693204375905 5.632987359887721 >1k 0.043601226682785606 9.313332517755661 >5k 0.005307975422252161 4.450839246737787 >10k+ 0.007961963133378241 46.19400090054176 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 368975 5.63207023438479 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 237709 3.628413261997084 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 220583 3.3670003347416495 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 212871 3.249283617761975 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 174481 2.663294929373786 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 144048 2.198762661759361 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 124153 1.895083449582153 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 121882 1.8604186850255087 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 114690 1.7506392985475752 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 112567 1.7182336203645034 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 99835 1.5238911358487852 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 98285 1.500231785314748 No Hit CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 62348 0.9516859271588127 No Hit CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA 37362 0.5702971965501309 No Hit GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT 36030 0.5499654191879775 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 35127 0.5361819394897609 No Hit CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 34659 0.5290383420381936 No Hit GCTTTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 33274 0.5078975675287473 No Hit GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG 31111 0.4748813254609261 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 30425 0.4644101548374748 No Hit GAGTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 29420 0.4490697372331473 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA 28638 0.4371332132862975 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 25409 0.3878454436899062 No Hit TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC 22681 0.3462049867500005 No Hit ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA 21961 0.3352148368245122 No Hit TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG 20944 0.31969125005475996 No Hit CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 20833 0.3179969352745805 No Hit GCTTTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 20569 0.3139672136352348 No Hit GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT 20367 0.31088386601725054 No Hit GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT 19987 0.3050835091121318 No Hit GCTTATACTCTGCGTTGATACCACTGCTTTTACTCTGCGTTGATACCACT 19566 0.29865732422514485 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT 19172 0.2926432699603637 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 18819 0.2872550436774507 No Hit AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG 18125 0.2766617602770495 No Hit GAGTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17727 0.2705866496237934 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG 17012 0.25967282018389876 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC 16277 0.24845370880162945 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 16264 0.24825527553908594 No Hit GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 16248 0.2480110499851862 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 15828 0.241600129195318 No Hit GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCA 15805 0.24124905496158713 No Hit GTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 14875 0.22705344464116475 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG 14399 0.21978773441264748 No Hit ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTAT 13682 0.20884337677851533 No Hit CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAG 13135 0.20049391565456798 No Hit TGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 12521 0.19112176002366546 No Hit GTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCAGA 12465 0.19026697058501638 No Hit CCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATA 12172 0.18579459012922736 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG 11706 0.17868152087189743 No Hit ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 11520 0.17584239880781297 No Hit TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 11236 0.17150739522609257 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA 11215 0.17118684918659915 No Hit AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAT 11141 0.17005730599981286 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA 11106 0.16952306260065717 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 11095 0.1693551575323511 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 11023 0.16825614253980228 No Hit GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 10733 0.1638295543753695 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGT 10652 0.16259316250875205 No Hit ATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG 10428 0.1591740047541557 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 10390 0.15859396906364379 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 10234 0.15621276991312133 No Hit AGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 10077 0.15381630666548013 No Hit GTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGC 10067 0.1536636656942928 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA 9646 0.1472374808073059 No Hit GATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTT 9551 0.1457873915810262 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG 9292 0.14183399042727413 No Hit GCTTCTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 9188 0.1402465243269258 No Hit GTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGCTT 8855 0.13516357998638748 No Hit GTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8852 0.13511778769503127 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 8799 0.13430879054773837 No Hit GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCA 8512 0.1299279946746618 No Hit GCTTACACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 8215 0.12539455783039788 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCA 7916 0.12083059279189648 No Hit TGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGT 7898 0.12055583904375927 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 7781 0.11876993968086742 No Hit ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGT 7654 0.11683139934678823 No Hit CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAG 7608 0.11612925087932646 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTAT 7511 0.11464863345880931 No Hit TACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGA 7480 0.11417544644812855 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAG 7457 0.11382437221439767 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 7395 0.11287799819303618 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7394 0.11286273409591745 No Hit ATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGT 7211 0.11006940432318918 No Hit CCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 6853 0.10460485755468248 No Hit GAGTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGC 6570 0.10028511807008082 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.5264097118733765E-5 0.0 9 0.0 0.0 0.0 1.3737687406860388E-4 0.0 10-11 0.0 0.0 0.0 2.7475374813720776E-4 0.0 12-13 0.0 0.0 0.0 4.4265881644327916E-4 0.0 14-15 0.0 0.0 0.0 5.418754477150487E-4 0.0 16-17 0.0 0.0 0.0 8.547894386490907E-4 0.0 18-19 0.0 0.0 0.0 0.0011524393324643992 0.0 20-21 0.0 0.0 0.0 0.0017095788772981816 0.0 22-23 0.0 0.0 0.0 0.003213092443493457 0.0 24-25 0.0 0.0 0.0 0.005060048194860243 0.0 26-27 0.0 0.0 0.0 0.00695279623758323 0.0 28-29 0.0 0.0 0.0 0.012669200608549024 0.0 30-31 0.0 0.0 0.0 0.033497061127061245 0.0 32-33 0.0 0.0 0.0 0.08243375648972169 0.0 34-35 0.0 0.0 0.0 0.3577904364631194 0.0 36-37 0.0 0.0 0.0 0.7886882660764142 0.0 38-39 0.0 0.0 0.0 0.9378032308293244 0.0 40-41 0.0 0.0 0.0 1.0713030242297696 0.0 42-43 0.0 0.0 0.0 1.2392767809728755 0.0 44-45 0.0 0.0 0.0 1.4277654842435594 0.0 46-47 0.0 0.0 0.0 1.732162108985348 0.0 48-49 0.0 0.0 0.0 2.0949210090606156 0.0 50-51 0.0 0.0 0.0 2.391128445698204 0.0 52-53 0.0 0.0 0.0 2.595278112612708 0.0 54-55 0.0 0.0 0.0 2.8074490625631077 0.0 56-57 0.0 0.0 0.0 3.0166282494782353 0.0 58-59 0.0 0.0 0.0 3.887353405519284 0.0 60-61 0.0 0.0 0.0 5.749344292548022 0.0 62-63 0.0 0.0 0.0 6.871507288377413 0.0 64-65 0.0 0.0 0.0 7.215956903958759 0.0 66-67 0.0 0.0 0.0 7.643328727137627 0.0 68-69 0.0 0.0 0.0 8.018657611190172 0.0 70-71 7.632048559366883E-6 0.0 0.0 8.529478253317155 0.0 72-73 2.2896145678100648E-5 0.0 0.0 9.311564797389718 0.0 74-75 3.052819423746753E-5 0.0 0.0 9.985558637715966 0.0 76-77 3.052819423746753E-5 0.0 0.0 10.46637006490752 0.0 78-79 3.052819423746753E-5 0.0 0.0 10.900900749635074 0.0 80-81 3.052819423746753E-5 0.0 0.0 11.39434321719238 0.0 82-83 3.052819423746753E-5 0.0 0.0 11.982819953410923 0.0 84-85 3.052819423746753E-5 0.0 0.0 14.725480555753565 0.0 86-87 3.052819423746753E-5 0.0 0.0 18.104905865549863 0.0 88-89 3.052819423746753E-5 0.0 0.0 18.904156886832443 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATC 810 0.0 31.703775 94-95 ACCACTC 3140 0.0 29.835876 94-95 CTTAGGG 3925 0.0 29.078619 94-95 GCCGGAT 455 0.0 27.174664 94-95 TACGTTA 70 0.008497047 27.075304 4 GCCGTAT 1455 0.0 26.801128 94-95 GCCGTAG 125 0.0 26.631172 94-95 GCTTCCC 2395 0.0 26.524855 1 CGTATCG 45 8.8915287E-4 26.419811 94-95 TACCTGG 700 0.0 26.398424 2 TTATGTA 615 0.0 26.194807 3 CGTATCC 75 1.0507392E-6 25.36302 94-95 ACACTCT 7570 0.0 25.161983 5 GCTTACA 8470 0.0 24.963484 1 CTTCCCA 2535 0.0 24.48526 2 TACACTC 7915 0.0 24.30449 4 GCGCCGT 520 0.0 24.235283 92-93 CTTACAC 8175 0.0 23.99518 2 CGTATCT 110 2.1445885E-9 23.77783 94-95 GTACTAA 180 1.9907748E-7 23.704113 1 >>END_MODULE