FastQCFastQC Report
Thu 26 May 2016
SRR938861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6551321
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT3816135.824977893771348No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA2305963.5198397391915313No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2299923.5106202245318157No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA2113293.2257463800048876No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA1819392.7771345656853024No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1494972.2819367269593416No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1204121.83798046226097No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1128461.7224923034606303No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1113651.6998861756277857No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1108591.6921625424857063No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA977521.4920960215504628No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT914401.3957490405370154No Hit
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG676911.0332419980642071No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA387240.5910868968258463No Hit
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG386030.5892399410744794No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT375810.5736400338191336No Hit
CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG370570.565641646928917No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT350240.5346097374865313No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG322440.4921755474964515No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA304920.46543284934442997No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG296940.45325209984368037No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA293900.44861181431958536No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA259670.3963628098821596No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC246250.37587839154881897No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA229660.3505552544288396No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG229110.34971572908730925No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT222730.3399772351255571No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA213750.3262700759129342No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT209240.3193859681123853No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT207950.3174168995840686No Hit
GCTTATACTCTGCGTTGATACCACTGCTTTTACTCTGCGTTGATACCACT204220.3117233913587809No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG201750.3079531593704537No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT197960.3021680665624536No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT191790.29275011864019485No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA181730.27739443693874866No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC174390.2661905896535981No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC171790.2622219244027273No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA166680.2544219707750544No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG165530.2526665996064No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCA161710.2468357145070437No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG161160.24599618916551336No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG159600.2436149900149909No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG151420.23112895857186666No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTAT146130.22305425119605649No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC137270.20953026114885837No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAG135170.2063248007539243No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG129910.19829588566947035No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATA124020.18930533246653614No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG122890.18758048949211922No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG121530.18550457228397144No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA119710.18272650660836187No Hit
GTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCAGA118050.18019266648665208No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG117540.17941419753359666No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA116130.1772619598398552No Hit
AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAT114490.17475864791238288No Hit
ATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG112080.17108000050676803No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG111450.1701183623882878No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGT108150.16508121033910567No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA108090.16498962575639325No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA107900.1646996079111373No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGT106710.162883180354008No Hit
GATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTT103030.15726599261431398No Hit
GTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGC101590.1550679626292163No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT101120.15435055006463583No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA100000.15264097118733763No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG99240.15148089980631388No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA99120.15129773064088906No Hit
GCTTCTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT95340.1455279019300077No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG94970.14496313033661457No Hit
TGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGT87720.13389665992553257No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCA86820.13252289118484653No Hit
GCTTACACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT86750.1324160425050154No Hit
GTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGCTT82900.12653936511430292No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGT81690.12469240936293612No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCA81370.12420395825513664No Hit
ATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGT81260.12403605318683057No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTAT81110.12380709173004956No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA79580.12147168487088328No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAG78150.11928891898290436No Hit
TACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGA77660.1185409782240864No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT76970.11748775552289378No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAG76640.11698404031797557No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG76630.11696877622085684No Hit
AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAC68670.10481855491434476No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA68500.10455906526332628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTC59650.038.06592694-95
TATGTAC7400.035.2689554
TTATGTA6900.034.3860663
CTTATGT7500.032.2678872
GCGCTTA1201.4712896E-731.7549061
TACGCTT1100.030.19744994-95
GCGTTAG650.00576709729.3122221
GCGTCAG1004.863483E-528.5794161
CCAAGAC2450.027.115873
TATAGGG909.0041955E-426.362652
CAACGGC5050.026.31044818-19
GCTTACA87250.025.658691
ACACTCT80700.025.6374345
TACACTC82150.025.3582084
GCTTCCC25800.025.108531
CTTACAC85950.024.7891752
TACCTGG6900.024.7579672
GTACCCG1351.3153131E-524.6982611
TTACACT85050.024.6609343
TAAACTC13300.024.6183554
Produced by FastQC (version 0.11.5)