##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938861_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6551321 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.954239457965805 31.0 31.0 34.0 30.0 34.0 2 32.042614916899964 33.0 31.0 34.0 30.0 34.0 3 32.25744899387467 34.0 31.0 34.0 30.0 34.0 4 35.59356334394239 37.0 35.0 37.0 33.0 37.0 5 35.600425166161145 37.0 35.0 37.0 33.0 37.0 6 35.71851142693207 37.0 35.0 37.0 33.0 37.0 7 35.78649054137326 37.0 35.0 37.0 33.0 37.0 8 35.809010121775444 37.0 35.0 37.0 33.0 37.0 9 37.36818314962738 39.0 37.0 39.0 34.0 39.0 10-11 37.20264600070734 39.0 37.0 39.0 33.5 39.0 12-13 37.04083611534223 39.0 37.0 39.0 32.5 39.0 14-15 37.79471628699006 39.0 37.0 41.0 32.5 41.0 16-17 37.64170874851041 39.0 36.5 40.5 32.5 41.0 18-19 38.310080821867835 40.0 37.5 41.0 33.5 41.0 20-21 38.508586359911234 40.0 38.0 41.0 34.0 41.0 22-23 38.50026193190656 40.0 38.0 41.0 34.0 41.0 24-25 38.48927277414738 40.0 38.0 41.0 33.5 41.0 26-27 37.82740839595556 39.5 37.0 41.0 32.5 41.0 28-29 37.44271506464116 39.0 36.5 40.5 31.5 41.0 30-31 37.71005565137169 39.5 37.0 41.0 32.5 41.0 32-33 37.98922072357621 40.0 37.5 41.0 33.0 41.0 34-35 38.26927660848858 40.0 38.0 41.0 33.5 41.0 36-37 37.82670991087141 40.0 37.0 41.0 33.0 41.0 38-39 37.246795951534054 39.0 36.5 41.0 30.5 41.0 40-41 36.90476874816545 39.0 35.5 40.0 30.5 41.0 42-43 37.42491254817158 39.0 37.0 40.5 32.0 41.0 44-45 37.81586797532894 40.0 37.0 41.0 33.0 41.0 46-47 37.45816088083609 40.0 37.0 41.0 31.5 41.0 48-49 37.52157129836868 40.0 37.0 41.0 31.5 41.0 50-51 37.45834328679666 40.0 37.0 41.0 32.0 41.0 52-53 36.686208705084056 39.0 35.5 41.0 30.0 41.0 54-55 36.28458909279518 38.5 35.0 40.0 29.0 41.0 56-57 36.85272077188708 39.0 36.0 40.0 31.0 41.0 58-59 37.026324614531944 39.0 36.0 41.0 31.0 41.0 60-61 36.576340176278954 39.0 35.5 40.5 29.5 41.0 62-63 35.6930970868318 38.0 34.5 40.0 28.5 41.0 64-65 34.53671175630075 37.0 32.0 39.0 26.0 40.5 66-67 34.116040642795554 36.0 32.5 39.0 26.5 39.5 68-69 34.455751519426386 36.0 34.0 39.0 28.0 39.0 70-71 33.81650059583403 36.0 33.5 38.0 27.0 39.0 72-73 32.86040807647801 35.0 32.0 37.0 26.0 37.5 74-75 32.512210285528674 35.0 32.0 36.5 26.0 37.0 76-77 30.182331700736384 32.5 29.0 34.5 22.5 36.0 78-79 30.982632510298302 34.0 30.5 35.0 24.5 36.0 80-81 31.336290574068954 34.0 31.0 35.0 26.0 36.0 82-83 31.44899433259338 34.0 31.0 35.0 25.5 36.0 84-85 31.295455450893034 34.0 31.5 35.0 25.0 35.5 86-87 30.469172476818034 34.0 31.0 35.0 21.5 35.0 88-89 29.45580044085765 33.5 29.0 35.0 18.5 35.0 90-91 28.821391365191843 33.0 28.5 34.0 13.5 35.0 92-93 29.086684196973405 33.0 29.0 35.0 13.0 35.0 94-95 29.24059865178336 34.0 29.0 35.0 4.5 35.0 96-97 28.218763437175497 33.0 29.0 35.0 2.0 35.0 98-99 27.57229305356889 33.0 28.0 35.0 2.0 35.0 100-101 24.53535294637524 30.0 21.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07084250115361357 1101 2 0.11133748061887871 1101 3 0.10863644605213096 1101 4 0.07730629211815199 1101 5 0.022563691463979296 1101 6 0.10421898405378727 1101 7 0.040077014271339806 1101 8 0.06558136421415384 1101 9 0.13876891730551932 1101 10-11 0.1536075921699478 1101 12-13 0.15433949838533323 1101 14-15 0.26653328035812507 1101 16-17 0.2226997521886389 1101 18-19 0.17894842331498495 1101 20-21 0.18729872677228343 1101 22-23 0.17239988108892845 1101 24-25 0.20739382046772192 1101 26-27 0.28482901281898876 1101 28-29 0.34464332415834065 1101 30-31 0.2731424111510137 1101 32-33 0.3181940172333029 1101 34-35 0.19320033736966735 1101 36-37 0.21400803935193125 1101 38-39 0.28598475521857836 1101 40-41 0.3303663580662288 1101 42-43 0.26763723423022157 1101 44-45 0.3577402662408531 1101 46-47 0.398884783412214 1101 48-49 0.35052644687748824 1101 50-51 0.40295780804527226 1101 52-53 0.4852672073495441 1101 54-55 0.3508632120195827 1101 56-57 0.21365111426928962 1101 58-59 0.4599536652743339 1101 60-61 0.48915887672647074 1101 62-63 0.3026359324925636 1101 64-65 0.39356598277656474 1101 66-67 0.2557886953909332 1101 68-69 0.3071527359550643 1101 70-71 0.2845500408055841 1101 72-73 0.34486264494429264 1101 74-75 0.34880145744473623 1101 76-77 0.3807482687575856 1101 78-79 0.44768849651240217 1101 80-81 0.4456427130398559 1101 82-83 0.421531190076454 1101 84-85 0.39989252174183676 1101 86-87 0.501666514992877 1101 88-89 0.6227585625462417 1101 90-91 0.7027657716340734 1101 92-93 0.6492572540918395 1101 94-95 0.7796986929895233 1101 96-97 0.7477880875266187 1101 98-99 0.8604419345405283 1101 100-101 1.42017582936095 1102 1 0.002982352210157302 1102 2 0.043241256068810685 1102 3 0.09759026587830277 1102 4 0.0914216211945984 1102 5 0.032090952551982355 1102 6 0.06594734304277239 1102 7 0.056652244528059725 1102 8 0.04936077862047483 1102 9 0.10096594115911728 1102 10-11 0.1695099239395148 1102 12-13 0.15790488750998577 1102 14-15 0.15185694798359606 1102 16-17 0.2980160553176958 1102 18-19 0.21624521484495318 1102 20-21 0.1872023375337477 1102 22-23 0.10713115167705922 1102 24-25 0.06168769043754452 1102 26-27 0.20149236468752463 1102 28-29 0.2456557763995164 1102 30-31 0.18225356823027994 1102 32-33 0.20919726219378987 1102 34-35 0.16639073151631578 1102 36-37 0.13821540139131372 1102 38-39 0.19699051035006931 1102 40-41 0.25971211652469606 1102 42-43 0.2619624381733132 1102 44-45 0.11172904750302592 1102 46-47 -0.03121952149785301 1102 48-49 0.08252993189797309 1102 50-51 0.16151166399665584 1102 52-53 0.2464854969336301 1102 54-55 0.19911709921529308 1102 56-57 0.06090279244809693 1102 58-59 0.11168213051394105 1102 60-61 0.02977037231358537 1102 62-63 -0.10672727099033352 1102 64-65 0.005662682798188712 1102 66-67 0.1658429429754804 1102 68-69 0.15237748424899422 1102 70-71 -0.0030313031784885425 1102 72-73 0.0694052609077147 1102 74-75 0.11685104793158274 1102 76-77 0.09491640771480903 1102 78-79 0.16689221780771746 1102 80-81 0.09487868804041355 1102 82-83 0.16699388996459064 1102 84-85 0.26303029526663835 1102 86-87 0.1426314101185291 1102 88-89 0.25890844498988486 1102 90-91 0.24536785607211087 1102 92-93 0.44122975377391427 1102 94-95 0.4324183935486978 1102 96-97 0.4342979769695212 1102 98-99 0.5577885908167737 1102 100-101 1.0367811086224528 1103 1 0.011582140623399795 1103 2 0.03246260098567433 1103 3 0.03107846985462004 1103 4 0.06942728660357744 1103 5 0.0013796906170426837 1103 6 0.03680981993768029 1103 7 0.04955487641978351 1103 8 0.1045949756689879 1103 9 0.04050844012530064 1103 10-11 0.006723438044247132 1103 12-13 0.0652306717700526 1103 14-15 0.13280169235287786 1103 16-17 0.00911690437965973 1103 18-19 0.13573711068218586 1103 20-21 0.06303124471007493 1103 22-23 -0.001045904594626279 1103 24-25 0.054850082432921 1103 26-27 0.15323231600739007 1103 28-29 0.21816597794929038 1103 30-31 0.11371070254207893 1103 32-33 0.13425373354179015 1103 34-35 0.10056495555981115 1103 36-37 -0.032803690476193026 1103 38-39 0.2430308731422599 1103 40-41 0.13937020294198277 1103 42-43 0.21132222104746745 1103 44-45 0.23244768547542805 1103 46-47 0.25796868221490143 1103 48-49 0.259951734834722 1103 50-51 0.3509290618237344 1103 52-53 0.44012100764015827 1103 54-55 0.18129105614773522 1103 56-57 0.17330661444785278 1103 58-59 0.03693294960529414 1103 60-61 0.14849790249305528 1103 62-63 0.08403695463417193 1103 64-65 0.13795229100640682 1103 66-67 -0.003343856594284489 1103 68-69 0.020526218238806848 1103 70-71 0.016640670346390607 1103 72-73 0.12958637884242563 1103 74-75 0.23341239699356464 1103 76-77 0.22320058847532565 1103 78-79 0.21702377444820087 1103 80-81 0.24154833044024926 1103 82-83 0.24299216117306344 1103 84-85 0.20948216531234465 1103 86-87 0.22180259359864607 1103 88-89 0.4411648881788217 1103 90-91 0.3612188618300145 1103 92-93 0.2992833124619345 1103 94-95 0.2783448340471537 1103 96-97 0.30550896202917954 1103 98-99 0.4397955366568276 1103 100-101 0.9151597483241538 1104 1 -0.005368941800998783 1104 2 0.020174153752343216 1104 3 -0.025086580994219787 1104 4 -0.0033889028893412387 1104 5 -0.005980522120367482 1104 6 0.018889547975675214 1104 7 -0.08822347772055394 1104 8 0.013972346833327265 1104 9 -0.048065536186499 1104 10-11 0.082926071909597 1104 12-13 0.031114774162581682 1104 14-15 0.045810710409043054 1104 16-17 0.11398613269397373 1104 18-19 0.10362359638053675 1104 20-21 -0.019347219423835327 1104 22-23 0.021839547271135018 1104 24-25 0.04857844355014862 1104 26-27 0.11292904364204048 1104 28-29 0.15327659377798142 1104 30-31 0.09335078643265149 1104 32-33 0.047455828998934635 1104 34-35 0.049121098905807514 1104 36-37 0.00855705102620874 1104 38-39 0.12187603604839126 1104 40-41 0.1407444382640648 1104 42-43 0.13876104460833005 1104 44-45 0.15095714314999498 1104 46-47 0.1656557363280129 1104 48-49 0.09609182403362837 1104 50-51 0.1614014316019734 1104 52-53 0.15193616427393408 1104 54-55 0.07407373346355683 1104 56-57 0.04824078119901998 1104 58-59 0.08390551451429928 1104 60-61 0.1630282925930615 1104 62-63 -0.05915200809179311 1104 64-65 0.09724073309871528 1104 66-67 0.1277869706996384 1104 68-69 0.23718148187037258 1104 70-71 0.10453936247895967 1104 72-73 0.013254050624958325 1104 74-75 0.04123691114319428 1104 76-77 0.06718677804155959 1104 78-79 0.13051291549469468 1104 80-81 0.11261570167667756 1104 82-83 0.0718142913780504 1104 84-85 0.1939431001750549 1104 86-87 0.23161134841002706 1104 88-89 0.3976989124658097 1104 90-91 0.3354270733720277 1104 92-93 0.37143337427438183 1104 94-95 0.4143301571226523 1104 96-97 0.6106349860246212 1104 98-99 0.5971779546642608 1104 100-101 0.9886751475321383 1105 1 -0.031123554971408396 1105 2 -0.04961025925677376 1105 3 -0.021408924473831803 1105 4 -0.06159473291965867 1105 5 -0.06883271250173806 1105 6 -0.03500131505560944 1105 7 -0.03281829744098275 1105 8 -0.03539326189910241 1105 9 0.0058500801352963094 1105 10-11 -0.013187904385254967 1105 12-13 -0.030394159823373457 1105 14-15 -0.011789690571127664 1105 16-17 -0.08952508397334924 1105 18-19 -0.1246980226604748 1105 20-21 -0.10633259569452491 1105 22-23 -0.10817481223993042 1105 24-25 -0.06953442688566724 1105 26-27 -0.05506344223058335 1105 28-29 -0.05618846881763062 1105 30-31 -0.12857158649643452 1105 32-33 -0.014010654250476762 1105 34-35 0.028312083294714796 1105 36-37 -0.020280315546401084 1105 38-39 -0.00841114463018755 1105 40-41 -0.028665737379910183 1105 42-43 -0.08921875635959253 1105 44-45 -0.048603205444578634 1105 46-47 -0.003072870763553226 1105 48-49 0.04114987151199756 1105 50-51 0.10836866336371287 1105 52-53 0.10166781228988953 1105 54-55 -0.004068732191463198 1105 56-57 0.0021908554892533516 1105 58-59 0.038385861543865474 1105 60-61 -0.03803823300298603 1105 62-63 -0.2214272501658172 1105 64-65 -0.13555517333165312 1105 66-67 -0.05231918563745808 1105 68-69 -0.009130241695750385 1105 70-71 -0.10559447272155609 1105 72-73 -0.21997519743514005 1105 74-75 -0.17192615136050904 1105 76-77 -0.1369378344183474 1105 78-79 -0.15742829058219954 1105 80-81 -0.10208035871279364 1105 82-83 -0.059539218258283455 1105 84-85 -0.003602689147086835 1105 86-87 0.07052889106412152 1105 88-89 0.21249138056002081 1105 90-91 0.16917477852367213 1105 92-93 0.1752864928752338 1105 94-95 0.2190030115914361 1105 96-97 0.12987513172086906 1105 98-99 0.3035775420014595 1105 100-101 0.6332156546004022 1106 1 -0.04442850531131981 1106 2 -0.1404075640997 1106 3 -0.06212305417168551 1106 4 -0.04654513927225423 1106 5 -0.06416203153967359 1106 6 -0.08908464855042553 1106 7 -0.038697115033414775 1106 8 -0.03936097515981629 1106 9 -0.05892618024706309 1106 10-11 -0.04899771802808317 1106 12-13 -0.024140313543881575 1106 14-15 0.0509889683116711 1106 16-17 -0.04136003093309881 1106 18-19 -0.1385259285929763 1106 20-21 -0.12029490513153718 1106 22-23 -0.16064786440647794 1106 24-25 -0.14996289584565403 1106 26-27 -0.18761264900075503 1106 28-29 -0.004791641294005444 1106 30-31 -0.04104724921921843 1106 32-33 -0.06963330236217047 1106 34-35 -0.010910818065845262 1106 36-37 -0.09920335452175522 1106 38-39 -0.12081377821910166 1106 40-41 -0.07588997973033429 1106 42-43 -0.015153192918234026 1106 44-45 0.04839458568711308 1106 46-47 0.002345639049003978 1106 48-49 0.024728531612872473 1106 50-51 0.04758655400659961 1106 52-53 0.03787160399131295 1106 54-55 0.05207245599087429 1106 56-57 -0.06714715119188952 1106 58-59 -0.024939920647156555 1106 60-61 -0.09030148558994 1106 62-63 -0.12277143747031971 1106 64-65 -0.18411538098021651 1106 66-67 -0.1635724547154922 1106 68-69 -0.12883696522068533 1106 70-71 -0.11084563563714767 1106 72-73 -0.04616562353162834 1106 74-75 0.06888736218517266 1106 76-77 -0.024988560045013486 1106 78-79 -0.02509590116472893 1106 80-81 -0.1765917237679382 1106 82-83 0.08835189247941244 1106 84-85 0.010174445204093274 1106 86-87 0.11837606805070067 1106 88-89 0.19950870795394593 1106 90-91 0.099936183373881 1106 92-93 0.18903227563653857 1106 94-95 0.374828524560197 1106 96-97 0.3188469296854102 1106 98-99 0.37527108429371836 1106 100-101 1.018781742506107 1107 1 -0.09328704898129203 1107 2 -0.08069575269585272 1107 3 -0.12674068571924835 1107 4 -0.0420664651072542 1107 5 -0.04482945667590599 1107 6 -0.11806588314264133 1107 7 -0.0680318778914355 1107 8 -0.08198136552853441 1107 9 -0.11592106097241128 1107 10-11 -0.09929900826011817 1107 12-13 -0.11892825319316103 1107 14-15 -0.13106875471564194 1107 16-17 -0.10832657041876104 1107 18-19 -0.060617495757682605 1107 20-21 -0.058443875154964076 1107 22-23 -0.2235931021487758 1107 24-25 -0.17711940641871848 1107 26-27 -0.13914815493427568 1107 28-29 -0.09378938721231833 1107 30-31 -0.1672845915671246 1107 32-33 -0.10407733460125002 1107 34-35 -0.009234303669707344 1107 36-37 -0.08507931183506656 1107 38-39 -0.028870784026636898 1107 40-41 -0.1157503904360766 1107 42-43 -0.13483844033404324 1107 44-45 -0.1928193140532457 1107 46-47 -0.2632494414131017 1107 48-49 -0.45609197413239144 1107 50-51 -0.3007286509210516 1107 52-53 -0.2557461405104675 1107 54-55 -0.360275809503662 1107 56-57 -0.31936604329796836 1107 58-59 -0.298801630458037 1107 60-61 -0.43870452624371126 1107 62-63 -0.49479800988384426 1107 64-65 -0.5138443645844859 1107 66-67 -0.3824530284070562 1107 68-69 -0.2649844075975807 1107 70-71 -0.4371829121872537 1107 72-73 -0.37251443247023275 1107 74-75 -0.35720927449113304 1107 76-77 -0.38506957902369265 1107 78-79 -0.324575100606328 1107 80-81 -0.3779339568754203 1107 82-83 -0.263126451775026 1107 84-85 -0.37386708561252746 1107 86-87 -0.3900231179417126 1107 88-89 -0.19452674334554132 1107 90-91 -0.21294602117646022 1107 92-93 -0.14556868134107148 1107 94-95 -0.03594583320029798 1107 96-97 -0.0584497468013403 1107 98-99 -0.09517045610689578 1107 100-101 0.33512553301263637 1108 1 -0.1826770149146988 1108 2 -0.12072978747123742 1108 3 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-1.1958517439219278 2316 60-61 -1.11766464568192 2316 62-63 -1.0893885142171555 2316 64-65 -1.268739756216938 2316 66-67 -1.2316984320880806 2316 68-69 -1.2623697619527405 2316 70-71 -1.2056937832087016 2316 72-73 -1.3853399174803762 2316 74-75 -1.3915171684087646 2316 76-77 -1.411844133130991 2316 78-79 -1.3693043174706538 2316 80-81 -1.3336746979083713 2316 82-83 -1.6240129406188508 2316 84-85 -1.715018376514628 2316 86-87 -2.0313489702696614 2316 88-89 -2.4602184680966594 2316 90-91 -2.9155517496810823 2316 92-93 -3.1131290006193986 2316 94-95 -3.0365713479505274 2316 96-97 -3.4264629598729393 2316 98-99 -3.8802669731136312 2316 100-101 -4.472418593024596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 11.0 5 27.0 6 122.0 7 351.0 8 915.0 9 1692.0 10 2798.0 11 4232.0 12 6757.0 13 9829.0 14 11473.0 15 13418.0 16 16191.0 17 20163.0 18 22560.0 19 23885.0 20 27168.0 21 30849.0 22 34942.0 23 40689.0 24 48267.0 25 60575.0 26 74068.0 27 93601.0 28 116647.0 29 145090.0 30 178932.0 31 220550.0 32 273775.0 33 346480.0 34 455218.0 35 629348.0 36 1013858.0 37 1740766.0 38 876239.0 39 9828.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 70.77099420387249 9.289125929140123 6.640594894829604 13.299284972157782 2 7.846264898331191 26.40665905395263 34.14784590771846 31.59923013999772 3 32.471909100469965 24.905541951004995 36.287231231685944 6.335317716839092 4 6.380896310835632 11.007322034746887 61.8090458397627 20.802735814654785 5 9.069758602883295 59.412628384412855 23.224888537746814 8.292724474957035 6 13.253388133477204 34.018330043666 36.730653252985164 15.997628569871633 7 21.961891349851427 56.181615890902 9.446476519773645 12.410016239472926 8 11.091976717367382 27.81945809097127 16.314190680017056 44.7743745116443 9 26.146635159535002 27.136847667821495 29.58076699340484 17.13575017923866 10-11 17.472789075668864 19.124639137663994 30.51773069889264 32.88484108777451 12-13 46.882750822315074 11.461032057504127 20.088292116963892 21.56792500321691 14-15 41.09970905798852 16.606018449299913 32.98201404284993 9.312258449861636 16-17 20.684515802521545 23.074846529685352 40.83158689370038 15.40905077409272 18-19 9.835259703343368 40.41573202556465 33.181081375843604 16.567926895248377 20-21 16.050046704324725 40.71568472340805 12.394318061526679 30.839950510740543 22-23 18.145493099788577 30.131984068556555 10.80401952522247 40.9185033064324 24-25 19.687800368811114 27.48231234586124 23.86755281873686 28.962334466590782 26-27 37.70501524771033 18.461219558862375 21.540351119008765 22.293414074418536 28-29 19.58637044345713 18.318305575318323 48.560244567469674 13.535079413754875 30-31 12.990822766889304 43.61839390864834 31.64354944598196 11.747233878480385 32-33 15.920438030742195 40.89888894163482 14.355944396557579 28.824728631065398 34-35 23.609994629694704 18.95391093475369 22.735480559891727 34.700613875659876 36-37 36.981544447945595 20.405103703323736 29.621712173507348 12.991639675223322 38-39 40.86044601092163 9.986994280429693 30.229821651656923 18.92273805699175 40-41 20.132620159876314 18.168902368469016 50.11462697612846 11.583850495526205 42-43 18.264020387462534 38.09525445021703 27.559152415210608 16.081572747109828 44-45 11.849878846660506 42.09401572413734 21.005839844352227 25.050265584849924 46-47 15.665437174067943 24.150981528303465 10.717581723809273 49.46599957381932 48-49 24.100276600788327 26.9752396425819 15.115582068282738 33.808901688347035 50-51 39.63178532706627 26.838757025909565 22.16912493682539 11.360332710198774 52-53 19.891018925801376 24.940618846183845 35.70417324994455 19.464188978070222 54-55 10.53073876245722 32.63161734862328 41.5905891346188 15.247054754300699 56-57 16.447514936300635 42.58960139489425 25.58364946550474 15.379234203300374 58-59 18.024347556375886 26.166754232064687 24.162379218517746 31.646518993041678 60-61 17.92248995616124 18.403128854208642 30.163143691003896 33.51123749862622 62-63 43.363361373988546 12.075411966533162 22.151769085959916 22.40945757351838 64-65 37.37801124383922 16.521728976491918 34.071304092716574 12.028955686952296 66-67 19.71605096190862 22.880426105201973 40.031102480352594 17.372420452536815 68-69 10.159257646340688 38.52345598281616 33.29029588304315 18.026990487800003 70-71 15.218835282634336 38.75580247235828 14.439320951316589 31.58604129369079 72-73 17.36480322060238 29.716571665470216 12.278447354357999 40.640177759569404 74-75 19.222245406689733 26.779507522223376 24.339938464318873 29.658308606768006 76-77 33.475793922903826 18.886570568412253 24.08687101296752 23.550764495716404 78-79 17.993644736316718 18.694615616181224 46.063354593128985 17.248385054373074 80-81 13.53939565452392 39.888303408824996 30.65691809542475 15.915382841226325 82-83 16.6547708469788 37.778861698274284 16.124427424636956 29.441940030109958 84-85 22.185311939378334 18.31845821628951 23.372263395428188 36.12396644890397 86-87 32.47214407554422 19.246049716956033 29.27493921068709 19.006866996812654 88-89 35.70909892205945 12.31099901184061 30.194210914222776 21.785691151877174 90-91 19.280344649429043 18.865657788671943 45.2941122899407 16.55988527195831 92-93 17.490793078220406 34.14884570607973 28.463747998304463 19.89661321739539 94-95 12.724887919516664 37.55078175860464 23.016937505256575 26.707392816622118 96-97 14.901990354767463 24.80614019816924 14.784044649325986 45.507824797737314 98-99 21.3632207977994 26.534050411455972 17.03032302630841 35.07240576443622 100-101 32.43022613474545 25.249978210335062 24.104421874778193 18.215373780141288 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 109.0 1 83.0 2 66.5 3 94.0 4 98.5 5 102.5 6 150.5 7 250.0 8 354.5 9 424.5 10 461.0 11 490.0 12 552.5 13 655.5 14 786.5 15 888.5 16 978.0 17 1082.0 18 1211.5 19 1367.5 20 1546.0 21 1696.0 22 1968.5 23 2289.0 24 2559.5 25 2988.0 26 3676.0 27 4358.5 28 4836.5 29 5394.0 30 6128.0 31 6873.0 32 7568.0 33 8389.5 34 9456.0 35 10258.5 36 11065.5 37 12048.0 38 12525.0 39 12620.0 40 13882.0 41 21029.5 42 58431.5 43 182189.0 44 575362.5 45 1129373.5 46 1408062.5 47 1229027.5 48 800762.5 49 446182.0 50 247897.5 51 129740.5 52 54591.0 53 23496.0 54 15092.0 55 12482.0 56 10779.5 57 9395.0 58 8499.0 59 7615.5 60 6516.0 61 5350.5 62 4196.0 63 3264.5 64 2490.0 65 1901.5 66 1362.0 67 1048.0 68 786.0 69 495.0 70 335.0 71 270.5 72 243.0 73 214.0 74 176.5 75 142.0 76 92.5 77 52.0 78 36.0 79 21.5 80 10.5 81 8.5 82 7.5 83 5.0 84 1.5 85 2.0 86 3.0 87 1.5 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3773590089693361 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3659350534037334E-4 16-17 0.002068285159588425 18-19 1.4500892262797076E-4 20-21 7.632048559366883E-6 22-23 0.0 24-25 0.0 26-27 1.4500892262797076E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007372558908348407 36-37 0.016744714539250936 38-39 0.011318328013541086 40-41 0.007738897239198018 42-43 0.003602326920021168 44-45 0.001816427557129318 46-47 0.00166378658594198 48-49 3.052819423746753E-5 50-51 5.18979302036948E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.1213062220581164E-4 60-61 6.258279818680844E-4 62-63 0.0 64-65 0.0 66-67 0.0011448072839050322 68-69 7.632048559366882E-4 70-71 0.0019309082855198212 72-73 0.0 74-75 0.0 76-77 0.0969728089953156 78-79 0.03451975563401641 80-81 3.052819423746753E-5 82-83 0.0 84-85 0.0 86-87 0.14759618708959613 88-89 0.32051551129917155 90-91 0.2741050240096615 92-93 0.0 94-95 3.052819423746753E-5 96-97 2.2896145678100644E-5 98-99 1.9080121398417206E-4 100-101 7.632048559366882E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6551321.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.082840324528002 #Duplication Level Percentage of deduplicated Percentage of total 1 75.93621593014268 8.415889560026509 2 11.056038733088327 2.4506462380122955 3 3.724588171198058 1.2383704792804153 4 1.956041077746126 0.867139637315119 5 1.2721068247563154 0.7049278407258285 6 0.8521456041215643 0.5666516198236647 7 0.6406725537442365 0.497033012941847 8 0.4943183652147674 0.43827612089255874 9 0.40555185753748324 0.4045199832363289 >10 2.7271075551133004 6.063355722296522 >50 0.43523698474340883 3.394924805688725 >100 0.36626557849511276 8.521090652961057 >500 0.07057722806025032 5.5097621754556325 >1k 0.0485218442914221 9.592383033176493 >5k 0.00578953823931741 4.53701265584308 >10k+ 0.00882215350753129 46.79801646232393 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 381613 5.824977893771348 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 230596 3.5198397391915313 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 229992 3.5106202245318157 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 211329 3.2257463800048876 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 181939 2.7771345656853024 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 149497 2.2819367269593416 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 120412 1.83798046226097 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 112846 1.7224923034606303 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 111365 1.6998861756277857 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 110859 1.6921625424857063 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 97752 1.4920960215504628 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 91440 1.3957490405370154 No Hit CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 67691 1.0332419980642071 No Hit CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA 38724 0.5910868968258463 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 38603 0.5892399410744794 No Hit GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT 37581 0.5736400338191336 No Hit CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 37057 0.565641646928917 No Hit GCTTTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 35024 0.5346097374865313 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 32244 0.4921755474964515 No Hit GAGTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 30492 0.46543284934442997 No Hit GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG 29694 0.45325209984368037 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA 29390 0.44861181431958536 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 25967 0.3963628098821596 No Hit TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC 24625 0.37587839154881897 No Hit ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA 22966 0.3505552544288396 No Hit TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG 22911 0.34971572908730925 No Hit GCTTTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 22273 0.3399772351255571 No Hit CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 21375 0.3262700759129342 No Hit GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT 20924 0.3193859681123853 No Hit GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT 20795 0.3174168995840686 No Hit GCTTATACTCTGCGTTGATACCACTGCTTTTACTCTGCGTTGATACCACT 20422 0.3117233913587809 No Hit AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG 20175 0.3079531593704537 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT 19796 0.3021680665624536 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 19179 0.29275011864019485 No Hit GAGTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18173 0.27739443693874866 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC 17439 0.2661905896535981 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 17179 0.2622219244027273 No Hit GTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 16668 0.2544219707750544 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG 16553 0.2526665996064 No Hit GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCA 16171 0.2468357145070437 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG 16116 0.24599618916551336 No Hit GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 15960 0.2436149900149909 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 15142 0.23112895857186666 No Hit ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTAT 14613 0.22305425119605649 No Hit TGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13727 0.20953026114885837 No Hit CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAG 13517 0.2063248007539243 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 12991 0.19829588566947035 No Hit CCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATA 12402 0.18930533246653614 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG 12289 0.18758048949211922 No Hit TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 12153 0.18550457228397144 No Hit ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 11971 0.18272650660836187 No Hit GTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCAGA 11805 0.18019266648665208 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 11754 0.17941419753359666 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA 11613 0.1772619598398552 No Hit AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAT 11449 0.17475864791238288 No Hit ATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG 11208 0.17108000050676803 No Hit AGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 11145 0.1701183623882878 No Hit GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 10815 0.16508121033910567 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA 10809 0.16498962575639325 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 10790 0.1646996079111373 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGT 10671 0.162883180354008 No Hit GATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTT 10303 0.15726599261431398 No Hit GTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGC 10159 0.1550679626292163 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 10112 0.15435055006463583 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA 10000 0.15264097118733763 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG 9924 0.15148089980631388 No Hit GTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9912 0.15129773064088906 No Hit GCTTCTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 9534 0.1455279019300077 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 9497 0.14496313033661457 No Hit TGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGT 8772 0.13389665992553257 No Hit GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCA 8682 0.13252289118484653 No Hit GCTTACACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 8675 0.1324160425050154 No Hit GTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGCTT 8290 0.12653936511430292 No Hit ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGT 8169 0.12469240936293612 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCA 8137 0.12420395825513664 No Hit ATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGT 8126 0.12403605318683057 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTAT 8111 0.12380709173004956 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 7958 0.12147168487088328 No Hit CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAG 7815 0.11928891898290436 No Hit TACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGA 7766 0.1185409782240864 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 7697 0.11748775552289378 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAG 7664 0.11698404031797557 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7663 0.11696877622085684 No Hit AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAC 6867 0.10481855491434476 No Hit CCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 6850 0.10455906526332628 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.5264097118733765E-5 0.0 9 0.0 0.0 0.0 1.3737687406860388E-4 0.0 10-11 0.0 0.0 0.0 2.7475374813720776E-4 0.0 12-13 0.0 0.0 0.0 4.4265881644327916E-4 0.0 14-15 0.0 0.0 0.0 5.418754477150487E-4 0.0 16-17 0.0 0.0 0.0 8.853176328865583E-4 0.0 18-19 0.0 0.0 0.0 0.0011982316238206006 0.0 20-21 0.0 0.0 0.0 0.0017630032172137496 0.0 22-23 0.0 0.0 0.0 0.0032817808805277594 0.0 24-25 0.0 0.0 0.0 0.0051974250689288464 0.0 26-27 0.0 0.0 0.0 0.00719702179148297 0.0 28-29 0.0 0.0 0.0 0.012890530016770664 0.0 30-31 0.0 0.0 0.0 0.03397788018630136 0.0 32-33 0.0 0.0 0.0 0.08371594064769533 0.0 34-35 0.0 0.0 0.0 0.36255283476416433 0.0 36-37 0.0 0.0 0.0 0.7986633535435068 0.0 38-39 0.0 0.0 0.0 0.9493963125910027 0.0 40-41 0.0 0.0 0.0 1.0842622426835749 0.0 42-43 0.0 0.0 0.0 1.2534800233418575 0.0 44-45 0.0 0.0 0.0 1.4432661748676336 0.0 46-47 1.5264097118733765E-5 0.0 0.0 1.7506240344504564 0.0 48-49 1.5264097118733765E-5 0.0 0.0 2.1158022939190433 0.0 50-51 1.5264097118733765E-5 7.632048559366883E-6 0.0 2.414062751619101 0.0 52-53 1.5264097118733765E-5 1.5264097118733765E-5 0.0 2.6193419617203917 0.0 54-55 1.5264097118733765E-5 1.5264097118733765E-5 0.0 2.8328256240230023 0.0 56-57 1.5264097118733765E-5 1.5264097118733765E-5 0.0 3.043470164261528 0.0 58-59 1.5264097118733765E-5 1.5264097118733765E-5 0.0 3.9203391193928674 0.0 60-61 1.5264097118733765E-5 1.5264097118733765E-5 0.0 5.7960219015371095 0.0 62-63 1.5264097118733765E-5 1.5264097118733765E-5 0.0 6.9251300615555245 0.0 64-65 1.5264097118733765E-5 1.5264097118733765E-5 0.0 7.271579273859425 0.0 66-67 1.5264097118733765E-5 1.5264097118733765E-5 0.0 7.700996486052202 0.0 68-69 1.5264097118733765E-5 1.5264097118733765E-5 0.0 8.078034948982044 0.0 70-71 1.5264097118733765E-5 1.5264097118733765E-5 0.0 8.59236633344634 0.0 72-73 1.5264097118733765E-5 1.5264097118733765E-5 0.0 9.380756949628937 0.0 74-75 1.5264097118733765E-5 1.5264097118733765E-5 0.0 10.059703989470215 0.0 76-77 1.5264097118733765E-5 1.5264097118733765E-5 0.0 10.543614028376872 0.0 78-79 1.5264097118733765E-5 1.5264097118733765E-5 0.0 10.979793235593249 0.0 80-81 1.5264097118733765E-5 1.5264097118733765E-5 0.0 11.474655264182598 0.0 82-83 1.5264097118733765E-5 1.5264097118733765E-5 0.0 12.064147062859536 0.0 84-85 1.5264097118733765E-5 1.5264097118733765E-5 0.0 14.803251130573514 0.0 86-87 1.5264097118733765E-5 1.5264097118733765E-5 0.0 18.178814623798772 0.0 88-89 1.5264097118733765E-5 1.5264097118733765E-5 0.0 18.979439413822035 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCACTC 5965 0.0 38.065926 94-95 TATGTAC 740 0.0 35.268955 4 TTATGTA 690 0.0 34.386066 3 CTTATGT 750 0.0 32.267887 2 GCGCTTA 120 1.4712896E-7 31.754906 1 TACGCTT 110 0.0 30.197449 94-95 GCGTTAG 65 0.005767097 29.312222 1 GCGTCAG 100 4.863483E-5 28.579416 1 CCAAGAC 245 0.0 27.11587 3 TATAGGG 90 9.0041955E-4 26.36265 2 CAACGGC 505 0.0 26.310448 18-19 GCTTACA 8725 0.0 25.65869 1 ACACTCT 8070 0.0 25.637434 5 TACACTC 8215 0.0 25.358208 4 GCTTCCC 2580 0.0 25.10853 1 CTTACAC 8595 0.0 24.789175 2 TACCTGG 690 0.0 24.757967 2 GTACCCG 135 1.3153131E-5 24.698261 1 TTACACT 8505 0.0 24.660934 3 TAAACTC 1330 0.0 24.618355 4 >>END_MODULE