FastQCFastQC Report
Thu 26 May 2016
SRR938860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6528572
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT3677515.632946990551686No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA2355383.6078027476759083No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2203533.37520977022234No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA2119453.2464220353241107No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA1732162.653198892499003No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1429552.189682521690808No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1236431.8938751077571023No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1212951.857910121845941No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1135491.7392624298238575No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1114721.7074484282320852No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA988341.5138685764666455No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT977371.4970655144800424No Hit
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG621650.952199041383016No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA374650.5738620941915016No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT362630.555450717247202No Hit
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG348900.5344200845146535No Hit
CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG343660.5263938270114813No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT332580.509422274886453No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG308460.47247698271536254No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG299310.45846166665543403No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA294410.4509561968528493No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA285970.4380284080500299No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA253470.3882472307879885No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC226540.34699778144439547No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA216610.33178771713017796No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG212270.3251400153050315No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA208110.3187680246154902No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT204250.3128555524852908No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT203280.3113697758100853No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT201210.30819909775062604No Hit
GCTTATACTCTGCGTTGATACCACTGCTTTTACTCTGCGTTGATACCACT195750.299835859970603No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT192710.2951794052359383No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT188480.2887001935492172No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG184080.28196058801220236No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA176980.27108531544111025No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC167040.2558599338415813No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG166640.2552472424291254No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG163510.25045293212665803No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG159840.2448314884173752No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC157960.24195183877883247No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCA155980.2389190162871758No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA146870.22496496936849283No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG142790.21871551696144273No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTAT134530.2060634392942285No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAG132260.2025864155285413No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATA124370.1905010774178488No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC123520.18919910816638003No Hit
GTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCAGA121170.18559954611820165No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG116960.17915096900210337No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA114460.17532164767425404No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG112180.17182930662325543No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG111960.17149232634640468No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA111050.17009845338306753No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA110730.16960830025310283No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG110140.16870458041973038No Hit
AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAT108590.16633040119646378No Hit
ATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG106590.16326694413418433No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGT106310.1628380601454652No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGT105730.16194965759740415No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA103590.15867175854076512No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG100060.15326475682584184No Hit
GTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGC99500.1524069888484036No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT98780.151304144305983No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA96060.1471378427012829No Hit
GCTTCTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT92820.14217504226039018No Hit
GATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTT92190.14121005328577213No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG91660.14039823716426808No Hit
GTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGCTT88780.13598685899458565No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG88520.13558860957648933No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA87890.13462362060187127No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCA82760.12676585323712444No Hit
GCTTACACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT81840.1253566629884759No Hit
TGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGT79560.12186432193747729No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCA78340.11999561312948681No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGT76180.11668707950222498No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTAT75960.11635009922537426No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA75600.11579867695416393No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAG73540.11264331618001609No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAG73320.11230633590316534No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG73050.11189276919975763No Hit
TACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGA73040.11187745191444622No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT70720.10832384172220204No Hit
ATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGT70670.10824725529564505No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA69260.10608751806673804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC7150.034.5546594-95
ACCACTC38800.033.30783594-95
GCCGGAT3100.032.18598694-95
GACGATA452.4108545E-531.67436888-89
CGCCGTT1150.030.9865194-95
TTATGTA5850.030.0064243
TATGTAC6100.029.55444
CGTATCT1550.029.12065394-95
TACCTGG6050.028.23032
GCTTCCC23600.027.991681
CTTAGGG42250.027.38302894-95
GCCGTAT12350.027.31496494-95
GCTTACA85300.026.2977851
ACACTCT78650.026.1190625
TACACTC81300.025.9096074
CTTCCCA25000.025.6189982
CTTACAC84450.025.111712
TTACACT85300.024.6946223
CAAGGTT1351.3566329E-524.5998614
TTCCCAT24550.024.3493543
Produced by FastQC (version 0.11.5)