FastQCFastQC Report
Thu 26 May 2016
SRR938860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6528572
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT3836985.877211739412539No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA2311133.540023760172975No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2303053.5276473936413657No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA2116743.242271051004722No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA1822522.791605882572789No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1494352.2889385305086623No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1198911.8364046532687393No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1139291.7450829982421885No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1117971.7124265459582892No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1114491.7070961306699228No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA978121.4982143108783974No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT907951.390732919848322No Hit
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG675771.0350961894882986No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA383860.5879693139632985No Hit
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG381750.5847373667625938No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT373980.572835836075638No Hit
CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG373980.572835836075638No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT355350.5442997335405048No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG325310.4982866084650671No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA305040.4672384711388647No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG298210.4567767652711803No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA289700.4437417554711811No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA263250.4032275358225351No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC248280.3802975597113733No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA232290.355805220498449No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG227230.3480546741308819No Hit
GCTTTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT225980.3461400134669572No Hit
CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA214560.32864767364134145No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT210060.32175489525121265No Hit
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT205820.3152603662791802No Hit
GCTTATACTCTGCGTTGATACCACTGCTTTTACTCTGCGTTGATACCACT205000.3140043488836456No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG204240.3128402351999794No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT195470.299406975981884No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT190910.2924222938798867No Hit
GAGTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA181590.2781465839696644No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC178180.27292338967847796No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC173110.2651575260255995No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG165980.25423630159857313No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA165820.2539912250335908No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG161690.24766518619998368No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCA161280.2470371775022164No Hit
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG156860.2402669373945788No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG151850.23259297745356874No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTAT145070.2222078580124413No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC136990.20983149148083224No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAG135040.20684462084510977No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG127710.1956170507118555No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATA123740.18953608844323078No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG122240.18723849564652117No Hit
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG119960.18374615459552257No Hit
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA119060.18236759891749682No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG117670.18023849625921257No Hit
AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAT115990.17766519232689781No Hit
GTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCAGA115540.17697591448788494No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA113580.17397372656685106No Hit
AGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG110740.1696236175384142No Hit
ATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG110420.1691334644084495No Hit
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGT109280.1673872938829502No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA109100.16711158274734506No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA108420.16607000734617006No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGT108180.1657023924986965No Hit
GATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTT103970.15925381538259822No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT101650.15570020519035402No Hit
GTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGC101030.1547505335010474No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG100380.15375490995580657No Hit
GTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA100050.15324943954053044No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA99260.15203937400093007No Hit
GCTTCTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT99000.15164112458283374No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG94050.14405906835369206No Hit
GCTTACACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT86670.13275491179388083No Hit
GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCA86520.13252515251420985No Hit
TGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGT86420.13237197966109587No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCA82140.12581618154781782No Hit
ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGT81150.12429977030198947No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTAT80350.12307438747707768No Hit
ATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGT80340.12305907019176628No Hit
GTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGCTT80220.12287526276802951No Hit
TACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGA78970.12096060210410485No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA78640.12045513168882874No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG77550.11878554758988642No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT77500.11870896116332945No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAG77390.11854047102490407No Hit
CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAG75970.11636541651068565No Hit
CCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA70850.1085229664312502No Hit
AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAC67070.102733032583542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTC81550.040.2897694-95
GTACCCG1001.2862911E-633.296551
GCTTACG902.3917013E-531.7111
TGCACCG902.4289739E-531.6393365
GCACCGT902.4331739E-531.6313386
CCGGACA650.00587076729.2055424
TTATGTA5600.028.8143943
CTTATGT6050.028.2400682
CATTCCG856.4535474E-427.9091519
AAACGTT350.00843060427.11911636-37
TACGCTT1250.026.56971494-95
GCTTCCC22350.026.1775381
TATGTAC6400.025.9541424
TACCTGG7500.025.9442562
ACACTCT83000.025.6736055
TACACTC85200.024.8992674
GCTTACA90100.024.6015421
TTACACT86850.024.3169333
CTCCGAC3250.024.08792994-95
CTTACAC88800.024.050172
Produced by FastQC (version 0.11.5)