##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938860_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6528572 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00852866446139 31.0 31.0 34.0 30.0 34.0 2 32.07101798065488 33.0 31.0 34.0 30.0 34.0 3 32.28127605853164 34.0 31.0 34.0 30.0 34.0 4 35.617237582736315 37.0 35.0 37.0 33.0 37.0 5 35.60911880882986 37.0 35.0 37.0 33.0 37.0 6 35.73122269310961 37.0 35.0 37.0 33.0 37.0 7 35.783808158966465 37.0 35.0 37.0 33.0 37.0 8 35.82532964329719 37.0 35.0 37.0 33.0 37.0 9 37.378940295059934 39.0 37.0 39.0 34.0 39.0 10-11 37.20505648095786 39.0 37.0 39.0 33.5 39.0 12-13 37.0267033127612 39.0 37.0 39.0 32.5 39.0 14-15 37.78703803833365 39.0 37.0 41.0 32.5 41.0 16-17 37.63304770170261 39.0 36.5 40.5 32.5 41.0 18-19 38.48826772531574 40.0 38.0 41.0 33.5 41.0 20-21 38.55909431649065 40.0 38.0 41.0 34.0 41.0 22-23 38.51358290909559 40.0 38.0 41.0 34.0 41.0 24-25 38.49361552572293 40.0 38.0 41.0 33.5 41.0 26-27 37.81765874681324 39.5 37.0 41.0 32.5 41.0 28-29 37.423260400589896 39.0 36.5 40.5 31.5 41.0 30-31 37.70128697362915 39.5 37.0 41.0 32.5 41.0 32-33 38.030333356207144 40.0 37.5 41.0 33.5 41.0 34-35 38.307386822110566 40.0 38.0 41.0 34.0 41.0 36-37 37.84858013360349 40.0 37.0 41.0 33.0 41.0 38-39 37.24356574454566 39.0 36.5 41.0 30.5 41.0 40-41 36.888241946324555 39.0 35.5 40.0 30.5 41.0 42-43 37.39281967021272 39.0 37.0 40.5 32.0 41.0 44-45 37.785289570215355 40.0 37.0 41.0 33.0 41.0 46-47 37.411068147827734 40.0 37.0 41.0 31.0 41.0 48-49 37.45570225770659 40.0 37.0 41.0 31.5 41.0 50-51 37.400704089653914 40.0 37.0 41.0 31.5 41.0 52-53 36.608230176522525 39.0 35.5 40.0 29.5 41.0 54-55 36.162194504403104 38.0 34.5 40.0 28.5 41.0 56-57 36.73585165331714 39.0 36.0 40.0 31.0 41.0 58-59 36.89592249269825 39.0 36.0 40.5 31.0 41.0 60-61 36.41023550019821 38.5 35.5 40.5 29.5 41.0 62-63 35.52208790835117 38.0 34.5 40.0 28.0 41.0 64-65 34.32442546394525 36.5 32.0 39.0 26.0 40.5 66-67 33.88251711093942 36.0 32.0 39.0 26.0 39.5 68-69 34.325339676119064 36.0 33.5 39.0 28.0 39.0 70-71 33.78949278647765 36.0 33.5 38.0 27.0 39.0 72-73 32.680846975418206 35.0 32.0 37.0 26.0 37.5 74-75 32.265373806094196 35.0 31.5 36.0 26.0 37.0 76-77 30.022993542845207 32.5 29.0 34.0 21.5 36.0 78-79 30.73143177405411 34.0 29.5 35.0 23.0 36.0 80-81 31.067318243560766 34.0 31.0 35.0 25.0 36.0 82-83 31.16018740392233 34.0 31.0 35.0 25.0 36.0 84-85 30.99598120385285 34.0 31.0 35.0 24.5 35.5 86-87 30.112299136779068 34.0 30.0 35.0 20.0 35.0 88-89 28.96790584525988 33.5 29.0 34.5 14.5 35.0 90-91 28.25174456221054 32.5 27.0 34.0 8.5 35.0 92-93 28.476179783266538 33.0 28.5 34.5 6.0 35.0 94-95 28.626438599436447 34.0 29.0 35.0 2.0 35.0 96-97 27.426988781007548 33.0 26.5 34.0 2.0 35.0 98-99 26.664398202240857 33.0 25.0 34.0 2.0 35.0 100-101 23.114265646453774 28.5 13.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.037887125715137415 1101 2 0.08719032513533165 1101 3 -0.011336594582594728 1101 4 -1.279232630650995E-4 1101 5 -0.1414723915948315 1101 6 0.045776837891459365 1101 7 0.13515697770081658 1101 8 0.1410758105840486 1101 9 0.19867839439035606 1101 10-11 0.17290070472674302 1101 12-13 0.16703444236829768 1101 14-15 0.27295370124998186 1101 16-17 0.2686120278589428 1101 18-19 0.2271782242495064 1101 20-21 0.20390806806744877 1101 22-23 0.10921783291760079 1101 24-25 0.15065848895736167 1101 26-27 0.12211452058367911 1101 28-29 0.0977049370326597 1101 30-31 0.17273890210238818 1101 32-33 0.19945167964184662 1101 34-35 0.28222128646199707 1101 36-37 0.29868032080278795 1101 38-39 0.36366283504008123 1101 40-41 0.2976981398935976 1101 42-43 0.32956285120847184 1101 44-45 0.3928569619405735 1101 46-47 0.37415117097337003 1101 48-49 0.2730460290540364 1101 50-51 0.2547680773304819 1101 52-53 0.28804560446805993 1101 54-55 0.31247813100033994 1101 56-57 0.27758787155698883 1101 58-59 0.4135937431098 1101 60-61 0.5210904909774356 1101 62-63 0.3181259468496975 1101 64-65 0.32489925200098213 1101 66-67 0.21736458383917778 1101 68-69 0.25463372757036495 1101 70-71 0.31336445488979336 1101 72-73 0.2378399550522161 1101 74-75 0.23668987017926923 1101 76-77 0.21722903050447684 1101 78-79 0.11785410449923361 1101 80-81 0.24816311752693565 1101 82-83 0.31705426231295064 1101 84-85 0.39788014428510365 1101 86-87 0.3445906097683107 1101 88-89 0.4441393718139999 1101 90-91 0.2717137440540469 1101 92-93 0.30036014544311485 1101 94-95 0.5550353356173403 1101 96-97 0.655381671075407 1101 98-99 0.5770828223482631 1101 100-101 1.0970484672918168 1102 1 0.21353768787005123 1102 2 0.18548905281571137 1102 3 0.16260781342210606 1102 4 0.13489450876420506 1102 5 -0.08199350230162139 1102 6 -0.04025961896466157 1102 7 0.10563081019182619 1102 8 0.108651705344748 1102 9 0.13271051041463267 1102 10-11 0.23141575510197043 1102 12-13 0.26878954387338894 1102 14-15 0.310469625035374 1102 16-17 0.3845334714933628 1102 18-19 0.3111202782109572 1102 20-21 0.24093857862730061 1102 22-23 0.1972404645931718 1102 24-25 0.2611396501745773 1102 26-27 0.3147731790547468 1102 28-29 0.3493577482919292 1102 30-31 0.33510479458080766 1102 32-33 0.3279414291667919 1102 34-35 0.35563895092688824 1102 36-37 0.31325050772464635 1102 38-39 0.37338937100609115 1102 40-41 0.3896530373186664 1102 42-43 0.39135533790438615 1102 44-45 0.3658619968359105 1102 46-47 0.4082947601839706 1102 48-49 0.38544127074541734 1102 50-51 0.41450605931643736 1102 52-53 0.33409657965542294 1102 54-55 0.3030005022958804 1102 56-57 0.25181808540477846 1102 58-59 0.31652493142573945 1102 60-61 0.2785080642896389 1102 62-63 0.1470217350007843 1102 64-65 0.28720011006060275 1102 66-67 0.40936027649712514 1102 68-69 0.3311536993722015 1102 70-71 0.28532257161615604 1102 72-73 0.3315463783798194 1102 74-75 0.2724918024462113 1102 76-77 0.35184316397372584 1102 78-79 0.3796906472768278 1102 80-81 0.36987680121623256 1102 82-83 0.4237431445087765 1102 84-85 0.5669798154335339 1102 86-87 0.6097480720964548 1102 88-89 0.631109879293664 1102 90-91 0.6639281181714196 1102 92-93 0.742536325767297 1102 94-95 0.8871781076642726 1102 96-97 0.9800243335093484 1102 98-99 1.29677206031025 1102 100-101 1.763357169770181 1103 1 0.1791006038541454 1103 2 0.1630682963288166 1103 3 0.10676328950646763 1103 4 0.04997387477690296 1103 5 -0.03245153315897653 1103 6 0.04126513045998337 1103 7 0.04723084688290413 1103 8 0.03931458687215894 1103 9 0.1066155503024575 1103 10-11 0.1551445719833211 1103 12-13 0.16901196392428375 1103 14-15 0.19154310557989618 1103 16-17 0.10434418317088756 1103 18-19 9.029110230471815E-4 1103 20-21 0.10070144294596872 1103 22-23 0.2071186353235035 1103 24-25 0.19281486829243732 1103 26-27 0.2254317799565868 1103 28-29 0.10067535385423554 1103 30-31 0.22395925291899488 1103 32-33 0.26196981535919406 1103 34-35 0.15773900545390518 1103 36-37 0.1985857979153849 1103 38-39 0.20778639275692257 1103 40-41 0.2334897792765105 1103 42-43 0.24535621362943516 1103 44-45 0.2619860706900283 1103 46-47 0.34919507923169846 1103 48-49 0.28314666727364113 1103 50-51 0.35568802941556044 1103 52-53 0.3596368616357566 1103 54-55 0.2563804913120791 1103 56-57 0.19022568484798796 1103 58-59 0.2909899414275827 1103 60-61 0.31930081889964157 1103 62-63 0.22948865325220424 1103 64-65 0.2429581590674985 1103 66-67 0.33648046995631375 1103 68-69 0.23757577454265544 1103 70-71 0.2182633574715922 1103 72-73 0.29045315678178696 1103 74-75 0.398885308752881 1103 76-77 0.38299255506257524 1103 78-79 0.31675702323727606 1103 80-81 0.39755132000844995 1103 82-83 0.47493601609118485 1103 84-85 0.5525752359481615 1103 86-87 0.5109965486915868 1103 88-89 0.394482228255594 1103 90-91 0.3021531246296014 1103 92-93 0.3431433749227537 1103 94-95 0.559653998765473 1103 96-97 0.6679606525398256 1103 98-99 0.9593664486923466 1103 100-101 1.6337549582704298 1104 1 0.0766137984438302 1104 2 0.07954566252438866 1104 3 0.029874164733435293 1104 4 0.02293400331939921 1104 5 -0.092836207572077 1104 6 -0.02086153053478057 1104 7 0.02060124493394966 1104 8 -0.025358140684154762 1104 9 0.04663726761427256 1104 10-11 0.03806135810726374 1104 12-13 -0.054347942567702034 1104 14-15 -0.02827453173479455 1104 16-17 0.13697767134843275 1104 18-19 0.1368955335827664 1104 20-21 0.1417758751070295 1104 22-23 0.08924543074809321 1104 24-25 0.07600934681111227 1104 26-27 0.1479070864458336 1104 28-29 0.07496775959415203 1104 30-31 -0.0343791667738671 1104 32-33 -0.030436743196588623 1104 34-35 -0.0018861662565043957 1104 36-37 0.11317166742693985 1104 38-39 -0.008180691581507915 1104 40-41 0.037278365270282166 1104 42-43 0.10777955954143437 1104 44-45 0.10757242475218476 1104 46-47 0.019645083958288012 1104 48-49 0.14896277226058174 1104 50-51 0.062223137532910755 1104 52-53 0.07915927035639214 1104 54-55 0.05935599225063015 1104 56-57 0.01869369231435769 1104 58-59 0.27247968593938054 1104 60-61 0.2477171053496292 1104 62-63 0.13374402098802562 1104 64-65 -0.028606217268126954 1104 66-67 -0.03802441247488986 1104 68-69 0.11033225050595519 1104 70-71 0.01736706379687547 1104 72-73 0.03735327808747968 1104 74-75 0.0015280441517973031 1104 76-77 0.08251382974692945 1104 78-79 0.021077615293837226 1104 80-81 0.030723716703647597 1104 82-83 0.057236372493967735 1104 84-85 0.1314447448234155 1104 86-87 0.2991218617687572 1104 88-89 0.36546093862022744 1104 90-91 0.25746593380205596 1104 92-93 0.2557167178143942 1104 94-95 0.32319287100430927 1104 96-97 0.35183817248520555 1104 98-99 0.7228621913598658 1104 100-101 1.5135952832045696 1105 1 0.07692174181648426 1105 2 0.11193946444946334 1105 3 0.06495212295853037 1105 4 0.08861518660135914 1105 5 -0.01908304315922038 1105 6 -0.02420794540593363 1105 7 0.0016502666014588385 1105 8 0.10984950793808679 1105 9 0.10391750701023739 1105 10-11 0.13278153145554228 1105 12-13 0.07239318761623537 1105 14-15 0.04063019319092831 1105 16-17 0.05743353250230854 1105 18-19 0.11518829789316243 1105 20-21 0.055118545782939066 1105 22-23 0.005693250925098425 1105 24-25 -0.004354695974548406 1105 26-27 0.09507184088695908 1105 28-29 0.16546354842412114 1105 30-31 0.14104125182564786 1105 32-33 0.18274599177870954 1105 34-35 0.1731050774616918 1105 36-37 0.08405376720780566 1105 38-39 0.24331678466618456 1105 40-41 0.23638668592605683 1105 42-43 0.17444880182257094 1105 44-45 0.22481120617487704 1105 46-47 0.1270269592004709 1105 48-49 0.08902897712523128 1105 50-51 0.1282641398081097 1105 52-53 0.26669154964169905 1105 54-55 0.20946538723637076 1105 56-57 0.13820220371091807 1105 58-59 0.29443916767515077 1105 60-61 0.22027408119821956 1105 62-63 0.1494652003061816 1105 64-65 0.13704842399721429 1105 66-67 0.10568612807526989 1105 68-69 0.07668901988554211 1105 70-71 0.13301106349522485 1105 72-73 0.20104816471495468 1105 74-75 0.2500400952554358 1105 76-77 0.2649489087870762 1105 78-79 0.04303806591279269 1105 80-81 0.02768838061879464 1105 82-83 0.21836736544690893 1105 84-85 0.2327956182796811 1105 86-87 0.33353548908923614 1105 88-89 0.2128353405434389 1105 90-91 0.15220830803200158 1105 92-93 0.30659621883524935 1105 94-95 0.565486616362751 1105 96-97 0.6328309424097363 1105 98-99 0.7988911278097959 1105 100-101 1.5262459505955306 1106 1 0.1459749111373867 1106 2 0.06365806620218706 1106 3 0.06066157253694371 1106 4 0.09539454307098794 1106 5 -0.020207150850602318 1106 6 0.06594382940120624 1106 7 0.06297960400828373 1106 8 0.05660696599534987 1106 9 0.11907152658705655 1106 10-11 0.06785724353398592 1106 12-13 0.09585609804171469 1106 14-15 0.09329568237539121 1106 16-17 0.17187508346282954 1106 18-19 0.045011426256891696 1106 20-21 -0.018867365576326733 1106 22-23 0.13058645968097693 1106 24-25 0.15818266702608952 1106 26-27 0.17379008651685268 1106 28-29 0.2018779945254252 1106 30-31 0.1873718286696402 1106 32-33 0.16188762225375086 1106 34-35 0.1683168519729108 1106 36-37 0.06013952126090771 1106 38-39 0.2249197316282121 1106 40-41 0.3436350168409774 1106 42-43 0.19109516252898828 1106 44-45 0.14795535930485926 1106 46-47 0.17748117289159637 1106 48-49 0.24814917232999534 1106 50-51 0.3042712610485836 1106 52-53 0.31178111848580414 1106 54-55 0.30634845611927375 1106 56-57 0.234398692303337 1106 58-59 0.344106951895931 1106 60-61 0.39589791941589425 1106 62-63 0.30985462781593043 1106 64-65 0.35268472448705523 1106 66-67 0.2430990457063089 1106 68-69 0.16801197667388124 1106 70-71 0.21970938935308482 1106 72-73 0.18681880782981608 1106 74-75 0.31130695408383957 1106 76-77 0.3177964624745684 1106 78-79 0.1607499878795302 1106 80-81 0.0822719824292868 1106 82-83 0.34357468994290485 1106 84-85 0.4355000184944515 1106 86-87 0.43616615647396273 1106 88-89 0.37296998922735725 1106 90-91 0.28753077755837 1106 92-93 0.44282733870068114 1106 94-95 0.7320297277979826 1106 96-97 0.8776204164566543 1106 98-99 1.113935077663971 1106 100-101 1.7105211717230802 1107 1 0.029595147017559498 1107 2 -0.026637961546953193 1107 3 -0.04563920354064521 1107 4 -0.07840819815485389 1107 5 -0.1499091774487269 1107 6 -0.07827144089245763 1107 7 -0.01866441016574072 1107 8 -0.08023100519183402 1107 9 -0.08976846277618478 1107 10-11 -0.11134702348910963 1107 12-13 -0.011307549678946316 1107 14-15 -0.08757384308808724 1107 16-17 -0.0022775048401726394 1107 18-19 -0.06500154021237137 1107 20-21 -0.10078222123930658 1107 22-23 -0.11100595180547401 1107 24-25 -0.15855468389168692 1107 26-27 -0.1250683144139444 1107 28-29 -0.0984230260259693 1107 30-31 -0.06792529862674002 1107 32-33 -0.1099284476681035 1107 34-35 -0.11959184232645015 1107 36-37 -0.14699002879812895 1107 38-39 -0.13014229921214593 1107 40-41 -0.26912657402451146 1107 42-43 -0.18290900639265573 1107 44-45 -0.1737047562177878 1107 46-47 -0.36094395768937915 1107 48-49 -0.2054247850886739 1107 50-51 -0.302162279509119 1107 52-53 -0.34825393640577573 1107 54-55 -0.3018150679997973 1107 56-57 -0.3756839706441042 1107 58-59 -0.1863902774010029 1107 60-61 -0.2483382110824266 1107 62-63 -0.3184952871056268 1107 64-65 -0.2221172887761398 1107 66-67 -0.19761591148932922 1107 68-69 -0.2989551581711609 1107 70-71 -0.3804230534509614 1107 72-73 -0.37001194416691163 1107 74-75 -0.23412959346260465 1107 76-77 -0.344099000996291 1107 78-79 -0.2517224941962901 1107 80-81 -0.21020524290615583 1107 82-83 -0.1986405156256339 1107 84-85 -0.12295670847315421 1107 86-87 -0.15683014678584328 1107 88-89 -0.24632240533697924 1107 90-91 -0.12321800826074991 1107 92-93 0.04346822462382249 1107 94-95 0.21099308870057953 1107 96-97 0.18133836777400347 1107 98-99 0.3615722208374983 1107 100-101 1.062698986836942 1108 1 0.19387522004436875 1108 2 0.06644471418444908 1108 3 0.1303846173633474 1108 4 0.11066870391685057 1108 5 0.03472440342908101 1108 6 0.09269428474060248 1108 7 0.05612002382101622 1108 8 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-1.083336066973807 2316 54-55 -1.0864921294476417 2316 56-57 -0.9740004188032998 2316 58-59 -0.9133744470078256 2316 60-61 -0.7958809255202723 2316 62-63 -0.9272020256078761 2316 64-65 -0.9992757813416162 2316 66-67 -0.9671885560755911 2316 68-69 -0.9072581571869733 2316 70-71 -0.8866816135477364 2316 72-73 -0.9631507762522276 2316 74-75 -1.0548619735344644 2316 76-77 -0.9955337889328746 2316 78-79 -0.8773372875419057 2316 80-81 -0.9731762002928939 2316 82-83 -1.0067938371953886 2316 84-85 -1.248549795594169 2316 86-87 -1.4702800189629777 2316 88-89 -2.0158273535773255 2316 90-91 -2.581232615835944 2316 92-93 -2.332493330527676 2316 94-95 -2.230345435977867 2316 96-97 -2.3852198339113713 2316 98-99 -2.5379159981253245 2316 100-101 -2.4247960117373673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 16.0 5 55.0 6 113.0 7 400.0 8 933.0 9 1630.0 10 2717.0 11 4426.0 12 6713.0 13 10041.0 14 12095.0 15 14304.0 16 16998.0 17 21491.0 18 24539.0 19 26242.0 20 29225.0 21 32853.0 22 37944.0 23 44071.0 24 52573.0 25 64174.0 26 79183.0 27 98555.0 28 122242.0 29 151092.0 30 185412.0 31 227941.0 32 282605.0 33 357074.0 34 470463.0 35 655097.0 36 1055763.0 37 1656192.0 38 775794.0 39 7591.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 70.78582531500793 9.27538390091691 6.638766604362327 13.300024179712839 2 7.853601063142139 26.403048017238685 34.092340560845464 31.65101035877371 3 32.43337134062395 24.852387321454064 36.36037099690407 6.353870341017913 4 6.3748090700385935 10.987180657577184 61.866974891293225 20.771035381090996 5 9.052080885126538 59.432767785433974 23.22461277858997 8.290538550849517 6 13.195764863241589 33.955523435754 36.854255532457955 15.994456168546446 7 21.93763046497764 56.24910010948796 9.411859132441213 12.40141029309319 8 11.061025290063432 27.819713101119202 16.300624393818435 44.81863721499893 9 26.149516310764437 27.09105452157072 29.623721695954337 17.135707471710507 10-11 17.45261444616066 19.102446905693927 30.54054546691068 32.90439318123473 12-13 46.904323028068006 11.428571209753068 20.086827563516188 21.58027819866274 14-15 41.131521563980606 16.577315835683514 32.994106521303586 9.297056079032291 16-17 20.710676864577454 23.041195724033898 40.86205861286601 15.38606879852264 18-19 9.831361283907109 40.39660587338242 33.19240409694494 16.579628745765536 20-21 16.02821413319789 40.75931153091365 12.36626478194619 30.846209553942273 22-23 18.14448856503382 30.145037842885092 10.77336667191539 40.9371069201657 24-25 19.656964965859167 27.463151825842996 23.880508768721242 28.99937443957659 26-27 37.727470531821865 18.44384062882602 21.510954382511223 22.317734456840892 28-29 19.56698187597533 18.281892272919713 48.62260077701525 13.528525074089709 30-31 12.95563562751548 43.62802462774402 31.669038803585224 11.747300941155277 32-33 15.908831211480855 40.91552486516194 14.328209905627142 28.847434017730063 34-35 23.60834733105331 18.92932396661135 22.750676627738056 34.71165207459728 36-37 37.025527297428425 20.395280998816848 29.63092358877074 12.948268114983986 38-39 40.893151821501384 9.960166625508679 30.18895499358732 18.95772655940262 40-41 20.146313430500314 18.134231857170615 50.17772178343562 11.54173292889344 42-43 18.31545396634572 38.121183252807825 27.521265712278336 16.042097068568122 44-45 11.815711606623008 42.129009044115854 21.010118509579705 25.04516083968143 46-47 15.665004680224484 24.116152893766486 10.66287786245394 49.55596456355509 48-49 24.106827137544904 27.011910247963012 15.055579395640944 33.82568321885115 50-51 39.68408247379097 26.8219032924663 22.166297954023936 11.327716279718793 52-53 19.891861497430067 24.943800880192484 35.68484808010082 19.479489542276625 54-55 10.53653846507322 32.654185325673055 41.61663530707787 15.192640902175853 56-57 16.42148456892193 42.638969156837554 25.58877784893607 15.350768425304448 58-59 18.010678942595053 26.16101219428697 24.189119205507993 31.639189657609982 60-61 17.91413909134808 18.39587985278186 30.189942953162774 33.500038102707286 62-63 43.45143573998069 12.030308577065382 22.119348733977024 22.39890694897691 64-65 37.455257469444575 16.503368939462636 34.059902911312676 11.981470679780113 66-67 19.755109743673955 22.86224228019116 40.06522022683318 17.3174277493017 68-69 10.140756229956805 38.54946342273365 33.317158241641934 17.992622105667618 70-71 15.19321462329647 38.813245291579214 14.391921108592099 31.601618976532226 72-73 17.373316860103557 29.74370965044117 12.22278777043433 40.660185719020944 74-75 19.218907697084354 26.79002202177915 24.330251938527727 29.660818342608763 76-77 33.536701806139476 18.877995161885565 24.0216182548703 23.56368477710466 78-79 17.978888876788794 18.663952018584933 46.1484064198748 17.20875268475147 80-81 13.513652819443347 39.931523373268455 30.682859245723176 15.871964561565013 82-83 16.62556108676194 37.85399999555795 16.077909619088633 29.44252929859148 84-85 22.17741490788491 18.298488551554613 23.37871896028718 36.1453775802733 86-87 32.53096342117773 19.249311112608623 29.27306251930506 18.946662946908578 88-89 35.77415534495241 12.285525524759352 30.11631194468103 21.824007185607204 90-91 19.310005353646645 18.835457315507526 45.31305302693213 16.5414843039137 92-93 17.530583246605076 34.21357951215231 28.419820469635333 19.836016771607284 94-95 12.661869787318777 37.643205612272254 23.01939940721906 26.675525193189912 96-97 14.875149717177274 24.815788230160113 14.712150513447874 45.59691153921474 98-99 21.351307759185318 26.60019679648168 16.959099172070093 35.08939627226291 100-101 32.271584934740524 25.19579987798073 24.180892419472414 18.351722767806336 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 83.0 1 58.0 2 56.5 3 101.5 4 97.0 5 91.0 6 125.0 7 204.5 8 330.5 9 397.0 10 445.5 11 509.5 12 551.5 13 623.5 14 736.5 15 826.5 16 915.5 17 1038.0 18 1175.0 19 1333.5 20 1493.0 21 1693.5 22 1923.5 23 2143.5 24 2457.5 25 2962.0 26 3571.5 27 4250.5 28 4807.0 29 5363.0 30 6094.5 31 6856.0 32 7597.5 33 8362.5 34 9460.0 35 10232.5 36 10961.5 37 12057.5 38 12378.0 39 12379.0 40 13721.0 41 20942.5 42 58329.5 43 182589.0 44 574914.5 45 1125893.5 46 1403893.0 47 1225837.5 48 798171.0 49 443949.0 50 245000.0 51 127590.5 52 54166.5 53 23608.5 54 15117.5 55 12302.0 56 10692.5 57 9478.5 58 8636.5 59 7686.0 60 6554.0 61 5405.5 62 4290.5 63 3340.0 64 2494.0 65 1853.0 66 1316.0 67 1042.5 68 790.5 69 506.5 70 382.0 71 298.5 72 242.0 73 230.5 74 186.0 75 149.5 76 109.0 77 57.0 78 33.0 79 21.5 80 13.0 81 11.5 82 8.0 83 4.5 84 3.5 85 3.0 86 2.5 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22741573501831638 2 0.0 3 0.0 4 0.0 5 0.025824943035015927 6 0.003599562048178377 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002695842214805933 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.9824941809633104E-4 26-27 0.0013632383927143638 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006517504899999571 36-37 0.01668052370411171 38-39 0.013479211074029666 40-41 0.00814113714300769 42-43 0.00791137786333673 44-45 0.0036608311894239663 46-47 5.973741271444966E-4 48-49 0.0018150983094005857 50-51 1.5317285311397347E-5 52-53 0.0 54-55 0.0 56-57 0.0013938729633371585 58-59 3.5229756216213895E-4 60-61 0.0025196934337248637 62-63 8.730852627496489E-4 64-65 0.0016542668136309135 66-67 0.005858861631609485 68-69 2.3741792232665886E-4 70-71 4.595185593419204E-5 72-73 0.0 74-75 5.361049858989071E-4 76-77 0.05691903221715254 78-79 0.13603281085051983 80-81 0.0010262581158636222 82-83 8.960611907167449E-4 84-85 0.0 86-87 0.10174506768095687 88-89 0.34054154568564154 90-91 0.37671331494850635 92-93 5.897154844887979E-4 94-95 3.369802768507416E-4 96-97 1.9146606639246683E-4 98-99 0.0 100-101 0.0014551421045827478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6528572.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.976403678790824 #Duplication Level Percentage of deduplicated Percentage of total 1 75.95202147329127 8.33680047911034 2 11.054770072443496 2.4268323778271097 3 3.700501134454077 1.2185458279677404 4 1.991950272415234 0.8745780119242784 5 1.236156904647901 0.6784278597869949 6 0.8785860444784133 0.5786229054448281 7 0.6269330166602175 0.4817028909297248 8 0.4980956190965666 0.43738388686729174 9 0.3907936897943347 0.38605583648760977 >10 2.7346680777977963 6.034392038552818 >50 0.42738357757067463 3.294482695156451 >100 0.37233039035081433 8.479731157533568 >500 0.0716113081602851 5.48782663210599 >1k 0.049652371868947685 9.778178638156922 >5k 0.005314901777521161 4.099170576329603 >10k+ 0.009231145192536752 47.40726818581878 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 383698 5.877211739412539 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 231113 3.540023760172975 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 230305 3.5276473936413657 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 211674 3.242271051004722 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 182252 2.791605882572789 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 149435 2.2889385305086623 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 119891 1.8364046532687393 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 113929 1.7450829982421885 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 111797 1.7124265459582892 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 111449 1.7070961306699228 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 97812 1.4982143108783974 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 90795 1.390732919848322 No Hit CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 67577 1.0350961894882986 No Hit CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA 38386 0.5879693139632985 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 38175 0.5847373667625938 No Hit GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT 37398 0.572835836075638 No Hit CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 37398 0.572835836075638 No Hit GCTTTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 35535 0.5442997335405048 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 32531 0.4982866084650671 No Hit GAGTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 30504 0.4672384711388647 No Hit GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG 29821 0.4567767652711803 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA 28970 0.4437417554711811 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 26325 0.4032275358225351 No Hit TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC 24828 0.3802975597113733 No Hit ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA 23229 0.355805220498449 No Hit TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG 22723 0.3480546741308819 No Hit GCTTTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 22598 0.3461400134669572 No Hit CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 21456 0.32864767364134145 No Hit GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT 21006 0.32175489525121265 No Hit GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT 20582 0.3152603662791802 No Hit GCTTATACTCTGCGTTGATACCACTGCTTTTACTCTGCGTTGATACCACT 20500 0.3140043488836456 No Hit AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG 20424 0.3128402351999794 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT 19547 0.299406975981884 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 19091 0.2924222938798867 No Hit GAGTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18159 0.2781465839696644 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC 17818 0.27292338967847796 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 17311 0.2651575260255995 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG 16598 0.25423630159857313 No Hit GTAAAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 16582 0.2539912250335908 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG 16169 0.24766518619998368 No Hit GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCA 16128 0.2470371775022164 No Hit GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 15686 0.2402669373945788 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 15185 0.23259297745356874 No Hit ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTAT 14507 0.2222078580124413 No Hit TGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13699 0.20983149148083224 No Hit CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAG 13504 0.20684462084510977 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 12771 0.1956170507118555 No Hit CCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATA 12374 0.18953608844323078 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG 12224 0.18723849564652117 No Hit TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 11996 0.18374615459552257 No Hit ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 11906 0.18236759891749682 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 11767 0.18023849625921257 No Hit AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAT 11599 0.17766519232689781 No Hit GTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCAGA 11554 0.17697591448788494 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA 11358 0.17397372656685106 No Hit AGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 11074 0.1696236175384142 No Hit ATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG 11042 0.1691334644084495 No Hit GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 10928 0.1673872938829502 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 10910 0.16711158274734506 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA 10842 0.16607000734617006 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGT 10818 0.1657023924986965 No Hit GATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTT 10397 0.15925381538259822 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 10165 0.15570020519035402 No Hit GTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGC 10103 0.1547505335010474 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTG 10038 0.15375490995580657 No Hit GTAAAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10005 0.15324943954053044 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAAAGCAGTGGTATCAACGCA 9926 0.15203937400093007 No Hit GCTTCTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 9900 0.15164112458283374 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 9405 0.14405906835369206 No Hit GCTTACACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 8667 0.13275491179388083 No Hit GTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCA 8652 0.13252515251420985 No Hit TGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGT 8642 0.13237197966109587 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCA 8214 0.12581618154781782 No Hit ACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGT 8115 0.12429977030198947 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTAT 8035 0.12307438747707768 No Hit ATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGT 8034 0.12305907019176628 No Hit GTATAAGCAGTGGTATCAAGCCCATGTACTCTGCGTTGATACCACTGCTT 8022 0.12287526276802951 No Hit TACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGA 7897 0.12096060210410485 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 7864 0.12045513168882874 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7755 0.11878554758988642 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 7750 0.11870896116332945 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTATAAG 7739 0.11854047102490407 No Hit CAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTACAAG 7597 0.11636541651068565 No Hit CCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 7085 0.1085229664312502 No Hit AAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTAC 6707 0.102733032583542 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.0634570622794694E-5 0.0 9 0.0 0.0 0.0 1.3785556780257613E-4 0.0 10-11 0.0 0.0 0.0 3.216629915393443E-4 0.0 12-13 0.0 0.0 0.0 6.203500551115926E-4 0.0 14-15 0.0 0.0 0.0 6.969364816685794E-4 0.0 16-17 0.0 0.0 0.0 9.343544039952382E-4 0.0 18-19 0.0 0.0 0.0 0.0012100655396003904 0.0 20-21 0.0 0.0 0.0 0.001899343378613271 0.0 22-23 0.0 0.0 0.0 0.0031094089182136615 0.0 24-25 0.0 0.0 0.0 0.00467177201997619 0.0 26-27 0.0 0.0 0.0 0.006218817836427323 0.0 28-29 0.0 0.0 0.0 0.011694747335251873 0.0 30-31 0.0 0.0 0.0 0.032196933724557224 0.0 32-33 0.0 0.0 0.0 0.07778117481127572 0.0 34-35 0.0 0.0 0.0 0.3446312608637846 0.0 36-37 0.0 0.0 0.0 0.7544299120849092 0.0 38-39 0.0 0.0 0.0 0.8986651292196823 0.0 40-41 0.0 0.0 0.0 1.030531638465502 0.0 42-43 0.0 0.0 0.0 1.194342346228241 0.0 44-45 0.0 0.0 0.0 1.3769473630680644 0.0 46-47 0.0 0.0 0.0 1.6714298318223344 0.0 48-49 0.0 0.0 0.0 2.0233368032090326 0.0 50-51 0.0 0.0 0.0 2.3126343708853945 0.0 52-53 0.0 0.0 0.0 2.5169056877981895 0.0 54-55 0.0 0.0 0.0 2.7302984481139214 0.0 56-57 0.0 0.0 0.0 2.935833441064907 0.0 58-59 0.0 0.0 0.0 3.796833365703863 0.0 60-61 0.0 0.0 0.0 5.656849614280121 0.0 62-63 0.0 0.0 0.0 6.7779600194345715 0.0 64-65 0.0 0.0 0.0 7.120301346144302 0.0 66-67 0.0 1.5317285311397347E-5 0.0 7.5469796457785865 0.0 68-69 0.0 3.0634570622794694E-5 0.0 7.920882238872452 0.0 70-71 0.0 3.0634570622794694E-5 0.0 8.433245432538694 0.0 72-73 0.0 3.0634570622794694E-5 0.0 9.22053092161655 0.0 74-75 0.0 3.0634570622794694E-5 0.0 9.891290162687952 0.0 76-77 0.0 3.0634570622794694E-5 0.0 10.374542855619882 0.0 78-79 0.0 3.0634570622794694E-5 0.0 10.811407149986245 0.0 80-81 0.0 3.0634570622794694E-5 0.0 11.305205793855073 0.0 82-83 0.0 3.0634570622794694E-5 0.0 11.890172919897338 0.0 84-85 0.0 3.0634570622794694E-5 0.0 14.637358675067075 0.0 86-87 0.0 3.0634570622794694E-5 0.0 18.007100174433244 0.0 88-89 0.0 3.0634570622794694E-5 0.0 18.816143867295942 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCACTC 8155 0.0 40.28976 94-95 GTACCCG 100 1.2862911E-6 33.29655 1 GCTTACG 90 2.3917013E-5 31.711 1 TGCACCG 90 2.4289739E-5 31.639336 5 GCACCGT 90 2.4331739E-5 31.631338 6 CCGGACA 65 0.005870767 29.205542 4 TTATGTA 560 0.0 28.814394 3 CTTATGT 605 0.0 28.240068 2 CATTCCG 85 6.4535474E-4 27.909151 9 AAACGTT 35 0.008430604 27.119116 36-37 TACGCTT 125 0.0 26.569714 94-95 GCTTCCC 2235 0.0 26.177538 1 TATGTAC 640 0.0 25.954142 4 TACCTGG 750 0.0 25.944256 2 ACACTCT 8300 0.0 25.673605 5 TACACTC 8520 0.0 24.899267 4 GCTTACA 9010 0.0 24.601542 1 TTACACT 8685 0.0 24.316933 3 CTCCGAC 325 0.0 24.087929 94-95 CTTACAC 8880 0.0 24.05017 2 >>END_MODULE