FastQCFastQC Report
Thu 26 May 2016
SRR938859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126242
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15571.2333454793175012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14821.173935774148065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.7152928502400152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.5917206634875873No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.39051979531376246No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.2819980672042585No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3450.2732846437794078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.24001520888452338No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2350.18615040953090098No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.1750605978992728No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1710.13545412778631516No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1650.13070135137276023No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.12357218675242787No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1430.11327450452305889No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.11169024571854058No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1350.10693746930498566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCC200.00208611571.1089253
GGTATTA250.00506628856.842037
TATGCCA405.422549E-447.405954
AATCAAC500.001626115837.924763
GTGCATG702.066934E-433.8613931
GAATCAA650.005881285329.1728942
GTGTACA650.005881285329.1728941
TAAAGTG700.00844498627.0891135
TAAAGCT700.00844498627.0891134
CCGTATC458.865802E-426.42051594-95
GAGTACT13350.023.95029412-13
CATGGGG1601.8697174E-623.7029744
ATGGGAG1401.7904784E-523.7029745
ATGGGAT1954.2402826E-721.879675
GCCGTAT550.00285129321.61678594-95
GTACTTT15100.021.3229914-15
GTACATG9000.020.542581
AGTACTT13500.020.52628912-13
TAAATTG600.00472051219.81538630-31
GGTATCA22950.019.6233791
Produced by FastQC (version 0.11.5)