FastQCFastQC Report
Thu 26 May 2016
SRR938859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126242
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT14851.1763121623548423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14651.1604695743096594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14571.154132539091586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.7699497789958968No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT6860.5434007699497789RNA PCR Primer, Index 23 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6840.5418165111452606No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.3889355365092442No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG4140.3279415725352894RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT4060.32160453731721617RNA PCR Primer, Index 23 (96% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.2954642670426641No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3670.29071149062910917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.2669476085613346No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2140.16951569208345876No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1671393038766813No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT1800.14258329240664755RNA PCR Primer, Index 23 (96% over 28bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1640.12990922197050112No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1520.12040366914339126No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.11881941033887294No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1480.11723515153435465No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.11406663392531805No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1420.11248237512079973No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1390.11010598691402228No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1360.10772959870724481No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC1290.10218469289143076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGTC156.6424004E-494.862124
GGTATAG250.004945609357.1891751
CTTGGAC554.980633E-543.119153
TGGACTG459.661297E-442.1609425
ATGGGTA550.00258934634.495325
TTGGACT600.0039658531.6207074
AGAGTGA600.0039658531.6207076
TACCTGG650.00586612129.1883472
GGTATCA22350.026.4409281
TACACAG908.9791237E-426.350595
GAGTACT14000.025.92796112-13
CATGGGT1000.001659498923.7155324
GTACTTT15800.022.97414214-15
ACTTTTT16550.021.78966516-17
GTATCAA28600.020.730362
ATCAACG30850.019.0646724
TATCAAC30950.019.0030773
CAACGCA31150.018.8810656
AACGCAG30950.018.8498257
TCAACGC31450.018.7009585
Produced by FastQC (version 0.11.5)