##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938859_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 126242 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77821168866146 31.0 31.0 34.0 30.0 34.0 2 31.973637933492814 33.0 31.0 34.0 30.0 34.0 3 32.00136246257188 33.0 31.0 34.0 30.0 34.0 4 35.71356600814309 37.0 35.0 37.0 33.0 37.0 5 35.62214635382836 37.0 35.0 37.0 33.0 37.0 6 35.67933809667147 37.0 35.0 37.0 33.0 37.0 7 35.612838833351816 37.0 35.0 37.0 33.0 37.0 8 35.623896959807354 37.0 35.0 37.0 33.0 37.0 9 37.18178577652445 39.0 37.0 39.0 33.0 39.0 10-11 37.12010662061755 39.0 37.0 39.0 33.0 39.0 12-13 37.03801825066143 39.0 37.0 39.0 33.0 39.0 14-15 38.09002550656675 40.0 37.0 41.0 33.0 41.0 16-17 38.007513347380424 40.0 37.0 41.0 33.0 41.0 18-19 38.12041951173144 40.0 37.0 41.0 33.0 41.0 20-21 38.221653649340155 40.0 37.0 41.0 33.0 41.0 22-23 38.12767145640912 40.0 37.0 41.0 33.0 41.0 24-25 37.98329003025934 40.0 37.0 41.0 33.0 41.0 26-27 37.66334896468687 40.0 36.5 41.0 32.5 41.0 28-29 37.36229226406426 39.5 36.0 41.0 32.0 41.0 30-31 36.984058395779535 39.0 35.5 41.0 31.0 41.0 32-33 36.507794553318234 38.5 35.0 41.0 30.5 41.0 34-35 36.17391597091301 39.0 35.0 41.0 30.0 41.0 36-37 35.84923401086802 38.5 35.0 41.0 30.0 41.0 38-39 35.5016832749798 38.0 35.0 41.0 28.0 41.0 40-41 35.183995817556756 38.0 35.0 41.0 26.5 41.0 42-43 34.80404302846913 38.0 35.0 41.0 24.5 41.0 44-45 34.24700179021245 38.0 34.0 40.0 21.5 41.0 46-47 33.48430791654125 38.0 33.0 40.0 11.0 41.0 48-49 32.82174315996261 38.0 33.0 40.0 2.0 41.0 50-51 32.329379287400386 38.0 32.5 40.0 2.0 41.0 52-53 32.00918870106621 37.5 32.0 40.0 2.0 41.0 54-55 31.687655455395195 37.0 31.0 40.0 2.0 41.0 56-57 31.26151360086184 37.0 31.0 40.0 2.0 41.0 58-59 30.81327133600545 36.0 30.5 40.0 2.0 41.0 60-61 30.288842065239777 35.5 29.5 39.5 2.0 41.0 62-63 29.734973305239144 35.0 28.0 39.0 2.0 41.0 64-65 29.23077501940717 35.0 28.0 39.0 2.0 40.0 66-67 28.634709526148193 35.0 26.0 38.0 2.0 40.0 68-69 28.08720552589471 35.0 25.5 37.5 2.0 40.0 70-71 27.564633798577333 34.0 24.0 37.0 2.0 39.5 72-73 27.23031954500087 34.0 23.5 36.5 2.0 39.0 74-75 26.83170418719602 34.0 21.5 36.0 2.0 39.0 76-77 24.981396840987944 31.5 18.0 34.5 2.0 36.5 78-79 25.826072147145958 33.0 18.0 35.0 2.0 37.0 80-81 25.82724053801429 33.0 18.0 35.0 2.0 37.0 82-83 25.505176565643765 33.0 13.0 35.0 2.0 36.0 84-85 25.125607959316234 33.0 6.5 35.0 2.0 36.0 86-87 24.631176629014117 33.0 2.0 35.0 2.0 35.5 88-89 24.13428573691798 32.5 2.0 35.0 2.0 35.0 90-91 23.707363634923404 32.0 2.0 35.0 2.0 35.0 92-93 23.419254289380714 32.0 2.0 35.0 2.0 35.0 94-95 23.054260864054754 31.5 2.0 35.0 2.0 35.0 96-97 22.575672121797815 31.0 2.0 34.0 2.0 35.0 98-99 21.904421666323408 31.0 2.0 34.0 2.0 35.0 100-101 19.471015985171338 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16253571932756827 1101 2 0.1258267732481393 1101 3 0.12079288322568615 1101 4 -0.04868346519193523 1101 5 0.3661897709038371 1101 6 0.06111729853384418 1101 7 0.23686022680306706 1101 8 -0.1327797325741642 1101 9 0.15561279203793532 1101 10-11 0.10724735554961029 1101 12-13 0.1314587354523269 1101 14-15 0.4475748414666043 1101 16-17 0.3480833352802293 1101 18-19 0.711729476812188 1101 20-21 0.4197599506577916 1101 22-23 0.40415643295992254 1101 24-25 0.13633556038967498 1101 26-27 0.5359722727203646 1101 28-29 0.6218902909728428 1101 30-31 0.6227777185380745 1101 32-33 0.6539731735952046 1101 34-35 -0.10187574882382933 1101 36-37 -0.5067637852715947 1101 38-39 0.018385021561776682 1101 40-41 -0.27087664385541643 1101 42-43 -0.5518751366935604 1101 44-45 -0.05520577209586719 1101 46-47 0.07147251351073436 1101 48-49 -0.6836394643901329 1101 50-51 -0.3991569922914451 1101 52-53 -0.3993100797642839 1101 54-55 -0.1976336485772805 1101 56-57 -0.2577352196613596 1101 58-59 0.3285923223172027 1101 60-61 -0.05370633999425323 1101 62-63 0.457094941570503 1101 64-65 0.3183110195453409 1101 66-67 0.0331996087819455 1101 68-69 0.008328907130476182 1101 70-71 0.3846255717853282 1101 72-73 0.533047364009164 1101 74-75 0.6904259236281192 1101 76-77 0.1538625591116407 1101 78-79 -0.16350148542251475 1101 80-81 0.26742919591247727 1101 82-83 0.4886793751546783 1101 84-85 -0.382135029630831 1101 86-87 0.07292525342161582 1101 88-89 0.6429603345297821 1101 90-91 -0.026169205227315473 1101 92-93 0.1688322146747403 1101 94-95 0.08327194619782219 1101 96-97 -0.026151151210406454 1101 98-99 -0.1703312414848206 1101 100-101 -0.5747372060230056 1102 1 -0.10365677461916434 1102 2 0.29077834709801564 1102 3 0.49670087354045833 1102 4 0.3927511595054014 1102 5 -0.4723392847862371 1102 6 -0.1625509823378266 1102 7 0.023785166270378966 1102 8 -0.2269080618719883 1102 9 -0.14886662684826035 1102 10-11 0.024544571045971963 1102 12-13 0.07266636257097048 1102 14-15 0.18879760175715887 1102 16-17 0.3435433837063826 1102 18-19 0.549501873906614 1102 20-21 0.3621025656699004 1102 22-23 0.17889916903740755 1102 24-25 -0.12849494808489936 1102 26-27 0.45221016618283016 1102 28-29 0.1667601456943899 1102 30-31 -0.13176223303577927 1102 32-33 -0.281256850617865 1102 34-35 -0.056930228242720204 1102 36-37 -0.439496836119055 1102 38-39 -0.7768631624576017 1102 40-41 -0.2859341499086838 1102 42-43 -1.274408429671773 1102 44-45 -1.254282648609184 1102 46-47 -0.8844142903633596 1102 48-49 -0.7709578663271728 1102 50-51 -0.8939360479815193 1102 52-53 -0.6672428884809918 1102 54-55 -0.66040604567171 1102 56-57 -0.41829817365652033 1102 58-59 0.0773816685641755 1102 60-61 -0.7258461705027273 1102 62-63 -0.9571756395433013 1102 64-65 0.21903741179716008 1102 66-67 -0.10050196506793085 1102 68-69 0.10752684901909859 1102 70-71 0.5412539010831487 1102 72-73 0.3435043858009337 1102 74-75 0.24906696479035162 1102 76-77 0.2204485155281084 1102 78-79 0.5732569891537551 1102 80-81 0.29300425644516537 1102 82-83 0.7336097625638764 1102 84-85 0.9535187233958027 1102 86-87 0.873848376908299 1102 88-89 0.9775547655225161 1102 90-91 1.0413247415039173 1102 92-93 1.2538049749652949 1102 94-95 1.1733143190791786 1102 96-97 1.3815365001213102 1102 98-99 1.3988273541568255 1102 100-101 1.719981316979414 1103 1 -0.1827282558277119 1103 2 -0.1895458975593165 1103 3 -0.5080891043519493 1103 4 -0.37003439686895234 1103 5 -0.29444687505889533 1103 6 -0.16667773252205365 1103 7 -0.23767393468761355 1103 8 -0.04194122325739613 1103 9 -0.7484244750428104 1103 10-11 -0.4460523338913802 1103 12-13 -0.1813130036781132 1103 14-15 -0.42431956847128305 1103 16-17 -0.33033281378808965 1103 18-19 -0.21932642380892986 1103 20-21 -0.5315599251186001 1103 22-23 -0.2961929459220656 1103 24-25 -0.3234470483059724 1103 26-27 -0.7243926341119291 1103 28-29 -0.3376982183439239 1103 30-31 -0.344768865312858 1103 32-33 -0.7888839692619314 1103 34-35 -0.952729786090913 1103 36-37 -1.40268769831507 1103 38-39 -0.9983075250220779 1103 40-41 -0.8242539730479592 1103 42-43 -1.013870478308469 1103 44-45 -0.5116886913505212 1103 46-47 -0.3776346293464101 1103 48-49 0.15634500765956005 1103 50-51 -0.18343494260200544 1103 52-53 0.15018526185063052 1103 54-55 -0.024186443608332553 1103 56-57 0.04684552568025424 1103 58-59 0.14753642169608483 1103 60-61 0.07971757304922633 1103 62-63 -0.5902264870009262 1103 64-65 -0.9260989183428592 1103 66-67 -0.3180038073671234 1103 68-69 -0.6016478009440576 1103 70-71 -0.4654520679662255 1103 72-73 -0.05115294654984126 1103 74-75 0.6900765447461339 1103 76-77 0.19454578892530705 1103 78-79 0.30361807979487665 1103 80-81 0.8305503139248991 1103 82-83 0.5469480086950504 1103 84-85 0.38572211322631134 1103 86-87 0.7251379863408722 1103 88-89 0.7254913904304026 1103 90-91 0.701936384834795 1103 92-93 0.603731282997721 1103 94-95 0.46859803315434334 1103 96-97 0.6873581655597825 1103 98-99 0.5847153423661133 1103 100-101 1.4199444709956275 1104 1 0.26894672635333094 1104 2 0.23326237043783138 1104 3 -0.10110407227782403 1104 4 0.004448853309234835 1104 5 0.07183965380781387 1104 6 -0.08905834549425862 1104 7 0.019061631955295866 1104 8 -0.11784999018540532 1104 9 -0.0756518449644048 1104 10-11 0.03677018927326259 1104 12-13 -0.15184044487553905 1104 14-15 -0.1703993974092768 1104 16-17 -0.14694008392351066 1104 18-19 -0.03885600328148797 1104 20-21 0.2612412152947954 1104 22-23 0.18584554303018308 1104 24-25 0.2818273636683628 1104 26-27 -0.09495571322811003 1104 28-29 0.2939482535021156 1104 30-31 0.20972151244908588 1104 32-33 0.34718160450856317 1104 34-35 0.8271347898178547 1104 36-37 0.09210916554807369 1104 38-39 0.8484494243720704 1104 40-41 0.710314808135216 1104 42-43 1.0005985167022189 1104 44-45 1.332894345000156 1104 46-47 0.956791600161786 1104 48-49 1.4775139313944123 1104 50-51 1.1953248578256463 1104 52-53 1.1209621684792737 1104 54-55 1.071394454467221 1104 56-57 0.8331605648819611 1104 58-59 0.9468593012399182 1104 60-61 0.95295349607132 1104 62-63 1.1499374474253017 1104 64-65 0.8440721436671197 1104 66-67 0.7848828640512657 1104 68-69 0.5903693052569388 1104 70-71 0.23957287857689735 1104 72-73 0.2438933827445311 1104 74-75 1.3491642140262492 1104 76-77 1.2762278986900988 1104 78-79 0.8247157276922401 1104 80-81 0.35920320059631905 1104 82-83 1.0310534969345362 1104 84-85 1.0906401460131967 1104 86-87 1.583976190189766 1104 88-89 1.649546985583644 1104 90-91 1.4173319657328705 1104 92-93 1.2593620741595153 1104 94-95 1.684706372427332 1104 96-97 1.8851486145975578 1104 98-99 1.7662905383746654 1104 100-101 2.4522680632978364 1105 1 -0.5312499949581486 1105 2 -0.4914589410375747 1105 3 -0.2743499739171682 1105 4 -0.1804691794776474 1105 5 -0.3688816576675933 1105 6 -0.08702555860900674 1105 7 -0.11628263033978925 1105 8 -0.7386368754313111 1105 9 -0.2618157793906306 1105 10-11 -0.38657407302181923 1105 12-13 -0.1847912645476697 1105 14-15 -0.23771087281911463 1105 16-17 -0.32720237900547744 1105 18-19 -0.3054133803306698 1105 20-21 -0.31016862077078144 1105 22-23 -0.753236424182937 1105 24-25 -0.0726644396103282 1105 26-27 -0.23134915585107052 1105 28-29 -0.5610025661700107 1105 30-31 -1.3145079957476398 1105 32-33 -0.796883969261934 1105 34-35 -0.3619471773952583 1105 36-37 -1.0905137852715896 1105 38-39 -0.6028292641525113 1105 40-41 -0.7103409295697034 1105 42-43 -0.5936965652649846 1105 44-45 -0.5763843435244382 1105 46-47 -0.5176346293464036 1105 48-49 -0.4215680358187015 1105 50-51 -0.23247842086288273 1105 52-53 -0.3219886511928536 1105 54-55 -0.3074907914344216 1105 56-57 -0.13280664823279054 1105 58-59 -0.2585505348256554 1105 60-61 -0.5404563399942539 1105 62-63 -0.5982264870009288 1105 64-65 -0.8294032661689457 1105 66-67 -0.580264676932341 1105 68-69 -0.8847782357266674 1105 70-71 -0.7590172853575297 1105 72-73 -0.5386312074194066 1105 74-75 -1.3589669335147363 1105 76-77 -1.2092802980312136 1105 78-79 -1.185251485422512 1105 80-81 -1.5129279469446644 1105 82-83 -1.0123563391310384 1105 84-85 -1.7982778867736897 1105 86-87 -1.6322533180069527 1105 88-89 -1.450682522613075 1105 90-91 -1.5074549195130338 1105 92-93 -1.5347034996109734 1105 94-95 -1.3192280538021777 1105 96-97 -2.2042940083532656 1105 98-99 -2.0415455271991085 1105 100-101 -1.35727292030872 1106 1 0.40124587281044555 1106 2 0.04480552177234287 1106 3 0.12108804261175621 1106 4 0.30779528333226835 1106 5 0.07759768117538357 1106 6 -0.16689332720405048 1106 7 -0.0252413080257341 1106 8 0.06837138903149764 1106 9 0.024266865237464685 1106 10-11 -0.08604514740198255 1106 12-13 -0.02892349595263255 1106 14-15 -0.06319847612489582 1106 16-17 0.14102902595319478 1106 18-19 -0.47599189272737163 1106 20-21 -0.4992925877129295 1106 22-23 -0.38609592831516437 1106 24-25 -0.23659832390784885 1106 26-27 -0.3799441971733799 1106 28-29 0.04289826027626731 1106 30-31 -0.25989642549971137 1106 32-33 -0.4021071097578002 1106 34-35 -0.22207940880021226 1106 36-37 -0.12324105799886809 1106 38-39 -1.0085482724169808 1106 40-41 -0.5552996072556482 1106 42-43 -0.6790519371658164 1106 44-45 -0.8939380625327047 1106 46-47 -0.4858990921563233 1106 48-49 -0.9126093581327517 1106 50-51 -1.1766437101190732 1106 52-53 -1.4028150974738445 1106 54-55 -0.8353255021782253 1106 56-57 -0.7942611936873369 1106 58-59 -0.8735918571397043 1106 60-61 -0.5286546871016924 1106 62-63 -1.1613008671662186 1106 64-65 -0.47360161327638295 1106 66-67 0.24257829827427102 1106 68-69 0.00880854113284002 1106 70-71 -0.05981067378728255 1106 72-73 -0.4177717032871726 1106 74-75 -0.8518925533494439 1106 76-77 -0.7218753393535273 1106 78-79 -1.0465076837696223 1106 80-81 -1.0154072857876422 1106 82-83 -0.31212493417235976 1106 84-85 -0.5564266471042671 1106 86-87 -0.7782037312300929 1106 88-89 -0.45230235732381985 1106 90-91 0.3432227664373819 1106 92-93 0.2972634425377869 1106 94-95 0.15300335115649943 1106 96-97 0.4793092974318611 1106 98-99 1.4122395967678365 1106 100-101 1.534065922666489 1107 1 -0.02738558817848613 1107 2 -0.014306398664693631 1107 3 -0.35075675357818525 1107 4 -0.07335053540985115 1107 5 0.07850817284088407 1107 6 -0.27272047386324516 1107 7 -0.010113138814361378 1107 8 -0.06589111271944859 1107 9 -0.08954459294995587 1107 10-11 -0.14070966624215941 1107 12-13 0.04300534562182179 1107 14-15 0.019306076333428734 1107 16-17 0.034221349808078116 1107 18-19 -0.3869388040594899 1107 20-21 -0.2946770953470548 1107 22-23 -0.1346601529964957 1107 24-25 -0.09459664300015191 1107 26-27 -0.6664339016137788 1107 28-29 -0.4476466339666203 1107 30-31 -0.33515206354424976 1107 32-33 -0.20498566417718678 1107 34-35 0.5829002802318541 1107 36-37 0.5647404520165438 1107 38-39 0.990085990084772 1107 40-41 0.7394895789048732 1107 42-43 0.30610004490450393 1107 44-45 0.7711410802043801 1107 46-47 0.5096535062468135 1107 48-49 1.4070082353677407 1107 50-51 0.983182596086273 1107 52-53 0.637841857281721 1107 54-55 0.5684075136503246 1107 56-57 0.8571255551570367 1107 58-59 1.0477206516150233 1107 60-61 1.422458914243034 1107 62-63 1.3902819875753423 1107 64-65 1.1936136829835995 1107 66-67 0.4418709162879999 1107 68-69 0.7515946456292646 1107 70-71 0.9226098332865398 1107 72-73 1.1358772671568644 1107 74-75 0.914321202078483 1107 76-77 0.9068891934942123 1107 78-79 1.1876637688147724 1107 80-81 1.3523262903434699 1107 82-83 1.5386945083265893 1107 84-85 0.8560610962771591 1107 86-87 0.5475771904676208 1107 88-89 0.41399544348861994 1107 90-91 0.8252230465886647 1107 92-93 1.3343134495415647 1107 94-95 1.9021278784012132 1107 96-97 1.7671297204602965 1107 98-99 1.6318782016144553 1107 100-101 1.609811825453992 1108 1 0.012750005041851864 1108 2 0.02854105896242487 1108 3 -0.1543499739171672 1108 4 -0.3164691794776502 1108 5 -0.008881657667593856 1108 6 -0.26302555860900867 1108 7 -0.2602826303397876 1108 8 -0.4426368754313117 1108 9 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70-71 -2.5758336118881395 2316 72-73 -2.718440731228931 2316 74-75 -2.4454159131065722 2316 76-77 -1.8444367606162473 2316 78-79 -2.3558909412048266 2316 80-81 -2.7449007360603126 2316 82-83 -2.634832529607227 2316 84-85 -2.4952302677260683 2316 86-87 -2.1500764472586553 2316 88-89 -3.2205600736334823 2316 90-91 -2.9862032188327596 2316 92-93 -2.527383771719819 2316 94-95 -2.571064788496056 2316 96-97 -3.0763212192376166 2316 98-99 -3.495586343525634 2316 100-101 -3.9112593148665447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 9.0 6 48.0 7 104.0 8 169.0 9 303.0 10 514.0 11 791.0 12 1079.0 13 1512.0 14 1667.0 15 2128.0 16 2555.0 17 3326.0 18 3091.0 19 1837.0 20 1706.0 21 1732.0 22 1703.0 23 1758.0 24 1914.0 25 2064.0 26 1938.0 27 2087.0 28 2373.0 29 2762.0 30 3280.0 31 3791.0 32 4535.0 33 5634.0 34 7279.0 35 10049.0 36 13730.0 37 20443.0 38 16653.0 39 1674.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09237203237553 21.208675878957493 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19.395967837762903 44-45 17.96538339670469 28.55434093789607 33.36660329531052 20.11367237008872 46-47 19.37103929024081 27.764971482889734 31.66983523447402 21.194153992395435 48-49 19.34934490898433 27.898005418165113 31.523581692305253 21.229067980545302 50-51 18.748192947644 28.75203479046114 31.71461048862696 20.785161773267905 52-53 18.408691243801588 29.06798054530188 31.92004245813596 20.603285752760574 54-55 17.811425674498185 29.04540485733749 31.269704218881195 21.87346524928312 56-57 19.158045658338747 29.624847515090064 29.434736458547867 21.782370368023322 58-59 18.432851190570492 29.42721122922641 30.820962912501386 21.318974667701713 60-61 18.831366864303735 30.113314559792144 29.893101713778748 21.162216862125373 62-63 18.4075030496982 30.272809366138056 29.764658354588807 21.55502922957494 64-65 18.194816305191615 30.418957240854866 29.86129814166442 21.524928312289095 66-67 17.987096058713327 30.347630118702 29.476277422855578 22.18899639972909 68-69 18.02292422490138 30.315584353860046 29.129766638678095 22.531724782560477 70-71 18.2784583516514 29.861020345924278 28.434785708344716 23.4257355940796 72-73 18.48235927821169 29.25373489013165 28.532501069374693 23.731404762281965 74-75 18.493449089843317 29.24462540200567 28.390709906370304 23.87121560178071 76-77 18.827927702914323 28.730915765728266 28.73646610132534 23.704690430032073 78-79 18.395131190021473 28.42635370192803 29.074973651052773 24.103541456997725 80-81 18.148080670458324 28.957082428985597 28.573691798292167 24.321145102263905 82-83 18.505727095578333 28.56260198666054 28.27624720774386 24.655423710017267 84-85 18.67682704646631 28.01959728141189 28.119405586096548 25.184170086025254 86-87 19.064051914258968 27.840845986640645 28.10501848414172 24.99008361495867 88-89 19.139847329457627 27.68296026671647 27.75329521205787 25.42389719176803 90-91 18.549908455822486 27.280959208232225 28.25001687907828 25.919115456867004 92-93 18.822578856481993 27.703141585209362 27.829486224869694 25.644793333438955 94-95 18.775843221748705 27.38747801840909 27.88770773593574 25.948971023906463 96-97 18.95803298426831 26.893585336100507 27.694428161784508 26.453953517846674 98-99 19.30538172715895 26.869425389331603 27.302324107666227 26.52286877584322 100-101 19.366925166942593 26.647444174238167 28.080417613928915 25.90521304489033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 548.0 1 378.0 2 229.5 3 234.0 4 199.5 5 201.5 6 296.0 7 470.5 8 599.5 9 623.5 10 569.0 11 475.0 12 425.5 13 435.5 14 472.0 15 489.0 16 493.0 17 511.0 18 554.0 19 593.5 20 623.0 21 687.0 22 757.5 23 827.5 24 919.5 25 1073.0 26 1268.5 27 1446.0 28 1627.5 29 1744.5 30 1919.0 31 2181.5 32 2392.0 33 2622.5 34 2813.0 35 2951.5 36 3065.5 37 3130.0 38 3186.5 39 3121.0 40 3078.5 41 3178.5 42 3329.5 43 3634.5 44 4001.5 45 4388.0 46 4756.0 47 5156.0 48 5590.0 49 5390.5 50 4932.5 51 4676.0 52 4169.5 53 3521.0 54 2975.5 55 2600.0 56 2311.0 57 2052.5 58 1860.5 59 1670.0 60 1415.5 61 1141.5 62 887.5 63 655.5 64 475.5 65 361.0 66 262.0 67 200.0 68 158.5 69 112.5 70 87.0 71 61.5 72 46.5 73 50.0 74 35.0 75 29.5 76 28.5 77 21.5 78 18.5 79 10.5 80 6.5 81 4.0 82 3.0 83 4.5 84 3.5 85 2.0 86 2.0 87 1.0 88 0.0 89 1.5 90 2.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3707165602572836 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.9606470112957655E-4 16-17 0.0011881941033887295 18-19 7.921294022591531E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.9606470112957655E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002772452907907036 36-37 0.01148587633275772 38-39 0.01148587633275772 40-41 0.007129164620332378 42-43 0.005544905815814072 44-45 0.0015842588045183062 46-47 0.0015842588045183062 48-49 0.0 50-51 3.9606470112957655E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 3.9606470112957655E-4 62-63 0.0 64-65 0.0 66-67 0.0011881941033887295 68-69 0.0 70-71 3.9606470112957655E-4 72-73 0.0 74-75 0.0 76-77 0.09782798117900542 78-79 0.04039859951521681 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14892032762472077 88-89 0.3291297666386781 90-91 0.2744728378827965 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.921294022591531E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 126242.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.74124300945802 #Duplication Level Percentage of deduplicated Percentage of total 1 89.8529892192094 65.36018123920724 2 6.785364260045737 9.871516610953565 3 1.4243711205488403 3.1083157744649164 4 0.6272459980398563 1.8250661428050887 5 0.36044865512359797 1.3109741607388983 6 0.18621365566808235 0.812724766717891 7 0.15354459327017314 0.7818317200297841 8 0.11107481215289121 0.6463775922434689 9 0.07404987476859413 0.4847831941826017 >10 0.35282587389741915 4.517513981083949 >50 0.039202874877491016 1.9676494352117362 >100 0.026135249918327344 3.966984046513838 >500 0.003266906239790918 1.8551670600909362 >1k 0.003266906239790918 3.4909142757560874 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1485 1.1763121623548423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 1.1604695743096594 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1457 1.154132539091586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 972 0.7699497789958968 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 686 0.5434007699497789 RNA PCR Primer, Index 23 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 684 0.5418165111452606 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 491 0.3889355365092442 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 414 0.3279415725352894 RNA PCR Primer, Index 23 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 406 0.32160453731721617 RNA PCR Primer, Index 23 (96% over 25bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 373 0.2954642670426641 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 367 0.29071149062910917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 337 0.2669476085613346 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 214 0.16951569208345876 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.1671393038766813 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 180 0.14258329240664755 RNA PCR Primer, Index 23 (96% over 28bp) GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 164 0.12990922197050112 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 152 0.12040366914339126 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.11881941033887294 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 148 0.11723515153435465 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 144 0.11406663392531805 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 142 0.11248237512079973 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 139 0.11010598691402228 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 136 0.10772959870724481 No Hit TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC 129 0.10218469289143076 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.001584258804518306 0.0 20-21 0.0 0.0 0.0 0.004356711712425342 0.0 22-23 0.0 0.0 0.0 0.01703078214857179 0.0 24-25 0.0 0.0 0.0 0.03287337019375485 0.0 26-27 0.0 0.0 0.0 0.06376641688186183 0.0 28-29 0.0 0.0 0.0 0.1413950983032588 0.0 30-31 0.0 0.0 0.0 0.2697200614692416 0.0 32-33 0.0 0.0 0.0 0.5093392056526355 0.0 34-35 0.0 0.0 0.0 0.9073842302878599 0.0 36-37 0.0 0.0 0.0 1.4206840829517908 0.0 38-39 0.0 0.0 0.0 1.9498265236609051 0.0 40-41 0.0 0.0 0.0 2.389062277213606 0.0 42-43 0.0 0.0 0.0 2.819584607341455 0.0 44-45 0.0 0.0 0.0 3.346350659843792 0.0 46-47 0.0 0.0 0.0 3.97490534053643 0.0 48-49 0.0 0.0 0.0 4.546822768967539 0.0 50-51 0.0 0.0 0.0 5.0834904389981155 0.0 52-53 0.0 0.0 0.0 5.615405332615136 0.0 54-55 0.0 0.0 0.0 6.2364347839863115 0.0 56-57 0.0 0.0 0.0 6.953311893030845 0.0 58-59 0.0 0.0 0.0 7.659891319846009 0.0 60-61 0.0 0.0 0.0 8.366470746661175 0.0 62-63 0.0 0.0 0.0 9.076218691085376 0.0 64-65 0.0 0.0 0.0 9.809730517577352 0.0 66-67 0.0 0.0 0.0 10.639882131144944 0.0 68-69 0.0 0.0 0.0 11.520333961755993 0.0 70-71 0.0 0.0 0.0 12.454650591720664 0.0 72-73 0.0 0.0 0.0 13.388967221685334 0.0 74-75 0.0 0.0 0.0 14.320511398742099 0.0 76-77 0.0 0.0 0.0 15.29166204591182 0.0 78-79 0.0 0.0 0.0 16.3693540976854 0.0 80-81 0.0 0.0 0.0 17.426450785000235 0.0 82-83 0.0 0.0 0.0 18.438396096386306 0.0 84-85 0.0 0.0 0.0 19.545396936043474 0.0 86-87 0.0 0.0 0.0 20.70705470445652 0.0 88-89 0.0 0.0 0.0 21.774449074000728 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATGTC 15 6.6424004E-4 94.86212 4 GGTATAG 25 0.0049456093 57.189175 1 CTTGGAC 55 4.980633E-5 43.11915 3 TGGACTG 45 9.661297E-4 42.160942 5 ATGGGTA 55 0.002589346 34.49532 5 TTGGACT 60 0.00396585 31.620707 4 AGAGTGA 60 0.00396585 31.620707 6 TACCTGG 65 0.005866121 29.188347 2 GGTATCA 2235 0.0 26.440928 1 TACACAG 90 8.9791237E-4 26.35059 5 GAGTACT 1400 0.0 25.927961 12-13 CATGGGT 100 0.0016594989 23.715532 4 GTACTTT 1580 0.0 22.974142 14-15 ACTTTTT 1655 0.0 21.789665 16-17 GTATCAA 2860 0.0 20.73036 2 ATCAACG 3085 0.0 19.064672 4 TATCAAC 3095 0.0 19.003077 3 CAACGCA 3115 0.0 18.881065 6 AACGCAG 3095 0.0 18.849825 7 TCAACGC 3145 0.0 18.700958 5 >>END_MODULE