FastQCFastQC Report
Thu 26 May 2016
SRR938858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126829
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15241.20161792649946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15201.198464073674002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.7411554139826064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.5661165821696931No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.44232785877047043No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.2893659967357623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3130.24678898359208065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.23732742511570698No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2280.17976961105110031No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.1545387884474371No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1780.14034645073287655No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1760.1387695243201476No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1590.12536564981195153No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.12378872339922257No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1480.1166925545419423No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.11432716492284889No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGA250.001615991637.9877242-43
GTAGCCC702.0445306E-433.9243243
ACTTTAC600.003940166431.6627048
CAGACCT650.005828229.227114
TACCTGG908.909811E-426.3855882
GAGTACT13000.023.93918212-13
TAGCCCT1000.001646728523.7470284
GGTATCA23650.023.3229051
ATGGGAT1252.2423867E-422.797155
GTACTTT14400.021.44678714-15
GTATCAA27800.019.8176632
TGTAGCC1200.004743899719.789192
ACTTTTT16000.019.14983616-17
TACAGAG1250.0060064318.9976232
AGCCCTG1559.568035E-418.3847985
TATCAAC30000.018.3643683
ATCAACG30600.018.3147034
TCAACGC30900.018.1368895
AACGCAG31450.017.819717
CAACGCA31650.017.7071046
Produced by FastQC (version 0.11.5)