##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938858_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 126829 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.925182726348076 31.0 31.0 34.0 28.0 34.0 2 30.92408676249123 33.0 31.0 34.0 27.0 34.0 3 30.86261028629099 33.0 31.0 34.0 27.0 34.0 4 34.41481049286835 37.0 35.0 37.0 32.0 37.0 5 34.43493207389477 37.0 35.0 37.0 32.0 37.0 6 34.509165884773985 37.0 35.0 37.0 32.0 37.0 7 34.44455920964448 37.0 35.0 37.0 31.0 37.0 8 34.42437455156155 37.0 35.0 37.0 31.0 37.0 9 35.878016857343354 39.0 37.0 39.0 30.0 39.0 10-11 35.78521079563822 39.0 37.0 39.0 30.0 39.0 12-13 35.66204101585599 39.0 36.0 39.0 30.0 39.0 14-15 36.63530817084421 40.0 36.5 41.0 30.0 41.0 16-17 36.49325469726955 40.0 36.5 41.0 30.0 41.0 18-19 36.54275441736512 40.0 36.0 41.0 30.0 41.0 20-21 36.48407698554747 40.0 36.0 41.0 30.0 41.0 22-23 36.37230049909721 40.0 36.0 41.0 30.0 41.0 24-25 36.24606359744222 40.0 36.0 41.0 29.5 41.0 26-27 35.98596535492671 39.0 35.0 41.0 28.0 41.0 28-29 35.72106537148444 39.0 35.0 41.0 27.0 41.0 30-31 35.32704271105189 38.5 35.0 41.0 26.5 41.0 32-33 34.91837040424509 38.0 35.0 40.0 25.0 41.0 34-35 34.46986493625275 38.0 35.0 40.0 23.5 41.0 36-37 34.08333661859669 38.0 34.0 40.0 20.5 41.0 38-39 33.75776439142467 38.0 34.0 40.0 17.0 41.0 40-41 33.38621293237351 38.0 33.0 40.0 12.0 41.0 42-43 33.06444109785617 38.0 33.0 40.0 5.0 41.0 44-45 32.572483422561085 38.0 33.0 40.0 2.0 41.0 46-47 31.83020839082544 38.0 31.5 40.0 2.0 41.0 48-49 31.190516364553847 38.0 30.5 40.0 2.0 41.0 50-51 29.930500910675 36.0 28.5 39.0 2.0 40.5 52-53 29.8814663838712 36.5 28.0 39.5 2.0 40.0 54-55 30.23608165324965 37.0 29.0 40.0 2.0 41.0 56-57 30.08939201602157 37.0 28.0 40.0 2.0 41.0 58-59 29.834943112379662 36.0 28.0 40.0 2.0 41.0 60-61 29.479354090941346 36.0 27.0 40.0 2.0 41.0 62-63 29.027099480402747 35.0 26.0 39.0 2.0 41.0 64-65 28.584854410268946 35.0 25.5 39.0 2.0 41.0 66-67 28.12667844105055 35.0 23.0 39.0 2.0 40.5 68-69 27.655153789748404 35.0 21.5 38.0 2.0 40.0 70-71 27.156931774278753 35.0 19.5 37.0 2.0 40.0 72-73 26.655484944295075 34.0 15.5 37.0 2.0 39.0 74-75 26.12330776084334 34.0 7.5 36.0 2.0 39.0 76-77 25.628318444519785 34.0 2.0 36.0 2.0 38.5 78-79 25.09509654731962 33.5 2.0 35.0 2.0 37.0 80-81 24.55909925963305 33.0 2.0 35.0 2.0 37.0 82-83 24.081657191967135 33.0 2.0 35.0 2.0 36.0 84-85 23.640835297920823 32.5 2.0 35.0 2.0 36.0 86-87 23.12659959472991 32.0 2.0 35.0 2.0 35.5 88-89 22.61774515292244 31.5 2.0 35.0 2.0 35.0 90-91 22.04982299001017 31.0 2.0 35.0 2.0 35.0 92-93 21.528983907465957 31.0 2.0 35.0 2.0 35.0 94-95 20.98405333165128 30.5 2.0 34.5 2.0 35.0 96-97 20.338447831331948 29.5 2.0 34.0 2.0 35.0 98-99 19.62833027146788 29.0 2.0 34.0 2.0 35.0 100-101 17.740236854347195 23.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.9109803573269986 1101 2 -0.6937117505075641 1101 3 -0.9380661411038744 1101 4 -0.6701906260935075 1101 5 -0.656524739942796 1101 6 -0.4223512312094684 1101 7 -1.0028250779409191 1101 8 -0.9585158837160108 1101 9 -0.6855527528523382 1101 10-11 -0.49337658279236507 1101 12-13 -1.1316870968707846 1101 14-15 -0.3756821056898403 1101 16-17 -0.09535794741201897 1101 18-19 0.2076900837813156 1101 20-21 0.1560352199280004 1101 22-23 -0.09977559554001658 1101 24-25 0.041514372201135075 1101 26-27 0.2721521430966547 1101 28-29 0.4733661401614455 1101 30-31 -0.21216593260950845 1101 32-33 -0.269666329973127 1101 34-35 -0.2650864903765111 1101 36-37 0.32748910461062763 1101 38-39 0.7879273291922075 1101 40-41 1.076136030524701 1101 42-43 0.5460043346647296 1101 44-45 0.8008236434620741 1101 46-47 0.252830116843036 1101 48-49 -0.408356832789746 1101 50-51 -0.286943005199241 1101 52-53 -0.9427455008265291 1101 54-55 -1.1481824455371523 1101 56-57 -1.6209948935750411 1101 58-59 -1.3768217674998446 1101 60-61 -1.1677316208157755 1101 62-63 -1.3456614492468582 1101 64-65 -0.9782140953446863 1101 66-67 -1.6114671166618635 1101 68-69 -1.2491427016870489 1101 70-71 -1.5423525882051763 1101 72-73 -1.1516511693609992 1101 74-75 -1.700702557020822 1101 76-77 -1.318478264875548 1101 78-79 -1.404356256080451 1101 80-81 -1.0496115435721443 1101 82-83 -1.5039718474137196 1101 84-85 -1.7580003322925464 1101 86-87 -1.4964139881109624 1101 88-89 -1.8699564428023088 1101 90-91 -1.9591221696560197 1101 92-93 -1.4475316170555068 1101 94-95 -1.0326730311559515 1101 96-97 -0.6235750998260947 1101 98-99 -0.765725947431438 1101 100-101 -0.6621414671590848 1102 1 -0.765238352296457 1102 2 -1.1185770038313159 1102 3 -0.918950079300064 1102 4 -0.7242330263809649 1102 5 -0.739672422299968 1102 6 -0.5688837500740007 1102 7 -1.024384546140702 1102 8 -0.9496046368601014 1102 9 -0.9360019084398346 1102 10-11 -1.3357768702519408 1102 12-13 -1.07272195134437 1102 14-15 -0.6680644269259162 1102 16-17 -1.2658933408723172 1102 18-19 -1.2645700671349616 1102 20-21 -1.0861854052965754 1102 22-23 -1.4898223077211128 1102 24-25 -1.0233796270802173 1102 26-27 -0.7253325856133657 1102 28-29 -0.9425878663063969 1102 30-31 -1.009399134190545 1102 32-33 -0.4190015797036395 1102 34-35 -0.27281196648143435 1102 36-37 -0.7160250887059405 1102 38-39 -0.5954718802939496 1102 40-41 -0.7581435239129561 1102 42-43 -0.4967912097118372 1102 44-45 -1.0939302192759826 1102 46-47 -0.46851375667474926 1102 48-49 -0.16157278679621712 1102 50-51 -0.19006912808820786 1102 52-53 -0.8650954828603048 1102 54-55 -0.5756707674918751 1102 56-57 -0.4232586377360228 1102 58-59 -0.6923805170506867 1102 60-61 -0.9872429395365785 1102 62-63 -0.185043411158464 1102 64-65 -0.7030074837744387 1102 66-67 -1.0548735126374282 1102 68-69 -1.3689774124308514 1102 70-71 -1.6964668533866387 1102 72-73 -2.3727794481967877 1102 74-75 -2.2300234337725335 1102 76-77 -1.7067283547066658 1102 78-79 -1.777874042641713 1102 80-81 -1.9921555608197217 1102 82-83 -1.7922938021388362 1102 84-85 -1.9683488770284399 1102 86-87 -2.0857420513520673 1102 88-89 -2.0984149408260215 1102 90-91 -1.9479831110861312 1102 92-93 -1.6843264427831386 1102 94-95 -1.6833737138724452 1102 96-97 -2.0553753154207755 1102 98-99 -1.9969512438741255 1102 100-101 -1.4857131523908507 1103 1 -0.5670880177824849 1103 2 -0.007859438568296184 1103 3 0.5029966608284937 1103 4 0.04947811096653254 1103 5 0.1984096976003329 1103 6 0.4241636066097172 1103 7 0.38778913876025456 1103 8 0.6815117215289845 1103 9 0.28753213327602367 1103 10-11 0.5975826994712605 1103 12-13 0.8034750839435745 1103 14-15 0.4700045747794519 1103 16-17 0.3782100305037801 1103 18-19 0.25917386569987855 1103 20-21 0.9736335636132978 1103 22-23 0.8914252326173298 1103 24-25 0.9074218946304029 1103 26-27 0.7474799553809888 1103 28-29 1.4222619303615858 1103 30-31 1.0555359307445258 1103 32-33 0.8377870861759362 1103 34-35 0.3410211700789745 1103 36-37 0.11265128542498104 1103 38-39 -0.3368138716359468 1103 40-41 -0.2802476823807538 1103 42-43 0.2901037135467277 1103 44-45 0.5859858242764275 1103 46-47 0.18191914375815088 1103 48-49 -1.419640476682087 1103 50-51 -0.9761769596505836 1103 52-53 -0.952200297237848 1103 54-55 -1.3128477319415701 1103 56-57 -1.2166470674880863 1103 58-59 -1.454875597727586 1103 60-61 -1.9564479769234353 1103 62-63 -1.7890706970039325 1103 64-65 -1.3567855239161197 1103 66-67 -1.4106389593119673 1103 68-69 -1.4753331778775234 1103 70-71 -1.626514078888409 1103 72-73 -1.634880983025596 1103 74-75 -1.4705783334183415 1103 76-77 -1.1918392034538776 1103 78-79 -0.7385522533199271 1103 80-81 -1.1766301771125178 1103 82-83 -0.9811630137353191 1103 84-85 -1.2182832860537616 1103 86-87 -0.9089743745843926 1103 88-89 -1.1359329783440586 1103 90-91 -0.7701987742108827 1103 92-93 -1.6435288565310096 1103 94-95 -1.5122796564147514 1103 96-97 -1.8315461143188472 1103 98-99 -1.607340854263736 1103 100-101 -1.4533768018726825 1104 1 0.3102013595068911 1104 2 -0.3472977780066344 1104 3 -0.1508494930176596 1104 4 -0.04209698060855516 1104 5 -0.3803448811542438 1104 6 -0.2278510454049325 1104 7 0.4854692364403519 1104 8 -0.14632710630984036 1104 9 0.19962004536393607 1104 10-11 -0.26810716621860564 1104 12-13 0.15787068833918028 1104 14-15 0.19588980066468054 1104 16-17 0.7732039254976755 1104 18-19 0.7457428522688687 1104 20-21 0.670825260804996 1104 22-23 0.5962481874402883 1104 24-25 0.2370922243007243 1104 26-27 0.7288596867607211 1104 28-29 1.106327864427513 1104 30-31 1.050041425250015 1104 32-33 1.1760044243932768 1104 34-35 1.1239882030460038 1104 36-37 0.7597819325556259 1104 38-39 1.2355304507083744 1104 40-41 0.871156469023397 1104 42-43 1.6039010273440368 1104 44-45 1.1561933944839993 1104 46-47 1.276241488080501 1104 48-49 1.5257197186781077 1104 50-51 1.102577619103993 1104 52-53 1.159521314483765 1104 54-55 0.6431962241023861 1104 56-57 0.9970281461871267 1104 58-59 1.2722306293786403 1104 60-61 0.9486191781437228 1104 62-63 1.0302211222878874 1104 64-65 0.49699957986898724 1104 66-67 0.5704355217625121 1104 68-69 1.0029940504497041 1104 70-71 0.9116422092678782 1104 72-73 0.9273900792454661 1104 74-75 0.8917537789137704 1104 76-77 0.969332957718283 1104 78-79 0.49679573202805827 1104 80-81 0.8035285530462097 1104 82-83 0.9507661681938622 1104 84-85 0.9187741010036277 1104 86-87 1.1807692151591986 1104 88-89 1.1552758128647334 1104 90-91 1.2014129474008328 1104 92-93 1.0847983717962215 1104 94-95 1.2914443973093022 1104 96-97 0.7346932019204679 1104 98-99 0.7357604888376095 1104 100-101 1.0496757011798223 1105 1 -0.6647633083711604 1105 2 -0.07022664266778378 1105 3 0.011745567215193375 1105 4 -0.025137562074334596 1105 5 -0.24803449687535561 1105 6 0.3377994021352748 1105 7 0.38128995115870623 1105 8 0.436417358324384 1105 9 0.40414255697306345 1105 10-11 0.6770102710248196 1105 12-13 0.9294905820063164 1105 14-15 0.7837153609311827 1105 16-17 0.31590531859277604 1105 18-19 0.2605486938463599 1105 20-21 0.4582604852480969 1105 22-23 0.0546235828235595 1105 24-25 0.598960452310692 1105 26-27 0.7100158883893641 1105 28-29 0.6532018128762687 1105 30-31 0.7057046596534065 1105 32-33 0.8355373673907849 1105 34-35 0.015468114210953843 1105 36-37 0.29575339766095254 1105 38-39 0.5652296229272338 1105 40-41 0.12420176143877626 1105 42-43 0.2872915650652885 1105 44-45 0.6470406924179102 1105 46-47 0.2995606885650517 1105 48-49 -0.6940124301878967 1105 50-51 -0.7125849086569609 1105 52-53 -0.49556987604049496 1105 54-55 -0.6084357834351302 1105 56-57 -0.7087730517400566 1105 58-59 -0.033240802077042275 1105 60-61 0.022648386031193013 1105 62-63 -0.008105817613856203 1105 64-65 -0.43018259928169655 1105 66-67 -0.09417851686727374 1105 68-69 0.3532195030373586 1105 70-71 0.44350841829944443 1105 72-73 0.017537464668443192 1105 74-75 -0.020353361539822146 1105 76-77 0.46139164269035504 1105 78-79 0.13193268606265107 1105 80-81 0.4338872582080633 1105 82-83 -0.4588845485434696 1105 84-85 -0.9934676380097649 1105 86-87 -0.9855273054680289 1105 88-89 -0.7619797225010423 1105 90-91 -0.3788414438771781 1105 92-93 -1.1704004975758764 1105 94-95 -0.8749530722377763 1105 96-97 -0.7416511011954867 1105 98-99 -0.6079032839600238 1105 100-101 0.42601327436778647 1106 1 0.9913853715732586 1106 2 0.7479762290320764 1106 3 0.7979313013513689 1106 4 1.161522928893703 1106 5 0.9558793521288109 1106 6 1.0956155207086908 1106 7 0.885921725128135 1106 8 0.826586418074271 1106 9 1.501467707505718 1106 10-11 1.3613548750081392 1106 12-13 1.3699433379118275 1106 14-15 1.5649508866375257 1106 16-17 1.774335147217137 1106 18-19 1.441129981479527 1106 20-21 1.6388996700558778 1106 22-23 1.5771745323372244 1106 24-25 1.5600246023801674 1106 26-27 1.3902787415621276 1106 28-29 1.1780975979619512 1106 30-31 1.0166703845260372 1106 32-33 1.6881022122263616 1106 34-35 1.5729889571938216 1106 36-37 2.043090127628524 1106 38-39 1.9065334672996315 1106 40-41 1.9157140356397875 1106 42-43 1.7086961505215115 1106 44-45 1.8252481958917386 1106 46-47 2.4018070989402247 1106 48-49 2.271886133962166 1106 50-51 2.033101751834007 1106 52-53 1.3008926065903523 1106 54-55 1.5996756107288341 1106 56-57 1.8348235207472037 1106 58-59 1.7581846263620484 1106 60-61 1.704966588902895 1106 62-63 1.9044983973004577 1106 64-65 2.082815316420014 1106 66-67 2.2283666429289255 1106 68-69 1.839092592430596 1106 70-71 1.8067842404393204 1106 72-73 2.2369677564702037 1106 74-75 2.285198977506031 1106 76-77 2.0883311980400556 1106 78-79 2.140240930620319 1106 80-81 1.9767311674252959 1106 82-83 1.833496183276818 1106 84-85 1.9840393096511875 1106 86-87 2.104097520840444 1106 88-89 2.5142187490135477 1106 90-91 2.2336527664053634 1106 92-93 1.8996550837117532 1106 94-95 1.6451082987673225 1106 96-97 1.8939440817595887 1106 98-99 1.7403482213665207 1106 100-101 1.8946040571375882 1107 1 0.2151419473743097 1107 2 0.2910178359187334 1107 3 0.24418907275261148 1107 4 0.573287634776058 1107 5 0.12601325935023766 1107 6 0.12257630502241312 1107 7 0.21279881742847095 1107 8 0.35940564332534564 1107 9 0.4791697639380459 1107 10-11 0.6442338802687857 1107 12-13 0.7139474029524777 1107 14-15 0.921030513610269 1107 16-17 0.9004709673988671 1107 18-19 0.37686647119735994 1107 20-21 0.24889488765511203 1107 22-23 0.49159944864521066 1107 24-25 0.3344447362873879 1107 26-27 0.3147466994770056 1107 28-29 0.759757091027474 1107 30-31 1.1391596241243178 1107 32-33 0.7553248329819766 1107 34-35 0.8494416114262506 1107 36-37 0.5617414906127465 1107 38-39 0.9340920517090012 1107 40-41 0.5035308696904863 1107 42-43 0.6646681734770397 1107 44-45 0.860279281496716 1107 46-47 1.1874359846408424 1107 48-49 1.210731184177373 1107 50-51 0.4512330287350146 1107 52-53 0.9097818940126352 1107 54-55 1.6393396470750794 1107 56-57 1.3971740707232954 1107 58-59 1.194369466151624 1107 60-61 1.555456784981324 1107 62-63 1.7715177660235533 1107 64-65 1.78926557945206 1107 66-67 1.3652030848227596 1107 68-69 1.9256540462804317 1107 70-71 2.213497535513447 1107 72-73 1.7222618741172617 1107 74-75 1.5088061032831703 1107 76-77 1.524575817839196 1107 78-79 2.1423033409503027 1107 80-81 1.1395719134798163 1107 82-83 1.6612101957110603 1107 84-85 1.934930031793705 1107 86-87 1.4094538096974496 1107 88-89 1.6488134405487003 1107 90-91 1.8915317716660027 1107 92-93 2.2811711047543177 1107 94-95 2.4485217373134702 1107 96-97 2.5932506336486334 1107 98-99 2.5895232572345215 1107 100-101 2.631601905057245 1108 1 0.2614834107889443 1108 2 0.08817230746344862 1108 3 -0.0275588947270613 1108 4 -0.9350456985572748 1108 5 -3.00081236304095E-6 1108 6 -0.10242369497758119 1108 7 -0.6209410199698979 1108 8 -0.13713907212181198 1108 9 -0.05294405720016471 1108 10-11 -0.6567823798938193 1108 12-13 -0.9217233287548439 1108 14-15 -0.5720589172840391 1108 16-17 -0.38448838219463255 1108 18-19 -0.30788962636361106 1108 20-21 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-1.403909388434716 2316 94-95 -0.8963067771968412 2316 96-97 -0.8089191827515201 2316 98-99 -1.6853919422485966 2316 100-101 -2.390042417346244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2616.0 3 481.0 4 364.0 5 352.0 6 390.0 7 439.0 8 582.0 9 593.0 10 677.0 11 934.0 12 1228.0 13 1681.0 14 1832.0 15 2265.0 16 2608.0 17 3101.0 18 2844.0 19 1607.0 20 1588.0 21 1494.0 22 1506.0 23 1627.0 24 1769.0 25 2008.0 26 2079.0 27 2443.0 28 2603.0 29 2995.0 30 3473.0 31 4078.0 32 4608.0 33 5464.0 34 7074.0 35 9718.0 36 12546.0 37 17473.0 38 15273.0 39 2415.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.10184767913474 19.482460093451294 15.696971134460757 18.718721092953203 2 16.583216186760595 22.99758335834215 38.74840075184407 21.67079970305318 3 20.469430158241096 27.660196399086733 32.469050948419564 19.4013224942526 4 13.06665613647133 19.198388880113725 37.43247512241352 30.30247986100142 5 12.122409623318529 38.479047784645054 33.531448081817175 15.867094510219243 6 26.430252950563254 36.19357590886828 19.260423743541942 18.115747397026528 7 23.053454421865492 37.37067159873221 22.271753649649458 17.30412032975284 8 24.25897529127211 32.30867730053591 21.52884265772958 21.9035047504624 9 25.62995017254041 18.18662792074986 23.28624335699677 32.897178549712955 10-11 23.439111009279415 27.532086860682387 27.00313264149229 22.02566948854591 12-13 26.69908857818988 26.6113834885292 27.843995559436003 18.845532373844918 14-15 23.348261182064466 27.332180176764687 29.628620398233934 19.690938242936916 16-17 18.699791258144096 30.226057941678793 30.760563603010944 20.31358719716617 18-19 17.946742218586877 28.239901055435034 35.013099090768996 18.800257635209093 20-21 20.157696804980567 26.76934867111209 34.33184274563095 18.741111778276395 22-23 19.66376724536534 26.961851730365677 34.05285351269962 19.321527511569364 24-25 19.4325775868542 27.113606993388373 34.8835554993499 18.570259920407526 26-27 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31.577010002018614 20.282288867163537 52-53 18.892367611727035 28.782451404528846 31.58675939156214 20.73842159218198 54-55 18.35276702525984 28.76700942791327 31.63654642317161 21.243677123655274 56-57 19.029020948582193 29.153509500858675 30.413431784546958 21.40403776601217 58-59 19.091434132139213 28.249879348402256 30.711963100390037 21.94672341906849 60-61 19.037826508215847 27.77450418207344 31.548059241097025 21.63961006861369 62-63 19.580342090057858 27.533817208982043 31.269738248074656 21.616102452885443 64-65 19.19739352815892 27.99146483400765 31.407114749685277 21.404026888148152 66-67 18.8979682328535 28.076155636847353 30.910415379986382 22.115460750312764 68-69 18.848262892210236 28.340888844897556 30.76789072552707 22.04295753736514 70-71 19.310533234087707 28.077538286112475 30.09161743977955 22.52031104002027 72-73 19.341570302572038 27.447634807463157 30.22553354895666 22.98526134100815 74-75 19.34491645190789 28.000585885696864 30.001702235470628 22.652795426924616 76-77 19.9069034717247 27.43774763399448 29.95950776002312 22.6958411342577 78-79 19.719446807668717 27.48234176839523 30.173119917693846 22.62509150624221 80-81 19.769287085221315 27.667346010012068 29.764736144363756 22.798630760402858 82-83 19.929156607432617 27.274706138441445 29.567815728024698 23.22832152610124 84-85 20.29865395955942 26.544028306031258 29.58835146655479 23.568966267854528 86-87 20.942068561568476 26.230221863399265 29.422031201766675 23.40567837326558 88-89 21.45017018918705 25.977994142325656 29.220296050027706 23.35153961845959 90-91 21.35649048073383 25.80119005673158 29.51982042257703 23.322499039957563 92-93 21.613254419129436 26.016350284051544 29.031057621884838 23.339337674934182 94-95 21.47551628024832 25.63782782212087 28.933073609527433 23.953582288103384 96-97 21.91850244682704 24.98693441870041 28.748634052864137 24.345929081608414 98-99 22.495140134373802 24.9426911976055 28.41724767300528 24.144920995015422 100-101 23.713082506787835 24.02457076364097 28.962075216300292 23.300271513270904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 718.0 1 486.5 2 257.5 3 262.5 4 227.0 5 222.5 6 309.0 7 472.0 8 636.0 9 723.5 10 637.0 11 501.0 12 469.0 13 459.0 14 512.0 15 546.5 16 547.5 17 572.0 18 579.0 19 596.0 20 659.0 21 735.5 22 805.5 23 890.0 24 999.0 25 1139.5 26 1352.5 27 1527.0 28 1650.0 29 1813.0 30 1977.0 31 2188.5 32 2398.5 33 2627.0 34 2819.5 35 2879.5 36 3034.5 37 3191.0 38 3130.5 39 3052.5 40 3041.0 41 3125.5 42 3222.0 43 3414.0 44 3777.0 45 4080.5 46 4392.0 47 4790.0 48 5208.5 49 5167.0 50 4654.0 51 4101.0 52 3682.5 53 3443.0 54 3167.5 55 2873.0 56 2537.5 57 2250.5 58 2052.5 59 1775.0 60 1534.5 61 1302.0 62 1061.5 63 849.0 64 616.0 65 468.0 66 394.0 67 317.5 68 237.5 69 183.0 70 148.5 71 122.0 72 105.5 73 92.0 74 75.5 75 58.0 76 55.0 77 39.5 78 30.5 79 30.5 80 25.5 81 22.0 82 17.5 83 12.5 84 7.5 85 4.5 86 4.0 87 4.0 88 5.0 89 2.5 90 1.0 91 2.0 92 1.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27280826940210834 2 0.16321188371744633 3 0.19711580159111874 4 0.16478881013017527 5 0.23890435152843595 6 0.19159655914656742 7 0.24442359397298724 8 0.24836591000480962 9 0.1513849356219792 10-11 0.20381773884521678 12-13 0.21170237090886154 14-15 0.1734619054001845 16-17 0.2814813646721176 18-19 0.23141395106797338 20-21 0.2026350440356701 22-23 0.24402936236980502 24-25 0.24402936236980502 26-27 0.348500737213098 28-29 0.3205102933871591 30-31 0.29094292314849124 32-33 0.19159655914656742 34-35 0.3599334537053829 36-37 0.3981739192140598 38-39 0.37846233905494797 40-41 0.3354910943080841 42-43 0.37373155981676115 44-45 0.3843758131026816 46-47 0.3891065923408684 48-49 0.40605855127770457 50-51 0.3977796876108776 52-53 0.3985681508172421 54-55 0.39580852959496643 56-57 0.3721546334040322 58-59 0.34061610514945323 60-61 0.3126256613235143 62-63 0.3847700447058638 64-65 0.41630857296044277 66-67 0.4218278154049941 68-69 0.4269528262463632 70-71 0.42813552105590996 72-73 0.4167028045636251 74-75 0.41354895173816714 76-77 0.40093354043633556 78-79 0.37176040180084996 80-81 0.3796450338644947 82-83 0.38950082394405067 84-85 0.39068351875359736 86-87 0.38634697111859273 88-89 0.393443139975873 90-91 0.41985665738908295 92-93 0.41906819418271846 94-95 0.4265585946431809 96-97 0.42813552105590996 98-99 0.4253758998336342 100-101 0.3942316031822375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 126829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.95716279399821 #Duplication Level Percentage of deduplicated Percentage of total 1 90.93787139871317 69.98320573370444 2 6.092987992295398 9.377981376499065 3 1.3237162411376582 3.0560833878687053 4 0.5471087250522519 1.6841574087945188 5 0.27662800704889146 1.064425328592041 6 0.1997868939797549 0.9225019514464358 7 0.11679849186508749 0.629193638678851 8 0.08401295028892258 0.5172318633750955 9 0.07376746854637105 0.5109241577241799 >10 0.2909716814884636 3.8618927847731985 >50 0.03483463792467522 1.9285810027675057 >100 0.016392770788082454 2.314139510679734 >500 0.00307364452276546 1.74959985492277 >1k 0.002049096348510307 2.400082000173462 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1524 1.20161792649946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1520 1.198464073674002 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 940 0.7411554139826064 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 718 0.5661165821696931 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.44232785877047043 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.2893659967357623 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 313 0.24678898359208065 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.23732742511570698 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 228 0.17976961105110031 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 196 0.1545387884474371 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 178 0.14034645073287655 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 176 0.1387695243201476 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 159 0.12536564981195153 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.12378872339922257 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 148 0.1166925545419423 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.11432716492284889 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.942316031822375E-4 0.0 18-19 0.0 0.0 0.0 7.88463206364475E-4 0.0 20-21 0.0 0.0 0.0 0.0035480844286401377 0.0 22-23 0.0 0.0 0.0 0.010644253285920412 0.0 24-25 0.0 0.0 0.0 0.02010581176229411 0.0 26-27 0.0 0.0 0.0 0.04612509757232179 0.0 28-29 0.0 0.0 0.0 0.10959638568466201 0.0 30-31 0.0 0.0 0.0 0.2448178255761695 0.0 32-33 0.0 0.0 0.0 0.4856933351205166 0.0 34-35 0.0 0.0 0.0 0.8511460312704507 0.0 36-37 0.0 0.0 0.0 1.3403874508196074 0.0 38-39 0.0 0.0 0.0 1.7980903421141852 0.0 40-41 0.0 0.0 0.0 2.2151873782809925 0.0 42-43 0.0 0.0 0.0 2.647659446971907 0.0 44-45 0.0 0.0 0.0 3.2027375442524972 0.0 46-47 0.0 0.0 0.0 3.8303542565186195 0.0 48-49 0.0 0.0 0.0 4.419730503276065 0.0 50-51 0.0 0.0 0.0 4.948000851540263 0.0 52-53 0.0 0.0 0.0 5.442761513533971 0.0 54-55 0.0 0.0 0.0 6.025041591434135 0.0 56-57 0.0 0.0 0.0 6.688533379589842 0.0 58-59 0.0 0.0 0.0 7.376073295539664 0.0 60-61 0.0 0.0 0.0 8.123536415173186 0.0 62-63 0.0 0.0 0.0 8.815412878758012 0.0 64-65 0.0 0.0 0.0 9.52502976448604 0.0 66-67 0.0 0.0 0.0 10.339512256660543 0.0 68-69 0.0 0.0 0.0 11.224956437407847 0.0 70-71 0.0 0.0 0.0 12.101333291281962 0.0 72-73 0.0 0.0 0.0 12.998604420124735 0.0 74-75 0.0 0.0 0.0 13.906125570650246 0.0 76-77 0.0 0.0 0.0 14.842425628208058 0.0 78-79 0.0 0.0 0.0 15.883197060609167 0.0 80-81 0.0 0.0 0.0 16.970487822185778 0.0 82-83 0.0 0.0 0.0 17.975778410300485 0.0 84-85 0.0 0.0 0.0 19.065434561496186 0.0 86-87 0.0 0.0 0.0 20.200427347057847 0.0 88-89 0.0 0.0 0.0 21.336602827429058 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATCGA 25 0.0016159916 37.98772 42-43 GTAGCCC 70 2.0445306E-4 33.924324 3 ACTTTAC 60 0.0039401664 31.662704 8 CAGACCT 65 0.0058282 29.22711 4 TACCTGG 90 8.909811E-4 26.385588 2 GAGTACT 1300 0.0 23.939182 12-13 TAGCCCT 100 0.0016467285 23.747028 4 GGTATCA 2365 0.0 23.322905 1 ATGGGAT 125 2.2423867E-4 22.79715 5 GTACTTT 1440 0.0 21.446787 14-15 GTATCAA 2780 0.0 19.817663 2 TGTAGCC 120 0.0047438997 19.78919 2 ACTTTTT 1600 0.0 19.149836 16-17 TACAGAG 125 0.00600643 18.997623 2 AGCCCTG 155 9.568035E-4 18.384798 5 TATCAAC 3000 0.0 18.364368 3 ATCAACG 3060 0.0 18.314703 4 TCAACGC 3090 0.0 18.136889 5 AACGCAG 3145 0.0 17.81971 7 CAACGCA 3165 0.0 17.707104 6 >>END_MODULE