FastQCFastQC Report
Thu 26 May 2016
SRR938858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126829
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15451.2181756538331139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14421.136963943577573No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT13621.073886887068415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.738790024363513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.5440396123914878No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT6660.5251164954387403RNA PCR Primer, Index 23 (95% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.36742385416584533No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG3800.2996160184185005RNA PCR Primer, Index 23 (96% over 30bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3640.2870006071166689No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.28305829108484654No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT3470.27359673260847284RNA PCR Primer, Index 23 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.26413517413209914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.16321188371744633No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1800.14192337714560552No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1650.13009642905013838No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1640.12930796584377388No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1610.12694257622468047No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1570.12378872339922257No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.12221179698649362No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT1500.11826948095467124RNA PCR Primer, Index 23 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.11117331209739097No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC1390.10959638568466203No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1370.10801945927193307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG200.002075032671.204722
TACCGCA250.00503934456.918857
TTATTAC405.386794E-447.4698143
TGGGAGT459.6601556E-442.162116
GAGTGCA500.001621709837.94597
ACATGGA651.326477E-436.5152363
TAAGTAC550.00253439134.6465531
CCCTAGA550.002579035734.52352
ATGGGGG1102.7216756E-630.2080635
CCTGAGT650.005865433729.1891546
CATGGAG700.00838988927.1256074
ATTCGCA350.00843789227.10421664-65
CATGGGG2059.440555E-1025.4716054
GAGTACT13950.022.44112212-13
GGTATCA23150.020.372621
AGTACTT14250.020.30438412-13
ATGGGAG1656.2968225E-520.1387065
GTACTTT15500.020.04400314-15
CATTTAT1200.004661008219.8495861
TACATGG9600.019.7790892
Produced by FastQC (version 0.11.5)