FastQCFastQC Report
Thu 26 May 2016
SRR938857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74707
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5710.7643192739636179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.6880212028323985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.6840055148781239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.4711740533015648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3490.46715836534729005No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.40826160868459455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.40826160868459455No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2090.2797595941478041No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1740.2329099013479326No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1740.2329099013479326No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1680.2248785254393832No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1660.22220140013653342No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1610.21550858687940888No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT1570.2101543362737093No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1410.18873733385091088No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1380.1847216458966362No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1350.1807059579423615No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA1350.1807059579423615No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1330.1780288326395117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.16598176877668758No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1180.1579503928681382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.14991901695958879No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1050.1405490783996145No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA1010.1351948277939149No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1000.13385626514249No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1000.13385626514249No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT920.1231477639310908No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT910.1218092012796659No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC870.1164549506739663No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC870.1164549506739663No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG750.1003921988568675No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG156.6482916E-494.802412
GGATATA250.005030976656.919571
GGAGTAC250.005057748456.8433768
CTCTAAA350.00837144327.1409454-55
AGCGGGG350.00848170627.06827510-11
TCGCCGT500.001634758323.78024394-95
GTACATG3550.022.714381
TACATGG3550.021.3639222
AGTACTT4200.020.30120512-13
GTCGCCG600.00472794619.8035692-93
GAGTACT4150.019.40436412-13
ACATGGG3305.838956E-1018.6732033
AGTACAT3054.5165507E-918.637177
GTACTTT4250.018.39050314-15
CATGGGA1901.8658435E-417.4636024
TACTTTT4700.017.1336414-15
ACTTTTT4850.016.60373516-17
TATACTC2304.2608255E-516.4873754
ATACTCT2205.702167E-415.0822025
AGAGTAC9950.013.33010410-11
Produced by FastQC (version 0.11.5)