##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938857_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74707 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72912846185766 31.0 31.0 34.0 30.0 34.0 2 31.973215361344987 33.0 31.0 34.0 30.0 34.0 3 32.03153653606757 33.0 31.0 34.0 30.0 34.0 4 35.71511371089724 37.0 35.0 37.0 33.0 37.0 5 35.60426733773274 37.0 35.0 37.0 33.0 37.0 6 35.6623743424311 37.0 35.0 37.0 33.0 37.0 7 35.575742567630876 37.0 35.0 37.0 33.0 37.0 8 35.57078988582061 37.0 35.0 37.0 33.0 37.0 9 37.13278541502135 39.0 37.0 39.0 33.0 39.0 10-11 37.07690711713762 39.0 37.0 39.0 33.0 39.0 12-13 36.99722917531155 39.0 37.0 39.0 33.0 39.0 14-15 38.035257740238535 40.0 37.0 41.0 33.0 41.0 16-17 37.94202015875353 40.0 37.0 41.0 32.5 41.0 18-19 38.01205375667608 40.0 37.0 41.0 33.0 41.0 20-21 38.13417752017884 40.0 37.0 41.0 33.0 41.0 22-23 38.08001927530218 40.0 37.0 41.0 33.0 41.0 24-25 37.915168591965944 40.0 37.0 41.0 32.0 41.0 26-27 37.65613664047545 40.0 36.5 41.0 32.0 41.0 28-29 37.409720641974644 39.5 36.0 41.0 32.0 41.0 30-31 37.208822466435535 39.0 36.0 41.0 31.0 41.0 32-33 36.83084583773943 39.0 35.0 41.0 30.5 41.0 34-35 36.65874014483248 39.0 35.0 41.0 30.0 41.0 36-37 36.4422276359645 39.0 35.0 41.0 30.0 41.0 38-39 36.10675706426439 39.0 35.0 41.0 30.0 41.0 40-41 35.959448244475084 39.0 35.0 41.0 30.0 41.0 42-43 35.73706613838061 39.0 35.0 41.0 28.5 41.0 44-45 35.417738632256686 39.0 35.0 41.0 26.5 41.0 46-47 34.92951798358922 38.0 34.5 40.5 24.5 41.0 48-49 34.50475189741256 38.0 34.0 40.0 23.0 41.0 50-51 34.01026677553643 38.0 33.0 40.0 20.0 41.0 52-53 33.775208481132964 38.0 33.0 40.0 19.0 41.0 54-55 33.45165780984379 38.0 33.0 40.0 15.0 41.0 56-57 32.81030559385332 37.5 32.5 40.0 5.0 41.0 58-59 32.134686173986374 37.0 31.5 40.0 2.0 41.0 60-61 31.294731417403995 36.0 30.5 40.0 2.0 41.0 62-63 30.57540792696802 36.0 29.5 39.0 2.0 41.0 64-65 29.834901682573253 35.0 28.5 39.0 2.0 40.0 66-67 28.879428969172903 35.0 27.0 38.5 2.0 40.0 68-69 28.20978623154457 35.0 25.5 38.0 2.0 40.0 70-71 27.68073272919539 34.5 24.5 37.0 2.0 39.5 72-73 27.332177707577603 34.0 24.0 37.0 2.0 39.0 74-75 26.96104113403028 34.0 22.0 36.0 2.0 39.0 76-77 25.12613275864377 31.5 19.5 34.5 2.0 36.5 78-79 26.01980403442783 33.5 20.0 35.0 2.0 37.0 80-81 26.053582662936538 34.0 20.0 35.0 2.0 37.0 82-83 25.74157709451591 33.5 18.0 35.0 2.0 36.5 84-85 25.439697752553307 33.0 13.5 35.0 2.0 36.0 86-87 24.972084275904535 33.0 6.5 35.0 2.0 35.5 88-89 24.493267029863333 32.5 2.0 35.0 2.0 35.0 90-91 24.08618335631199 32.0 2.0 35.0 2.0 35.0 92-93 23.854518318229886 32.0 2.0 35.0 2.0 35.0 94-95 23.563247085279826 32.0 2.0 35.0 2.0 35.0 96-97 23.084055041696228 32.0 2.0 34.0 2.0 35.0 98-99 22.45408060824287 31.0 2.0 34.0 2.0 35.0 100-101 19.996004390485496 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2450614576207144 1101 2 -0.329340192119588 1101 3 -0.008513047699999277 1101 4 0.11612134203792834 1101 5 0.5281971711667666 1101 6 0.24145271752745145 1101 7 -0.15548219793128482 1101 8 0.03139621172477547 1101 9 0.009175461399905771 1101 10-11 -0.3867089976460747 1101 12-13 -0.23535049669268204 1101 14-15 -0.0021650696440573824 1101 16-17 0.016246020203979583 1101 18-19 0.10813060513152095 1101 20-21 -0.43433201747429706 1101 22-23 -0.3794347970789431 1101 24-25 -0.7533542552948234 1101 26-27 -0.5122154405951562 1101 28-29 -0.6230061884558182 1101 30-31 -0.9806610865696612 1101 32-33 0.06036299783839638 1101 34-35 0.4327968797088886 1101 36-37 0.656622045507639 1101 38-39 -0.5017428207630985 1101 40-41 -0.47644564492895825 1101 42-43 0.12389171443370373 1101 44-45 -0.21377202097702508 1101 46-47 0.10187146228695809 1101 48-49 0.548712808110075 1101 50-51 0.007668590587485369 1101 52-53 0.2987159326414073 1101 54-55 0.600323081557498 1101 56-57 0.13853348048664316 1101 58-59 -1.0495030498256064 1101 60-61 -0.3176805634649007 1101 62-63 -0.2940238343290247 1101 64-65 -0.5430432793382707 1101 66-67 0.3776643572645284 1101 68-69 0.6683087145181048 1101 70-71 0.42322286183654256 1101 72-73 0.3551269317013883 1101 74-75 0.5694961445818372 1101 76-77 0.3043662013255002 1101 78-79 0.6830278752058341 1101 80-81 0.9809129169415165 1101 82-83 1.739451935676609 1101 84-85 1.8177488721406654 1101 86-87 2.2256223199625325 1101 88-89 2.041517400577 1101 90-91 1.8958400838129457 1101 92-93 1.691952628393114 1101 94-95 2.177104148121497 1101 96-97 1.9556486151856518 1101 98-99 1.8814222211171732 1101 100-101 1.796049594588336 1102 1 0.5229309666217112 1102 2 0.5532355654561698 1102 3 0.6188543765424228 1102 4 0.2524849784015615 1102 5 0.5182539893485867 1102 6 0.44410423267896704 1102 7 0.3913928020687152 1102 8 0.5044075753611423 1102 9 0.6862587947332415 1102 10-11 0.3000807750811916 1102 12-13 0.5799904123982245 1102 14-15 0.8304580364165517 1102 16-17 1.1204126868706439 1102 18-19 0.6538218930103099 1102 20-21 0.35520396737418736 1102 22-23 0.7621371726180257 1102 24-25 1.1379809719779033 1102 26-27 0.9636368321321171 1102 28-29 1.7313972206350883 1102 30-31 0.966071867975792 1102 32-33 1.411452012989912 1102 34-35 0.7448233948604042 1102 36-37 0.695684545507639 1102 38-39 1.4426227095399327 1102 40-41 2.0020107944649794 1102 42-43 2.278484517464001 1102 44-45 2.0248643426593347 1102 46-47 1.4922597198627159 1102 48-49 1.8399059899282548 1102 50-51 1.4437481360420321 1102 52-53 1.5399564629444384 1102 54-55 2.175133687618107 1102 56-57 2.3880599956381587 1102 58-59 2.0783378592653037 1102 60-61 2.777016406232068 1102 62-63 2.6986371505194597 1102 64-65 2.825801417631425 1102 66-67 2.6210355693857394 1102 68-69 3.3856382599726516 1102 70-71 3.1616224830486637 1102 72-73 2.916206477155935 1102 74-75 2.366134402157595 1102 76-77 2.357869989204289 1102 78-79 1.4256888600543185 1102 80-81 1.085316326032423 1102 82-83 1.6163458750705466 1102 84-85 2.0142450842618764 1102 86-87 2.0281791381443526 1102 88-89 2.0978620975466953 1102 90-91 2.46662606866143 1102 92-93 2.9589980829385674 1102 94-95 3.184679905697255 1102 96-97 3.5363777818523197 1102 98-99 3.572710099905052 1102 100-101 3.251542018830758 1103 1 0.2332350206757674 1103 2 0.0903977276183312 1103 3 -0.0031895423764964903 1103 4 0.2930255189421018 1103 5 0.06259520556480425 1103 6 -0.04069306461833122 1103 7 -0.5608876033366883 1103 8 -0.3211836408550752 1103 9 0.07223852446296775 1103 10-11 0.3184507075136267 1103 12-13 0.2886052772630947 1103 14-15 -0.3690774365564238 1103 16-17 -0.08060082664287194 1103 18-19 0.0581715551724713 1103 20-21 -0.1589429920852723 1103 22-23 0.12404595640180816 1103 24-25 -0.22878423072479848 1103 26-27 -0.604353032732746 1103 28-29 -0.443645009094638 1103 30-31 -0.16800752391610274 1103 32-33 -0.08664764917224943 1103 34-35 -0.07907863216662747 1103 36-37 -0.41770227881668376 1103 38-39 0.00828993926965893 1103 40-41 -0.5573219758052943 1103 42-43 -0.12037527983329 1103 44-45 -0.10689241409742323 1103 46-47 -0.2824446720291718 1103 48-49 -0.5735230641258013 1103 50-51 -0.08876875584985555 1103 52-53 0.15334328726876123 1103 54-55 -0.2936163123818929 1103 56-57 0.2077390496922078 1103 58-59 0.7244527241301739 1103 60-61 0.21958397379963301 1103 62-63 0.10974356943837904 1103 64-65 -0.6433708796658735 1103 66-67 0.6303261099262798 1103 68-69 0.5921416383510305 1103 70-71 0.9498403884540672 1103 72-73 0.5830139095883702 1103 74-75 0.5027475778332722 1103 76-77 -0.42802528106598103 1103 78-79 -0.17671823454027802 1103 80-81 0.5994632854918827 1103 82-83 0.7328999291246028 1103 84-85 0.07716738155917469 1103 86-87 0.023533867874082404 1103 88-89 -0.2615129024533047 1103 90-91 -0.3422844043115454 1103 92-93 -1.1237721873317028 1103 94-95 -1.0991038780865274 1103 96-97 -0.21552255598551895 1103 98-99 -0.3546547649598111 1103 100-101 1.2608325593712983 1104 1 -0.10023079808417279 1104 2 0.1782355654561698 1104 3 0.018670553013009794 1104 4 0.0318967431074455 1104 5 -0.08009159888671036 1104 6 -0.08163106143867793 1104 7 -0.34665866851951677 1104 8 0.02738551653761334 1104 9 0.5778029123802995 1104 10-11 0.033077098610604594 1104 12-13 -0.45125958760177554 1104 14-15 0.008307301122435717 1104 16-17 -0.22885201901170404 1104 18-19 -0.40546119522498003 1104 20-21 0.31522234972712937 1104 22-23 -0.11562018032314825 1104 24-25 -0.6143168221397488 1104 26-27 -0.7468411090443539 1104 28-29 -0.5227388087766798 1104 30-31 -0.923174455553621 1104 32-33 -1.174485487010088 1104 34-35 -2.471169252198422 1104 36-37 -2.505142660374709 1104 38-39 -2.4199692022247774 1104 40-41 -2.1620517055350206 1104 42-43 -2.271607394300709 1104 44-45 -2.3354297749877233 1104 46-47 -2.839082191901994 1104 48-49 -2.997410186542332 1104 50-51 -2.432170981605026 1104 52-53 -2.0533339782320326 1104 54-55 -2.3597927829701284 1104 56-57 -1.9713150043618413 1104 58-59 -2.074235670146461 1104 60-61 -1.9509247702385188 1104 62-63 -2.4929988788923048 1104 64-65 -2.949014758839162 1104 66-67 -2.7705085482613185 1104 68-69 -3.4038837988508774 1104 70-71 -3.404094428716043 1104 72-73 -2.739124405197007 1104 74-75 -2.172928097842405 1104 76-77 -2.128343246089827 1104 78-79 -2.515947169357446 1104 80-81 -1.823231468085222 1104 82-83 -2.129977654341218 1104 84-85 -1.6392475627969496 1104 86-87 -0.9782546853850604 1104 88-89 -1.3938658436297757 1104 90-91 -1.303134960750338 1104 92-93 -0.8093842700026066 1104 94-95 -0.24179068253803848 1104 96-97 -0.12768471814768034 1104 98-99 -0.15890754715377042 1104 100-101 0.7864684894189935 1105 1 0.3363238237645696 1105 2 0.3282355654561684 1105 3 0.3157293765424214 1105 4 0.4524849784015643 1105 5 0.503521846491445 1105 6 0.32803280410753644 1105 7 0.11594637349728742 1105 8 0.32360400393257294 1105 9 -0.2717769195524724 1105 10-11 0.27887541793833037 1105 12-13 0.15231184096965222 1105 14-15 0.36818125070227126 1105 16-17 0.7614841154420731 1105 18-19 -0.053990606989692935 1105 20-21 0.6148021816599041 1105 22-23 0.6759764583323147 1105 24-25 0.23820418626361572 1105 26-27 0.6176546892749784 1105 28-29 0.5646561492065132 1105 30-31 0.6310272251186504 1105 32-33 0.8112287987041924 1105 34-35 1.4550912520032568 1105 36-37 1.849479188364775 1105 38-39 1.4999887809685077 1105 40-41 1.8558054373221182 1105 42-43 1.4126363031782887 1105 44-45 1.7355786283736236 1105 46-47 1.995161505577002 1105 48-49 1.4591024184996826 1105 50-51 1.5959802788991766 1105 52-53 2.9075903915158676 1105 54-55 2.8349551161895334 1105 56-57 3.0251135670667253 1105 58-59 3.3095878592653065 1105 60-61 2.987730691946357 1105 62-63 2.7765389362337487 1105 64-65 3.289640703345711 1105 66-67 2.8040712836714548 1105 68-69 2.563763259972653 1105 70-71 2.7372921259058067 1105 72-73 2.7577243342987927 1105 74-75 2.6699290450147366 1105 76-77 2.5779592749185767 1105 78-79 1.7821620743400324 1105 80-81 2.9042895403181355 1105 82-83 2.9788458750705473 1105 84-85 2.429870084261875 1105 86-87 3.4295184238586387 1105 88-89 3.2527728118324077 1105 90-91 2.9878314258042877 1105 92-93 3.353640940081423 1105 94-95 2.7346799056972557 1105 96-97 3.443743853280889 1105 98-99 3.4387815284764827 1105 100-101 3.356006304545044 1106 1 -0.049390461949716524 1106 2 -0.22890729168668855 1106 3 -0.3699849091718619 1106 4 0.00962783554442126 1106 5 0.060664703634301986 1106 6 0.08517566125039622 1106 7 -0.484053626502714 1106 8 -0.7192531389245715 1106 9 -0.5003483481239002 1106 10-11 -0.35683886777595575 1106 12-13 -0.5976881590303478 1106 14-15 -0.38896160644059563 1106 16-17 -1.0313730274150714 1106 18-19 -0.30399060698969294 1106 20-21 0.07908789594561227 1106 22-23 -0.695452113096259 1106 24-25 -0.9046529565935302 1106 26-27 -0.6823453107250259 1106 28-29 -0.10677242222205052 1106 30-31 0.2738843679757892 1106 32-33 -0.5244854870100895 1106 34-35 -0.05919446228244851 1106 36-37 -0.14337795449235813 1106 38-39 -0.17143979046007018 1106 40-41 0.6486625801792627 1106 42-43 0.4840648746068581 1106 44-45 -0.45013565734066674 1106 46-47 0.02373293414842692 1106 48-49 -0.6051832957860341 1106 50-51 -0.31830543538653444 1106 52-53 0.04330467723015374 1106 54-55 0.1778122590466822 1106 56-57 -0.07488643293326902 1106 58-59 1.016730716408162 1106 60-61 0.5663021205177863 1106 62-63 0.2979675076623174 1106 64-65 0.13964070334571232 1106 66-67 1.075499855100027 1106 68-69 1.4137632599726508 1106 70-71 1.437292125905806 1106 72-73 1.3934386200130753 1106 74-75 1.1699290450147366 1106 76-77 0.8565307034900016 1106 78-79 1.3821620743400338 1106 80-81 1.5114323974609931 1106 82-83 1.6574173036419744 1106 84-85 1.751298655690448 1106 86-87 1.700946995287211 1106 88-89 1.9527728118324106 1106 90-91 1.6949742829471433 1106 92-93 2.7250695115099965 1106 94-95 1.8275370485543974 1106 96-97 2.7937438532808905 1106 98-99 3.953067242762195 1106 100-101 1.7631491616879025 1107 1 -0.6922476048068589 1107 2 -0.04319300597239817 1107 3 -0.269984909171864 1107 4 -0.10465787874129262 1107 5 -0.1964781535085578 1107 6 -0.7005386244638885 1107 7 -0.2983393407884307 1107 8 -0.0906817103531452 1107 9 -0.21463406240961547 1107 10-11 -0.1996960106330974 1107 12-13 0.2594546981125134 1107 14-15 0.18246696498798087 1107 16-17 0.5543412582992104 1107 18-19 0.06743796443888073 1107 20-21 -0.2851978183401016 1107 22-23 -0.49545211309626325 1107 24-25 -0.0403672423078163 1107 26-27 -0.4394881678678857 1107 28-29 -0.2567724222220562 1107 30-31 -1.0332584891670606 1107 32-33 -0.4030569155815158 1107 34-35 -0.8306230337110208 1107 36-37 0.28519347407906537 1107 38-39 0.742845923825648 1107 40-41 0.6486625801792627 1107 42-43 0.3054934460354275 1107 44-45 -0.7287070859120988 1107 46-47 -0.10483849442299942 1107 48-49 0.10910241849968116 1107 50-51 0.40312313604203354 1107 52-53 0.40044753437300784 1107 54-55 -0.6721877409533263 1107 56-57 -0.06774357579040924 1107 58-59 -1.3546978550204116 1107 60-61 -1.0194121651965027 1107 62-63 -0.6020324923376847 1107 64-65 -0.7175021537971489 1107 66-67 -0.5887858591856912 1107 68-69 0.09233468854408144 1107 70-71 0.5444349830486637 1107 72-73 0.10772433429879058 1107 74-75 0.519929045014738 1107 76-77 0.4922449892042877 1107 78-79 -0.38926649708853844 1107 80-81 -0.12428188825328945 1107 82-83 0.500274446499116 1107 84-85 0.40844151283330277 1107 86-87 0.15094699528721023 1107 88-89 0.31705852611812446 1107 90-91 1.0878314258042856 1107 92-93 0.7607837972242812 1107 94-95 0.7061084771258272 1107 96-97 0.5580295675666029 1107 98-99 -0.3540756143806618 1107 100-101 -2.0654222668835267 1108 1 -0.9154136279728817 1108 2 -0.7609536237330197 1108 3 -0.5707571099440649 1108 4 -0.03129880538222096 1108 5 0.04908169205128843 1108 6 -0.01366603759130669 1108 7 0.03370699125790111 1108 8 -0.6860485057199384 1108 9 -0.49532904310459713 1108 10-11 -0.31668442762151017 1108 12-13 -0.42085418219637205 1108 14-15 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52-53 -0.7524417112128674 2316 54-55 -0.8076612562021168 2316 56-57 -0.09912399312588605 2316 58-59 0.5562170727484528 2316 60-61 0.758757979265777 2316 62-63 0.5419482460250791 2316 64-65 1.0359488895415367 2316 66-67 0.6010215244419186 2316 68-69 0.35983067570298743 2316 70-71 0.9685922864194474 2316 72-73 0.3022668704143605 2316 74-75 0.7624651605845614 2316 76-77 0.8366270116761996 2316 78-79 0.9684381578071282 2316 80-81 1.0529251743470276 2316 82-83 0.8080593582166138 2316 84-85 1.0130161516776077 2316 86-87 0.8247030145488452 2316 88-89 -0.08454661031847621 2316 90-91 0.021138006863676395 2316 92-93 -0.5276592204322199 2316 94-95 -0.09902795947128595 2316 96-97 -0.13892067320385948 2316 98-99 -0.4055202371735973 2316 100-101 -0.2375731497085667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 4.0 6 20.0 7 41.0 8 84.0 9 118.0 10 200.0 11 328.0 12 386.0 13 603.0 14 647.0 15 840.0 16 1110.0 17 1376.0 18 1458.0 19 1177.0 20 1213.0 21 1294.0 22 1468.0 23 1449.0 24 1589.0 25 1707.0 26 1435.0 27 1108.0 28 1344.0 29 1578.0 30 1838.0 31 2297.0 32 2634.0 33 3275.0 34 4178.0 35 5686.0 36 8151.0 37 12492.0 38 10550.0 39 1027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.5141678646764 21.63404049496836 16.207392279890904 22.644399360464334 2 14.539467519777263 22.352657716144403 39.94672520647329 23.161149557605043 3 19.414512696266748 26.872983790006295 35.1412852878579 18.571218225869064 4 11.630770878230956 21.31928734924438 34.810660312955946 32.239281459568716 5 11.181013827352189 39.138233365012645 34.92443813832706 14.756314669308097 6 25.719142784478027 37.7862850870735 17.656979934945856 18.837592193502616 7 21.355428540832854 37.17188483006947 20.316703923327132 21.155982705770544 8 23.43957058910142 33.624693803793484 21.46652924090112 21.46920636620397 9 21.52944168551809 18.370433828155328 21.801169903757344 38.29895458256924 10-11 22.135141285287858 26.528973188590093 27.997376417203206 23.338509108918842 12-13 26.00492591055724 24.38660366498454 26.901762887011927 22.70670753744629 14-15 23.100914238290922 26.78999290561795 26.152837083539694 23.956255772551437 16-17 20.421917771545985 30.912303480955472 27.264695843065866 21.40108290443268 18-19 19.28935708835852 28.56358841875594 29.230861900491252 22.91619259239429 20-21 19.99946457493943 26.322165258944946 28.69476755859558 24.983602607520044 22-23 19.025660246027815 28.57095051333878 29.141178202845786 23.262211037787623 24-25 21.019449315325204 29.234877588445528 30.02864524074049 19.717027855488777 26-27 20.590439985543522 27.218332954073915 31.85645254126119 20.334774519121368 28-29 16.917424070033597 26.208387433573826 32.9105706292583 23.96361786713427 30-31 17.558595580066125 26.488816309047348 32.87041374971555 23.082174361170974 32-33 18.401890050463813 27.778521423695235 32.35439784759125 21.465190678249694 34-35 18.833372377095813 28.53518958535524 32.74455339513403 19.886884642414913 36-37 19.799448416204783 27.105925509411733 31.163145634187483 21.931480440196 38-39 20.709528431339738 25.016901502727666 32.42143311355802 21.852136952374575 40-41 18.984350945770473 26.071270799587687 33.916547301910285 21.02783095273156 42-43 19.982999457856724 26.480017669854828 33.13432436231234 20.402658509976103 44-45 18.38431162572786 27.64674385917944 31.89077036342949 22.07817415166321 46-47 21.772682247744495 25.356062431397746 29.28144995047252 23.589805370385246 48-49 20.579731484332125 25.316904707724845 31.03926004256629 23.06410376537674 50-51 19.02377972465582 26.99430437779845 32.155167221058406 21.82674867648732 52-53 18.65621695423454 28.137925495602822 33.13678771734911 20.069069832813526 54-55 17.586705395746048 27.97060516417471 31.4803164362108 22.962373003868446 56-57 20.587762860240673 29.468456771119172 27.999384261180342 21.94439610745981 58-59 18.897411185179237 30.305464085883326 30.477471689020962 20.31965303991647 60-61 19.332449870157685 32.4458544159773 27.913420608786442 20.308275105078575 62-63 18.353032513686802 33.33690283373713 27.496753985570294 20.81331066700577 64-65 18.02709250806484 33.8241396388558 27.765805078506702 20.382962774572665 66-67 18.051976735625413 32.34658296132197 27.34233299645948 22.259107306593133 68-69 17.35324235508289 32.16119079330447 27.82154163292351 22.664025218689137 70-71 18.677339687171628 30.365903447537328 27.160650822228614 23.796106043062426 72-73 18.29279719437268 29.002636968423307 28.15867321669991 24.5458926205041 74-75 17.925361746556547 29.587588847095986 28.10579999196862 24.38124941437884 76-77 18.61551035720985 28.257898544899106 28.445480612053487 24.681110485837554 78-79 18.20525181108478 27.854550810803573 29.104567549110193 24.83562982900146 80-81 18.079296451470412 28.43173999759059 28.528116508493177 24.96084704244582 82-83 17.96953431405357 27.781198548998088 28.58567470250445 25.663592434443892 84-85 18.118784049687445 27.105893691354222 28.521423695236052 26.253898563722277 86-87 18.461847793304514 27.084212995543044 28.453470057973927 26.000469153178514 88-89 18.58986410270183 26.301230058548636 28.322903797604337 26.786002041145192 90-91 17.918198652746838 26.007756098870132 29.023644024583344 27.050401223799685 92-93 17.869811396522415 26.38641626621334 28.783112693589626 26.960659643674624 94-95 17.586705395746048 26.106656672065537 28.659964929658532 27.64667300252988 96-97 17.14966469005582 25.676978060958145 28.637209364584308 28.53614788440173 98-99 17.68308190664864 25.58796364463839 28.17607453116843 28.552879917544537 100-101 17.71343667175327 25.17669263512971 28.9695606778572 28.140310015259818 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 270.0 1 173.5 2 81.0 3 76.0 4 72.5 5 72.5 6 106.5 7 169.0 8 233.5 9 256.0 10 216.5 11 187.0 12 174.5 13 173.5 14 189.5 15 210.0 16 202.5 17 198.5 18 210.0 19 231.0 20 261.0 21 300.0 22 331.0 23 358.0 24 388.5 25 451.5 26 557.0 27 671.0 28 757.5 29 822.5 30 921.5 31 1015.5 32 1143.5 33 1282.5 34 1435.5 35 1505.0 36 1604.5 37 1767.0 38 1798.5 39 1826.0 40 1922.5 41 2077.5 42 2274.5 43 2524.0 44 2901.0 45 3278.5 46 3655.0 47 4125.0 48 4624.5 49 4431.0 50 3611.5 51 2865.5 52 2148.0 53 1730.0 54 1515.0 55 1343.0 56 1200.5 57 1005.0 58 845.0 59 729.5 60 631.5 61 538.0 62 433.5 63 330.0 64 257.0 65 212.0 66 178.5 67 148.5 68 112.0 69 81.0 70 73.5 71 73.5 72 64.5 73 54.0 74 38.0 75 25.0 76 22.5 77 19.0 78 11.0 79 7.5 80 7.0 81 5.5 82 4.0 83 1.5 84 1.0 85 2.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3721204170961222 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.6928132571245E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006023531931412049 36-37 0.0174013144685237 38-39 0.012716345188536548 40-41 0.0080313759085494 42-43 0.0046849692799871495 44-45 0.0026771253028498 46-47 0.0013385626514249 48-49 0.0 50-51 6.6928132571245E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0013385626514249 60-61 0.0013385626514249 62-63 0.0 64-65 0.0 66-67 6.6928132571245E-4 68-69 6.6928132571245E-4 70-71 0.00200784397713735 72-73 0.0 74-75 0.0 76-77 0.09771507355401769 78-79 0.037479754239897196 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.13987979707390205 88-89 0.3199164736905511 90-91 0.24763409051360652 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0013385626514249 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 74707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.17424070033597 #Duplication Level Percentage of deduplicated Percentage of total 1 90.66914354270676 62.719691595165116 2 6.5714617438755365 9.09151752847792 3 1.3197105151128141 2.7386991848153452 4 0.5069855644568289 1.402813658693295 5 0.20511629707032006 0.709438205255197 6 0.16060993072487326 0.6666042004096002 7 0.08901273269089362 0.43101717375881776 8 0.06385696040868455 0.3533805399761736 9 0.048376485158094354 0.3011765965706025 >10 0.2612330198537095 3.3075883116709273 >50 0.034831069313827935 1.7454856974580695 >100 0.05805178218971323 7.6739796806189515 >500 0.0077402376252950975 3.7104956697498226 >1k 0.0038701188126475487 5.1481119573801655 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2561 3.428058950299169 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1285 1.7200530070809965 RNA PCR Primer, Index 39 (95% over 23bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 956 1.2796658947622044 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 695 0.9303010427403055 RNA PCR Primer, Index 39 (96% over 25bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 610 0.8165232173691891 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 511 0.6840055148781239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 497 0.6652656377581753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 346 0.46314267739301535 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 337 0.4510956135301913 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 320 0.42834004845596796 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 312 0.41763154724456875 RNA PCR Primer, Index 39 (96% over 28bp) TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 268 0.3587347905818732 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 266 0.3560576652790234 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 229 0.3065308471763021 Illumina PCR Primer Index 11 (96% over 25bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 190 0.25432690377073097 No Hit TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT 184 0.2462955278621816 RNA PCR Primer, Index 39 (95% over 22bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 184 0.2462955278621816 No Hit CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 181 0.24227983990790686 RNA PCR Primer, Index 39 (96% over 30bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 177 0.23692558930220728 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 165 0.2208628374851085 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 160 0.21417002422798398 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 155 0.2074772109708595 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 154 0.20613864831943457 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 147 0.19676870975946031 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 144 0.19275302180518558 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 139 0.1860602085480611 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.1793673952909366 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.1766902699880868 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 129 0.1726745820338121 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 125 0.16732033142811248 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 117 0.1566118302167133 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA 112 0.14991901695958879 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 112 0.14991901695958879 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 111 0.1485804543081639 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 105 0.1405490783996145 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 101 0.1351948277939149 No Hit CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 100 0.13385626514249 RNA PCR Primer, Index 39 (95% over 21bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 96 0.1285020145367904 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 95 0.12716345188536549 No Hit ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC 89 0.1191320759768161 Illumina PCR Primer Index 11 (96% over 26bp) GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 89 0.1191320759768161 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 83 0.11110070006826671 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 78 0.10440788681114219 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0026771253028497997 0.0 18-19 0.0 0.0 0.0 0.0046849692799871495 0.0 20-21 0.0 0.0 0.0 0.00870065723426185 0.0 22-23 0.0 0.0 0.0 0.0160627518170988 0.0 24-25 0.0 0.0 0.0 0.02342484639993575 0.0 26-27 0.0 0.0 0.0 0.0348026289370474 0.0 28-29 0.0 0.0 0.0 0.07964447775978155 0.0 30-31 0.0 0.0 0.0 0.16531248745097515 0.0 32-33 0.0 0.0 0.0 0.34534916406762417 0.0 34-35 0.0 0.0 0.0 0.675304857643862 0.0 36-37 0.0 0.0 0.0 1.1411246603397271 0.0 38-39 0.0 0.0 0.0 1.5922202738699185 0.0 40-41 0.0 0.0 0.0 2.025914572931586 0.0 42-43 0.0 0.0 0.0 2.4770101864617775 0.0 44-45 0.0 0.0 0.0 3.025151592220274 0.0 46-47 0.0 0.0 0.0 3.6301819106643287 0.0 48-49 0.0 0.0 0.0 4.170961221839988 0.0 50-51 0.0 0.0 0.0 4.642804556467265 0.0 52-53 0.0 0.0 0.0 5.095238732648881 0.0 54-55 0.0 0.0 0.0 5.699599769767223 0.0 56-57 0.0 0.0 0.0 6.428447133468082 0.0 58-59 0.0 0.0 0.0 7.210837003225937 0.0 60-61 0.0 0.0 0.0 7.992557591658077 0.0 62-63 0.0 0.0 0.0 8.652468978810553 0.0 64-65 0.0 0.0 0.0 9.3592300587629 0.0 66-67 0.0 0.0 0.0 10.165044774920691 0.0 68-69 0.0 0.0 0.0 11.049165406186837 0.0 70-71 0.0 0.0 0.0 12.01694620316704 0.0 72-73 0.0 0.0 0.0 12.93185377541596 0.0 74-75 0.0 0.0 0.0 13.908335229630424 0.0 76-77 0.0 0.0 0.0 14.846667648279277 0.0 78-79 0.0 0.0 0.0 15.916179206767772 0.0 80-81 0.0 0.0 0.0 16.999076391770515 0.0 82-83 0.0 0.0 0.0 18.056540886396185 0.0 84-85 0.0 0.0 0.0 19.219082549158713 0.0 86-87 0.0 0.0 0.0 20.447213781841057 0.0 88-89 0.0 0.0 0.0 21.58967700483221 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 25 0.004982287 57.05956 1 CTCCTGC 50 0.0015979199 38.039707 1 CAGAACC 50 0.0016137983 37.96332 4 ATGGGAT 65 1.3238312E-4 36.503193 7 AGTACAT 270 0.0 29.878538 2 TGGGATT 65 0.005837096 29.202555 8 CATGGGG 90 8.9192984E-4 26.363417 4 CAGACAC 45 8.9613936E-4 26.363417 18-19 CATGGGA 165 8.268944E-8 25.884083 4 TTAGAAG 50 0.0016562266 23.727076 92-93 GTACATG 335 0.0 22.710272 1 AGAATAA 65 2.9441225E-4 21.901917 68-69 TACATGG 335 1.8189894E-12 21.248127 2 ACATGGG 320 1.6370905E-11 20.76119 3 GAGTACA 535 0.0 18.664341 1 GAGTACT 490 0.0 17.432138 12-13 GTACTTT 545 0.0 16.10829 14-15 CTTGAAA 770 0.0 16.02348 56-57 GCTTGAA 765 0.0 15.507892 56-57 TGCTTGA 800 0.0 15.422599 54-55 >>END_MODULE