##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73571 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.778322980522216 31.0 31.0 34.0 30.0 34.0 2 31.993054328471818 33.0 31.0 34.0 30.0 34.0 3 32.04880999306792 33.0 31.0 34.0 30.0 34.0 4 35.730111049190576 37.0 35.0 37.0 33.0 37.0 5 35.61146375609955 37.0 35.0 37.0 33.0 37.0 6 35.64201927389868 37.0 35.0 37.0 33.0 37.0 7 35.54183034075927 37.0 35.0 37.0 33.0 37.0 8 35.57305188185562 37.0 35.0 37.0 33.0 37.0 9 37.11989778581234 39.0 37.0 39.0 33.0 39.0 10-11 37.05531391444998 39.0 37.0 39.0 33.0 39.0 12-13 36.98546302211469 39.0 37.0 39.0 33.0 39.0 14-15 38.02790501692243 40.0 37.0 41.0 33.0 41.0 16-17 37.91567329518424 40.0 37.0 41.0 32.0 41.0 18-19 38.0893558603254 40.0 37.0 41.0 33.0 41.0 20-21 38.14159111606475 40.0 37.0 41.0 33.0 41.0 22-23 38.034279811338706 40.0 37.0 41.0 33.0 41.0 24-25 37.88538282747278 40.0 37.0 41.0 32.5 41.0 26-27 37.59249568444088 40.0 36.5 41.0 32.0 41.0 28-29 37.324353345747646 39.5 36.0 41.0 31.0 41.0 30-31 37.12887550801267 39.0 36.0 41.0 31.0 41.0 32-33 36.817122235663504 39.0 35.0 41.0 31.0 41.0 34-35 36.60225496459203 39.0 35.0 41.0 30.0 41.0 36-37 36.343484525152576 39.0 35.0 41.0 30.0 41.0 38-39 35.95256962661918 39.0 35.0 41.0 30.0 41.0 40-41 35.79699202131275 39.0 35.0 41.0 29.0 41.0 42-43 35.52587296625029 38.5 35.0 41.0 27.5 41.0 44-45 35.173308776555984 38.0 35.0 40.5 26.0 41.0 46-47 34.67007380625518 38.0 34.0 40.0 23.5 41.0 48-49 34.218686710796376 38.0 34.0 40.0 22.0 41.0 50-51 33.72217313887265 38.0 33.0 40.0 18.5 41.0 52-53 33.44373462369684 38.0 33.0 40.0 14.0 41.0 54-55 33.04451482241644 38.0 33.0 40.0 8.0 41.0 56-57 32.40035475934811 37.0 32.0 40.0 2.0 41.0 58-59 31.72012069973223 37.0 31.0 40.0 2.0 41.0 60-61 30.887210993462098 36.0 30.5 39.5 2.0 41.0 62-63 30.152947492898015 35.5 28.5 39.0 2.0 41.0 64-65 29.38290223049843 35.0 28.0 39.0 2.0 40.0 66-67 28.53851381658534 35.0 26.0 38.0 2.0 40.0 68-69 28.043434233597477 35.0 25.5 38.0 2.0 40.0 70-71 27.580541245871334 34.5 23.5 37.0 2.0 39.5 72-73 27.10918704380802 34.0 22.0 36.5 2.0 39.0 74-75 26.687356431202513 34.0 20.5 36.0 2.0 39.0 76-77 24.977552296421145 31.5 18.0 34.5 2.0 36.0 78-79 25.760007339848585 33.0 18.0 35.0 2.0 37.0 80-81 25.782733685827296 33.0 18.0 35.0 2.0 37.0 82-83 25.47339984504764 33.0 12.5 35.0 2.0 36.0 84-85 25.13929401530494 33.0 7.0 35.0 2.0 36.0 86-87 24.63351048646885 33.0 2.0 35.0 2.0 35.5 88-89 24.046873088581098 32.0 2.0 35.0 2.0 35.0 90-91 23.592019953514292 32.0 2.0 35.0 2.0 35.0 92-93 23.358171018471953 31.5 2.0 34.0 2.0 35.0 94-95 23.054545948811352 31.0 2.0 34.0 2.0 35.0 96-97 22.51603892838211 31.0 2.0 34.0 2.0 35.0 98-99 21.812657161109676 31.0 2.0 34.0 2.0 35.0 100-101 18.91994807736744 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.026301731274084972 1101 2 0.26381108416401844 1101 3 -0.11007848802164588 1101 4 -0.24275371401944312 1101 5 0.15142784639611762 1101 6 6.228482257455425E-4 1101 7 0.16538636831535314 1101 8 0.1043981747640359 1101 9 -0.05293785134393403 1101 10-11 0.07627839603444642 1101 12-13 0.4859843698010238 1101 14-15 0.31711796069146203 1101 16-17 0.21898150061723953 1101 18-19 0.4097217307926968 1101 20-21 0.4290141875857998 1101 22-23 0.21092505691505892 1101 24-25 -0.19737996445349637 1101 26-27 0.1327749979292392 1101 28-29 0.7314872072947054 1101 30-31 0.5559195193171504 1101 32-33 0.8449520864811007 1101 34-35 0.5015920711845538 1101 36-37 0.419008455253838 1101 38-39 1.4348485047834245 1101 40-41 1.4397664444159446 1101 42-43 1.5918340432117688 1101 44-45 1.9113499940467733 1101 46-47 1.6997321130552976 1101 48-49 0.9941446388581028 1101 50-51 1.4552261877696182 1101 52-53 1.1302595189396385 1101 54-55 1.8170334978733749 1101 56-57 2.0492677228037124 1101 58-59 1.67221766118411 1101 60-61 1.665120336162122 1101 62-63 1.2362997024633415 1101 64-65 0.6676295961917518 1101 66-67 0.2877012200953608 1101 68-69 0.5031710961965423 1101 70-71 0.5098468243969378 1101 72-73 0.4763086773955578 1101 74-75 0.014776537608842233 1101 76-77 -0.4499964632264266 1101 78-79 0.11348343151249907 1101 80-81 0.736520782398383 1101 82-83 0.5642475064808856 1101 84-85 0.17027464468302966 1101 86-87 -0.04250481814306184 1101 88-89 0.6047558195994434 1101 90-91 1.1398197432976254 1101 92-93 -0.4473388626514705 1101 94-95 -1.6154608386843208 1101 96-97 -1.0659126967412682 1101 98-99 -0.6217616619379136 1101 100-101 -0.6355111818381189 1102 1 -0.048325134397558145 1102 2 0.03993048714909264 1102 3 -0.2891829656335858 1102 4 -0.1979775946164608 1102 5 -0.25155722823075166 1102 6 -0.4172875995354488 1102 7 0.21016248771833546 1102 8 -0.2836615267285012 1102 9 0.2604949844769635 1102 10-11 -0.03566190247301648 1102 12-13 -0.3647618988556971 1102 14-15 -0.13064323333838956 1102 16-17 -0.5646005889350008 1102 18-19 -0.6574424483117767 1102 20-21 -0.16053805122016485 1102 22-23 0.2631638628852073 1102 24-25 0.4220230206211326 1102 26-27 -0.8224488826677785 1102 28-29 0.26133795356336265 1102 30-31 0.4290538476753625 1102 32-33 0.0912207431975176 1102 34-35 -0.013333301949778331 1102 36-37 -0.3645736342984023 1102 38-39 0.06171417642521959 1102 40-41 -0.09008430185271266 1102 42-43 -0.7365241657434538 1102 44-45 0.5083649194199111 1102 46-47 -0.1435514690342572 1102 48-49 -0.2819747641269714 1102 50-51 -0.7611917226781415 1102 52-53 -1.1234718243439445 1102 54-55 -1.048638143917671 1102 56-57 -1.4059561577933053 1102 58-59 -1.2307674134427558 1102 60-61 -0.7229393653304221 1102 62-63 -1.6666853721635206 1102 64-65 -1.2353554784351175 1102 66-67 -1.5182689291583706 1102 68-69 -1.414739351564652 1102 70-71 -1.2214964591851505 1102 72-73 -1.881900277828322 1102 74-75 -1.9180592832866772 1102 76-77 -1.30820541845031 1102 78-79 -1.520844926696455 1102 80-81 -1.5619866802881859 1102 82-83 -1.6223196576982204 1102 84-85 -0.6804716239736841 1102 86-87 -0.8484749673967968 1102 88-89 -1.559423284878168 1102 90-91 -0.7034638387919259 1102 92-93 -0.8503239372783398 1102 94-95 -0.09307277898282607 1102 96-97 0.8967738704229085 1102 98-99 0.5946562485098461 1102 100-101 1.4540410569678457 1103 1 0.28278852805938826 1103 2 0.44222669840053186 1103 3 0.4125391928049922 1103 4 0.28147131468319486 1103 5 0.34545769714238617 1103 6 0.3145149262969298 1103 7 0.20327385396402775 1103 8 0.41874949508550685 1103 9 0.6887383828696159 1103 10-11 0.3829374086636079 1103 12-13 0.47082937554154825 1103 14-15 0.5157402339405976 1103 16-17 0.40279321129462176 1103 18-19 0.4353244862461949 1103 20-21 0.1340658523389564 1103 22-23 0.35225685944088525 1103 24-25 0.19412405391619103 1103 26-27 0.32588636417493433 1103 28-29 1.0200061510375278 1103 30-31 1.0465050531862659 1103 32-33 0.848396403358251 1103 34-35 0.6945886268676702 1103 36-37 -1.2507963667897926 1103 38-39 -1.1702031599697378 1103 40-41 -0.692954565917006 1103 42-43 -0.44295355724747054 1103 44-45 -0.7087418544032786 1103 46-47 -0.5668580132363203 1103 48-49 -0.0838117331281154 1103 50-51 -0.2854167513807866 1103 52-53 -0.20728353502132535 1103 54-55 -0.43348314965820123 1103 56-57 -0.15371735182315405 1103 58-59 0.4063163982679221 1103 60-61 0.7639722305363996 1103 62-63 0.3540953396619635 1103 64-65 1.0825549693260754 1103 66-67 1.0106633326097132 1103 68-69 1.0169483637051506 1103 70-71 0.8953806935128981 1103 72-73 1.371142202079831 1103 74-75 1.6829740117764693 1103 76-77 1.235078163639244 1103 78-79 1.3107279780107781 1103 80-81 1.3059811727542936 1103 82-83 1.1721694352983363 1103 84-85 1.5864629340056489 1103 86-87 1.943258672098036 1103 88-89 1.7011966921597228 1103 90-91 1.763241098062263 1103 92-93 1.8827415047423273 1103 94-95 1.515767634335198 1103 96-97 2.311010380181809 1103 98-99 3.2098112427693195 1103 100-101 3.2631110914110195 1104 1 0.03896568423654401 1104 2 -0.443787287613457 1104 3 -0.20121372094792278 1104 4 -0.17400654079465738 1104 5 -0.5787847271000359 1104 6 -0.276627264845267 1104 7 -0.8235326728424965 1104 8 -0.8695954932594816 1104 9 -0.8695366754054419 1104 10-11 -0.2992304235042198 1104 12-13 -0.8245086197964469 1104 14-15 -0.6911362229358531 1104 16-17 -0.6942930357916239 1104 18-19 -0.7329738820521712 1104 20-21 -0.44728612901302967 1104 22-23 -0.32175246456843354 1104 24-25 -0.5955029857108443 1104 26-27 0.06877680706537603 1104 28-29 -0.7590148279834423 1104 30-31 -1.0093224526412428 1104 32-33 -0.6230488180869713 1104 34-35 -0.6495838673048198 1104 36-37 -0.8577893737828006 1104 38-39 -0.80248754225412 1104 40-41 -0.5649825379449709 1104 42-43 0.8815219672280463 1104 44-45 0.9658502201887984 1104 46-47 0.12906273268442447 1104 48-49 -0.828333877650266 1104 50-51 -1.2466055625695986 1104 52-53 -1.0435306212684097 1104 54-55 -0.5942057603808024 1104 56-57 -0.6643234124292192 1104 58-59 -0.7920519001003754 1104 60-61 -1.2218086552444838 1104 62-63 -0.9329675974009746 1104 64-65 -1.6204753337042277 1104 66-67 -1.0797795578331773 1104 68-69 -0.35368100692421933 1104 70-71 -0.40636755823535253 1104 72-73 -0.1285081475705212 1104 74-75 -0.1864898576874019 1104 76-77 -0.017952139391059774 1104 78-79 -0.5469643296815292 1104 80-81 -0.3163964496233298 1104 82-83 -0.6326091194802181 1104 84-85 -0.7952386976959822 1104 86-87 -1.4964849176455495 1104 88-89 -0.734117993154964 1104 90-91 -0.022889438068272483 1104 92-93 -0.5688901968893738 1104 94-95 -1.221295302727743 1104 96-97 -0.3840945149230883 1104 98-99 -0.2766689437108667 1104 100-101 0.10623463453455884 1105 1 -0.6036368113071262 1105 2 -0.2552846138166629 1105 3 -0.5216237031226107 1105 4 -0.41242008803173746 1105 5 -0.2280717146223168 1105 6 -0.06851674791121809 1105 7 -0.03654515056085472 1105 8 -0.6708432650954919 1105 9 -0.31918016916658587 1105 10-11 -0.11808069088390027 1105 12-13 -0.5655959638249684 1105 14-15 -0.4836941551996716 1105 16-17 -0.8946049787506283 1105 18-19 -0.5767795861519929 1105 20-21 -0.8601649168918115 1105 22-23 -0.8750055839980249 1105 24-25 -0.5903782085272482 1105 26-27 -0.3712855815264291 1105 28-29 -0.14537846434708257 1105 30-31 -0.45428680201735006 1105 32-33 -0.3552235061440143 1105 34-35 -0.21197245910517637 1105 36-37 -0.01086424009296394 1105 38-39 -0.24656062603308015 1105 40-41 -1.195330131527868 1105 42-43 -0.49815717715697616 1105 44-45 -0.1562531666204734 1105 46-47 -1.1949123118788592 1105 48-49 -1.016390918648483 1105 50-51 -0.6797606427834992 1105 52-53 -0.7451794626231347 1105 54-55 -0.055881339703447 1105 56-57 -0.8684232341760989 1105 58-59 -0.24481482345153438 1105 60-61 -0.3196250545314747 1105 62-63 -0.41558791825658403 1105 64-65 0.3443196752084283 1105 66-67 0.16643256337894385 1105 68-69 -0.07694303900978028 1105 70-71 -0.4097098042246614 1105 72-73 0.07068971339204566 1105 74-75 -0.3634060787212725 1105 76-77 -0.03109830694899074 1105 78-79 -0.14990550615211617 1105 80-81 -0.06505955122760909 1105 82-83 -0.39909753302745443 1105 84-85 -0.54702122889028 1105 86-87 -0.3518770745082982 1105 88-89 -0.9854548915506882 1105 90-91 -1.9449036983178232 1105 92-93 -1.4175978617735083 1105 94-95 -1.6450920941715914 1105 96-97 -1.2497362261530327 1105 98-99 -1.9861163590406328 1105 100-101 -0.8039703565527816 1106 1 0.2609033709629607 1106 2 0.28924728344928496 1106 3 0.059776462577474376 1106 4 0.3185244024405094 1106 5 0.5989788239029465 1106 6 0.3554683390813267 1106 7 0.3354515354374854 1106 8 0.35981125023177185 1106 9 0.3355422831946413 1106 10-11 0.5500013306571105 1106 12-13 0.14753576774089794 1106 14-15 -0.018387610046403324 1106 16-17 0.26270653740512984 1106 18-19 0.11636457292008373 1106 20-21 0.29300409719269993 1106 22-23 0.1563738691916967 1106 24-25 0.09282394557383356 1106 26-27 -0.27714722195724306 1106 28-29 0.006029986357141581 1106 30-31 0.17879107702158592 1106 32-33 0.6485047457201176 1106 34-35 0.4792040114830556 1106 36-37 0.6665384111083625 1106 38-39 0.9793092994018835 1106 40-41 0.668692237983322 1106 42-43 0.7611634525033395 1106 44-45 0.7579970405046268 1106 46-47 0.8850379780962854 1106 48-49 -0.039174680040360954 1106 50-51 0.8042907242421791 1106 52-53 1.2130848290090128 1106 54-55 1.4869935567340065 1106 56-57 1.744874197472619 1106 58-59 2.1661213820165663 1106 60-61 2.3855323605472343 1106 62-63 3.126251352663047 1106 64-65 3.0205831383401573 1106 66-67 3.428756507040916 1106 68-69 3.2032972261103545 1106 70-71 2.691263683762081 1106 72-73 2.9575994068468923 1106 74-75 2.7561051060343225 1106 76-77 3.140711966456145 1106 78-79 2.9847258111635426 1106 80-81 2.405114433859385 1106 82-83 3.5145312987869595 1106 84-85 3.5209158050782356 1106 86-87 2.828632453246467 1106 88-89 2.773244362798941 1106 90-91 2.44200599513702 1106 92-93 2.6736407463457965 1106 94-95 3.1653884360686746 1106 96-97 3.701692937061548 1106 98-99 3.947189357612224 1106 100-101 3.7472931065789474 1107 1 -0.11937831917787989 1107 2 -0.17554144894508283 1107 3 0.03160744849296293 1107 4 0.1213413038489577 1107 5 -0.034823992998461506 1107 6 0.1019472123207592 1107 7 -0.11525268991462667 1107 8 -0.42892114413442783 1107 9 -0.04473940694619927 1107 10-11 0.1978886546007672 1107 12-13 0.34471886633244964 1107 14-15 0.023865911080363844 1107 16-17 -0.518983603439942 1107 18-19 -0.5526495115869565 1107 20-21 -0.6013620999904035 1107 22-23 -0.22390782094914385 1107 24-25 -0.47759858963743795 1107 26-27 -1.2208091937882273 1107 28-29 -1.6207305770231386 1107 30-31 -1.4338849793164457 1107 32-33 -1.5768473669559384 1107 34-35 -1.133472044854976 1107 36-37 -1.255996800159238 1107 38-39 -1.3094230949643162 1107 40-41 -1.7608852268054136 1107 42-43 -2.02756894186286 1107 44-45 -2.3617212693545255 1107 46-47 -3.0093282190868074 1107 48-49 -3.426498623702333 1107 50-51 -2.7450050504057053 1107 52-53 -3.1319855935262026 1107 54-55 -2.7806120770688167 1107 56-57 -2.219914534921749 1107 58-59 -2.9254279137580816 1107 60-61 -1.6708056676217815 1107 62-63 -2.8103683656468093 1107 64-65 -2.951247847575331 1107 66-67 -1.9937787042266883 1107 68-69 -2.5995196752980974 1107 70-71 -2.2031025134210154 1107 72-73 -2.957893550899584 1107 74-75 -2.2016413728389175 1107 76-77 -1.9015415546706151 1107 78-79 -2.487105174751953 1107 80-81 -1.8906602140279354 1107 82-83 -1.2812433491003645 1107 84-85 -1.4861264484428922 1107 86-87 -1.3403816312605734 1107 88-89 -1.1352063414264144 1107 90-91 -0.8396841457080484 1107 92-93 -1.2770634790063156 1107 94-95 -1.4472876202693534 1107 96-97 -1.713800020684932 1107 98-99 -1.7711205015427076 1107 100-101 -0.34425618919570056 1108 1 0.07780477941366826 1108 2 0.4300923538718209 1108 3 0.25695956116901897 1108 4 0.13542581089121342 1108 5 0.049683049255065725 1108 6 -0.13748940739754545 1108 7 -0.3969428307596985 1108 8 -0.10497748216259595 1108 9 -0.016570392861694927 1108 10-11 0.3598604855866867 1108 12-13 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-1.1261420093545738 2316 84-85 -1.563161831472776 2316 86-87 -1.2688426412227862 2316 88-89 -1.0905481483656096 2316 90-91 -1.3591791646019011 2316 92-93 -1.9005605590475412 2316 94-95 -1.6608740166966989 2316 96-97 -1.1390834284485827 2316 98-99 -1.5442225283376239 2316 100-101 -0.8346375014595253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 3.0 6 22.0 7 41.0 8 70.0 9 153.0 10 203.0 11 370.0 12 489.0 13 634.0 14 694.0 15 936.0 16 1129.0 17 1418.0 18 1493.0 19 1210.0 20 1259.0 21 1341.0 22 1412.0 23 1441.0 24 1484.0 25 1677.0 26 1367.0 27 1165.0 28 1284.0 29 1540.0 30 1900.0 31 2264.0 32 2649.0 33 3254.0 34 4147.0 35 5813.0 36 8178.0 37 12096.0 38 9608.0 39 820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.412269587607796 21.61414694622689 16.36762441928584 22.60595904687947 2 14.195810849383589 22.806540620624975 39.897513966100774 23.100134563890666 3 19.333704856533146 27.096274347229205 34.63049299316307 18.939527803074583 4 11.645893082872327 21.269250112136575 34.81398920770412 32.27086759728697 5 11.164738351936698 39.299552703492715 34.792598534390166 14.743110410180412 6 25.970883684260603 37.52361792651597 17.934671795777998 18.57082659344543 7 21.87682646695029 36.924875290535674 20.14516589417026 21.053132348343777 8 23.210232292615295 33.324271791874516 21.634883309999864 21.830612605510325 9 21.501678650555245 18.553506136928952 22.06711883758546 37.877696374930345 10-11 22.15275040437129 26.393551807097904 28.06948389990621 23.384213888624593 12-13 26.036753612156964 24.36218075056748 26.80336001957293 22.797705617702626 14-15 23.007027225401313 27.091517037963325 26.32966793981324 23.571787796822115 16-17 20.75616602214263 30.677531824082315 27.381963748071524 21.184338405703528 18-19 19.410501420396624 28.180261244240256 29.421239347025324 22.987997988337796 20-21 19.667396120754102 26.35005640809558 29.019586521863232 24.962960949287083 22-23 19.255549061450843 28.427641326065977 29.3838604885077 22.93294912397548 24-25 20.758858789468675 29.120169632056108 30.361147734841175 19.75982384363404 26-27 20.433461781556467 27.390734057808498 31.863994399929318 20.311809760705717 28-29 16.855146729010073 26.295007543733263 32.99126014326297 23.858585583993694 30-31 17.600005436924874 26.609669570890702 33.13669788367699 22.65362710850743 32-33 18.17020293322097 27.748025716654663 32.49582036400212 21.58595098612225 34-35 18.89162260252559 28.50802669675262 32.91625321135835 19.684097489363435 36-37 20.034257050223285 27.15007170870632 31.030498290546007 21.78517295052439 38-39 20.682014368929398 25.158540812789298 32.554393262780124 21.605051555501177 40-41 18.688533503700732 26.247001012689196 33.95975070174604 21.10471478186403 42-43 20.114723792953455 26.559101783384083 33.07824053936494 20.24793388429752 44-45 18.394546541974773 27.332535885167463 32.0227544584602 22.250163114397566 46-47 21.611776379279878 25.528401136317296 29.350559323646547 23.509263160756277 48-49 20.648362104118526 25.478455892347423 30.939921163517738 22.933260840016313 50-51 19.08836362153566 27.18054668279621 32.01737097497656 21.713718720691578 52-53 18.730885810985306 28.147639694988513 33.01436707398295 20.107107420043224 54-55 17.500781557950823 28.2849220480896 31.154938766633595 23.059357627325987 56-57 20.7195818976356 29.323573986856143 28.17433618094209 21.782507934566166 58-59 18.680721212986185 30.427956857707912 30.353878252832317 20.537443676473586 60-61 19.47315839908659 32.19700831175539 27.942585683120274 20.387247606037747 62-63 18.549428443272483 33.360291419173315 27.378314825134904 20.711965312419295 64-65 18.177925784966696 33.367541117303254 27.787821122740247 20.666711974989806 66-67 18.12621062289734 32.02501104427906 27.671186325483397 22.177592007340195 68-69 17.394081907273247 31.555232360576856 28.291718204183713 22.75896752796618 70-71 18.859333161164045 30.053961479387258 27.399382909026652 23.68732245042204 72-73 18.475350341846653 28.58667137866823 28.267931657854316 24.670046621630807 74-75 17.877968221174104 29.4796862894347 28.27472781394843 24.36761767544277 76-77 18.778432852567374 28.190429561326564 28.495753347341505 24.53538423876456 78-79 18.002327623170057 27.614322369003137 29.611858627519037 24.771491380307765 80-81 18.09352933580715 28.29191048042354 28.67861439027871 24.935945793490596 82-83 17.773987032934173 27.77385110981229 28.55133136697884 25.9008304902747 84-85 17.98534748746109 26.999768930692802 28.651914477171715 26.362969104674395 86-87 18.334331120060956 26.934118863611623 28.609720256884728 26.12182975944269 88-89 18.44733036859193 26.231822770305296 28.324420920524922 26.99642594057785 90-91 17.877884162611835 25.921123569434872 29.06785502241816 27.13313724553513 92-93 17.85906035707247 26.610530035815987 28.59773958312095 26.932670023990596 94-95 17.56126734718843 26.112870560411032 28.69676910739286 27.62909298500768 96-97 17.208546845903957 25.734324665968927 28.518030202117682 28.539098286009434 98-99 17.42194614725911 25.656168870886624 28.178902013021435 28.74298296883283 100-101 17.647018845868928 24.929829211436648 28.976967670465744 28.44618427222868 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 276.0 1 184.0 2 92.0 3 89.0 4 72.5 5 75.5 6 122.0 7 168.5 8 206.0 9 241.5 10 223.5 11 190.5 12 173.5 13 173.0 14 187.5 15 178.5 16 192.0 17 211.0 18 210.0 19 227.5 20 251.5 21 282.0 22 315.0 23 344.0 24 389.5 25 476.5 26 554.5 27 639.5 28 731.0 29 823.5 30 919.0 31 1004.0 32 1128.5 33 1246.0 34 1368.0 35 1501.5 36 1622.5 37 1745.5 38 1801.0 39 1801.5 40 1913.0 41 2063.5 42 2232.0 43 2482.5 44 2812.5 45 3213.5 46 3567.0 47 3975.0 48 4505.0 49 4371.0 50 3571.5 51 2817.5 52 2158.0 53 1738.5 54 1532.5 55 1348.0 56 1187.5 57 1006.0 58 848.5 59 741.0 60 626.0 61 493.0 62 386.0 63 320.5 64 242.5 65 196.0 66 165.0 67 153.5 68 122.0 69 84.0 70 79.0 71 67.5 72 51.5 73 44.0 74 33.5 75 27.0 76 25.0 77 16.5 78 11.0 79 11.5 80 11.5 81 6.5 82 2.5 83 2.0 84 2.0 85 2.0 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23106930719984778 2 0.0 3 0.0 4 0.0 5 0.02446616193880741 6 0.005436924875290536 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003398078047056585 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.79615609411317E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005436924875290536 36-37 0.012912696578815022 38-39 0.012912696578815022 40-41 0.006116540484701853 42-43 0.004077693656467902 44-45 0.004077693656467902 46-47 0.0 48-49 0.001359231218822634 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.79615609411317E-4 58-59 6.79615609411317E-4 60-61 6.79615609411317E-4 62-63 0.0 64-65 0.001359231218822634 66-67 0.004757309265879218 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.057767326799961945 78-79 0.1433988935857879 80-81 6.79615609411317E-4 82-83 0.0 84-85 0.0 86-87 0.10194234141169756 88-89 0.360196272987998 90-91 0.413885906131492 92-93 6.79615609411317E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.79615609411317E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 73571.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.54506531106006 #Duplication Level Percentage of deduplicated Percentage of total 1 90.70262875012214 63.079202403120796 2 6.529854392651227 9.08238300417284 3 1.303625525261409 2.7198216688640904 4 0.5081598749144923 1.4136004675755394 5 0.244307632170429 0.8495195117641461 6 0.14267565718753053 0.5953432738443136 7 0.09185966969608131 0.4471870709926466 8 0.06254275383562982 0.3479631920185943 9 0.04495260431935894 0.2813608622962852 >10 0.2677611648587902 3.4470103709341995 >50 0.03518029903254178 1.82680675809762 >100 0.0547249096061761 7.289557026545785 >500 0.007817844229453728 3.6128365796305606 >1k 0.003908922114726864 5.007407810142583 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2361 3.2091449076402387 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1323 1.7982629025023444 RNA PCR Primer, Index 39 (95% over 23bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 921 1.2518519525356457 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 679 0.9229179975805685 RNA PCR Primer, Index 39 (96% over 25bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 547 0.7434994766959807 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 511 0.694567152818366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.6782563781924943 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 342 0.4648570768373408 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 324 0.4403909148985334 RNA PCR Primer, Index 39 (96% over 28bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 310 0.42136167783501655 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 309 0.4200024466161939 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 266 0.3615555042068206 No Hit TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 213 0.28951624960922107 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 209 0.2840793247339305 No Hit CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 197 0.2677685501080589 RNA PCR Primer, Index 39 (96% over 30bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 196 0.26640931888923625 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 193 0.2623316252327684 Illumina PCR Primer Index 11 (96% over 25bp) CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 170 0.23106930719984778 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 165 0.22427315110573462 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 154 0.20932160769868563 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 152 0.20660314526104034 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 152 0.20660314526104034 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.20660314526104034 No Hit TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT 148 0.20116622038574983 RNA PCR Primer, Index 39 (95% over 22bp) TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 137 0.18621467697870084 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 137 0.18621467697870084 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 134 0.18213698332223294 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 131 0.17805928966576504 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 130 0.17670005844694242 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 116 0.15767082138342553 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 110 0.14951543407048973 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 108 0.14679697163284447 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 107 0.14543774041402183 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 102 0.13864158431990864 No Hit ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC 98 0.13320465944461812 Illumina PCR Primer Index 11 (96% over 26bp) GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA 97 0.13184542822579548 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 96 0.13048619700697286 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 91 0.12369004091285969 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 86 0.11689388481874652 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 84 0.11417542238110125 No Hit CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 84 0.11417542238110125 RNA PCR Primer, Index 39 (95% over 21bp) TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 80 0.10873849750581072 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 75 0.10194234141169756 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0027184624376452677 0.0 22-23 0.0 0.0 0.0 0.012912696578815022 0.0 24-25 0.0 0.0 0.0 0.01767000584469424 0.0 26-27 0.0 0.0 0.0 0.03126231803292058 0.0 28-29 0.0 0.0 0.0 0.0761169482540675 0.0 30-31 0.0 0.0 0.0 0.1610688994304821 0.0 32-33 0.0 0.0 0.0 0.3486428076280056 0.0 34-35 0.0 0.0 0.0 0.6694213752701472 0.0 36-37 0.0 0.0 0.0 1.0982588248086882 0.0 38-39 0.0 0.0 0.0 1.533212814831931 0.0 40-41 0.0 0.0 0.0 1.936224871212842 0.0 42-43 0.0 0.0 0.0 2.3494311617349224 0.0 44-45 0.0 0.0 0.0 2.877492490247516 0.0 46-47 0.0 0.0 0.0 3.458563836294192 0.0 48-49 0.0 0.0 0.0 3.985945549197374 0.0 50-51 0.0 0.0 0.0 4.511288415272322 0.0 52-53 0.0 0.0 0.0 4.987019341860243 0.0 54-55 0.0 0.0 0.0 5.557216838156339 0.0 56-57 0.0 0.0 0.0 6.23411398513001 0.0 58-59 0.0 0.0 0.0 6.90421497600957 0.0 60-61 0.0 0.0 0.0 7.602859822484403 0.0 62-63 0.0 0.0 0.0 8.299465822131001 0.0 64-65 0.0 0.0 0.0 9.020537983716409 0.0 66-67 0.0 0.0 0.0 9.875494420355846 0.0 68-69 0.0 0.0 0.0 10.831713582797569 0.0 70-71 0.0 0.0 0.0 11.75055388672167 0.0 72-73 0.0 0.0 0.0 12.659199956504601 0.0 74-75 0.0 0.0 0.0 13.639205665275721 0.0 76-77 0.0 0.0 0.0 14.592706365279797 0.0 78-79 0.0 0.0 0.0 15.701839039839067 0.0 80-81 0.0 0.0 0.0 16.788544399287765 0.0 82-83 0.0 0.0 0.0 17.80456973535768 0.0 84-85 0.0 0.0 0.0 19.031955525954523 0.0 86-87 0.0 0.0 0.0 20.234875154612553 0.0 88-89 0.0 0.0 0.0 21.34808552282829 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTATC 20 0.0020377361 71.50052 1 GTACCCA 30 1.2708751E-4 63.55601 1 GTACACT 25 0.005027674 56.92821 4 ACCCATG 35 2.7880774E-4 54.217342 3 TACCCAT 40 5.3885265E-4 47.440178 2 AGTACAC 40 5.3885265E-4 47.440178 3 CTTTACA 45 9.402215E-4 42.370674 1 TACACTG 45 9.6258544E-4 42.169044 5 CATACAC 55 0.0025799177 34.50195 3 AACAGCT 60 0.003951465 31.626783 7 TAGTTTT 70 0.00839293 27.108671 7 TTACACA 70 0.00839293 27.108671 3 GAGTACT 460 0.0 24.235743 12-13 GTACTTT 510 0.0 22.32479 14-15 ACTTTTT 605 0.0 19.211311 16-17 CATGGGA 170 0.0017811464 16.743591 4 GTACATG 315 2.0341758E-8 16.645622 1 TACATGG 315 1.3089993E-7 16.56641 2 AGTACTT 490 0.0 16.458838 12-13 CTTGAAA 865 0.0 15.356358 56-57 >>END_MODULE