FastQCFastQC Report
Thu 26 May 2016
SRR938855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304457
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53561.7591975221459846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53311.7509861819567294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47641.564752986464427No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40561.3322078323047262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.9925868020771407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.3908597930085365No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.360313607504508No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.30710412307813584No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.22334845314773513No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.15437319555799342No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.13827896878705367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.048.4206161
GTATCAA36800.033.892911
CATAAGC650.005892534329.1745853
CATAAGA650.005892534329.1745852
TATCAAC46350.027.2075842
ATGGGCG700.00846105927.0906895
GACCGAG700.00849506727.0684247
ATCAACG46600.026.9598853
TCAACGC46900.026.6863464
CAACGCA47550.026.5209565
AACGCAG48850.025.7897246
GTTCTAG950.001235915724.960161
ATGGGAT7050.024.8811635
GAGTACT32950.023.86457412-13
CCTTAAT1000.001666252223.7121521
GAGCTTC1201.7388641E-423.6848729
GTACATG27100.023.6246551
ACGCAGA54750.023.0143387
TACATGG27750.022.892852
CGCAGAG55300.022.7854468
Produced by FastQC (version 0.11.5)