FastQCFastQC Report
Thu 26 May 2016
SRR938855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304457
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55071.8087940168890846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50511.659019171837074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47911.5736212338688222No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36481.198198760416085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31511.0349573174536961No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT19800.6503381429889935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.40728247338704643No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT10180.3343657725064623TruSeq Adapter, Index 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.307432576685706No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.2893676282693451No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT5970.19608680371940865RNA PCR Primer, Index 1 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.19444453568155767No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.15043175226715103No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.12152783480097354No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG156.5575226E-495.202271
GGTATCA22650.053.5906831
GTATACA608.317141E-539.5503464
GTATCAA35500.034.996891
TCTATAC550.002587215334.5166663
GCTAAGA600.00390553731.7340891
TAGATAG600.00396256831.6402785
CGTCAGA600.00396256831.6402782
GGATATG650.005777032629.2930071
CTAGAGC650.005861241429.2064113
ATCAACG45700.027.5208133
TATCAAC45950.027.2677942
TCAACGC46850.026.8452744
CAACGCA47350.026.5617985
AACGCAG48650.025.7544736
TACCTGG1151.2859881E-424.761962
GTGGTAT9200.023.8005681
TACACTG2202.1736923E-923.730215
GAGTACT35750.023.16599512-13
ACGCAGA54850.022.7567757
Produced by FastQC (version 0.11.5)