FastQCFastQC Report
Thu 26 May 2016
SRR938854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303236
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55061.8157474706169452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54471.7962906778878498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46861.545331029297313No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40231.3266894432059517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.9896582200002638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.37792346555158357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.3390098800933926No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.33802055164954026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.21435449616800115No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.16060098405202547No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.12927224999670225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.051.4215661
GTATCAA36200.038.7020231
ATAGTAC600.00396889531.6298983
TATCAAC45250.031.0357462
ATCAACG46000.030.5297283
TCAACGC46750.030.0399484
CAACGCA47450.029.6967775
GTCACTA1303.0288356E-729.2258281
AACGCAG48250.029.1060626
TCACTAC1153.887193E-628.8794732
CACTACC1205.4131706E-627.676163
ACGCAGA52500.026.6638957
CGCAGAG53050.026.3874578
GAGTACT33150.026.14565712-13
GCAGAGT53450.025.4797449
GTGGTAT9200.025.2946361
TGGTATC9600.025.2050762
GTACTTT35700.024.4111414-15
GTACATG25550.023.234821
CAGAGTA52750.022.82315610-11
Produced by FastQC (version 0.11.5)