##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938854_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303236 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.316166286324844 33.0 31.0 34.0 30.0 34.0 2 31.31444155707106 33.0 31.0 34.0 28.0 34.0 3 31.260157105356885 33.0 31.0 34.0 28.0 34.0 4 34.8784840850031 37.0 35.0 37.0 32.0 37.0 5 34.90911369362477 37.0 35.0 37.0 32.0 37.0 6 35.01252819586065 37.0 35.0 37.0 32.0 37.0 7 34.93776134759725 37.0 35.0 37.0 32.0 37.0 8 34.922687279874424 37.0 35.0 37.0 32.0 37.0 9 36.434618580907284 39.0 37.0 39.0 32.0 39.0 10-11 36.328150351541375 39.0 37.0 39.0 32.0 39.0 12-13 36.198455988075295 39.0 37.0 39.0 32.0 39.0 14-15 37.219137899194024 40.0 37.0 41.0 32.0 41.0 16-17 37.09197786542495 40.0 37.0 41.0 31.5 41.0 18-19 37.05689462992521 40.0 36.5 41.0 32.0 41.0 20-21 36.99804442744265 40.0 36.0 41.0 31.5 41.0 22-23 36.863846970676306 40.0 36.0 41.0 31.0 41.0 24-25 36.733380931024016 40.0 36.0 41.0 31.0 41.0 26-27 36.52685861836985 39.0 35.0 41.0 30.0 41.0 28-29 36.26771392578718 39.0 35.0 41.0 30.0 41.0 30-31 35.822563943595085 38.5 35.0 41.0 29.5 41.0 32-33 35.40110343099104 38.0 35.0 40.0 28.5 41.0 34-35 34.97326010104341 38.0 35.0 40.0 26.0 41.0 36-37 34.64216649738158 38.0 35.0 40.0 24.5 41.0 38-39 34.35039869936287 38.0 35.0 40.0 24.0 41.0 40-41 34.00715779129127 38.0 34.0 40.0 22.0 41.0 42-43 33.67100047487766 38.0 33.5 40.0 17.5 41.0 44-45 33.21877019878906 38.0 33.0 40.0 11.5 41.0 46-47 32.5277688005382 37.0 33.0 40.0 2.0 41.0 48-49 31.89846192404596 37.0 32.0 40.0 2.0 41.0 50-51 30.622752575551715 36.0 29.5 39.0 2.0 40.0 52-53 30.634204052289306 35.5 30.5 39.0 2.0 40.0 54-55 31.001554894537588 36.0 31.0 40.0 2.0 41.0 56-57 30.80889142450105 35.5 31.0 40.0 2.0 41.0 58-59 30.548543048978352 35.0 30.5 40.0 2.0 41.0 60-61 30.21528941154744 35.0 29.5 39.5 2.0 41.0 62-63 29.76835863815642 35.0 28.5 39.0 2.0 41.0 64-65 29.33050000659552 35.0 28.0 39.0 2.0 41.0 66-67 28.905276748143358 35.0 27.0 38.0 2.0 40.0 68-69 28.430781635425873 35.0 26.0 37.5 2.0 40.0 70-71 27.95718516271155 35.0 25.5 37.0 2.0 39.5 72-73 27.48326880713372 34.5 24.5 36.5 2.0 39.0 74-75 26.962121911646374 34.0 22.5 36.0 2.0 39.0 76-77 26.439418472740705 34.0 20.0 35.0 2.0 38.5 78-79 25.93883476895883 34.0 16.5 35.0 2.0 37.0 80-81 25.40333106887045 33.0 7.5 35.0 2.0 37.0 82-83 24.93187484335633 33.0 2.0 35.0 2.0 36.0 84-85 24.536232505375352 33.0 2.0 35.0 2.0 36.0 86-87 24.002883892413827 33.0 2.0 35.0 2.0 35.5 88-89 23.46377573902835 32.0 2.0 35.0 2.0 35.0 90-91 22.9340843435476 32.0 2.0 35.0 2.0 35.0 92-93 22.385767191230592 31.0 2.0 35.0 2.0 35.0 94-95 21.798211623949662 31.0 2.0 35.0 2.0 35.0 96-97 21.15266656993233 30.5 2.0 34.5 2.0 35.0 98-99 20.419359838541595 29.5 2.0 34.0 2.0 35.0 100-101 18.46027021857563 25.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15676424784234655 1101 2 0.08433675245693095 1101 3 0.27266265474838036 1101 4 0.36957765511159835 1101 5 0.029875302036913354 1101 6 0.35140547149200074 1101 7 0.4987215686945632 1101 8 0.2357026845500414 1101 9 0.7262750799234965 1101 10-11 0.15932380886210495 1101 12-13 0.3359484560155863 1101 14-15 0.562073156544237 1101 16-17 0.6489805478170325 1101 18-19 0.5933042305319844 1101 20-21 0.9079364690939755 1101 22-23 0.8339805615841485 1101 24-25 0.6693726659886536 1101 26-27 0.46000023581329685 1101 28-29 0.6500401617633713 1101 30-31 0.7382300086838001 1101 32-33 0.7591001940514488 1101 34-35 0.8483094717651483 1101 36-37 0.6993996919571401 1101 38-39 0.4990913571929596 1101 40-41 0.7326753188614816 1101 42-43 1.0764625559599565 1101 44-45 1.1472781518474306 1101 46-47 1.3351315923176585 1101 48-49 1.3370278439541146 1101 50-51 0.990772266262173 1101 52-53 0.7893669062205255 1101 54-55 0.8266419297882202 1101 56-57 1.0480745242592704 1101 58-59 0.9081310924182411 1101 60-61 0.7434883864496129 1101 62-63 0.8868710024420281 1101 64-65 0.6063548320138317 1101 66-67 0.43405733004650315 1101 68-69 0.7341937069260602 1101 70-71 -0.009833690467257128 1101 72-73 0.028222974374887855 1101 74-75 -0.5762804765217631 1101 76-77 -0.28036211384120335 1101 78-79 -0.2649635776812289 1101 80-81 -0.6607045426306968 1101 82-83 -0.3427372079418305 1101 84-85 -0.4579547703867135 1101 86-87 -0.7356593454434517 1101 88-89 -0.6931938411296059 1101 90-91 -0.6849496581803081 1101 92-93 -0.910929323283657 1101 94-95 -1.7218197232371217 1101 96-97 -1.6719859534941186 1101 98-99 -2.0485864999269374 1101 100-101 -1.6823026707676831 1102 1 -0.0056918449521674574 1102 2 0.3395903280844479 1102 3 0.3527704086949832 1102 4 0.29383028332623695 1102 5 0.26147779955423545 1102 6 0.24030704328420427 1102 7 -0.12616000469569144 1102 8 0.18172297973193707 1102 9 0.18785919991390188 1102 10-11 0.19229491847662672 1102 12-13 0.37510057856287204 1102 14-15 0.590969249322221 1102 16-17 0.5177695715251858 1102 18-19 0.4813040478981776 1102 20-21 0.6350986821591462 1102 22-23 0.5294558089958841 1102 24-25 0.7408338505971912 1102 26-27 0.5126957966703145 1102 28-29 0.47652092243318833 1102 30-31 0.5539353746933671 1102 32-33 0.42873846496587475 1102 34-35 0.5608267363672894 1102 36-37 0.3757383406952499 1102 38-39 0.37396436962347224 1102 40-41 0.34078311846653975 1102 42-43 0.36767786980474426 1102 44-45 0.3625634600990537 1102 46-47 0.4427029051123057 1102 48-49 1.2742132286836458 1102 50-51 1.432181057797095 1102 52-53 1.3553376619820519 1102 54-55 0.9599132437243263 1102 56-57 1.2634968078667441 1102 58-59 1.1096674993263669 1102 60-61 0.9420569939809731 1102 62-63 0.8493112184065055 1102 64-65 0.8059792912465404 1102 66-67 0.621422494930588 1102 68-69 0.8820243597277795 1102 70-71 0.48788338693523414 1102 72-73 -0.015557773738848368 1102 74-75 0.17771178437063284 1102 76-77 0.47303087986934855 1102 78-79 0.5739933549839691 1102 80-81 0.21935070409616486 1102 82-83 0.023346552488273886 1102 84-85 0.21836675643191938 1102 86-87 0.45463366767193847 1102 88-89 0.6473040642889103 1102 90-91 0.9327806601732505 1102 92-93 1.339649968325464 1102 94-95 0.862351632933045 1102 96-97 0.47644396648944465 1102 98-99 0.7654694545059257 1102 100-101 0.9041344860805118 1103 1 -0.12826393117216384 1103 2 0.028091683067270168 1103 3 -0.13981970616674033 1103 4 -0.0045153903666843576 1103 5 0.20611059354015282 1103 6 0.08623585849630189 1103 7 0.09682821432396338 1103 8 0.10814571169478882 1103 9 0.2165916389963698 1103 10-11 -0.178063273216587 1103 12-13 0.02645612832306199 1103 14-15 0.1363073147015541 1103 16-17 -0.06370514869070121 1103 18-19 0.24481702511859993 1103 20-21 0.39015293816585483 1103 22-23 0.42559269060780736 1103 24-25 0.17996167253316742 1103 26-27 0.229169893253939 1103 28-29 -0.028984049616752827 1103 30-31 0.10810634064304026 1103 32-33 0.0401588724812143 1103 34-35 0.6165624523538185 1103 36-37 0.1820494991772179 1103 38-39 0.47989114385725173 1103 40-41 0.2324808722565237 1103 42-43 0.7937094142583874 1103 44-45 0.6925953220382084 1103 46-47 0.47519981529791977 1103 48-49 -0.17470561975104104 1103 50-51 -0.21613003265225927 1103 52-53 -0.3250621429911291 1103 54-55 -0.5183730148222736 1103 56-57 -0.3518354747407173 1103 58-59 -0.3361105102662343 1103 60-61 -0.5440870310550245 1103 62-63 -0.42209354160846146 1103 64-65 -0.3122387079143891 1103 66-67 -0.4224188530221973 1103 68-69 -0.6210824628091594 1103 70-71 -0.7494030190153502 1103 72-73 -0.6801626743545413 1103 74-75 -0.8901450750173012 1103 76-77 -1.050842896961015 1103 78-79 -1.037183269011134 1103 80-81 -1.576381383484481 1103 82-83 -1.7920866451577986 1103 84-85 -1.9171598726656285 1103 86-87 -1.5249792267754678 1103 88-89 -1.485847092832401 1103 90-91 -1.3095288201709963 1103 92-93 -1.2663221609818542 1103 94-95 -1.1561078819944441 1103 96-97 -1.173635971827654 1103 98-99 -0.49655814405631915 1103 100-101 -0.38864385233636867 1104 1 0.0736108520979677 1104 2 0.013393203252512365 1104 3 0.05096969392498352 1104 4 0.02480326685894596 1104 5 -0.08139346140576009 1104 6 -0.10039232813188903 1104 7 -0.2454875233406142 1104 8 -0.09630186484580605 1104 9 -0.20498850351400222 1104 10-11 0.1527699762213146 1104 12-13 0.008194889131587502 1104 14-15 0.06933079423665589 1104 16-17 0.15914349276258832 1104 18-19 0.21752863736509198 1104 20-21 0.2525588354554671 1104 22-23 0.36739722543559594 1104 24-25 0.05089693881727442 1104 26-27 -0.14949493889926657 1104 28-29 0.12777176992976536 1104 30-31 0.4424967564288096 1104 32-33 0.3160070553061374 1104 34-35 0.19933927931669615 1104 36-37 0.7876906280741593 1104 38-39 0.3434659841762766 1104 40-41 0.5072831586867181 1104 42-43 0.6509026086608785 1104 44-45 0.7915863396338807 1104 46-47 1.307655043101981 1104 48-49 1.2731617583489019 1104 50-51 1.522009949673766 1104 52-53 1.4053370874081033 1104 54-55 1.3143506783665337 1104 56-57 1.162124725265997 1104 58-59 0.7533052419402182 1104 60-61 0.9524452910586909 1104 62-63 0.8582458433820861 1104 64-65 1.1736836384730758 1104 66-67 1.3879271317423907 1104 68-69 0.6161402627856596 1104 70-71 1.0006389287010187 1104 72-73 0.8353747597875412 1104 74-75 0.652585665387857 1104 76-77 0.3203608347078344 1104 78-79 0.7331445943399864 1104 80-81 0.8902002691436799 1104 82-83 0.9409469018292391 1104 84-85 1.078141983101343 1104 86-87 0.742742285132092 1104 88-89 0.6505389156466279 1104 90-91 0.09282248915705793 1104 92-93 0.09234759303673812 1104 94-95 0.6492938652677687 1104 96-97 0.2892822469045164 1104 98-99 -0.030608503688075217 1104 100-101 0.03186802166387892 1105 1 -0.029718244608609723 1105 2 0.22541407421378068 1105 3 0.22717991980408314 1105 4 0.29373987354389897 1105 5 0.11951183883728334 1105 6 0.4334449408047192 1105 7 0.1992021994948061 1105 8 0.30990144864227176 1105 9 0.31693046542483216 1105 10-11 0.5415083533702827 1105 12-13 0.6133134031904959 1105 14-15 0.9429232307430055 1105 16-17 0.7159421637996672 1105 18-19 0.5516089548891117 1105 20-21 0.5146276471931586 1105 22-23 0.3815397544787871 1105 24-25 0.08586583305768869 1105 26-27 0.16374228990088113 1105 28-29 0.3178235013722315 1105 30-31 0.18938618047054945 1105 32-33 0.22564984077673955 1105 34-35 0.08462146095649103 1105 36-37 0.3117056123540465 1105 38-39 0.4389294488450375 1105 40-41 0.18519918428486193 1105 42-43 0.06184982921075033 1105 44-45 0.3946645834406013 1105 46-47 0.564405773362644 1105 48-49 0.6754461920902877 1105 50-51 0.6864133657328146 1105 52-53 0.42209963562759967 1105 54-55 0.35222389173643975 1105 56-57 0.22254682707882267 1105 58-59 0.24910763674923686 1105 60-61 0.34914964140542537 1105 62-63 0.2734687122473396 1105 64-65 0.038490648975450625 1105 66-67 0.6569083344984286 1105 68-69 0.6939381087954288 1105 70-71 0.6253797140378303 1105 72-73 0.7572424432446283 1105 74-75 0.9420643373168609 1105 76-77 0.8500283709978831 1105 78-79 0.6749132745192625 1105 80-81 0.5481428294041208 1105 82-83 0.2553380539687353 1105 84-85 0.3257170717360367 1105 86-87 0.23991043461812112 1105 88-89 0.21282290342730903 1105 90-91 0.834098382750831 1105 92-93 0.8325810534688749 1105 94-95 1.1876799334551613 1105 96-97 0.9108121151231288 1105 98-99 0.8661181446942017 1105 100-101 1.4487799667215207 1106 1 0.22993600842312745 1106 2 0.3110122508715598 1106 3 0.3012101941654848 1106 4 0.2438989859546652 1106 5 0.4623775823652778 1106 6 0.472599494859935 1106 7 0.03325225539916232 1106 8 0.02214450360055764 1106 9 0.14554059701509914 1106 10-11 0.27217834562968335 1106 12-13 0.3888466447833494 1106 14-15 0.27002824493411026 1106 16-17 0.32647153509063287 1106 18-19 0.2352655301019837 1106 20-21 0.4214501293467734 1106 22-23 0.5014761095144777 1106 24-25 0.12340776132809594 1106 26-27 -0.28321307261740003 1106 28-29 0.02994829710629432 1106 30-31 0.3364361503682076 1106 32-33 0.30031441461349573 1106 34-35 0.5531752581526774 1106 36-37 0.7391697045532339 1106 38-39 0.801017691727985 1106 40-41 0.964774741178303 1106 42-43 1.1325043402626136 1106 44-45 1.0503044733520142 1106 46-47 1.0858536963006387 1106 48-49 1.1344532446403797 1106 50-51 0.8065217341855515 1106 52-53 0.8096781167491294 1106 54-55 0.6908538048696649 1106 56-57 1.053178116319387 1106 58-59 0.9322374208723971 1106 60-61 0.7653361039495046 1106 62-63 0.5240707592070031 1106 64-65 0.7995855143749502 1106 66-67 0.9158852847005434 1106 68-69 1.0492210708664658 1106 70-71 0.8879044409665298 1106 72-73 0.8398841392969238 1106 74-75 0.6789805667827586 1106 76-77 0.6535137928607924 1106 78-79 0.8568819680773601 1106 80-81 0.4487620777054886 1106 82-83 0.13406859495093215 1106 84-85 0.2612227911821492 1106 86-87 0.2428024101922155 1106 88-89 0.3745047646124817 1106 90-91 0.1616656830002512 1106 92-93 0.10569535638450134 1106 94-95 -0.31757722660332277 1106 96-97 -0.25148254386027347 1106 98-99 -0.5186436167231854 1106 100-101 0.26984300963886554 1107 1 -0.40760036824368484 1107 2 -0.3236900033959138 1107 3 -0.19890925160613548 1107 4 -0.5267434151225174 1107 5 -0.418285233080141 1107 6 -0.5382266355314158 1107 7 -0.7454255884960688 1107 8 -0.4566050077576449 1107 9 -0.4476379635547687 1107 10-11 -0.5095725122081305 1107 12-13 -0.6657237847870832 1107 14-15 -0.6419402213821783 1107 16-17 -0.3510194521829675 1107 18-19 -0.15585964906667726 1107 20-21 -0.1489197181969999 1107 22-23 -0.29979870262989294 1107 24-25 -0.71189823702138 1107 26-27 -0.7022071220462323 1107 28-29 -0.8934380991061914 1107 30-31 -0.8587599578713849 1107 32-33 -0.6087927825371793 1107 34-35 -0.8707540733853563 1107 36-37 -1.18354345185557 1107 38-39 -0.9122798802652383 1107 40-41 -0.7372243466903541 1107 42-43 -0.9151762400266605 1107 44-45 -1.125297098988689 1107 46-47 -0.7936309494883567 1107 48-49 -0.19641697209939935 1107 50-51 0.030906045526386094 1107 52-53 -0.5032752342476989 1107 54-55 -0.4927560635228154 1107 56-57 -0.11747731520561189 1107 58-59 0.06866620947510071 1107 60-61 0.20837132959342597 1107 62-63 -0.31045341227368795 1107 64-65 -0.2715715893908488 1107 66-67 -0.4472135729635305 1107 68-69 -0.1136323800304595 1107 70-71 -0.1285627874572235 1107 72-73 -0.015255286494678444 1107 74-75 0.5123482860200461 1107 76-77 0.21796564535612006 1107 78-79 -0.0208164204905934 1107 80-81 0.3091951229211425 1107 82-83 0.36462065158994506 1107 84-85 0.25609205235576127 1107 86-87 0.2827353033859801 1107 88-89 0.5108195367968165 1107 90-91 0.5675587030237068 1107 92-93 0.7562947569839018 1107 94-95 0.7497521831173906 1107 96-97 0.7494187287801317 1107 98-99 1.2540890853573394 1107 100-101 1.493282613513255 1108 1 0.3993178633173464 1108 2 0.37210398599156136 1108 3 0.2523712239484226 1108 4 0.4254647699939511 1108 5 0.4525799594934554 1108 6 0.6430253587486945 1108 7 0.5367399337985006 1108 8 0.5613464677141806 1108 9 0.465913686822816 1108 10-11 0.4028859772207056 1108 12-13 0.5047029616113932 1108 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0.12379606674080179 2316 92-93 0.6547084901433955 2316 94-95 0.4234621013004123 2316 96-97 0.033076117817032014 2316 98-99 -0.33555057981659075 2316 100-101 -0.2592493669111384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3824.0 3 657.0 4 545.0 5 572.0 6 656.0 7 798.0 8 972.0 9 1177.0 10 1388.0 11 1871.0 12 2599.0 13 3285.0 14 3801.0 15 4733.0 16 5596.0 17 7002.0 18 6042.0 19 3881.0 20 3635.0 21 3481.0 22 3832.0 23 4224.0 24 4532.0 25 4884.0 26 5398.0 27 6038.0 28 6877.0 29 7446.0 30 8712.0 31 10055.0 32 11863.0 33 14149.0 34 18622.0 35 28611.0 36 31401.0 37 41077.0 38 33891.0 39 5109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45682432834538 20.760293855095846 16.275102326903877 18.5077794896549 2 16.28997839630557 23.398716991602967 39.32665182376142 20.98465278833004 3 18.406584329507233 29.12131845184994 32.23506399962994 20.23703321901288 4 12.46634756728009 19.85254040769406 36.738051115361365 30.94306090966448 5 11.923242206869194 38.540213546659615 33.69343162209514 15.843112624376054 6 26.57626401802766 37.04889540915789 19.322905308512254 17.051935264302188 7 23.463744400918994 37.73425232640783 22.637311779970577 16.164691492702598 8 24.909250798394616 32.91898360894202 22.054535476491164 20.117230116172202 9 26.78606814008481 17.35042339854948 22.728773927632535 33.13473453373317 10-11 23.14849237388533 28.78426629523333 26.96113663889178 21.106104691989565 12-13 25.484532470987535 27.995558280221623 28.17567266111562 18.34423658767522 14-15 22.745907054797236 27.99505146738197 29.554268687482654 19.704772790338136 16-17 18.571877376897383 31.2945203214392 30.054069248321703 20.07953305334171 18-19 17.990959648963333 27.756852569558642 35.73814383578624 18.514043945691785 20-21 19.792387771927245 26.758780893595013 36.01459200380661 17.43423933067113 22-23 19.43266772119992 27.293899078853634 35.75578464381404 17.51764855613241 24-25 18.62784632725037 27.74452219383286 36.159305081328355 17.46832639758841 26-27 18.075890981162914 28.041049159064663 36.109263689394915 17.773796170377505 28-29 17.931248935223547 27.992828162034577 36.37259815378101 17.70332474896086 30-31 18.003254377203483 28.414284863838713 35.890422738307066 17.692038020650745 32-33 17.718250526576632 28.704084582662205 35.50753727336555 18.070127617395613 34-35 18.14073478357278 28.60301101745119 35.34586324328081 17.910390955695217 36-37 18.256787491351144 28.49819408533981 34.87947878422713 18.365539639081913 38-39 18.035093061821648 28.645928152235438 35.13559585715184 18.183382928791072 40-41 17.94677082868029 28.692531802006144 35.073282344240894 18.28741502507267 42-43 17.88884696349843 29.05495756709166 34.286749811335746 18.76944565807417 44-45 17.887755895802886 29.19541675700636 34.048768538946476 18.868058808244275 46-47 18.143688986139573 29.27192195522423 33.41647825266566 19.167910805970546 48-49 18.381343853102873 29.573277224952776 32.586178069061646 19.4592008528827 50-51 18.36295857322108 29.909353056271954 32.084813491761324 19.64287487874564 52-53 18.43303631286303 29.9699889589827 31.846134236920037 19.750840491234225 54-55 18.19045553935257 29.907753322485974 31.798702620745818 20.10308851741564 56-57 18.403671334168532 30.083293476695864 31.24936905150029 20.263666137635315 58-59 18.29390159160848 29.53128619172099 31.769961285199035 20.404850931471493 60-61 18.356377348504896 29.35912278380524 31.973901825879864 20.310598041810003 62-63 18.515398466773473 29.276947316853573 32.14777496789228 20.059879248480676 64-65 18.436510774691726 29.190176289055774 32.1073561939577 20.265956742294804 66-67 18.390562913907285 29.37880794701987 31.66324503311258 20.567384105960265 68-69 18.55487118352209 29.20060931187496 31.554242002781642 20.690277501821313 70-71 18.477308815031535 29.323445212159143 31.303718642221217 20.895527330588102 72-73 18.608817160151258 29.0382182899451 31.18257494983477 21.170389600068873 74-75 18.574181500317863 29.174778819665182 31.096200201313838 21.154839478703117 76-77 18.62364060715409 28.893781139820902 31.217618724446726 21.264959528578288 78-79 18.56673804897908 28.83205518172701 31.15771739885943 21.44348937043448 80-81 18.740948790220475 28.892371909348046 30.821200229724877 21.5454790707066 82-83 18.77562437827003 28.35701079679092 30.86428948574966 22.003075339189387 84-85 19.00802780766366 27.842257717454277 30.86948605478772 22.280228420094346 86-87 19.33114851780128 27.75410894284721 30.583445056854856 22.331297482496648 88-89 19.678119899758002 27.450847322962634 30.442713419781576 22.428319357497788 90-91 19.784842630164746 27.275948101314025 30.426159268769563 22.513049999751665 92-93 20.073671837491517 27.0084267337715 30.381769117427943 22.53613231130904 94-95 20.12162714805365 26.572535046864367 30.380627076858886 22.925210728223096 96-97 20.55399644025001 26.144128482139163 30.211018667991222 23.090856409619605 98-99 20.806829206912653 25.976748113391857 30.075464325804564 23.140958353890934 100-101 21.74705406224085 25.17574656079664 30.516422599509713 22.560776777452794 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5178.0 1 3291.0 2 1464.5 3 1405.0 4 1115.0 5 1150.5 6 1681.0 7 2506.0 8 3380.0 9 3650.0 10 2938.5 11 2119.0 12 1897.5 13 1837.0 14 1778.5 15 1692.0 16 1578.0 17 1552.5 18 1590.0 19 1655.0 20 1761.0 21 1845.0 22 1943.0 23 2181.5 24 2338.0 25 2537.0 26 2831.0 27 3189.0 28 3482.0 29 3698.0 30 4080.0 31 4526.5 32 5016.0 33 5407.0 34 5854.0 35 6319.5 36 6852.0 37 7370.5 38 7500.5 39 7511.0 40 7488.5 41 7498.0 42 7423.0 43 7702.5 44 8207.0 45 8324.5 46 8397.5 47 8531.5 48 8907.0 49 8998.0 50 8560.5 51 8263.0 52 7957.5 53 7846.5 54 7711.0 55 7268.5 56 6824.0 57 6257.0 58 5782.0 59 5186.5 60 4518.0 61 3947.5 62 3309.5 63 2546.0 64 1860.5 65 1431.0 66 1113.0 67 903.5 68 720.0 69 532.0 70 402.0 71 340.0 72 296.0 73 241.0 74 213.5 75 175.0 76 122.5 77 82.0 78 57.5 79 41.0 80 35.5 81 28.0 82 17.5 83 13.5 84 9.0 85 6.0 86 7.5 87 5.5 88 4.0 89 4.0 90 2.0 91 0.5 92 0.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2552467385138968 2 0.1681858354548932 3 0.19061061351554565 4 0.16785605930694245 5 0.23941748341225977 6 0.1945679272909549 7 0.2410663641520136 8 0.24865121555488137 9 0.1477397142819454 10-11 0.2059452043952565 12-13 0.21451938424197656 14-15 0.1714835969344009 16-17 0.27899062116635226 18-19 0.2319975200833674 20-21 0.19934968143624107 22-23 0.24156102837393978 24-25 0.2298539751216874 26-27 0.33406323787413106 28-29 0.3108140194436017 30-31 0.2882243533089739 32-33 0.18912662084976717 34-35 0.35582846363888193 36-37 0.38682742154625444 38-39 0.3709981664446174 40-41 0.3345579020960572 42-43 0.3667110765212574 44-45 0.3744608159981005 46-47 0.38023189858723894 48-49 0.39622604176285137 50-51 0.39078473532166363 52-53 0.3893007426558852 54-55 0.38435410043662366 56-57 0.366546188447282 58-59 0.3383503277974911 60-61 0.30207495152290625 62-63 0.3719874948884697 64-65 0.4062842142753499 66-67 0.40760331886715295 68-69 0.4141988418261684 70-71 0.4146935060480946 72-73 0.4069437665712514 74-75 0.4023269004999407 76-77 0.3861678692503529 78-79 0.36506219578150356 80-81 0.3744608159981005 82-83 0.3810563389571159 84-85 0.3828701077708452 86-87 0.3795723462913374 88-89 0.3848487646585498 90-91 0.4038108931657191 92-93 0.4026566766478914 94-95 0.4113957445685868 96-97 0.4117255207165376 98-99 0.4085926473110053 100-101 0.3850136527325252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 303236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.63495283519418 #Duplication Level Percentage of deduplicated Percentage of total 1 84.61482544804304 52.998456010963636 2 8.969508739707571 11.236095137328913 3 2.6154906567977307 4.9146340178825065 4 1.1182678108651312 2.8017060636261335 5 0.6496252372020567 2.0344623046351322 6 0.4353518321438249 1.6360944883826307 7 0.302083971496092 1.3244710714848107 8 0.23585443201253148 1.1818184980061137 9 0.14324303297482285 0.8074818552913374 >10 0.8662109920598041 9.417064807809686 >50 0.03109290377815145 1.3223212308448997 >100 0.013701957597151475 1.5741762231401206 >500 5.269983691212106E-4 0.21455536696185332 >1k 0.003161990214727264 4.921239947745033 >5k 0.0010539967382424212 3.615422975897199 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5506 1.8157474706169452 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5447 1.7962906778878498 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4686 1.545331029297313 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4023 1.3266894432059517 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3001 0.9896582200002638 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1146 0.37792346555158357 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1028 0.3390098800933926 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1025 0.33802055164954026 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 650 0.21435449616800115 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 487 0.16060098405202547 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 392 0.12927224999670225 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2977614795077104E-4 0.0 12-13 0.0 0.0 0.0 3.2977614795077104E-4 0.0 14-15 0.0 0.0 0.0 3.2977614795077104E-4 0.0 16-17 0.0 0.0 0.0 3.2977614795077104E-4 0.0 18-19 0.0 0.0 0.0 6.595522959015421E-4 0.0 20-21 0.0 0.0 0.0 0.0018137688137292406 0.0 22-23 0.0 0.0 0.0 0.0062657468110646495 0.0 24-25 0.0 0.0 0.0 0.01467503858380931 0.0 26-27 0.0 0.0 0.0 0.02902030101966785 0.0 28-29 0.0 0.0 0.0 0.05523750478175414 0.0 30-31 0.0 0.0 0.0 0.11888430133625294 0.0 32-33 0.0 0.0 0.0 0.22935931089976125 0.0 34-35 0.0 0.0 0.0 0.40908731153293143 0.0 36-37 0.0 0.0 0.0 0.6768655436689575 0.0 38-39 0.0 0.0 0.0 0.97514806949043 0.0 40-41 0.0 0.0 0.0 1.2691435053885423 0.0 42-43 0.0 0.0 0.0 1.5623145009167776 0.0 44-45 0.0 0.0 0.0 1.938094421506681 0.0 46-47 0.0 0.0 0.0 2.379005131316862 0.0 48-49 0.0 0.0 0.0 2.819915841127043 0.0 50-51 0.0 0.0 0.0 3.2354337875450145 0.0 52-53 0.0 0.0 0.0 3.6417180018203643 0.0 54-55 0.0 0.0 0.0 4.103074832803493 0.0 56-57 0.0 0.0 0.0 4.681996860531072 0.0 58-59 0.0 0.0 0.0 5.241791871677505 0.0 60-61 0.0 0.0 0.0 5.827474310438074 0.0 62-63 0.0 0.0 0.0 6.382157791291272 0.0 64-65 0.0 0.0 0.0 6.981361052117823 0.0 66-67 0.0 0.0 0.0 7.665976335263623 0.0 68-69 0.0 0.0 0.0 8.393462517643023 0.0 70-71 0.0 0.0 0.0 9.176680869026104 0.0 72-73 0.0 0.0 0.0 9.960558772705088 0.0 74-75 0.0 0.0 0.0 10.743777124088169 0.0 76-77 0.0 0.0 0.0 11.577945890329644 0.0 78-79 0.0 0.0 0.0 12.488292946747748 0.0 80-81 0.0 0.0 0.0 13.402102652719334 0.0 82-83 0.0 0.0 0.0 14.307997731140102 0.0 84-85 0.0 0.0 0.0 15.23747180413935 0.0 86-87 0.0 0.0 0.0 16.207508343336542 0.0 88-89 0.0 0.0 0.0 17.213325594586394 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2115 0.0 51.421566 1 GTATCAA 3620 0.0 38.702023 1 ATAGTAC 60 0.003968895 31.629898 3 TATCAAC 4525 0.0 31.035746 2 ATCAACG 4600 0.0 30.529728 3 TCAACGC 4675 0.0 30.039948 4 CAACGCA 4745 0.0 29.696777 5 GTCACTA 130 3.0288356E-7 29.225828 1 AACGCAG 4825 0.0 29.106062 6 TCACTAC 115 3.887193E-6 28.879473 2 CACTACC 120 5.4131706E-6 27.67616 3 ACGCAGA 5250 0.0 26.663895 7 CGCAGAG 5305 0.0 26.387457 8 GAGTACT 3315 0.0 26.145657 12-13 GCAGAGT 5345 0.0 25.479744 9 GTGGTAT 920 0.0 25.294636 1 TGGTATC 960 0.0 25.205076 2 GTACTTT 3570 0.0 24.41114 14-15 GTACATG 2555 0.0 23.23482 1 CAGAGTA 5275 0.0 22.823156 10-11 >>END_MODULE