FastQCFastQC Report
Thu 26 May 2016
SRR938854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303236
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55301.8236620981677638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50341.6600931287841811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49201.6224986479177934No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37941.2511707053252252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31701.0453903890039442No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT18410.6071178883773695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.4138690656782176No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT10250.33802055164954026TruSeq Adapter, Index 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.3185637589204448No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.26909733672782915No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.19192971810734874No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT5300.17478135841390866RNA PCR Primer, Index 1 (95% over 23bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.16224986479177933No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.11674075637457294No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG3480.11476209948686832TruSeq Adapter, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.047.5414771
GTATCAA37850.033.9133381
TATCAAC47850.026.8764152
ATCAACG48550.026.3911633
TCAACGC48800.026.2559624
CAACGCA49650.025.8064655
AACGCAG50300.025.468786
ATATAGG1000.001659935723.727612
GAGTACT37450.023.50585712-13
GTATTAT1452.3113525E-522.9510571
GAAGTGT1050.00220668522.5939986
GTACATG26900.022.2684971
ACGCAGA57250.022.2111827
AAGTGTG1503.0497E-522.1421187
CGCAGAG57650.021.974778
GTACTTT40400.021.61326814-15
TACATGG27350.021.5153472
AGTACTT37450.021.3516812-13
ACATGGG26500.021.310083
CATGGGG6500.021.1723294
Produced by FastQC (version 0.11.5)