##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938854_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303236 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.849529739213022 31.0 31.0 34.0 30.0 34.0 2 32.04099447295176 33.0 31.0 34.0 30.0 34.0 3 32.049921513276786 33.0 31.0 34.0 30.0 34.0 4 35.7750728805287 37.0 35.0 37.0 35.0 37.0 5 35.69851205002045 37.0 35.0 37.0 33.0 37.0 6 35.74711116094395 37.0 35.0 37.0 33.0 37.0 7 35.64569839992613 37.0 35.0 37.0 33.0 37.0 8 35.666035694970255 37.0 35.0 37.0 33.0 37.0 9 37.220610349694624 39.0 37.0 39.0 33.0 39.0 10-11 37.17512927224999 39.0 37.0 39.0 33.0 39.0 12-13 37.085969344009285 39.0 37.0 39.0 33.0 39.0 14-15 38.14960459839861 40.0 37.0 41.0 33.0 41.0 16-17 38.05901673943727 40.0 37.0 41.0 33.0 41.0 18-19 38.149355617406904 40.0 37.0 41.0 33.0 41.0 20-21 38.15706743262673 40.0 37.0 41.0 33.0 41.0 22-23 38.00005276418367 40.0 37.0 41.0 33.0 41.0 24-25 37.85435766201903 40.0 36.5 41.0 33.0 41.0 26-27 37.568885290664696 40.0 36.0 41.0 32.5 41.0 28-29 37.2749195346199 39.5 35.5 41.0 32.0 41.0 30-31 36.83040107375113 39.0 35.0 41.0 31.0 41.0 32-33 36.371433470959914 38.0 35.0 41.0 30.5 41.0 34-35 36.093130433062036 38.0 35.0 41.0 30.0 41.0 36-37 35.78944947169862 38.0 35.0 41.0 30.0 41.0 38-39 35.48476763972616 38.0 35.0 40.5 28.5 41.0 40-41 35.149644171536366 38.0 35.0 40.0 26.5 41.0 42-43 34.72278192562888 38.0 34.5 40.0 24.5 41.0 44-45 34.19062050679999 38.0 33.5 40.0 22.5 41.0 46-47 33.48478247965281 38.0 33.0 40.0 17.5 41.0 48-49 32.80643459219881 37.0 33.0 40.0 5.0 41.0 50-51 32.400320212639656 37.0 33.0 40.0 2.0 41.0 52-53 32.084673323747836 36.5 32.0 40.0 2.0 41.0 54-55 31.713464100568537 36.0 31.5 40.0 2.0 41.0 56-57 31.33402531361712 35.5 31.0 40.0 2.0 41.0 58-59 30.918383701143664 35.0 31.0 39.0 2.0 41.0 60-61 30.40721418301257 35.0 29.5 39.0 2.0 41.0 62-63 29.927960400480153 35.0 29.0 39.0 2.0 40.0 64-65 29.406388093761954 35.0 28.0 38.0 2.0 40.0 66-67 28.890657771504703 35.0 27.5 37.5 2.0 40.0 68-69 28.52444630584759 35.0 26.5 37.0 2.0 40.0 70-71 28.090055270482395 34.0 26.0 36.5 2.0 39.0 72-73 27.593664670421717 34.0 25.5 36.0 2.0 39.0 74-75 27.083095344879894 34.0 24.5 35.5 2.0 38.5 76-77 25.288503014153992 31.5 20.0 34.5 2.0 36.0 78-79 26.093526164439577 33.0 21.0 35.0 2.0 37.0 80-81 26.082013679114617 33.0 20.0 35.0 2.0 36.5 82-83 25.73117802635571 33.0 19.0 35.0 2.0 36.0 84-85 25.315892572122046 33.0 14.5 35.0 2.0 36.0 86-87 24.70987613607883 33.0 4.5 35.0 2.0 35.0 88-89 24.0966046247807 32.0 2.0 35.0 2.0 35.0 90-91 23.5597356514398 31.5 2.0 35.0 2.0 35.0 92-93 23.214375931617617 31.0 2.0 34.0 2.0 35.0 94-95 22.8555761848857 31.0 2.0 34.0 2.0 35.0 96-97 22.274144560672216 31.0 2.0 34.0 2.0 35.0 98-99 21.488495759078738 31.0 2.0 34.0 2.0 35.0 100-101 18.5539909509425 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21561696136584274 1101 2 0.4258859745782999 1101 3 0.0478215634787702 1101 4 0.13521853876341794 1101 5 0.071013839130039 1101 6 0.09324540154538852 1101 7 0.2228890637926142 1101 8 0.3975432251016997 1101 9 0.17985449113108132 1101 10-11 0.37228882873078817 1101 12-13 0.18518012686062946 1101 14-15 0.6066634697167146 1101 16-17 0.2615360817901262 1101 18-19 0.21468347487299866 1101 20-21 0.29461686519245944 1101 22-23 0.12377725761897551 1101 24-25 0.11904507417981591 1101 26-27 0.024435986424435896 1101 28-29 -0.3812158000735195 1101 30-31 0.08179167706482104 1101 32-33 0.2374663763605227 1101 34-35 0.5041223221768902 1101 36-37 0.602622734472881 1101 38-39 0.7281007236572137 1101 40-41 0.9625774814238568 1101 42-43 0.9425807221331013 1101 44-45 0.8318849068293943 1101 46-47 0.9905796992792588 1101 48-49 0.5635548059556541 1101 50-51 0.7800863558212114 1101 52-53 1.0564805769187515 1101 54-55 0.9228576919302931 1101 56-57 0.9320326281199058 1101 58-59 1.0467734517585683 1101 60-61 1.52067957406366 1101 62-63 1.0268754245509193 1101 64-65 0.9596296113291309 1101 66-67 0.9278885918426099 1101 68-69 1.2006795631094782 1101 70-71 1.0597516069647703 1101 72-73 0.8033539478105851 1101 74-75 0.7648143261633074 1101 76-77 0.6608047406303896 1101 78-79 0.27867309118155603 1101 80-81 0.3874104199107329 1101 82-83 -0.05179259610854814 1101 84-85 0.09744567089625633 1101 86-87 0.1696517468439822 1101 88-89 0.13957111679714984 1101 90-91 0.20184310087721968 1101 92-93 -0.021656913075648987 1101 94-95 0.12849063004596672 1101 96-97 0.3572216894517055 1101 98-99 -0.12628536451349248 1101 100-101 0.15208761974640694 1102 1 0.4973980975764185 1102 2 0.5074890428262719 1102 3 0.33363205807394536 1102 4 0.2567841670807951 1102 5 0.0938658943311097 1102 6 0.10074480227820715 1102 7 0.29271225143743607 1102 8 0.09577510154992552 1102 9 0.20937268031687495 1102 10-11 0.20181729106827362 1102 12-13 0.5428798540513853 1102 14-15 0.4026557990967845 1102 16-17 0.35719053862576544 1102 18-19 0.16986399697739785 1102 20-21 0.23089478818948095 1102 22-23 0.26825772150885285 1102 24-25 0.3832077552441078 1102 26-27 0.30307810406343805 1102 28-29 0.5436931683879109 1102 30-31 0.36052511160772127 1102 32-33 0.20443819368106375 1102 34-35 0.503654323044401 1102 36-37 0.4017654970375162 1102 38-39 0.2195854223685032 1102 40-41 0.053203801238481674 1102 42-43 0.4907161451015867 1102 44-45 0.3254356504914355 1102 46-47 0.06474043985934941 1102 48-49 -0.10399459078204387 1102 50-51 0.13617662258071306 1102 52-53 -0.40227842635724187 1102 54-55 -0.12972372279683597 1102 56-57 -0.06306479560194589 1102 58-59 -0.11827614473486392 1102 60-61 -0.30270211918003653 1102 62-63 -0.16159125048646317 1102 64-65 0.06057474128450124 1102 66-67 0.2779119917992361 1102 68-69 0.3986489034589944 1102 70-71 0.44389328514117565 1102 72-73 0.04085209293597103 1102 74-75 0.09271620615006526 1102 76-77 0.22049175194227288 1102 78-79 -0.33389782210162977 1102 80-81 -0.05471178493591111 1102 82-83 0.30797888333944456 1102 84-85 0.2300321080531056 1102 86-87 0.34803931861336324 1102 88-89 0.04514943816416306 1102 90-91 0.31441972146690134 1102 92-93 0.507946885707554 1102 94-95 0.3739162238912108 1102 96-97 0.5886986262261651 1102 98-99 0.9655509042778156 1102 100-101 1.9156224970963898 1103 1 0.16183858605056045 1103 2 0.09708232120437543 1103 3 0.28952424906416496 1103 4 0.17883013444781426 1103 5 -6.314013726296253E-4 1103 6 0.07420074549365552 1103 7 0.30322328972845725 1103 8 -0.015061359393683915 1103 9 -0.09218186482843294 1103 10-11 0.18729232876967927 1103 12-13 0.06690103487072463 1103 14-15 -0.006543343692321457 1103 16-17 -0.031406062011470226 1103 18-19 0.1838220208568515 1103 20-21 0.40942369637947706 1103 22-23 0.330496221163024 1103 24-25 0.05271100380124949 1103 26-27 0.3111002826943974 1103 28-29 0.16252913042570327 1103 30-31 0.3379723012939806 1103 32-33 0.15017651758432038 1103 34-35 0.39519333407702106 1103 36-37 0.29071926887881716 1103 38-39 0.5852380251827896 1103 40-41 0.4431383765449155 1103 42-43 0.4297305793537447 1103 44-45 0.5875766367375093 1103 46-47 0.3430391710511671 1103 48-49 0.027665518296906555 1103 50-51 0.17561852840090353 1103 52-53 0.21641013169158185 1103 54-55 0.5179920823124071 1103 56-57 0.3285592561935502 1103 58-59 0.544956764906491 1103 60-61 0.32328293632323835 1103 62-63 0.3327677122430046 1103 64-65 0.40417343959388674 1103 66-67 0.05850115420441426 1103 68-69 0.0727670307480075 1103 70-71 0.04861814992635871 1103 72-73 0.5037006183291446 1103 74-75 0.6019402942296175 1103 76-77 0.33579001824458743 1103 78-79 0.11697129449492394 1103 80-81 0.41458849966717537 1103 82-83 0.5800866469941539 1103 84-85 0.7227526187512332 1103 86-87 0.7651472523495997 1103 88-89 0.17131591396156054 1103 90-91 0.9294345185596384 1103 92-93 1.1215613449049968 1103 94-95 1.110318205907923 1103 96-97 0.30481555160573137 1103 98-99 -0.044234452836693805 1103 100-101 0.9226128478044977 1104 1 -0.029543675099141353 1104 2 -0.07926587839963872 1104 3 -0.08265826265323284 1104 4 0.0708175613189681 1104 5 -0.08533223025951031 1104 6 -0.1130426743079056 1104 7 -0.0336112796642567 1104 8 -0.2819383239450701 1104 9 -0.06969788885608352 1104 10-11 -0.1355093312412805 1104 12-13 -0.06531816711591887 1104 14-15 -0.15907642645796471 1104 16-17 0.07862559453181461 1104 18-19 -0.05538622997930531 1104 20-21 0.01664765244063915 1104 22-23 -0.18000189398626532 1104 24-25 -0.2379698915308026 1104 26-27 -0.3940352363946147 1104 28-29 -0.18312564546162236 1104 30-31 -0.5785125919350946 1104 32-33 -0.4653139351154394 1104 34-35 -0.34844459560741825 1104 36-37 -0.21426971285434604 1104 38-39 -0.28367815768688587 1104 40-41 0.020998931149655675 1104 42-43 0.4151998904044518 1104 44-45 0.1838606283703328 1104 46-47 -0.063596398375104 1104 48-49 0.022486373582871977 1104 50-51 -0.21504894366191962 1104 52-53 -0.22008102572981159 1104 54-55 -0.37726167340645844 1104 56-57 -0.43111388720052446 1104 58-59 -0.5439779868189589 1104 60-61 -0.25737972573477563 1104 62-63 0.16972532827038833 1104 64-65 -0.03467238297286457 1104 66-67 -0.15902851681362762 1104 68-69 -0.2673282744635763 1104 70-71 0.07103500955252073 1104 72-73 0.11041001573621756 1104 74-75 0.03089204876276952 1104 76-77 0.08783986331768645 1104 78-79 0.051980299271374264 1104 80-81 0.05515250069629474 1104 82-83 0.21017490498938685 1104 84-85 0.07819519512404227 1104 86-87 0.5105805442945197 1104 88-89 0.4440991169773234 1104 90-91 0.50822351595329 1104 92-93 0.42242155274194815 1104 94-95 0.48024113397038803 1104 96-97 0.8732965968309614 1104 98-99 0.9218717923393172 1104 100-101 0.926740503094944 1105 1 0.2577330658199877 1105 2 0.2952407775639685 1105 3 0.34460554540527966 1105 4 0.21200872237767499 1105 5 0.09304090506726936 1105 6 0.33960744721638747 1105 7 0.34299139164040326 1105 8 0.3270433247716795 1105 9 0.1854027867743966 1105 10-11 0.4226996905439009 1105 12-13 0.30801784198168036 1105 14-15 0.5955281178816776 1105 16-17 0.6687765522324369 1105 18-19 0.3895710692826242 1105 20-21 0.3859588661227136 1105 22-23 0.5445839200035252 1105 24-25 0.5132509259151803 1105 26-27 0.5564854227353138 1105 28-29 0.6196147831066199 1105 30-31 0.6055186699107296 1105 32-33 0.8370975974285457 1105 34-35 1.029183785332222 1105 36-37 0.8598266592552619 1105 38-39 1.1080593052426337 1105 40-41 1.3118266342290141 1105 42-43 1.0748593994017028 1105 44-45 1.3283909202516213 1105 46-47 1.120167287044211 1105 48-49 1.251620986823447 1105 50-51 1.0378107793964801 1105 52-53 1.2947527424152199 1105 54-55 1.184292053710184 1105 56-57 0.9718729346604675 1105 58-59 1.1955475109846816 1105 60-61 1.522750494792799 1105 62-63 1.441148165918392 1105 64-65 1.2061909969633646 1105 66-67 1.3185229359033883 1105 68-69 1.2296447672114503 1105 70-71 1.3758881769884717 1105 72-73 1.278375542973265 1105 74-75 1.0585639108717153 1105 76-77 1.3073472997125393 1105 78-79 1.2124202395126353 1105 80-81 1.1747312908048855 1105 82-83 1.0377553451526111 1105 84-85 1.0223664886330823 1105 86-87 1.0268246631876465 1105 88-89 0.8592160701737477 1105 90-91 1.0983911971805718 1105 92-93 1.0707703832099824 1105 94-95 1.3405606648197192 1105 96-97 1.3376476125064798 1105 98-99 1.714098697149005 1105 100-101 2.4257370914955025 1106 1 0.1446987491432843 1106 2 0.14223484310108603 1106 3 -0.030469710464679167 1106 4 -0.08045708995568646 1106 5 -0.24406238898966848 1106 6 -0.09115715242238309 1106 7 -0.09914759520057004 1106 8 0.1057480637413164 1106 9 0.20916214000394717 1106 10-11 0.046549350817400637 1106 12-13 -0.21322581502356286 1106 14-15 0.04955753217598868 1106 16-17 0.11205512796134087 1106 18-19 0.022956723363648734 1106 20-21 -0.23539659960361092 1106 22-23 -0.1659927549771183 1106 24-25 0.2136895601286497 1106 26-27 0.2956593282999549 1106 28-29 0.1488248114888293 1106 30-31 -0.10977762320012374 1106 32-33 -0.07498204478354609 1106 34-35 0.27102389886107403 1106 36-37 0.32024207165743235 1106 38-39 0.21265421364548587 1106 40-41 0.12140778155850285 1106 42-43 0.26675111695567466 1106 44-45 0.029399348909059597 1106 46-47 -0.1688152535939551 1106 48-49 -0.008237532141031068 1106 50-51 0.25086014724716676 1106 52-53 -0.10139415704293953 1106 54-55 -0.23181915296393996 1106 56-57 -0.08389166497830303 1106 58-59 -0.3191703870113578 1106 60-61 -0.08848843184347999 1106 62-63 0.3461322546773147 1106 64-65 0.035553687253205624 1106 66-67 0.11105325326814253 1106 68-69 0.2517914946561923 1106 70-71 0.10819229670980945 1106 72-73 0.5220863024125038 1106 74-75 0.00876602663674575 1106 76-77 -0.0672226667448399 1106 78-79 0.046448793739866545 1106 80-81 -0.44274312219933165 1106 82-83 -0.19909637928189383 1106 84-85 -0.21918335225450392 1106 86-87 -0.18879415508018127 1106 88-89 0.22398127185947558 1106 90-91 -0.016427758697986405 1106 92-93 0.561911692232087 1106 94-95 0.39029404402329604 1106 96-97 0.543149891684255 1106 98-99 0.8165326870002119 1106 100-101 2.008991585346024 1107 1 -0.2325435737512116 1107 2 -0.21291001204377125 1107 3 -0.0826132191299287 1107 4 0.06498442505104407 1107 5 0.06098039431398661 1107 6 -0.14086831082919105 1107 7 0.005497759444786254 1107 8 -0.057306273226060966 1107 9 0.029352818890281185 1107 10-11 0.10473030030269825 1107 12-13 -0.028866695881845317 1107 14-15 -0.3080522493468365 1107 16-17 -0.22174151018312216 1107 18-19 0.007325615341230218 1107 20-21 0.08391452405533784 1107 22-23 -0.08859732432082268 1107 24-25 0.020382866821954337 1107 26-27 0.32878381251138933 1107 28-29 0.33450326347163184 1107 30-31 0.08179167706482104 1107 32-33 0.21071052351771868 1107 34-35 0.03589489742773111 1107 36-37 -0.11644081067762357 1107 38-39 -0.07624710242974686 1107 40-41 0.021105909517501686 1107 42-43 -0.14103131800067104 1107 44-45 0.20312236502338266 1107 46-47 0.3568004350652103 1107 48-49 0.5986718628118481 1107 50-51 0.4740662889315743 1107 52-53 0.2404270652130691 1107 54-55 0.4161687287195939 1107 56-57 0.5106279458456555 1107 58-59 0.2909206089492038 1107 60-61 0.08255248376265456 1107 62-63 -0.19051587979690865 1107 64-65 -0.015286776630734522 1107 66-67 -0.3430144181908332 1107 68-69 -0.32105956732530316 1107 70-71 -0.5907500652760298 1107 72-73 -0.4926326742629925 1107 74-75 -0.5980619280172981 1107 76-77 -0.41946281789803663 1107 78-79 -0.30159446734687023 1107 80-81 -0.36342570049060186 1107 82-83 -0.133732395439651 1107 84-85 0.18440219263538893 1107 86-87 0.16296278363328298 1107 88-89 0.1044540599409629 1107 90-91 0.13495346877019898 1107 92-93 0.7208180033123099 1107 94-95 0.9813334394105198 1107 96-97 0.8388270406222738 1107 98-99 0.933915304382829 1107 100-101 1.57850902442868 1108 1 0.12197147641601092 1108 2 -0.06259742710221161 1108 3 -0.07592425591922591 1108 4 0.029152381844596675 1108 5 0.12586097386655837 1108 6 -0.01325801975293217 1108 7 -0.13910675265089623 1108 8 0.19807976031329844 1108 9 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2316 70-71 -0.706229157542154 2316 72-73 -0.579782873609215 2316 74-75 -0.733603186071452 2316 76-77 -0.5095606959472789 2316 78-79 -0.3314586095912695 2316 80-81 -0.14940722081258073 2316 82-83 0.19151371683667406 2316 84-85 -0.3602428577207917 2316 86-87 -0.6885006632612658 2316 88-89 -0.8653227856649188 2316 90-91 -1.1741796113650942 2316 92-93 -0.9644842282316297 2316 94-95 -0.9581541179841899 2316 96-97 -1.301088497045658 2316 98-99 -1.2267756995285097 2316 100-101 -1.5251084953642255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 15.0 5 61.0 6 147.0 7 257.0 8 443.0 9 739.0 10 1184.0 11 1731.0 12 2284.0 13 2939.0 14 3410.0 15 4411.0 16 5627.0 17 7269.0 18 6898.0 19 4429.0 20 3860.0 21 3959.0 22 4067.0 23 4382.0 24 4783.0 25 5061.0 26 5351.0 27 5769.0 28 6753.0 29 7636.0 30 8906.0 31 10283.0 32 12143.0 33 14692.0 34 19627.0 35 28604.0 36 33935.0 37 46132.0 38 32878.0 39 2565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22263353946938 21.97051075686413 16.79287128451071 19.01398441915578 2 16.341727235552504 24.094764473875134 38.49048266037014 21.07302563020222 3 18.12647574826208 29.4044902320305 33.103589283594296 19.365444736113126 4 12.548971757970689 21.22340355366777 35.35859858328167 30.86902610507987 5 11.549625622587987 39.75360358874559 33.509252234719796 15.18751855394663 6 26.382668575084594 37.780408814780124 18.416782644829794 17.420139965305488 7 22.99792900579087 38.367805933332455 21.627709111055417 17.006555949821262 8 25.140154862879076 34.078077800788826 21.271880647416534 19.509886688915564 9 26.01010434117321 17.525293830547824 22.009589890382408 34.45501193789656 10-11 23.152429131105805 29.179418011054096 26.511693862206336 21.156458995633763 12-13 25.377593689403632 28.159090609294413 27.789081771293645 18.67423393000831 14-15 22.520248255484177 28.484249891173867 28.59653866955111 20.39896318379084 16-17 18.57001991900484 31.90322133839883 29.39834052264303 20.128418219953303 18-19 18.087891938951838 27.91109894603543 35.006562545344224 18.994446569668508 20-21 19.69373689139812 26.963816961046845 35.36832697964621 17.97411916790882 22-23 19.351923914047145 27.870371591763508 35.18282789642391 17.594876597765438 24-25 18.656951445328794 28.634675029803567 35.47391383924376 17.23445968562388 26-27 18.33101387716336 28.751747836639936 35.589086903756865 17.32815138243985 28-29 17.856883747312324 28.39273701011753 36.11329129786701 17.637087944703136 30-31 18.181548364969856 28.891358545819095 35.320509438193355 17.60658365101769 32-33 17.899589758471947 28.947090714822778 35.22470946721366 17.928610059491618 34-35 17.765260321679566 29.229176079493175 35.48072198165694 17.524841617170313 36-37 18.329941587205255 29.006850557576673 34.59712982416792 18.066078031050147 38-39 17.842707524253836 28.700410126006144 34.986700175956756 18.470182173783268 40-41 17.779646623298458 28.879599627330215 34.996495914648726 18.344257834722598 42-43 18.043646029840506 29.038923261612336 34.40909330765273 18.508337400894423 44-45 17.750291861515834 29.448826947557265 33.979441076952504 18.821440113974393 46-47 18.3697462364173 29.306643362408693 32.81745181130147 19.50615858987254 48-49 18.47956745633258 29.514938355272164 32.14545418569104 19.86004000270422 50-51 18.114933583083804 30.004188157078975 32.006588927436056 19.874289332401165 52-53 18.22293527153768 30.23882388634595 31.927772428075823 19.61046841404055 54-55 17.844846917912122 30.270812172697177 31.453884103470564 20.43045680592014 56-57 18.393147172549114 30.584452524053322 30.520804992868513 20.50159531052905 58-59 17.891734133592756 30.52797335399937 31.331475588240142 20.248816924167727 60-61 18.13486881158732 30.84692046908597 30.823175960003695 20.19503475932302 62-63 17.93490537882273 31.0252262680326 30.88309251390421 20.15677583924046 64-65 17.81060220064341 30.92030953452748 30.86441127924559 20.404676985583528 66-67 17.79385720457626 30.843257469073304 30.50274226049331 20.860143065857127 68-69 17.988358863587646 30.55699375072139 30.40249311590021 21.05215426979076 70-71 17.916243453943466 30.33215053621602 30.377824532707198 21.37378147713332 72-73 17.842373596802492 30.077893126145973 30.188203247635503 21.89153002941603 74-75 17.773373412326105 30.297838801323728 29.86401613272896 22.064771653621207 76-77 17.642681906747992 29.802135217225604 30.44755090155266 22.107631974473744 78-79 17.495121073330846 29.76954294941436 30.472570690776763 22.262765286478025 80-81 17.459424966486335 29.606624576029443 30.230234356578816 22.703716100905407 82-83 17.51345076681325 29.318467000070903 30.016769144773615 23.151313088342228 84-85 17.59091928399003 28.946925826748803 29.958019496365868 23.504135392895304 86-87 17.581184237672787 28.886486486486483 29.838828141118217 23.69350113472251 88-89 17.695486868272408 28.4126443133349 29.857514882183406 24.03435393620929 90-91 17.493859364551817 28.274895228511088 29.945114967260977 24.286130439676118 92-93 17.640278991541216 28.110706217949776 29.801638992860326 24.44737579764869 94-95 17.535904496512604 27.7654294524049 29.930581891932 24.768084159150494 96-97 17.700404965109684 27.384116661610097 29.87326702634252 25.042211346937698 98-99 17.470221213840045 27.41049875344616 29.86650661530953 25.252773417404267 100-101 17.73451811887539 26.884067888277514 30.544283530678502 24.837130462168595 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4704.0 1 3002.5 2 1305.0 3 1250.5 4 1077.0 5 1065.0 6 1577.0 7 2504.5 8 3239.5 9 3460.5 10 2812.5 11 1992.0 12 1803.5 13 1780.5 14 1763.5 15 1676.0 16 1603.5 17 1581.5 18 1600.0 19 1657.0 20 1731.0 21 1850.5 22 2010.5 23 2215.5 24 2357.0 25 2573.0 26 2909.0 27 3206.0 28 3443.5 29 3735.0 30 4105.0 31 4489.5 32 5109.5 33 5602.5 34 5853.0 35 6326.5 36 6943.5 37 7374.0 38 7556.5 39 7664.5 40 7751.5 41 7985.0 42 8043.5 43 8294.5 44 8855.5 45 8982.0 46 8934.5 47 9061.5 48 9331.0 49 9335.5 50 8939.0 51 8431.5 52 7871.5 53 7502.5 54 7298.5 55 6988.5 56 6538.5 57 6004.5 58 5574.0 59 5008.0 60 4323.0 61 3689.0 62 3049.0 63 2284.0 64 1603.0 65 1234.0 66 969.0 67 775.5 68 561.5 69 372.0 70 285.5 71 228.0 72 199.5 73 192.0 74 168.5 75 131.5 76 102.0 77 68.5 78 37.0 79 20.0 80 15.5 81 11.5 82 5.5 83 3.0 84 4.0 85 5.0 86 2.5 87 1.5 88 1.0 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22589666134627812 2 0.0 3 0.0 4 0.0 5 0.021765225764750887 6 0.005935970663113878 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002638209183606168 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.648880739753855E-4 26-27 0.001319104591803084 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.004946642219261565 36-37 0.016159031249587782 38-39 0.012036829400203142 40-41 0.0061008587370892636 42-43 0.007914627550818506 44-45 0.00329776147950771 46-47 3.29776147950771E-4 48-49 0.002143544961680012 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011542165178276985 58-59 3.29776147950771E-4 60-61 0.002638209183606168 62-63 1.648880739753855E-4 64-65 0.002143544961680012 66-67 0.0062657468110646495 68-69 3.29776147950771E-4 70-71 0.0 72-73 0.0 74-75 4.946642219261566E-4 76-77 0.05787571396536031 78-79 0.13289978762416071 80-81 4.946642219261566E-4 82-83 1.648880739753855E-4 84-85 0.0 86-87 0.09843818016330515 88-89 0.3393396562413434 90-91 0.37924257014338664 92-93 3.29776147950771E-4 94-95 3.29776147950771E-4 96-97 0.0 98-99 0.0 100-101 8.244403698769275E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 303236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.89673952474948 #Duplication Level Percentage of deduplicated Percentage of total 1 83.2679155184147 49.874766465753304 2 9.793268013913732 11.731696466509028 3 2.701131119048375 4.853668411795068 4 1.2500518124182096 2.99496111203418 5 0.7241935701026988 2.1688416816969878 6 0.4799177041348269 1.7247303430727705 7 0.3249127514758365 1.3622850110392488 8 0.25167004737178067 1.2059372218887126 9 0.18546401409993016 0.9997820769382184 >10 0.9647412094346142 10.096943850658526 >50 0.03084580016286608 1.240836412167751 >100 0.019278625101791298 2.0250608609108367 >500 0.00220327144020472 0.9547940911495538 >1k 0.00330490716030708 5.280400631944923 >5k 0.00110163572010236 3.485295362440873 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5530 1.8236620981677638 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5034 1.6600931287841811 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4920 1.6224986479177934 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3794 1.2511707053252252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3170 1.0453903890039442 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1841 0.6071178883773695 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.4138690656782176 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 1025 0.33802055164954026 TruSeq Adapter, Index 1 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 966 0.3185637589204448 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.26909733672782915 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.19192971810734874 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 530 0.17478135841390866 RNA PCR Primer, Index 1 (95% over 23bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 492 0.16224986479177933 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 354 0.11674075637457294 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 348 0.11476209948686832 TruSeq Adapter, Index 1 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2977614795077104E-4 0.0 12-13 0.0 0.0 0.0 4.946642219261566E-4 0.0 14-15 0.0 0.0 0.0 6.595522959015421E-4 0.0 16-17 0.0 0.0 0.0 0.0011542165178276987 0.0 18-19 0.0 0.0 0.0 0.001648880739753855 0.0 20-21 0.0 0.0 0.0 0.0028030972575815534 0.0 22-23 0.0 0.0 0.0 0.007255075254916962 0.0 24-25 0.0 0.0 0.0 0.016159031249587782 0.0 26-27 0.0 0.0 0.0 0.030669181759421703 0.0 28-29 0.0 0.0 0.0 0.05754593781740954 0.0 30-31 0.0 0.0 0.0 0.12267672703768683 0.0 32-33 0.0 0.0 0.0 0.23463572926697357 0.0 34-35 0.0 0.0 0.0 0.418321043675553 0.0 36-37 0.0 0.0 0.0 0.6905512538089145 0.0 38-39 0.0 0.0 0.0 0.9916368768879684 0.0 40-41 0.0 0.0 0.0 1.2900842907834162 0.0 42-43 0.0 0.0 0.0 1.5870477120130855 0.0 44-45 0.0 0.0 0.0 1.9697529317099554 0.0 46-47 0.0 0.0 0.0 2.4174240525531268 0.0 48-49 0.0 0.0 0.0 2.862951628434619 0.0 50-51 0.0 0.0 0.0 3.2865490904773837 0.0 52-53 0.0 0.0 0.0 3.7004181561556013 0.0 54-55 0.0 0.0 0.0 4.167051405505942 0.0 56-57 0.0 0.0 0.0 4.752898732340487 0.0 58-59 0.0 0.0 0.0 5.31632128111438 0.0 60-61 0.0 0.0 0.0 5.910577899721669 0.0 62-63 0.0 0.0 0.0 6.474000448495561 0.0 64-65 0.0 0.0 0.0 7.079139679985226 0.0 66-67 0.0 0.0 0.0 7.7739780237175005 0.0 68-69 0.0 0.0 0.0 8.510038385943622 0.0 70-71 0.0 0.0 0.0 9.296884274954161 0.0 72-73 0.0 0.0 0.0 10.088347030036012 0.0 74-75 0.0 0.0 0.0 10.884096875041223 0.0 76-77 0.0 0.0 0.0 11.730797134904826 0.0 78-79 0.0 0.0 0.0 12.652191692279281 0.0 80-81 0.0 0.0 0.0 13.57375113772771 0.0 82-83 0.0 0.0 0.0 14.490034164808929 0.0 84-85 0.0 0.0 0.0 15.433523724096084 0.0 86-87 0.0 0.0 0.0 16.411145114696144 0.0 88-89 0.0 0.0 0.0 17.432461844899684 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2400 0.0 47.541477 1 GTATCAA 3785 0.0 33.913338 1 TATCAAC 4785 0.0 26.876415 2 ATCAACG 4855 0.0 26.391163 3 TCAACGC 4880 0.0 26.255962 4 CAACGCA 4965 0.0 25.806465 5 AACGCAG 5030 0.0 25.46878 6 ATATAGG 100 0.0016599357 23.72761 2 GAGTACT 3745 0.0 23.505857 12-13 GTATTAT 145 2.3113525E-5 22.951057 1 GAAGTGT 105 0.002206685 22.593998 6 GTACATG 2690 0.0 22.268497 1 ACGCAGA 5725 0.0 22.211182 7 AAGTGTG 150 3.0497E-5 22.142118 7 CGCAGAG 5765 0.0 21.97477 8 GTACTTT 4040 0.0 21.613268 14-15 TACATGG 2735 0.0 21.515347 2 AGTACTT 3745 0.0 21.35168 12-13 ACATGGG 2650 0.0 21.31008 3 CATGGGG 650 0.0 21.172329 4 >>END_MODULE