FastQCFastQC Report
Thu 26 May 2016
SRR938853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840230
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67190.7996619973102603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51690.6151886983325994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.41917094128988486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23550.28028039941444605No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.1436511431393785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10860.12925032431596112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.1096128441022101No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.10104376182711876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35050.046.114581
GTATCAA57350.037.1921231
TAGCGGT600.00400326631.5807024
TATCAAC70600.029.7243292
ATCAACG71900.029.3186633
TCAACGC71900.029.2527794
TAGGACG1652.7230271E-928.7097284
CAACGCA73900.028.4610925
AACGCAG74550.028.2596446
ACGCAGA82700.025.1312057
CGCAGAG83350.024.7080238
GCAGAGT83750.023.9681999
AGGACGT2202.235538E-923.6855265
GAGTACT51800.022.39188412-13
GTACATG39600.022.1436121
GGACGTG2205.8356818E-821.5194726
TACATGG40300.021.5109252
CGGGAGT2257.354174E-821.05384
CAGAGTA83100.021.0507610-11
AGTACTT52300.021.04629312-13
Produced by FastQC (version 0.11.5)