##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938853_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 840230 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.592392559180226 33.0 31.0 34.0 30.0 34.0 2 31.63277792985254 33.0 31.0 34.0 30.0 34.0 3 31.62659152851005 33.0 31.0 34.0 28.0 34.0 4 35.20605667495805 37.0 35.0 37.0 33.0 37.0 5 35.24054128036371 37.0 35.0 37.0 33.0 37.0 6 35.33527367506516 37.0 35.0 37.0 33.0 37.0 7 35.250246956190566 37.0 35.0 37.0 33.0 37.0 8 35.236217464265735 37.0 35.0 37.0 32.0 37.0 9 36.79914785237376 39.0 37.0 39.0 33.0 39.0 10-11 36.708191804624924 39.0 37.0 39.0 32.0 39.0 12-13 36.589602846839554 39.0 37.0 39.0 32.0 39.0 14-15 37.722036823250775 40.0 37.5 41.0 32.0 41.0 16-17 37.66037096985349 40.0 37.0 41.0 32.0 41.0 18-19 37.64124346905014 40.0 37.0 41.0 32.0 41.0 20-21 37.55751817954608 40.0 37.0 41.0 32.0 41.0 22-23 37.44720552705807 40.0 37.0 41.0 32.0 41.0 24-25 37.40328898039823 40.0 37.0 41.0 32.0 41.0 26-27 37.290274091617775 40.0 37.0 41.0 31.0 41.0 28-29 37.14608381038525 40.0 36.5 41.0 31.0 41.0 30-31 36.87392380657677 39.0 36.0 41.0 30.5 41.0 32-33 36.58189543339324 39.0 35.0 41.0 30.0 41.0 34-35 36.20884698237387 39.0 35.0 41.0 30.0 41.0 36-37 35.90506646989515 39.0 35.0 41.0 29.0 41.0 38-39 35.69250383823477 39.0 35.0 41.0 27.5 41.0 40-41 35.45290991752258 39.0 35.0 40.5 27.0 41.0 42-43 35.11982195351273 38.0 35.0 40.0 25.5 41.0 44-45 34.93289515965866 39.0 35.0 41.0 24.0 41.0 46-47 34.58458457803221 39.0 34.5 41.0 22.5 41.0 48-49 34.23774561727146 39.0 34.0 41.0 19.0 41.0 50-51 33.04170286707211 38.0 32.5 40.0 14.5 40.5 52-53 33.101160396558086 37.5 33.0 39.5 14.0 40.5 54-55 33.58347714316318 38.0 33.5 40.0 12.5 41.0 56-57 33.48823417397617 38.0 33.5 40.0 9.0 41.0 58-59 33.26077681111124 38.0 33.0 40.0 7.5 41.0 60-61 32.97277055092058 37.5 33.0 40.0 6.0 41.0 62-63 32.61606405389 37.0 32.5 40.0 2.0 41.0 64-65 32.21354450567107 36.0 32.0 39.5 2.0 41.0 66-67 31.822531925782226 36.0 31.5 39.0 2.0 41.0 68-69 31.406904657058185 35.0 31.0 39.0 2.0 40.5 70-71 30.977153874534352 35.0 31.0 38.5 2.0 40.0 72-73 30.546167715982527 35.0 30.5 37.5 2.0 40.0 74-75 30.0821197767278 35.0 30.0 37.0 2.0 39.0 76-77 29.617566618663936 35.0 29.5 36.5 2.0 39.0 78-79 29.171808909465263 34.5 29.0 36.0 2.0 38.5 80-81 28.723311474239196 34.0 29.0 35.5 2.0 37.0 82-83 28.239357675874462 34.0 28.0 35.0 2.0 37.0 84-85 27.805045642264616 34.0 27.0 35.0 2.0 36.5 86-87 27.386110945812455 34.0 26.0 35.0 2.0 36.0 88-89 26.992099187127337 34.0 25.5 35.0 2.0 36.0 90-91 26.55876545707723 34.0 25.0 35.0 2.0 35.0 92-93 26.086585815788535 33.5 23.5 35.0 2.0 35.0 94-95 25.5552229746617 33.0 19.5 35.0 2.0 35.0 96-97 24.964011044594933 33.0 12.0 35.0 2.0 35.0 98-99 24.286181759756257 32.5 2.0 35.0 2.0 35.0 100-101 22.297457838925055 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16224021190419435 1101 2 -0.02960105931870416 1101 3 0.23670053237627187 1101 4 0.08466427195958914 1101 5 -0.15577110561662266 1101 6 -0.05965485023243389 1101 7 0.03568964640835759 1101 8 0.12989980322038264 1101 9 0.17900035914997403 1101 10-11 0.18130437488573392 1101 12-13 -0.05627473719827236 1101 14-15 0.23079834302004087 1101 16-17 0.46409042091229935 1101 18-19 0.4463969900314311 1101 20-21 0.4797929805131602 1101 22-23 0.39757539066813763 1101 24-25 0.3602946416221684 1101 26-27 0.2518883016878064 1101 28-29 0.33889811050059393 1101 30-31 0.25806267331578425 1101 32-33 0.3799547436069233 1101 34-35 0.21068722725258482 1101 36-37 0.49648431727784725 1101 38-39 0.5076474522035639 1101 40-41 0.4715751652778124 1101 42-43 0.298795958961243 1101 44-45 0.14512982602497004 1101 46-47 0.285256533321423 1101 48-49 0.43800557948014784 1101 50-51 0.3835701207382982 1101 52-53 0.4450727199502822 1101 54-55 0.5342979610777547 1101 56-57 0.5793494175066272 1101 58-59 0.5676213240138352 1101 60-61 0.5924725173726486 1101 62-63 0.6433239612802666 1101 64-65 0.44198641750475787 1101 66-67 0.4885670310061734 1101 68-69 0.4398109024985537 1101 70-71 0.16289328236151945 1101 72-73 0.055580111521582154 1101 74-75 0.14599278406354088 1101 76-77 0.3397921464362206 1101 78-79 0.15362863382038938 1101 80-81 -0.025116782432878182 1101 82-83 -0.007751284208165288 1101 84-85 -0.19923769823919102 1101 86-87 -0.44781709654239776 1101 88-89 -0.4366617054598443 1101 90-91 -0.5274214061309763 1101 92-93 -0.6318492338326251 1101 94-95 -0.8045410644462052 1101 96-97 -0.5872936949141518 1101 98-99 -0.6922093633025206 1101 100-101 -0.7077872212839047 1102 1 0.05146820251774287 1102 2 0.11530889928598498 1102 3 0.17759685953107152 1102 4 0.18076665284919358 1102 5 0.08002067860042672 1102 6 0.055648891165517966 1102 7 0.2058520636836434 1102 8 0.09239683699579615 1102 9 0.26466822109529176 1102 10-11 0.30216649158360553 1102 12-13 0.3614290094729711 1102 14-15 0.14157101156750684 1102 16-17 0.10603250702509825 1102 18-19 0.3066317831867025 1102 20-21 0.3155663082216549 1102 22-23 0.22915645429036147 1102 24-25 0.12334602981527354 1102 26-27 0.08891073952892015 1102 28-29 0.052097150762151045 1102 30-31 0.0398869673543345 1102 32-33 -0.1437173765185804 1102 34-35 0.051166964642838764 1102 36-37 -0.11616300521009038 1102 38-39 0.11264521105609049 1102 40-41 0.01847776764551412 1102 42-43 -0.15802899423832883 1102 44-45 -0.024582713854272242 1102 46-47 -0.1769224552619093 1102 48-49 -0.26431895185636733 1102 50-51 -0.41139619613359457 1102 52-53 -0.37260663776321934 1102 54-55 -0.4846183122894985 1102 56-57 -0.5573836486467769 1102 58-59 -0.6260418315218033 1102 60-61 -0.6368875031931722 1102 62-63 -0.506966399148979 1102 64-65 -0.5334812572388259 1102 66-67 -0.8109573843180051 1102 68-69 -0.8886144277411177 1102 70-71 -1.2758920816271946 1102 72-73 -1.1884660165665863 1102 74-75 -1.2964393882675154 1102 76-77 -1.4618811338126818 1102 78-79 -1.4538920638356316 1102 80-81 -1.3899805338500713 1102 82-83 -1.3180134325457615 1102 84-85 -1.6186882216130414 1102 86-87 -1.495128378742411 1102 88-89 -1.6639700873513732 1102 90-91 -1.5832754151482966 1102 92-93 -1.9222310858114255 1102 94-95 -1.9808534540367333 1102 96-97 -1.97370377216782 1102 98-99 -1.9805659430478215 1102 100-101 -1.507514328621685 1103 1 0.03222695154616417 1103 2 0.13757695448767038 1103 3 0.08564457886553001 1103 4 0.19912098729090388 1103 5 0.1257791212395034 1103 6 0.11160364874704243 1103 7 0.13597110400770873 1103 8 -0.04865514276315963 1103 9 0.03899795408503337 1103 10-11 -0.009796722343892839 1103 12-13 0.012441868150077084 1103 14-15 0.15152382760812344 1103 16-17 0.17551919198911747 1103 18-19 0.3445603654425611 1103 20-21 0.2777691508697657 1103 22-23 0.26612071639193857 1103 24-25 0.059434018385339016 1103 26-27 0.27550668938427947 1103 28-29 0.2382818938627409 1103 30-31 0.35055367057270814 1103 32-33 0.14725252314697457 1103 34-35 0.20907680877128598 1103 36-37 0.28924734687964815 1103 38-39 0.13697494404584631 1103 40-41 0.4305964338520596 1103 42-43 0.3987264070833447 1103 44-45 0.2976816324237319 1103 46-47 0.3446917005709409 1103 48-49 0.22306183849914163 1103 50-51 0.40494838545144773 1103 52-53 0.2619644010256721 1103 54-55 0.4589568643681474 1103 56-57 0.34264715154544234 1103 58-59 0.48564192307000553 1103 60-61 0.5621841370863834 1103 62-63 0.6681326936160374 1103 64-65 0.2940529858021037 1103 66-67 0.006982840733030571 1103 68-69 0.027854967188297053 1103 70-71 -0.030404874188707964 1103 72-73 0.24791949218485954 1103 74-75 0.29128633198933684 1103 76-77 -0.19151188627266436 1103 78-79 -0.399309608012139 1103 80-81 -0.4858142837654107 1103 82-83 -0.42854258307323434 1103 84-85 -0.29626906808617903 1103 86-87 -0.5254665680781265 1103 88-89 -0.4728823084161249 1103 90-91 -0.5810202282991774 1103 92-93 -0.7520334163055509 1103 94-95 -0.79495655566447 1103 96-97 -1.0338963781866006 1103 98-99 -0.6075965787573381 1103 100-101 -0.6095468187930386 1104 1 0.12859751289058252 1104 2 0.3163361418497672 1104 3 0.16101019061125044 1104 4 0.3357597146559854 1104 5 0.014343449198086944 1104 6 0.17368754356365912 1104 7 0.1976415975735506 1104 8 0.09779465382099772 1104 9 0.12561513010584235 1104 10-11 0.2365046984425021 1104 12-13 0.21227410415411896 1104 14-15 0.3825277997852581 1104 16-17 0.24955967646975097 1104 18-19 0.3384426678632053 1104 20-21 0.4330590044327849 1104 22-23 0.5360252655682558 1104 24-25 0.24048694175709073 1104 26-27 0.40903929921201154 1104 28-29 0.5510832321291161 1104 30-31 0.3399670734258251 1104 32-33 0.3888222621754096 1104 34-35 0.3605827590253341 1104 36-37 0.3805393219281612 1104 38-39 0.41101270456474026 1104 40-41 0.21087126556730595 1104 42-43 0.18123809207799724 1104 44-45 0.3454967747667581 1104 46-47 0.4428881174396295 1104 48-49 0.5380912172275671 1104 50-51 0.45805121821140915 1104 52-53 0.541921971315908 1104 54-55 0.5038621746514238 1104 56-57 0.46082382770324415 1104 58-59 0.7015920008631511 1104 60-61 0.4277213744443955 1104 62-63 0.47966498618234965 1104 64-65 0.4928180697401885 1104 66-67 0.3339696102906693 1104 68-69 0.4419043606261148 1104 70-71 0.3431279920118975 1104 72-73 0.2288573682317825 1104 74-75 0.14561126216778675 1104 76-77 0.02604933476721527 1104 78-79 -0.12769136644159573 1104 80-81 -0.251939736624049 1104 82-83 -0.4201559856058772 1104 84-85 -0.36561071858266203 1104 86-87 -0.12136043611980796 1104 88-89 -0.3705470720483497 1104 90-91 -0.4160186500037035 1104 92-93 -0.5114266778651597 1104 94-95 -0.6063494454834242 1104 96-97 -0.5161161185770204 1104 98-99 -0.5218246320380295 1104 100-101 0.012404947856378357 1105 1 0.17008700173781577 1105 2 0.27902658179592166 1105 3 0.24061754778915656 1105 4 0.21726864064387996 1105 5 0.25356338453342175 1105 6 0.20795258061374255 1105 7 0.35708905439039285 1105 8 0.2766666706287779 1105 9 0.20617704589081143 1105 10-11 0.32702500364964493 1105 12-13 0.4085805756526 1105 14-15 0.46990215030798765 1105 16-17 0.5441149182073914 1105 18-19 0.4516600807734932 1105 20-21 0.5988919393781273 1105 22-23 0.5661984385399421 1105 24-25 0.6362018070809796 1105 26-27 0.5342004890921146 1105 28-29 0.5251796252500043 1105 30-31 0.6931639798381894 1105 32-33 0.7697296747082802 1105 34-35 0.8342773568841082 1105 36-37 0.6662684348648682 1105 38-39 0.84623324377241 1105 40-41 0.8106202811296015 1105 42-43 0.6056054414558858 1105 44-45 0.9232060228199046 1105 46-47 0.8199993117229738 1105 48-49 0.5388782956177423 1105 50-51 0.6073248415711987 1105 52-53 0.6081073223796025 1105 54-55 0.6825321143899998 1105 56-57 0.6378048120151476 1105 58-59 0.5015679403249464 1105 60-61 0.3834161735939432 1105 62-63 0.5057690975464695 1105 64-65 0.6197704330197169 1105 66-67 0.4140416660985444 1105 68-69 0.5583101879941132 1105 70-71 0.8289071131260393 1105 72-73 0.6814030675285245 1105 74-75 0.6456993268827738 1105 76-77 0.4699340266036174 1105 78-79 0.3121274089556394 1105 80-81 0.39766570200113804 1105 82-83 0.2657635672769807 1105 84-85 0.39578501279479994 1105 86-87 0.1588418296595009 1105 88-89 0.08702539263140707 1105 90-91 0.22883050772020397 1105 92-93 0.46684807146491636 1105 94-95 0.5564240269082887 1105 96-97 0.5351226059942924 1105 98-99 0.6807619544478136 1105 100-101 0.5854530563521045 1106 1 0.1167460333126158 1106 2 0.059733197233587276 1106 3 0.1218302959296409 1106 4 0.22070443198420975 1106 5 0.15660387514964924 1106 6 0.15358949505145603 1106 7 0.16626101636270363 1106 8 0.1922619615011456 1106 9 0.2075624456248164 1106 10-11 0.20605497754254998 1106 12-13 0.23083994711213052 1106 14-15 0.23334360639388763 1106 16-17 0.10815425124517475 1106 18-19 0.06739637455212488 1106 20-21 0.20618037344541307 1106 22-23 0.06451132953053218 1106 24-25 0.0339623853776132 1106 26-27 -0.01688381709969633 1106 28-29 0.1587998902615766 1106 30-31 0.17572949385227332 1106 32-33 0.3711809578962999 1106 34-35 0.3340587715927441 1106 36-37 0.6456290574942969 1106 38-39 0.6202160525010498 1106 40-41 0.6840501614310597 1106 42-43 0.8401228297618815 1106 44-45 0.7386153622613136 1106 46-47 0.6573810909456697 1106 48-49 0.930315293795168 1106 50-51 0.7070397570926019 1106 52-53 0.6235745407432276 1106 54-55 0.4326829690276952 1106 56-57 0.5209109397921026 1106 58-59 0.3399164206953742 1106 60-61 0.608420483726448 1106 62-63 0.4056046967780276 1106 64-65 0.36324673960559295 1106 66-67 0.2876162437055534 1106 68-69 0.6002169906146761 1106 70-71 0.6872207429964909 1106 72-73 0.5647770392540572 1106 74-75 0.4636762738312008 1106 76-77 0.25989203359834434 1106 78-79 0.3826350194181778 1106 80-81 0.1697305017646933 1106 82-83 0.28484821827939655 1106 84-85 0.3071286658168155 1106 86-87 0.45373185970727903 1106 88-89 0.4082503322895512 1106 90-91 0.3807503638849248 1106 92-93 0.544104117965091 1106 94-95 0.7739133131503451 1106 96-97 0.5978565846160357 1106 98-99 0.53999302680878 1106 100-101 0.6425807840502493 1107 1 0.004408144949845649 1107 2 -0.06881735519126053 1107 3 -0.14339814569816767 1107 4 -0.0399719808850989 1107 5 -0.15262951159575522 1107 6 -0.0719429752868379 1107 7 -0.1525790414903696 1107 8 -0.054126722341401035 1107 9 -0.169694814515978 1107 10-11 0.02333318241685589 1107 12-13 -0.02086068723122736 1107 14-15 -0.12869421415355475 1107 16-17 -0.14669934566773435 1107 18-19 -0.15859069852639607 1107 20-21 -0.19663216218764745 1107 22-23 -0.1126359557559482 1107 24-25 0.09389493013272698 1107 26-27 0.11864181169240595 1107 28-29 -0.03258411252305393 1107 30-31 0.13220615757828114 1107 32-33 0.3273583039765171 1107 34-35 0.159726872583569 1107 36-37 0.07262405836448949 1107 38-39 0.2931796422723352 1107 40-41 0.3130358121881329 1107 42-43 0.6025621410462279 1107 44-45 0.4674245731429636 1107 46-47 0.6219130751741062 1107 48-49 0.6475868971775398 1107 50-51 0.6012673525291774 1107 52-53 0.4182253818333166 1107 54-55 0.3026928412588248 1107 56-57 0.3908615486297009 1107 58-59 0.6241023967259522 1107 60-61 0.45052329896268617 1107 62-63 0.5172816857775473 1107 64-65 0.33377707946240065 1107 66-67 0.49488208578586423 1107 68-69 0.38534442941584146 1107 70-71 0.646528546560095 1107 72-73 0.39449530558224666 1107 74-75 0.5173148961598457 1107 76-77 0.4739596628825673 1107 78-79 0.426285884513689 1107 80-81 0.5626107927104478 1107 82-83 0.5731443732104111 1107 84-85 0.6095587650192336 1107 86-87 0.6522304703932953 1107 88-89 0.9478330279787777 1107 90-91 1.115436402929749 1107 92-93 1.0481535331329184 1107 94-95 0.9998531255479506 1107 96-97 1.110906533904572 1107 98-99 1.1119267838378697 1107 100-101 1.4473214054306816 1108 1 0.08131720555445199 1108 2 0.14505308491461832 1108 3 -0.037126093085472434 1108 4 0.0659017475142889 1108 5 0.1546027566205126 1108 6 0.010810998774061886 1108 7 0.1257026839032207 1108 8 0.04294997343212259 1108 9 0.08871872511728185 1108 10-11 0.2388972066779047 1108 12-13 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2316 86-87 -0.972445818336066 2316 88-89 -1.2024642019324467 2316 90-91 -1.3742433985715259 2316 92-93 -1.447731161183622 2316 94-95 -1.4694869433096116 2316 96-97 -1.4019545495234595 2316 98-99 -1.5988594142824688 2316 100-101 -2.2447476835021014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4742.0 3 1105.0 4 958.0 5 1045.0 6 1104.0 7 1333.0 8 1611.0 9 2030.0 10 2601.0 11 3609.0 12 5143.0 13 6935.0 14 7418.0 15 7563.0 16 9497.0 17 12636.0 18 10216.0 19 6671.0 20 6477.0 21 6797.0 22 7156.0 23 7876.0 24 8907.0 25 10118.0 26 11603.0 27 13330.0 28 15683.0 29 18302.0 30 21658.0 31 25821.0 32 31153.0 33 38776.0 34 50501.0 35 71633.0 36 99926.0 37 146445.0 38 137352.0 39 24488.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82562631792884 19.550584030963954 14.620144449172798 22.003645201934415 2 15.848664179424505 21.950388572027578 39.27090927283903 22.93003797570889 3 18.29366634466148 26.337562281797812 31.78119688254514 23.587574490995568 4 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20.445489254572433 24-25 20.996302249559147 26.737552624009233 31.906958905480167 20.359186220951457 26-27 20.72586720822824 27.09721605984176 31.682066827666095 20.494849904263905 28-29 20.460826529354424 27.15795453665752 31.851910959829976 20.52930797415808 30-31 20.744003705966342 26.97894672911553 31.706137828127474 20.57091173679065 32-33 20.387663404286478 27.372141745943956 31.38356553407162 20.856629315697944 34-35 21.08355644575056 27.202725333594348 31.15685827477503 20.556859945880067 36-37 21.20296868106049 26.918528668673048 30.92579373477726 20.9527089154892 38-39 20.8260236233886 27.01088999840407 31.233835205316428 20.929251172890904 40-41 21.020965748693076 26.87283822836914 31.261159940623738 20.84503608231405 42-43 20.937836613238936 27.17437955567351 30.73074886096954 21.157034970118012 44-45 20.746503232085434 27.456495526253335 30.55054553584826 21.246455705812973 46-47 21.053651001270943 27.44160299000343 29.909061236006778 21.595684772718847 48-49 21.206992008552273 27.62735435607442 29.54521810565739 21.620435529715916 50-51 21.010713346246742 28.012710141808356 29.234641413779084 21.74193509816582 52-53 21.30320405125047 27.861434797945677 29.192619740877813 21.642741409926042 54-55 20.818238339490897 27.802109114387175 29.36816241615553 22.0114901299664 56-57 21.01229854086324 28.10224955231303 29.11375911213333 21.7716927946904 58-59 21.139202986976198 27.495072899573202 29.20112250642107 22.164601607029528 60-61 20.92762891020092 27.49574486261985 29.641086853791148 21.93553937338808 62-63 20.861006125024133 27.368104565943383 29.702631184125504 22.06825812490698 64-65 20.701195867115775 27.316345988831237 29.57909950291298 22.403358641140006 66-67 20.705264397907325 27.680350939927965 29.128229292552994 22.486155369611716 68-69 20.799432590522084 27.326973897848355 29.337785057778525 22.535808453851033 70-71 20.778997109842145 27.470433140955148 29.06686711792119 22.68370263128152 72-73 21.048952596101465 27.290682162412335 28.952811364450394 22.707553877035807 74-75 20.802991501795844 27.471328351881734 28.83493943990658 22.890740706415833 76-77 20.912598444967067 27.255042857262417 28.99001770724108 22.842340990529436 78-79 21.045258350197287 27.327733663878785 28.873699045059926 22.753308940864 80-81 21.108881680596127 27.418289578518092 28.655235007677486 22.817593733208295 82-83 21.167946478634132 27.2330955429547 28.522770610591653 23.076187367819514 84-85 21.166106971344956 26.890600585558065 28.587798442735934 23.355494000361045 86-87 21.474239604586614 26.720293929689543 28.58748395635771 23.217982509366138 88-89 21.557190533073616 26.468460518401866 28.587374641426948 23.386974307097564 90-91 21.550012914582 26.570138138195592 28.602151952015152 23.277696995207258 92-93 21.889335068090826 26.28510372669916 28.590672967918145 23.234888237291866 94-95 21.76254346983779 25.991853740439026 28.490024060884085 23.7555787288391 96-97 22.127341262912672 25.608232060981063 28.468274108639864 23.796152567466404 98-99 22.143968832044735 25.625968991089316 28.286438100864164 23.943624076001786 100-101 22.6505705731558 25.053087695133147 28.63262119299051 23.66372053872054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2143.0 1 1368.0 2 763.5 3 902.0 4 745.0 5 712.5 6 1086.5 7 1859.0 8 2728.0 9 2976.0 10 2457.5 11 2034.5 12 2060.5 13 2212.5 14 2309.0 15 2393.0 16 2487.5 17 2544.0 18 2661.0 19 2819.5 20 3057.0 21 3357.5 22 3733.0 23 4197.5 24 4795.5 25 5600.5 26 6702.5 27 7878.0 28 8831.5 29 9781.5 30 10928.0 31 12386.5 32 14070.5 33 15485.5 34 16885.5 35 18276.0 36 19824.0 37 21456.5 38 21895.0 39 21589.5 40 21932.5 41 23592.0 42 25463.0 43 26814.5 44 27594.0 45 28371.5 46 28738.5 47 29116.0 48 31307.5 49 32135.5 50 31111.5 51 29797.0 52 28224.0 53 27539.0 54 26620.5 55 24028.0 56 21136.0 57 18577.5 58 16356.0 59 14290.5 60 12045.0 61 10033.5 62 8328.5 63 6496.5 64 4767.0 65 3681.0 66 2834.5 67 2327.5 68 1756.0 69 1175.0 70 930.5 71 812.5 72 729.5 73 619.0 74 496.0 75 417.5 76 332.0 77 211.5 78 138.0 79 109.5 80 71.5 81 56.5 82 42.5 83 29.0 84 26.5 85 21.0 86 16.0 87 14.5 88 10.0 89 6.5 90 5.0 91 3.0 92 3.0 93 4.0 94 3.5 95 2.5 96 1.0 97 2.0 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15698082667840949 2 0.08878521357247421 3 0.08319150708734513 4 0.09699725075277008 5 0.11770586625090748 6 0.038441855206312554 7 0.04141723099627483 8 0.044154576723040115 9 0.045106696975828045 10-11 0.04403556169144163 12-13 0.04724896754460088 14-15 0.04611832474441522 16-17 0.043440486533449174 18-19 0.048260595313188055 20-21 0.04980779072396844 22-23 0.06236387655760922 24-25 0.0792045035287957 26-27 0.08122775906597003 28-29 0.06980231603251491 30-31 0.059864560894040915 32-33 0.04802256524999107 34-35 0.29289599276388606 36-37 0.464039608202516 38-39 0.4437475453149733 40-41 0.4203610916058699 42-43 0.4490437142211061 44-45 0.4582673791699892 46-47 0.45737476643300046 48-49 0.4714185401616224 50-51 0.46296847291812954 52-53 0.4695142996560465 54-55 0.4593980219701748 56-57 0.4413077371672043 58-59 0.4202420765742713 60-61 0.3928091117908192 62-63 0.4455327707889507 64-65 0.4830225057424753 66-67 0.48504576127964966 68-69 0.493852873617938 70-71 0.4899253775751878 72-73 0.4812372802684979 74-75 0.47540554372017185 76-77 0.4580888566225914 78-79 0.43238160979731743 80-81 0.4395820192090261 82-83 0.4497578044106971 84-85 0.44785356390512115 86-87 0.4569582138224058 88-89 0.4676100591504707 90-91 0.47201361531961483 92-93 0.4753460362043726 94-95 0.4782023969627364 96-97 0.48034466753150923 98-99 0.47445342346738395 100-101 0.4615402925389477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 840230.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.27117121714538 #Duplication Level Percentage of deduplicated Percentage of total 1 83.76043389525418 52.15860320313761 2 9.980152846777964 12.42951613389983 3 2.6074272992167034 4.871026553573469 4 1.0830239049048718 2.697646680583706 5 0.5705023739517897 1.77629255040699 6 0.35241746021062387 1.3167268802812369 7 0.2566194223194169 1.1185994389428144 8 0.1842142582353151 0.9176990092168592 9 0.132657080953935 0.7434640621124282 >10 0.9433565821231468 11.402029617812643 >50 0.08848849170416159 3.7452885814267916 >100 0.038986396285778595 4.149392718894076 >500 5.73329354031245E-4 0.286686179660621 >1k 7.644391387083267E-4 0.9722814810407944 >5k 3.8221956935416336E-4 1.4147469090101548 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6719 0.7996619973102603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5169 0.6151886983325994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3522 0.41917094128988486 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2355 0.28028039941444605 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1207 0.1436511431393785 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1086 0.12925032431596112 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 921 0.1096128441022101 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.10104376182711876 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.165526105947181E-4 0.0 22-23 0.0 0.0 0.0 0.0019042405055758544 0.0 24-25 0.0 0.0 0.0 0.004522571200742654 0.0 26-27 0.0 0.0 0.0 0.009045142401485308 0.0 28-29 0.0 0.0 0.0 0.026183306951667994 0.0 30-31 0.0 0.0 0.0 0.06617235756876093 0.0 32-33 0.0 0.0 0.0 0.1451983385501589 0.0 34-35 0.0 0.0 0.0 0.26391583256965356 0.0 36-37 0.0 0.0 0.0 0.4481511014841174 0.0 38-39 0.0 0.0 0.0 0.7068897801792366 0.0 40-41 0.0 0.0 0.0 0.9949656641633837 0.0 42-43 0.0 0.0 0.0 1.2632850529021815 0.0 44-45 0.0 0.0 0.0 1.5694512216892993 0.0 46-47 0.0 0.0 0.0 1.9242350308844007 5.950751579924544E-5 48-49 0.0 0.0 0.0 2.2994299179986433 1.1901503159849088E-4 50-51 0.0 0.0 0.0 2.707472953834069 1.1901503159849088E-4 52-53 0.0 0.0 0.0 3.0970091522559295 1.1901503159849088E-4 54-55 0.0 0.0 0.0 3.5245111457577094 1.1901503159849088E-4 56-57 0.0 0.0 0.0 4.0138414481749045 1.1901503159849088E-4 58-59 0.0 0.0 0.0 4.538043154850458 1.1901503159849088E-4 60-61 0.0 0.0 0.0 5.111398069576188 1.1901503159849088E-4 62-63 0.0 0.0 0.0 5.6660081168251555 1.1901503159849088E-4 64-65 0.0 0.0 0.0 6.2446592004570185 1.1901503159849088E-4 66-67 0.0 0.0 0.0 6.8791283339085725 1.1901503159849088E-4 68-69 0.0 0.0 0.0 7.588457922235579 1.1901503159849088E-4 70-71 0.0 0.0 0.0 8.36824440926889 1.1901503159849088E-4 72-73 0.0 0.0 0.0 9.161301072325436 1.1901503159849088E-4 74-75 0.0 0.0 0.0 9.973578662985135 1.1901503159849088E-4 76-77 0.0 0.0 0.0 10.823524511145758 1.1901503159849088E-4 78-79 0.0 0.0 0.0 11.744284303107483 1.1901503159849088E-4 80-81 0.0 0.0 0.0 12.720148054699308 1.1901503159849088E-4 82-83 0.0 0.0 0.0 13.711602775430537 1.1901503159849088E-4 84-85 0.0 0.0 0.0 14.716565702248193 1.1901503159849088E-4 86-87 0.0 0.0 0.0 15.730573771467338 1.1901503159849088E-4 88-89 0.0 0.0 0.0 16.76808730942718 1.1901503159849088E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3505 0.0 46.11458 1 GTATCAA 5735 0.0 37.192123 1 TAGCGGT 60 0.004003266 31.580702 4 TATCAAC 7060 0.0 29.724329 2 ATCAACG 7190 0.0 29.318663 3 TCAACGC 7190 0.0 29.252779 4 TAGGACG 165 2.7230271E-9 28.709728 4 CAACGCA 7390 0.0 28.461092 5 AACGCAG 7455 0.0 28.259644 6 ACGCAGA 8270 0.0 25.131205 7 CGCAGAG 8335 0.0 24.708023 8 GCAGAGT 8375 0.0 23.968199 9 AGGACGT 220 2.235538E-9 23.685526 5 GAGTACT 5180 0.0 22.391884 12-13 GTACATG 3960 0.0 22.143612 1 GGACGTG 220 5.8356818E-8 21.519472 6 TACATGG 4030 0.0 21.510925 2 CGGGAGT 225 7.354174E-8 21.0538 4 CAGAGTA 8310 0.0 21.05076 10-11 AGTACTT 5230 0.0 21.046293 12-13 >>END_MODULE