FastQCFastQC Report
Thu 26 May 2016
SRR938853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840230
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60150.7158754150649227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53470.6363733739571308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.4330956999869084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23430.27885221903526414No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT20940.24921747616723994No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.14293705294978756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.12853623412637016No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10470.12460873808361997RNA PCR Primer, Index 27 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.11603965580852862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.046.318431
GTATCAA58150.034.8963241
TATCAAC73350.027.5578982
ATCAACG74450.027.023263
TCAACGC75050.026.7439944
CAACGCA76650.026.1857385
AACGCAG78050.025.7160386
ACGCAGA88550.022.5059477
CGCAGAG88250.022.4211548
GAGTACT56200.021.61489712-13
GCAGAGT91550.021.4574729
GTACATG42600.021.357161
TACATGG42750.020.8669192
GTAATAC1605.027822E-520.7593923
TAAGGCG3252.1827873E-1120.440025
ACATGGG43150.020.2336223
GTACTTT61900.019.51010314-15
CAGAGTA90700.019.48747610-11
AGTACTT56500.019.35851312-13
AGAGTAC84150.018.55147210-11
Produced by FastQC (version 0.11.5)