##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938853_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 840230 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.931324756316723 31.0 31.0 34.0 30.0 34.0 2 32.14698951477572 33.0 31.0 34.0 30.0 34.0 3 32.22029920378944 34.0 31.0 34.0 30.0 34.0 4 35.88913868821632 37.0 35.0 37.0 35.0 37.0 5 35.79908239410638 37.0 35.0 37.0 35.0 37.0 6 35.83102007783583 37.0 35.0 37.0 35.0 37.0 7 35.757033193292315 37.0 35.0 37.0 33.0 37.0 8 35.733395617866535 37.0 35.0 37.0 33.0 37.0 9 37.362510265046474 39.0 37.0 39.0 34.0 39.0 10-11 37.29778096473585 39.0 37.0 39.0 33.5 39.0 12-13 37.22787689085132 39.0 37.0 39.0 33.0 39.0 14-15 38.406863596872284 40.0 38.0 41.0 33.0 41.0 16-17 38.34436582840412 40.0 38.0 41.0 33.0 41.0 18-19 38.38884531616343 40.0 38.0 41.0 33.5 41.0 20-21 38.44080013805744 40.0 38.0 41.0 34.0 41.0 22-23 38.37567511276674 40.0 38.0 41.0 34.0 41.0 24-25 38.28187282053723 40.0 38.0 41.0 33.0 41.0 26-27 38.09131071254299 40.0 37.0 41.0 33.0 41.0 28-29 37.8932470871071 40.0 37.0 41.0 33.0 41.0 30-31 37.586585220713374 40.0 36.5 41.0 32.5 41.0 32-33 37.27679325898861 39.5 36.0 41.0 32.0 41.0 34-35 37.15130380967116 40.0 36.0 41.0 31.0 41.0 36-37 36.99545362579293 40.0 35.0 41.0 31.0 41.0 38-39 36.841829618080766 40.0 35.0 41.0 31.0 41.0 40-41 36.61799685800317 39.0 35.0 41.0 30.0 41.0 42-43 36.359729478833174 39.0 35.0 41.0 30.0 41.0 44-45 36.00592754364877 39.0 35.0 41.0 29.5 41.0 46-47 35.557509848493865 39.0 35.0 41.0 27.5 41.0 48-49 35.144663961058285 39.0 35.0 41.0 25.5 41.0 50-51 34.83965521345346 39.0 35.0 40.5 24.0 41.0 52-53 34.60148947312045 38.5 34.0 40.0 23.5 41.0 54-55 34.325729859681275 38.0 34.0 40.0 23.0 41.0 56-57 34.0434143032265 38.0 34.0 40.0 22.0 41.0 58-59 33.71444366423479 38.0 33.5 40.0 20.5 41.0 60-61 33.343447627435346 37.0 33.0 40.0 18.5 41.0 62-63 32.942538352593935 36.5 33.0 40.0 15.5 41.0 64-65 32.554363091058406 36.0 32.0 39.0 12.5 41.0 66-67 32.14497578044107 35.5 32.0 39.0 7.5 40.5 68-69 31.679709127262775 35.0 31.0 38.5 4.5 40.0 70-71 31.205464575175846 35.0 31.0 38.0 2.0 40.0 72-73 30.879234852361854 35.0 31.0 37.0 2.0 39.5 74-75 30.46770229579996 35.0 30.5 37.0 2.0 39.0 76-77 28.4382210823227 32.5 27.5 35.0 2.0 37.0 78-79 29.45976934886876 34.0 29.0 36.0 2.0 38.0 80-81 29.500956285778894 34.0 30.0 35.0 2.0 37.0 82-83 29.19466991180986 34.0 29.5 35.0 2.0 37.0 84-85 28.81626756959404 34.0 29.0 35.0 2.0 36.0 86-87 28.2976696856813 34.0 29.0 35.0 2.0 36.0 88-89 27.80214643609488 34.0 27.5 35.0 2.0 35.5 90-91 27.413737905097413 33.5 26.5 35.0 2.0 35.0 92-93 27.130325625126453 33.0 26.0 35.0 2.0 35.0 94-95 26.764119348273688 33.0 25.5 35.0 2.0 35.0 96-97 26.289742094426526 33.0 25.0 35.0 2.0 35.0 98-99 25.60367220879997 33.0 22.0 35.0 2.0 35.0 100-101 22.807601490068194 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03304994923666982 1101 2 -0.0017527362073153085 1101 3 0.06403629594613136 1101 4 0.1084262069376365 1101 5 -0.10683991297653961 1101 6 0.04000448428060821 1101 7 -0.02585417733096307 1101 8 0.0537192751495823 1101 9 0.05450857211967275 1101 10-11 -0.02749166403521741 1101 12-13 -0.14915299622189337 1101 14-15 -0.0786735506956262 1101 16-17 -0.02761251210050375 1101 18-19 0.051789284885657594 1101 20-21 0.11660434514762841 1101 22-23 0.18027718284796634 1101 24-25 0.06201988614648002 1101 26-27 0.22134901069383517 1101 28-29 0.22638839502752006 1101 30-31 0.10263095216499352 1101 32-33 0.09726148067277762 1101 34-35 0.044116805470331144 1101 36-37 0.052283723260238446 1101 38-39 0.0032090815286736074 1101 40-41 0.17532447512641625 1101 42-43 0.14313369707937795 1101 44-45 0.17535317665986128 1101 46-47 0.055029651280477765 1101 48-49 -0.05982269690098718 1101 50-51 -0.1726685345668031 1101 52-53 -0.18466325473033152 1101 54-55 -0.12523289099218005 1101 56-57 -0.3307848297880369 1101 58-59 -0.14815593221322843 1101 60-61 -0.15936437568327477 1101 62-63 -0.19766103540028013 1101 64-65 -0.08830435517786128 1101 66-67 0.04321568678213339 1101 68-69 0.017752098788886173 1101 70-71 -0.09363952849856005 1101 72-73 -0.04169139931072152 1101 74-75 0.08154520047100533 1101 76-77 -0.11363967835538702 1101 78-79 0.18069824932875278 1101 80-81 0.33866147371289657 1101 82-83 0.004651684077355611 1101 84-85 0.20412344771905921 1101 86-87 0.2973091380872219 1101 88-89 0.35782893655473913 1101 90-91 0.1989397981183778 1101 92-93 0.393849004992191 1101 94-95 0.23952151739119287 1101 96-97 -0.009209753495671436 1101 98-99 0.09804325598916463 1101 100-101 0.5314140744067899 1102 1 -0.01449456652778025 1102 2 -0.003028212780733952 1102 3 0.10781777088482869 1102 4 0.03675562924891551 1102 5 -0.029058912633779244 1102 6 0.08309384014845733 1102 7 0.10146277365382872 1102 8 0.022003742679309823 1102 9 0.14577600692864934 1102 10-11 0.12956498426282792 1102 12-13 0.02087594094688683 1102 14-15 -0.001999940866220129 1102 16-17 0.14883500549909456 1102 18-19 0.1461201705344095 1102 20-21 0.0820494765843307 1102 22-23 0.14510270292601035 1102 24-25 0.06999738238926767 1102 26-27 0.0364049075483166 1102 28-29 0.14592790513905385 1102 30-31 0.2255661302703018 1102 32-33 0.33913072949287226 1102 34-35 0.21642808767034438 1102 36-37 0.1298389609536983 1102 38-39 0.05354920861491763 1102 40-41 0.03502205205050757 1102 42-43 0.20312229359373646 1102 44-45 0.03187689327335619 1102 46-47 -0.44330267133928913 1102 48-49 -0.006082946854846227 1102 50-51 0.2456614150733003 1102 52-53 0.11867111128860586 1102 54-55 0.05567714671182955 1102 56-57 -0.08654589710156557 1102 58-59 -0.08561967835713347 1102 60-61 -0.15739842794156544 1102 62-63 -0.25625715426020435 1102 64-65 -0.06244217256389817 1102 66-67 -0.09183981457573509 1102 68-69 -0.1929882708686108 1102 70-71 -0.14506562336501005 1102 72-73 -0.17012727612670986 1102 74-75 -0.36571697423303107 1102 76-77 -0.3346975658761515 1102 78-79 0.13694973244104958 1102 80-81 0.08105475554377861 1102 82-83 0.14065637730381653 1102 84-85 0.04863889845336544 1102 86-87 -0.25327487857636655 1102 88-89 0.13800697594619749 1102 90-91 -0.22847998198550457 1102 92-93 -0.3199531676025309 1102 94-95 -0.48680636248330345 1102 96-97 -0.17956827117436802 1102 98-99 0.030228770266592875 1102 100-101 0.2930153083562175 1103 1 0.08226641430514547 1103 2 0.08043385783072665 1103 3 -0.01666338294519676 1103 4 0.10195528222266859 1103 5 0.05730901904462371 1103 6 0.07527768665706702 1103 7 0.11339583241930029 1103 8 0.018909210277826105 1103 9 -0.01919691792856071 1103 10-11 -0.056198876629956374 1103 12-13 0.11500519854937608 1103 14-15 -0.06452916879731418 1103 16-17 -0.025995593443695952 1103 18-19 0.039501515615889105 1103 20-21 0.04979635383185865 1103 22-23 -0.20488665407562934 1103 24-25 -0.11756127504487068 1103 26-27 -0.01764781722052078 1103 28-29 -0.11233412548052968 1103 30-31 -0.1620180058568721 1103 32-33 -0.14823877283406972 1103 34-35 -0.08267886801285584 1103 36-37 -0.29638812837975337 1103 38-39 0.029001777931476624 1103 40-41 0.12253329976468308 1103 42-43 -0.06903771904885758 1103 44-45 -0.3037081329954958 1103 46-47 -0.3282889483217062 1103 48-49 -0.02810427800367421 1103 50-51 -0.19972226501752743 1103 52-53 8.392094341758138E-5 1103 54-55 -0.018566636003299664 1103 56-57 -0.06640644157155151 1103 58-59 -0.2500144948944154 1103 60-61 -0.039222444351132424 1103 62-63 0.0422555673479863 1103 64-65 0.07264811553884698 1103 66-67 -0.13037150007190945 1103 68-69 -0.2750353064710538 1103 70-71 -0.46883047115401055 1103 72-73 -0.3127982816965478 1103 74-75 -0.11357950539605355 1103 76-77 -0.3113579542488374 1103 78-79 -0.23987610617884414 1103 80-81 -0.15758142484536464 1103 82-83 -0.12301826301121466 1103 84-85 -0.1681992924949256 1103 86-87 -0.17756768358696107 1103 88-89 -0.14728101391392556 1103 90-91 -0.1599543920247548 1103 92-93 -0.038745800067548686 1103 94-95 -0.12361484979072301 1103 96-97 -0.22279658284006487 1103 98-99 -0.22583773879769709 1103 100-101 0.3449337894390929 1104 1 0.0176941003317026 1104 2 0.07089198012378972 1104 3 -0.015038619889814697 1104 4 0.07401063953434317 1104 5 0.05115927485667271 1104 6 0.0251071185828593 1104 7 -0.15436319631208306 1104 8 0.0031438811831918656 1104 9 0.06490299698769775 1104 10-11 0.09057298837048933 1104 12-13 -0.018892742616259284 1104 14-15 -0.015448986580302915 1104 16-17 0.1627308903543394 1104 18-19 0.13881475679385602 1104 20-21 0.06646860194952353 1104 22-23 0.12954213916125923 1104 24-25 0.17238041615080846 1104 26-27 0.09250759280782006 1104 28-29 0.12640509684442236 1104 30-31 -0.014906446805390772 1104 32-33 -0.29323846037963364 1104 34-35 0.0793183407275393 1104 36-37 0.1467627903163944 1104 38-39 0.35020733929992787 1104 40-41 0.07475661335196548 1104 42-43 0.23758984007312733 1104 44-45 0.26341268752587155 1104 46-47 0.3621760639921945 1104 48-49 0.2452957550043351 1104 50-51 0.3215022038499953 1104 52-53 0.04061887498056649 1104 54-55 0.05534486087776713 1104 56-57 0.18593336143793948 1104 58-59 0.1381500516220342 1104 60-61 0.2081517366577259 1104 62-63 0.10294383236062998 1104 64-65 0.22543272547553528 1104 66-67 0.127661382953157 1104 68-69 0.23137161175080578 1104 70-71 0.1918608382864413 1104 72-73 0.04274201611258732 1104 74-75 0.20864193346462656 1104 76-77 0.11136245030754921 1104 78-79 0.24193824461294966 1104 80-81 0.0939700315928782 1104 82-83 -0.007543917122557531 1104 84-85 0.09654655386401956 1104 86-87 0.2089729010393171 1104 88-89 0.2877508964965756 1104 90-91 0.12668970314726025 1104 92-93 0.3527379739333618 1104 94-95 0.2300186748074573 1104 96-97 0.18246242586219452 1104 98-99 0.2838034833958609 1104 100-101 0.5467240732278356 1105 1 0.0012590228976563367 1105 2 0.052307180093592365 1105 3 0.06601394216436063 1105 4 0.026436822432174267 1105 5 0.2047095409379196 1105 6 0.1609343607734104 1105 7 0.1626729228017325 1105 8 0.1049466917056705 1105 9 0.12750285608414913 1105 10-11 0.16128423675073833 1105 12-13 0.10348811840503913 1105 14-15 0.14114501621260445 1105 16-17 -0.04402697571181591 1105 18-19 -0.1679846254950732 1105 20-21 0.0419960466889151 1105 22-23 0.013567986155102574 1105 24-25 0.10154729249536842 1105 26-27 0.03590321095921922 1105 28-29 0.2927160973853873 1105 30-31 0.07815279558645472 1105 32-33 0.1417202435593694 1105 34-35 0.23201233471397842 1105 36-37 0.06295663333475687 1105 38-39 -0.12164418988093928 1105 40-41 -0.019792652565733704 1105 42-43 0.04539331737130681 1105 44-45 -0.0107752299952395 1105 46-47 0.07395125993619445 1105 48-49 0.026147498056658947 1105 50-51 0.10193721209033413 1105 52-53 -0.07232019052700167 1105 54-55 0.007830801975053703 1105 56-57 -0.029870008924511637 1105 58-59 0.02014176095815401 1105 60-61 -0.0667084606072379 1105 62-63 0.08197813985744773 1105 64-65 0.048695491714042305 1105 66-67 -0.034678056200412755 1105 68-69 -0.011861558453126975 1105 70-71 -0.03833560893134447 1105 72-73 -0.05756998459192886 1105 74-75 -0.05815955608712642 1105 76-77 -0.010247058165532508 1105 78-79 -0.13154657051405394 1105 80-81 -0.20699663591249973 1105 82-83 -0.08396600501114548 1105 84-85 -0.046463466642475026 1105 86-87 0.030639494318723592 1105 88-89 0.3555961101793166 1105 90-91 0.4681740024666503 1105 92-93 0.22425627252306768 1105 94-95 0.3200436159928053 1105 96-97 0.1161921552519054 1105 98-99 0.5693737653287592 1105 100-101 0.7424927209312315 1106 1 -0.007699895382231148 1106 2 -0.03863661577852895 1106 3 -0.05689256490425976 1106 4 0.008654447179722524 1106 5 0.030617131696999422 1106 6 -0.0069514576591771515 1106 7 0.07400733982552765 1106 8 0.09425756309131117 1106 9 -0.10627257369763043 1106 10-11 -0.09260103983663015 1106 12-13 9.111825726648703E-5 1106 14-15 -0.008431975068610598 1106 16-17 -0.04989799191791633 1106 18-19 -0.26942359401878235 1106 20-21 -0.2054548178005149 1106 22-23 -0.010473317228971268 1106 24-25 -0.15508723289746484 1106 26-27 -0.141738900266823 1106 28-29 0.02577419797156466 1106 30-31 0.2694611055219198 1106 32-33 0.06553865151956728 1106 34-35 0.014458396484336333 1106 36-37 0.13917209070138625 1106 38-39 0.37266335654534544 1106 40-41 0.5011822994070769 1106 42-43 0.5194832636793691 1106 44-45 0.4084467295141465 1106 46-47 0.30285623998643985 1106 48-49 0.36279039742121455 1106 50-51 0.03996674265533784 1106 52-53 0.2354879223738422 1106 54-55 0.24442013254990513 1106 56-57 0.0980824318419522 1106 58-59 -0.04486119456127824 1106 60-61 0.20818255461369262 1106 62-63 0.21755027916092473 1106 64-65 0.28613145545967456 1106 66-67 0.22580788045295463 1106 68-69 0.1631975519355251 1106 70-71 0.14536839579748673 1106 72-73 0.2803998198512012 1106 74-75 0.5533004705125499 1106 76-77 0.3518902749707671 1106 78-79 0.21450473237742784 1106 80-81 0.25085950417912173 1106 82-83 0.46069620374704456 1106 84-85 0.13146803919467231 1106 86-87 0.2325107075594488 1106 88-89 0.40919260788879086 1106 90-91 0.3134125375141821 1106 92-93 0.48974196288314786 1106 94-95 0.6973002782261943 1106 96-97 0.7730932931268875 1106 98-99 0.7757244377094281 1106 100-101 1.1852326105918394 1107 1 -0.03364375869533731 1107 2 -0.12818285322819634 1107 3 -0.12483241931135325 1107 4 -0.07421528137893318 1107 5 0.001867634310585231 1107 6 0.008818606375292859 1107 7 -0.05263778197200253 1107 8 0.07613946840191232 1107 9 -0.042260606993160366 1107 10-11 0.02756861850615877 1107 12-13 0.05501253049642685 1107 14-15 -0.07026694977289338 1107 16-17 0.04979878955469985 1107 18-19 0.006983874069000251 1107 20-21 0.062189169740662464 1107 22-23 -0.08808660783264344 1107 24-25 -0.0640083990810254 1107 26-27 -0.02507103332889926 1107 28-29 -0.005972976889950132 1107 30-31 -0.14713354486907093 1107 32-33 0.08902120857746354 1107 34-35 0.028357537690247625 1107 36-37 0.15167141554879748 1107 38-39 0.08722534755531086 1107 40-41 0.2698210117560649 1107 42-43 0.26643232416704166 1107 44-45 0.34050837544612733 1107 46-47 0.4903168560856699 1107 48-49 -0.25849626336412257 1107 50-51 -0.02749300324724402 1107 52-53 0.15158446019139404 1107 54-55 0.10988922856559924 1107 56-57 0.09638179749859432 1107 58-59 -0.12807600110755857 1107 60-61 -0.20882543919291408 1107 62-63 -0.41319676967210484 1107 64-65 -0.2342866590342041 1107 66-67 -0.24367822340651912 1107 68-69 -0.09801898300137069 1107 70-71 -0.1115136595470183 1107 72-73 -0.1751158766962213 1107 74-75 -0.16729438392455265 1107 76-77 -0.1443926349668807 1107 78-79 0.004843598961276285 1107 80-81 0.011506660436435112 1107 82-83 0.12394209731899508 1107 84-85 -0.11467683954792207 1107 86-87 -0.2695395057887673 1107 88-89 0.0430438582713748 1107 90-91 0.3478460694924088 1107 92-93 0.30855651497192227 1107 94-95 0.3632880414606845 1107 96-97 0.21853871379195766 1107 98-99 0.1878825854508186 1107 100-101 0.9476434154337348 1108 1 -0.1423777939456201 1108 2 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-1.2941271410719963 2316 56-57 -1.4793295840028904 2316 58-59 -1.2371912525629796 2316 60-61 -1.212409912620565 2316 62-63 -1.3950448371655284 2316 64-65 -1.3826015528255766 2316 66-67 -1.3781155592913805 2316 68-69 -1.3620794099086027 2316 70-71 -1.3676857142715484 2316 72-73 -1.3834816966422565 2316 74-75 -1.2917947327916082 2316 76-77 -1.2320438257543778 2316 78-79 -1.1701261839699058 2316 80-81 -1.2238430320905813 2316 82-83 -1.4652907446279606 2316 84-85 -1.4580487872624524 2316 86-87 -1.704065775658826 2316 88-89 -2.1004917376164514 2316 90-91 -2.507688625004892 2316 92-93 -2.3251806766663563 2316 94-95 -2.4398641742367673 2316 96-97 -2.3918406535234773 2316 98-99 -2.4593091853838978 2316 100-101 -2.9831152107410546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 15.0 5 49.0 6 104.0 7 270.0 8 562.0 9 1014.0 10 1657.0 11 2634.0 12 3622.0 13 5134.0 14 5670.0 15 7241.0 16 8962.0 17 12047.0 18 11283.0 19 7104.0 20 6298.0 21 6523.0 22 6712.0 23 7087.0 24 8071.0 25 9340.0 26 10618.0 27 12504.0 28 15020.0 29 18016.0 30 21463.0 31 25980.0 32 31514.0 33 39205.0 34 51476.0 35 72073.0 36 103383.0 37 163032.0 38 148500.0 39 16038.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16800536194951 20.543626173673164 15.195296585819268 22.093071878558057 2 15.963248158242385 22.509193911190984 38.58693452983112 22.940623400735515 3 18.185615843281006 26.488818537781324 32.06050724206467 23.265058376873 4 12.917415469573806 18.83055829951323 36.875022315318425 31.377003915594535 5 12.804827249681635 37.957940087833094 34.41355343179844 14.823679230686837 6 29.463599252585603 37.45783892505624 17.843566642466943 15.23499517989122 7 25.607512228794498 35.08729752567749 21.773562000880712 17.531628244647298 8 25.464218130749916 34.27597205527058 21.154921866631756 19.104887947347752 9 26.121895195363177 16.54201825690585 21.52303535936589 35.813051188365094 10-11 23.88875664996489 27.34513169013246 27.96853242564536 20.797579234257288 12-13 25.316877521630982 25.50117229806125 28.245658926722445 20.936291253585328 14-15 22.268446503147363 26.549253951610694 27.590579688584153 23.591719856657782 16-17 20.809393371534124 29.6524259723319 28.20266850112978 21.3355121550042 18-19 20.530796402881123 27.273430545042537 31.650040227176433 20.545732824899908 20-21 21.48881853778132 26.580757649691154 31.659843138188354 20.27058067433917 22-23 21.07411066017638 26.96297442366971 31.427585304023896 20.535329612130013 24-25 21.044832962403152 27.049438844126012 31.632231650857502 20.27349654261333 26-27 20.861527548756083 27.318223554664 31.393305511536447 20.426943385043472 28-29 20.45666067624341 27.32721992787689 31.69382192970972 20.522297466169977 30-31 20.77216952501101 27.088178236911318 31.572188567416067 20.567463670661603 32-33 20.309558097187676 27.287587922354597 31.48840198517073 20.914451995287003 34-35 20.965218452163096 27.205425076398004 31.066477419815573 20.76287905162333 36-37 21.11906674999256 27.067047585037052 30.94682022438473 20.867065440585662 38-39 20.79761177116854 27.059034983671985 31.14719852687799 20.996154718281485 40-41 20.95788213281661 26.931642080255568 31.268880197484783 20.84159558944304 42-43 20.99718399040224 27.193178734485763 30.76770182195583 21.041935453156167 44-45 20.769259608157718 27.53881319026601 30.440288475328504 21.25163872624777 46-47 21.162400567953586 27.345501330322975 29.758887515554157 21.73321058616928 48-49 21.250397213140705 27.594977083628393 29.461968106313876 21.69265759691703 50-51 20.9863304553361 27.970705483230397 29.185617432463072 21.857346628970436 52-53 21.166763862275808 27.98215964676339 29.22556918938862 21.62550730157219 54-55 20.741344631827 27.916998916963216 29.180760029991788 22.160896421218 56-57 20.91177415707604 28.3046308748795 28.722849695916597 22.060745272127868 58-59 20.84440175761817 27.880884712244768 29.020456352323414 22.254257177813642 60-61 20.82509894653406 28.079928447864948 29.068233387068844 22.026739218532146 62-63 20.586387060685766 28.076895611915788 29.08888042559775 22.247836901800696 64-65 20.38180022136796 27.988586458469705 28.9508824964593 22.678730823703035 66-67 20.43523487496623 28.252835888042675 28.582097634766424 22.729831602224674 68-69 20.508616790840495 27.9282211801671 28.670348242127062 22.892813786865347 70-71 20.460835800467144 27.889135189014684 28.455480012496835 23.194548998021332 72-73 20.56246503933447 27.698427811432584 28.394725253799557 23.344381895433393 74-75 20.350380253025957 27.754662413862867 28.261428418409245 23.633528914701927 76-77 20.281710107944996 27.60781692833887 28.54865744827611 23.561815515440028 78-79 20.254652779762075 27.507357773523445 28.417019077825717 23.820970368888762 80-81 20.144603263392167 27.622615236304345 28.305940040227078 23.92684146007641 82-83 20.232852909322446 27.436118681789512 28.176927745974318 24.154100662913727 84-85 20.044987681944228 27.04670149839925 28.117955797817263 24.790355021839257 86-87 20.228531311161596 26.911817642192894 28.15513381948732 24.704517227158192 88-89 20.131926893299585 26.69274634941162 28.110411155035 25.06491560225379 90-91 19.90118573536898 26.77103891042921 28.258086770119874 25.069688584081938 92-93 20.0790854884972 26.46549159158802 28.171393547004985 25.2840293729098 94-95 19.727277055092056 26.192590124132675 28.267795722599764 25.812337098175497 96-97 19.813503445485164 25.879521083512845 28.237565904573746 26.06940956642824 98-99 19.699367135686465 25.8115807921069 28.146662659815348 26.342389412391288 100-101 19.64252347293516 25.475974830491428 28.548135435473675 26.333366261099734 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1746.0 1 1187.0 2 718.5 3 789.0 4 712.0 5 699.5 6 1011.5 7 1715.5 8 2376.5 9 2641.5 10 2286.0 11 1804.0 12 1773.5 13 1861.5 14 1980.5 15 2077.0 16 2151.5 17 2246.0 18 2309.5 19 2493.5 20 2846.0 21 3175.5 22 3531.0 23 4034.0 24 4621.5 25 5443.0 26 6554.0 27 7595.0 28 8527.0 29 9562.0 30 10761.0 31 12201.5 32 14061.0 33 15625.5 34 17078.0 35 18481.0 36 19945.5 37 21501.5 38 22065.5 39 21778.5 40 22089.0 41 23878.0 42 25864.5 43 27376.0 44 28118.5 45 28951.5 46 29388.5 47 29956.5 48 32210.0 49 33051.5 50 31852.5 51 30455.0 52 29068.5 53 28337.0 54 26938.5 55 23928.5 56 20996.0 57 18370.0 58 16131.5 59 14136.0 60 12010.0 61 9886.0 62 8187.5 63 6450.0 64 4694.0 65 3515.5 66 2538.0 67 2031.0 68 1504.0 69 935.5 70 783.5 71 715.0 72 657.5 73 573.5 74 451.5 75 393.5 76 273.0 77 141.0 78 91.5 79 65.0 80 44.0 81 26.5 82 21.0 83 17.0 84 9.0 85 6.5 86 6.5 87 4.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3833474167787392 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.570450947954727E-4 16-17 0.002439808147769063 18-19 2.3803006319698177E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7852254739773635E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006843364316913227 36-37 0.01844732989776609 38-39 0.013032145960034753 40-41 0.007616962022303417 42-43 0.00404651107434869 44-45 0.0023207931161705725 46-47 0.002439808147769063 48-49 1.1901503159849088E-4 50-51 5.35567642193209E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.3803006319698177E-4 60-61 4.165526105947181E-4 62-63 0.0 64-65 0.0 66-67 0.002023255537174345 68-69 0.0011901503159849089 70-71 0.0020827630529735904 72-73 0.0 74-75 0.0 76-77 0.09771134094236102 78-79 0.03594253954274425 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15043499994049248 88-89 0.33282553586517977 90-91 0.2851005081941849 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.975375789962272E-4 100-101 8.331052211894362E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 840230.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.62673946506298 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81909742809458 50.21051842504758 2 10.587434409058275 12.837632550428385 3 2.786123977725696 5.067408375448222 4 1.1210253623953992 2.7185645031869474 5 0.6042382815462989 1.8316498435062418 6 0.35763949315528304 1.3009509824365513 7 0.25356812450997845 1.0761106034917782 8 0.1909412793182568 0.926091775548302 9 0.14772012408137652 0.8060210528785552 >10 0.9913430707748331 11.785877663927842 >50 0.09450070106355579 3.84866122208933 >100 0.043824369686999175 4.482974015638548 >500 9.782225344328883E-4 0.4071980358110695 >1k 0.001173867041319466 1.3526613458751637 >5k 3.912890137731554E-4 1.3476796046855148 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6015 0.7158754150649227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5347 0.6363733739571308 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3639 0.4330956999869084 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2343 0.27885221903526414 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2094 0.24921747616723994 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1201 0.14293705294978756 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1080 0.12853623412637016 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1047 0.12460873808361997 RNA PCR Primer, Index 27 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 975 0.11603965580852862 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1901503159849088E-4 0.0 14-15 0.0 0.0 0.0 1.1901503159849088E-4 0.0 16-17 0.0 0.0 0.0 1.1901503159849088E-4 0.0 18-19 0.0 0.0 0.0 1.1901503159849088E-4 0.0 20-21 0.0 0.0 0.0 5.950751579924544E-4 0.0 22-23 0.0 0.0 0.0 0.002142270568772836 0.0 24-25 0.0 0.0 0.0 0.00470109374814039 0.0 26-27 0.0 0.0 0.0 0.009342679980481536 0.0 28-29 0.0 0.0 0.0 0.02624281446746724 0.0 30-31 0.0 0.0 0.0 0.06682694024255263 0.0 32-33 0.0 0.0 0.0 0.14793568427692416 0.0 34-35 0.0 0.0 0.0 0.268378896254597 0.0 36-37 0.0 0.0 0.0 0.45404234554824274 0.0 38-39 0.0 0.0 0.0 0.7162324601597181 0.0 40-41 0.0 0.0 0.0 1.0078787950918202 0.0 42-43 0.0 0.0 0.0 1.2785189769467884 0.0 44-45 0.0 0.0 0.0 1.5871249538816752 0.0 46-47 0.0 0.0 0.0 1.945836259119527 0.0 48-49 0.0 0.0 0.0 2.3257322399819094 0.0 50-51 0.0 0.0 0.0 2.7362150839651047 0.0 52-53 0.0 0.0 0.0 3.127715030408341 0.0 54-55 0.0 0.0 0.0 3.559263534984469 0.0 56-57 0.0 0.0 0.0 4.052699855991811 0.0 58-59 0.0 0.0 0.0 4.5832688668578845 0.0 60-61 0.0 0.0 0.0 5.162872070742535 0.0 62-63 0.0 0.0 0.0 5.723670899634624 0.0 64-65 0.0 0.0 0.0 6.3091653475833995 0.0 66-67 0.0 0.0 0.0 6.951489473120455 0.0 68-69 0.0 0.0 0.0 7.666769813027385 0.0 70-71 0.0 0.0 0.0 8.4542922771146 0.0 72-73 0.0 0.0 0.0 9.258298323078204 0.0 74-75 0.0 0.0 0.0 10.081822834223962 0.0 76-77 0.0 0.0 0.0 10.940575794722875 0.0 78-79 0.0 0.0 0.0 11.865025052664151 0.0 80-81 0.0 0.0 0.0 12.852433262321032 0.0 82-83 0.0 0.0 0.0 13.851326422527166 0.0 84-85 0.0 0.0 0.0 14.865870059388502 0.0 86-87 0.0 0.0 0.0 15.883686609618795 0.0 88-89 0.0 0.0 0.0 16.926555824000573 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3620 0.0 46.31843 1 GTATCAA 5815 0.0 34.896324 1 TATCAAC 7335 0.0 27.557898 2 ATCAACG 7445 0.0 27.02326 3 TCAACGC 7505 0.0 26.743994 4 CAACGCA 7665 0.0 26.185738 5 AACGCAG 7805 0.0 25.716038 6 ACGCAGA 8855 0.0 22.505947 7 CGCAGAG 8825 0.0 22.421154 8 GAGTACT 5620 0.0 21.614897 12-13 GCAGAGT 9155 0.0 21.457472 9 GTACATG 4260 0.0 21.35716 1 TACATGG 4275 0.0 20.866919 2 GTAATAC 160 5.027822E-5 20.759392 3 TAAGGCG 325 2.1827873E-11 20.44002 5 ACATGGG 4315 0.0 20.233622 3 GTACTTT 6190 0.0 19.510103 14-15 CAGAGTA 9070 0.0 19.487476 10-11 AGTACTT 5650 0.0 19.358513 12-13 AGAGTAC 8415 0.0 18.551472 10-11 >>END_MODULE