FastQCFastQC Report
Thu 26 May 2016
SRR938852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841262
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.799037636313063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.6334530740720489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34510.41021703107949725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23060.27411198889287763No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.15666938480520934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.12671438862090526No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.11589730666546212No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.1010386776057875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36500.044.800551
GTATCAA59550.036.7990761
TATAGCG851.645383E-533.4884645
TATCACG600.003974050731.6279952
TATCAAC72700.029.9530562
ATCAACG73450.029.6472053
ATCACGC650.005878181729.1950683
TCAACGC74850.029.1560654
CAACGCA75850.028.8342235
AACGCAG76750.028.49616
ACAATCG350.0083816527.15015486-87
TCACGCA700.00844046327.1097094
GTACATG41750.026.0733451
ACGCAGA84500.025.7141177
CGCAGAG85400.025.1653638
TACATGG42450.024.4753742
GCAGAGT85650.024.0934459
GAGTACT51300.023.82341212-13
GCACTAT1000.001646105723.7677331
ACATGGG42050.023.0158523