FastQCFastQC Report
Thu 26 May 2016
SRR938852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841262
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60750.7221293723001871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.6342851572993907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.4202020298075986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23530.27969883341931523No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT19290.22929836364889894No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.13967111316094152No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.13860129186864498No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10210.12136528215942238RNA PCR Primer, Index 27 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.1169671279577587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.044.8737641
GTATCAA56800.038.4290471
TATCAAC72900.029.7494752
ATCAACG73050.029.6234253
TCAACGC73400.029.5468224
CAACGCA75050.028.9604575
AACGCAG76400.028.4402686
TCGTATC350.00844295727.10962794-95
ACGCAGA85950.025.0594487
CGCAGAG87150.024.7143978
GCAGAGT89400.023.933199
GTACATG41650.023.2921961
TACATGG42050.023.0226232
GAGTACT55100.022.1293612-13
AGTACTT56300.021.91050112-13
ACATGGG42350.021.8510273
ATACGTC2658.021743E-1021.4894835
CATGGGA23600.020.9127884
AGAGTAC81250.020.70047210-11
GTACTTT61300.020.0459514-15
Produced by FastQC (version 0.11.5)