##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938852_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 841262 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.994886254222823 31.0 31.0 34.0 30.0 34.0 2 32.19455175676543 33.0 31.0 34.0 30.0 34.0 3 32.25092539541784 34.0 31.0 34.0 30.0 34.0 4 35.910736488751425 37.0 35.0 37.0 35.0 37.0 5 35.81618924900923 37.0 35.0 37.0 35.0 37.0 6 35.84625598208406 37.0 35.0 37.0 35.0 37.0 7 35.75522726570319 37.0 35.0 37.0 33.0 37.0 8 35.751833554825964 37.0 35.0 37.0 33.0 37.0 9 37.373246384598374 39.0 37.0 39.0 34.0 39.0 10-11 37.312262410521335 39.0 37.0 39.0 34.0 39.0 12-13 37.22972272609485 39.0 37.0 39.0 33.0 39.0 14-15 38.4144327213163 40.0 38.0 41.0 33.0 41.0 16-17 38.345316322382324 40.0 38.0 41.0 33.0 41.0 18-19 38.48810358722966 40.0 38.0 41.0 33.5 41.0 20-21 38.4899454628879 40.0 38.0 41.0 34.0 41.0 22-23 38.3918618694295 40.0 38.0 41.0 34.0 41.0 24-25 38.29539727219344 40.0 38.0 41.0 33.0 41.0 26-27 38.087629656397176 40.0 37.0 41.0 33.0 41.0 28-29 37.88319393958125 40.0 37.0 41.0 33.0 41.0 30-31 37.58075011114255 40.0 36.5 41.0 32.5 41.0 32-33 37.32959232676622 39.5 36.0 41.0 32.0 41.0 34-35 37.209646935199736 40.0 36.0 41.0 31.5 41.0 36-37 37.027540171789525 40.0 35.0 41.0 31.0 41.0 38-39 36.846374851116536 40.0 35.0 41.0 31.0 41.0 40-41 36.602031828372134 39.0 35.0 41.0 30.0 41.0 42-43 36.32518109697098 39.0 35.0 41.0 30.0 41.0 44-45 35.9857422538995 39.0 35.0 41.0 29.5 41.0 46-47 35.51912602732561 39.0 35.0 41.0 27.0 41.0 48-49 35.07210773813628 39.0 35.0 41.0 25.0 41.0 50-51 34.76523960430876 39.0 34.5 40.0 24.0 41.0 52-53 34.509378766662465 38.0 34.0 40.0 23.0 41.0 54-55 34.21552203712993 38.0 34.0 40.0 22.5 41.0 56-57 33.92213067985954 38.0 34.0 40.0 21.5 41.0 58-59 33.58847124914712 37.5 33.0 40.0 19.5 41.0 60-61 33.196428104443086 37.0 33.0 40.0 17.5 41.0 62-63 32.81993124615161 36.5 32.5 40.0 14.0 41.0 64-65 32.40223794727445 36.0 32.0 39.0 10.0 41.0 66-67 31.970883624839825 35.5 31.5 39.0 7.5 40.0 68-69 31.63427029867033 35.0 31.0 38.5 4.0 40.0 70-71 31.21289681454767 35.0 31.0 38.0 2.0 40.0 72-73 30.737167493598903 35.0 30.5 37.0 2.0 39.5 74-75 30.264978686782477 35.0 30.0 37.0 2.0 39.0 76-77 28.339041820503006 32.5 27.0 35.0 2.0 37.0 78-79 29.244017915940574 34.0 29.0 35.5 2.0 37.5 80-81 29.281964477178335 34.0 29.0 35.0 2.0 37.0 82-83 28.974636320195135 34.0 29.0 35.0 2.0 37.0 84-85 28.581866291357507 34.0 29.0 35.0 2.0 36.0 86-87 28.015882091429305 34.0 28.0 35.0 2.0 36.0 88-89 27.435561691839165 33.5 26.5 35.0 2.0 35.5 90-91 26.935689476049077 33.0 25.5 35.0 2.0 35.0 92-93 26.64669032952873 33.0 25.0 35.0 2.0 35.0 94-95 26.323343976074042 33.0 25.0 35.0 2.0 35.0 96-97 25.754601420247198 33.0 23.5 35.0 2.0 35.0 98-99 24.99023134291101 32.5 12.5 34.5 2.0 35.0 100-101 21.729382760661956 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06775466270079988 1101 2 0.1341173821808468 1101 3 -0.05874648852836373 1101 4 0.11485911346946409 1101 5 -0.06491512113811382 1101 6 -0.037821298100645606 1101 7 0.08312025204478601 1101 8 0.11689734895304582 1101 9 0.14271378817800695 1101 10-11 0.10642585640126612 1101 12-13 0.11382052910389717 1101 14-15 0.20559422920426584 1101 16-17 0.3601786502455866 1101 18-19 0.17999203647485018 1101 20-21 0.16871636853935712 1101 22-23 0.20425321225410187 1101 24-25 0.24077428859845185 1101 26-27 0.27145287772963655 1101 28-29 0.24589321553531107 1101 30-31 0.26586750011484384 1101 32-33 0.2651991297752119 1101 34-35 0.5504692318091884 1101 36-37 0.46724801042711306 1101 38-39 0.37983170439580505 1101 40-41 0.672523645089818 1101 42-43 0.6925812449873376 1101 44-45 0.5725092882459606 1101 46-47 0.5890669484149456 1101 48-49 0.460574784679892 1101 50-51 0.3216750525836858 1101 52-53 0.3833856139321483 1101 54-55 0.4936495217748913 1101 56-57 0.40042227058572877 1101 58-59 0.41969429130485736 1101 60-61 0.4007843104900388 1101 62-63 0.2977879698616164 1101 64-65 0.31591431745761867 1101 66-67 0.1849453363293989 1101 68-69 0.39847427461789664 1101 70-71 0.2074129994299021 1101 72-73 0.07699169046787446 1101 74-75 0.20480188142371603 1101 76-77 0.08115456558290646 1101 78-79 -0.1413043228138271 1101 80-81 0.24028464026459773 1101 82-83 0.11559491983096493 1101 84-85 0.07471855219256796 1101 86-87 0.12950352959422773 1101 88-89 -0.08627123456372843 1101 90-91 -0.1856477685383453 1101 92-93 -0.25689030664217327 1101 94-95 -0.20158179721919112 1101 96-97 -0.11932245872587899 1101 98-99 -0.2060130447591213 1101 100-101 0.28242914090020577 1102 1 0.16841349864925803 1102 2 0.19367343570876017 1102 3 0.07744998008625714 1102 4 0.09812816824282322 1102 5 -0.13796118409560165 1102 6 -0.034487764137956844 1102 7 -0.006389764761784988 1102 8 0.08578590860400936 1102 9 0.19101299137079764 1102 10-11 0.04303546716487716 1102 12-13 0.11893503445220688 1102 14-15 0.07782187870785862 1102 16-17 0.12257252888951342 1102 18-19 0.22002762503650075 1102 20-21 0.2969200721573131 1102 22-23 0.10594173980436494 1102 24-25 0.24741126212000353 1102 26-27 0.16127726530852016 1102 28-29 0.1967058416038583 1102 30-31 -0.0018091038782941382 1102 32-33 -0.05959510938424728 1102 34-35 0.1141374155424657 1102 36-37 -0.028708536340360524 1102 38-39 -0.10376916047106732 1102 40-41 0.09605453660951468 1102 42-43 -0.16031576578954798 1102 44-45 -0.2521491402705678 1102 46-47 -0.22977708695137977 1102 48-49 0.04787402028203758 1102 50-51 0.0014922020726260143 1102 52-53 -0.32284670560560613 1102 54-55 -0.3406013050495531 1102 56-57 -0.5181553907241394 1102 58-59 -0.42696623301682024 1102 60-61 -0.37548848987912464 1102 62-63 -0.4979821459323901 1102 64-65 -0.31655507499039004 1102 66-67 -0.2878400627918509 1102 68-69 -0.22840913348845504 1102 70-71 -0.35937574248512405 1102 72-73 -0.2876359645916118 1102 74-75 -0.5049916092339011 1102 76-77 -0.499090233100965 1102 78-79 -0.5392125302522395 1102 80-81 -0.2987493448054934 1102 82-83 -0.09658143028887167 1102 84-85 -0.0950146570592274 1102 86-87 -0.015283733836749747 1102 88-89 -0.3174078307477579 1102 90-91 -0.2353506055918082 1102 92-93 -0.14430251200638722 1102 94-95 -0.08763203789725793 1102 96-97 0.03697398504156979 1102 98-99 0.27050483208840603 1102 100-101 0.8985619019768762 1103 1 0.2798228401265419 1103 2 0.23151962101658086 1103 3 0.1621644458128273 1103 4 0.09677786134122357 1103 5 -0.10679435653490543 1103 6 0.03376438537620885 1103 7 0.10014313430804123 1103 8 0.04995315418845081 1103 9 0.12136692816199712 1103 10-11 0.20454901384068336 1103 12-13 0.12767443724966654 1103 14-15 0.2311170485788736 1103 16-17 0.28215685089410414 1103 18-19 -0.04662565885575276 1103 20-21 0.22600715642669655 1103 22-23 0.19749344994249896 1103 24-25 0.13875817069911278 1103 26-27 0.2831026155913676 1103 28-29 0.2926325160295278 1103 30-31 0.3742077614248345 1103 32-33 0.31018598451471036 1103 34-35 0.3851656637222618 1103 36-37 0.43187468196126133 1103 38-39 0.5520491886659684 1103 40-41 0.6244981951292345 1103 42-43 0.6096418604370797 1103 44-45 0.6653505665113357 1103 46-47 0.6115971886495686 1103 48-49 0.7808651154741639 1103 50-51 0.8680930074449833 1103 52-53 0.7905903517478521 1103 54-55 0.7191106796008526 1103 56-57 0.7576673491658497 1103 58-59 0.8148627561777104 1103 60-61 0.7609000255952765 1103 62-63 0.7869243400517831 1103 64-65 0.8346605019575577 1103 66-67 0.6952322312562771 1103 68-69 0.6317581662285505 1103 70-71 0.7358524714107055 1103 72-73 0.8657456670242176 1103 74-75 0.8120906752808743 1103 76-77 1.0143361481066115 1103 78-79 0.5074971116608076 1103 80-81 0.683291423034948 1103 82-83 0.723442809073326 1103 84-85 0.6913631822692885 1103 86-87 0.51982650112868 1103 88-89 0.4223859241580037 1103 90-91 0.627381224660482 1103 92-93 0.703815027848723 1103 94-95 0.8918216441701254 1103 96-97 0.8533585608677683 1103 98-99 0.7208351911377164 1103 100-101 1.1792227403701006 1104 1 0.004678734028885856 1104 2 0.048339655098232015 1104 3 0.05916081537642981 1104 4 0.02752098944376513 1104 5 -0.05118940728692678 1104 6 0.04065480546955769 1104 7 0.07572585639442764 1104 8 -0.17039210772160374 1104 9 0.03449280798714227 1104 10-11 0.05574261241815037 1104 12-13 0.007489210903074195 1104 14-15 -0.1212701485760519 1104 16-17 0.12268882096152822 1104 18-19 0.001004925718596894 1104 20-21 0.0070822101423360095 1104 22-23 -0.029531814931175404 1104 24-25 -0.1900526761557373 1104 26-27 -0.18870148902447426 1104 28-29 -0.18484173750829314 1104 30-31 -0.0987832736199934 1104 32-33 -0.13552935963583934 1104 34-35 -0.05715633375866247 1104 36-37 -0.06396068886188289 1104 38-39 0.06265821289357376 1104 40-41 -0.0663205053227145 1104 42-43 -0.017066296931226077 1104 44-45 0.09262107106790296 1104 46-47 -0.08640014409103713 1104 48-49 0.16582325431983236 1104 50-51 -0.09534331173705368 1104 52-53 -0.1712107892464445 1104 54-55 -0.12444788896993941 1104 56-57 -0.20022181138833872 1104 58-59 -0.09525875230565362 1104 60-61 -0.1368728128132588 1104 62-63 -0.06606891770920242 1104 64-65 -0.2831412317610429 1104 66-67 -0.10266730197333374 1104 68-69 0.17799140324418516 1104 70-71 0.05632368723644632 1104 72-73 0.23953836169710385 1104 74-75 0.04057922829265692 1104 76-77 -0.1122312372382801 1104 78-79 0.12066670490374776 1104 80-81 0.1582717890422103 1104 82-83 0.07695988694248257 1104 84-85 0.4145816752677334 1104 86-87 0.5439022216591702 1104 88-89 0.522547441532236 1104 90-91 0.4706071267127854 1104 92-93 0.5671209476827563 1104 94-95 0.5212933786296361 1104 96-97 0.45930877202580334 1104 98-99 0.6300061180103569 1104 100-101 1.4189174873509103 1105 1 0.0777240811167843 1105 2 0.016987839065564003 1105 3 -0.05995373437641405 1105 4 -0.0918127737197878 1105 5 -0.16885542920827135 1105 6 -0.1154810208790309 1105 7 -0.014014096324245884 1105 8 0.046242321678782616 1105 9 -0.04965505360753042 1105 10-11 0.04963042673148976 1105 12-13 -0.046806942116489836 1105 14-15 -0.03646894505143905 1105 16-17 0.08210425186631909 1105 18-19 0.15453383180069835 1105 20-21 0.26625263782339914 1105 22-23 -0.021399427224324086 1105 24-25 0.011009004480406759 1105 26-27 0.16672726661704473 1105 28-29 0.1677329454919132 1105 30-31 0.17105124174643294 1105 32-33 0.05266750260357611 1105 34-35 0.14502075629076927 1105 36-37 0.1880724080648264 1105 38-39 0.29615058340722555 1105 40-41 0.11539451724337368 1105 42-43 0.10179680214464071 1105 44-45 -0.016691200584794785 1105 46-47 0.06917260467319153 1105 48-49 0.24405880127878987 1105 50-51 0.07218301536373417 1105 52-53 0.1417206528339392 1105 54-55 -0.01758350040342549 1105 56-57 -0.03019817072640052 1105 58-59 0.127627557601933 1105 60-61 0.09346460390713673 1105 62-63 0.1700475662796208 1105 64-65 -0.021421910892783558 1105 66-67 0.15259811818570057 1105 68-69 -0.052556630282573735 1105 70-71 0.058021516593850464 1105 72-73 0.21623726563005263 1105 74-75 0.31210512556323167 1105 76-77 0.35869504687949316 1105 78-79 -0.06524560973197069 1105 80-81 -0.010638591357530203 1105 82-83 0.05394677712086349 1105 84-85 0.06384666559655372 1105 86-87 0.20948356702774973 1105 88-89 0.19606560185890842 1105 90-91 0.09622930595198653 1105 92-93 0.46080029434082803 1105 94-95 0.6817232088467016 1105 96-97 0.7335425996638847 1105 98-99 0.8860409342579381 1105 100-101 1.2808689165363845 1106 1 0.10349985067963985 1106 2 0.08986011052766685 1106 3 0.1859781132088969 1106 4 0.08888657057597271 1106 5 0.06291546877827159 1106 6 0.05105047747549918 1106 7 0.20022216133413906 1106 8 0.15936992332397182 1106 9 0.10916909241838368 1106 10-11 0.1853214816236246 1106 12-13 0.13143178719103332 1106 14-15 0.22202252141440226 1106 16-17 0.20174613859527568 1106 18-19 0.1694126906491178 1106 20-21 0.29019949736697015 1106 22-23 0.3177571776787005 1106 24-25 0.2569463691396905 1106 26-27 0.18945416318018005 1106 28-29 0.4450355652744946 1106 30-31 0.1864287061544161 1106 32-33 0.1972686319486172 1106 34-35 0.22518728916839592 1106 36-37 0.09481799647456057 1106 38-39 0.36874286336563245 1106 40-41 0.5279668253940173 1106 42-43 0.5901661205929116 1106 44-45 0.459554347826149 1106 46-47 0.44005790194693617 1106 48-49 0.5139919895130731 1106 50-51 0.6925531558560252 1106 52-53 0.8024337070584977 1106 54-55 0.8916399438506843 1106 56-57 0.7106455553950113 1106 58-59 0.5649611833401806 1106 60-61 0.5678943453494654 1106 62-63 0.6722288793983964 1106 64-65 0.539232800274597 1106 66-67 0.5857277335628979 1106 68-69 0.4402353125826153 1106 70-71 0.5945224779439897 1106 72-73 0.5451555605231739 1106 74-75 0.5903235796239379 1106 76-77 0.6756454290747946 1106 78-79 0.5006179088922913 1106 80-81 0.2132953030622211 1106 82-83 0.5288123674063954 1106 84-85 0.6154430309482244 1106 86-87 0.6813761440805841 1106 88-89 0.5939473213101785 1106 90-91 0.5758382005786338 1106 92-93 0.6717876850682813 1106 94-95 1.1947476412087532 1106 96-97 1.0393809052601313 1106 98-99 1.1423345363831139 1106 100-101 1.8336948997479254 1107 1 -0.0367829415504346 1107 2 -0.007631824402658083 1107 3 -0.03149872116545538 1107 4 0.010159135611317538 1107 5 -0.134362312812776 1107 6 -0.009612574209583613 1107 7 0.01180428776697795 1107 8 0.1011943450413142 1107 9 0.22985822688197288 1107 10-11 0.027828173915473542 1107 12-13 0.13752486152695553 1107 14-15 0.16455872842929864 1107 16-17 0.025273491193253506 1107 18-19 0.17319743908758056 1107 20-21 -0.006785882549287692 1107 22-23 0.19321328310803665 1107 24-25 -0.02470508257285786 1107 26-27 0.16425751275805567 1107 28-29 0.1485450717608714 1107 30-31 0.19043241265452338 1107 32-33 0.1277683230736102 1107 34-35 0.08671175893295668 1107 36-37 0.08858833074596362 1107 38-39 -0.1524754769459875 1107 40-41 -0.2923276354474922 1107 42-43 -0.062306938642450405 1107 44-45 0.07139112454384389 1107 46-47 0.03787793077526658 1107 48-49 0.14992307606671318 1107 50-51 -0.10908662724507678 1107 52-53 -0.28513235787389135 1107 54-55 -0.02760296918384597 1107 56-57 -0.13473161530991007 1107 58-59 -0.28668477726109387 1107 60-61 -0.21173912297368958 1107 62-63 -0.206692803627206 1107 64-65 -0.22239256865944412 1107 66-67 -0.2701539329893734 1107 68-69 -0.3350388997861167 1107 70-71 -0.11415759618601484 1107 72-73 -0.09969514988934591 1107 74-75 -0.11268280379290374 1107 76-77 -0.03253426754130828 1107 78-79 -0.19457393680748325 1107 80-81 0.103120273890692 1107 82-83 -0.06590998828033179 1107 84-85 -0.2683060252670266 1107 86-87 -0.08242576408058255 1107 88-89 -0.07656129287061475 1107 90-91 0.20992976485895554 1107 92-93 0.49478583181823055 1107 94-95 0.7209903483266054 1107 96-97 0.6133729431487964 1107 98-99 0.7686692196146332 1107 100-101 1.1705574160496646 1108 1 0.21636541213066351 1108 2 0.1727307357157244 1108 3 0.16315166729181385 1108 4 0.07470223587029068 1108 5 0.0917494148971798 1108 6 0.0711865378880816 1108 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sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 16.0 5 55.0 6 109.0 7 273.0 8 539.0 9 1029.0 10 1752.0 11 2639.0 12 3754.0 13 5049.0 14 5717.0 15 7321.0 16 9223.0 17 12272.0 18 11668.0 19 7333.0 20 6594.0 21 6698.0 22 6910.0 23 7495.0 24 8531.0 25 9724.0 26 11004.0 27 12836.0 28 15505.0 29 18582.0 30 22328.0 31 26353.0 32 32199.0 33 40061.0 34 52743.0 35 73735.0 36 106118.0 37 163064.0 38 139242.0 39 12787.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.127631182317835 20.55893598186127 15.134235424617154 22.17919741120374 2 15.947469397167588 22.52068915510269 38.57418972924012 22.9576517184896 3 18.138820010888406 26.412223540347718 32.150863821259016 23.298092627504868 4 12.913218474149552 18.801752605014848 36.854511436389615 31.430517484445986 5 12.844381400580474 37.98394125347636 34.32631382448097 14.845363521462197 6 29.368781427195888 37.44398083758306 18.01504939196177 15.172188343259274 7 25.675829884150243 35.0996479099258 21.720700566529807 17.50382163939415 8 25.393278193951467 34.34126348272001 21.16629539905523 19.099162924273294 9 26.120400065615705 16.57153181767392 21.53526487586507 35.7728032408453 10-11 23.973209297460244 27.3134291100751 27.933866025090875 20.77949556737378 12-13 25.365106233254327 25.42971155240579 28.286372140902593 20.91881007343729 14-15 22.287230375317083 26.606871581029452 27.58207312347402 23.523824920179447 16-17 20.689403079995483 29.686651173572187 28.18926934804187 21.43467639839046 18-19 20.526304528196924 27.305940361029023 31.72632307176599 20.441432039008063 20-21 21.5138090155029 26.535966203156686 31.681568881038253 20.268655900302164 22-23 21.123145940265932 26.94130960390461 31.37661037821749 20.55893407761197 24-25 21.084788837702472 27.070746813858488 31.607867004334565 20.23659734410447 26-27 20.862711581334782 27.369482124612706 31.39804898517631 20.369757308876206 28-29 20.50496753686723 27.23824444703315 31.68507551749633 20.57171249860329 30-31 20.652008530041773 27.16817115238772 31.60067850443738 20.579141813133127 32-33 20.333617826551063 27.299224260694054 31.441928911563817 20.925229001191067 34-35 20.95258304535085 27.267869476472217 31.106506561483222 20.67304091669371 36-37 21.123537129143998 27.07465062598716 30.914629035508373 20.887183209360472 38-39 20.81011613848168 27.090855268554638 31.13989045962021 20.959138133343476 40-41 20.893089805894494 26.980582316522266 31.332680291776434 20.793647585806806 42-43 21.0254243030517 27.211921498140164 30.699783524309826 21.062870674498306 44-45 20.753703195190866 27.544093015846975 30.461417942472536 21.24078584648962 46-47 21.12332758203622 27.46691122061623 29.785934066717818 21.623827130629728 48-49 21.195970993147693 27.69130917759832 29.416659385830247 21.69606044342374 50-51 21.021394048465282 27.986584440994598 29.16701336801139 21.825008142528723 52-53 21.293306960257325 27.9382047447763 29.199702351942676 21.5687859430237 54-55 20.71584120048213 27.886734453713586 29.185259764496674 22.212164581307604 56-57 20.95730049652244 28.27622195991446 28.74088708364091 22.02559045992219 58-59 20.852746212965457 27.815528758401847 29.11661516644408 22.215109862188616 60-61 20.87972046176825 27.99180262099782 29.113176965792942 22.01529995144099 62-63 20.57157257345547 28.06820262036141 29.105993642853917 22.254231163329205 64-65 20.40923552588019 27.937483618156993 29.005178594035197 22.648102261927615 66-67 20.40653054625685 28.315081976567253 28.548078020389532 22.730309456786365 68-69 20.50135421625737 27.895487907148965 28.74659068553595 22.856567191057714 70-71 20.40458263894007 27.837403805235468 28.522980950048854 23.235032605775608 72-73 20.57397101022036 27.70759882177015 28.37338427267605 23.34504589533344 74-75 20.29741191014642 27.774492352222712 28.198262719797444 23.72983301783342 76-77 20.320092678439 27.50267170102164 28.575098735484055 23.602136885055305 78-79 20.305132884097358 27.51653685960959 28.40048661279168 23.777843643501374 80-81 20.201033219455717 27.67505414535919 28.187146807028558 23.93676582815654 82-83 20.198726075278884 27.470093912869824 28.072346371537403 24.25883364031389 84-85 20.103903421288493 27.06148619573926 28.110208234771093 24.724402148201154 86-87 20.198883548464256 26.987853740326845 28.06872448208525 24.744538229123652 88-89 20.100373328179867 26.755325399140258 28.046376438154507 25.09792483452537 90-91 19.980224630062143 26.711667468263332 28.242319291667016 25.065788610007505 92-93 20.07242720281578 26.499286485664037 28.116209227009296 25.312077084510886 94-95 19.811294962318428 26.19451774718874 28.09868530537527 25.895501985117562 96-97 19.885362705078798 25.899244230691508 28.113061091550552 26.102331972679142 98-99 19.706048769586644 25.859660842876536 28.06622669275446 26.36806369478236 100-101 19.71162047066725 25.320875718051354 28.66600693608637 26.301496875195024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1715.0 1 1160.5 2 729.0 3 793.0 4 689.5 5 729.5 6 1046.5 7 1684.5 8 2325.0 9 2565.5 10 2214.0 11 1816.5 12 1808.0 13 1869.0 14 1951.5 15 2038.5 16 2144.5 17 2262.5 18 2360.0 19 2475.5 20 2731.0 21 3105.5 22 3562.0 23 3997.0 24 4497.0 25 5356.5 26 6536.5 27 7753.0 28 8629.5 29 9586.5 30 10885.0 31 12196.5 32 14021.5 33 15588.0 34 17073.0 35 18675.5 36 20210.5 37 21568.5 38 22072.5 39 21982.0 40 22161.5 41 23944.0 42 25964.0 43 27483.0 44 28228.0 45 28860.5 46 29395.0 47 30135.5 48 32502.5 49 33163.5 50 31769.0 51 30511.5 52 28956.5 53 27913.5 54 26799.0 55 24219.0 56 21176.5 57 18382.5 58 16194.0 59 14090.5 60 11990.5 61 9882.5 62 8067.5 63 6406.0 64 4672.5 65 3463.5 66 2527.0 67 2067.0 68 1563.0 69 997.0 70 796.5 71 680.0 72 610.5 73 545.5 74 448.5 75 385.5 76 259.0 77 144.0 78 98.0 79 71.0 80 50.0 81 41.5 82 35.5 83 24.0 84 12.0 85 9.5 86 6.5 87 3.0 88 1.5 89 1.5 90 1.0 91 1.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23215122042835645 2 0.0 3 0.0 4 0.0 5 0.02674553230741434 6 0.003803809039276706 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0032094638768897206 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.7830354871609558E-4 26-27 8.91517743580478E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0077264871110308084 36-37 0.017889789387848257 38-39 0.013194462604991072 40-41 0.008320832273417794 42-43 0.007013272916166426 44-45 0.0034472019418445147 46-47 2.971725811934926E-4 48-49 0.001485862905967463 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0013075593572513676 58-59 4.1604161367088974E-4 60-61 0.0020802080683544485 62-63 4.7547612990958825E-4 64-65 0.001248124841012669 66-67 0.005467975493960264 68-69 5.943451623869853E-5 70-71 0.0 72-73 0.0 74-75 5.349106461482867E-4 76-77 0.06008829591732421 78-79 0.13895789896607716 80-81 9.509522598191765E-4 82-83 4.1604161367088974E-4 84-85 0.0 86-87 0.10947837891168267 88-89 0.3555967106561333 90-91 0.39833012783175753 92-93 4.1604161367088974E-4 94-95 2.3773806495479413E-4 96-97 0.0 98-99 0.0 100-101 0.001129255808535272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 841262.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.190941400555644 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49171125191798 49.6525375799575 2 10.805271176744837 13.007588884331225 3 2.807896281210001 5.070297615634478 4 1.138604779079163 2.7413477414378598 5 0.6116593485070565 1.8408176001545138 6 0.3832619336647669 1.3841337954167774 7 0.26255250034658995 1.1062297514051673 8 0.21270849815922918 1.0242499798481888 9 0.15001566921119427 0.8126625919190519 >10 0.9908547142252586 11.689903624480506 >50 0.0988218048040601 4.029267180823697 >100 0.04407277710484279 4.547082372668294 >500 9.881788557780835E-4 0.40660128505121657 >1k 0.0011858146269337004 1.3298407740162521 >5k 3.9527154231123345E-4 1.357439222855305 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6075 0.7221293723001871 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5336 0.6342851572993907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3535 0.4202020298075986 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2353 0.27969883341931523 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 1929 0.22929836364889894 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1175 0.13967111316094152 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1166 0.13860129186864498 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1021 0.12136528215942238 RNA PCR Primer, Index 27 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 984 0.1169671279577587 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1886903247739705E-4 0.0 4 0.0 0.0 0.0 1.1886903247739705E-4 0.0 5 0.0 0.0 0.0 1.1886903247739705E-4 0.0 6 0.0 0.0 0.0 1.1886903247739705E-4 0.0 7 0.0 0.0 0.0 1.1886903247739705E-4 0.0 8 0.0 0.0 0.0 1.1886903247739705E-4 0.0 9 0.0 0.0 0.0 1.1886903247739705E-4 0.0 10-11 0.0 0.0 0.0 1.1886903247739705E-4 0.0 12-13 0.0 0.0 0.0 1.1886903247739705E-4 0.0 14-15 0.0 0.0 0.0 1.1886903247739705E-4 0.0 16-17 0.0 0.0 0.0 1.1886903247739705E-4 0.0 18-19 0.0 0.0 0.0 2.377380649547941E-4 0.0 20-21 0.0 0.0 0.0 5.349106461482868E-4 0.0 22-23 0.0 0.0 0.0 0.0018424700033996544 0.0 24-25 0.0 0.0 0.0 0.004695326782857183 0.0 26-27 0.0 0.0 0.0 0.008320832273417794 0.0 28-29 0.0 0.0 0.0 0.02502193133649208 0.0 30-31 0.0 0.0 0.0 0.06579400947623927 0.0 32-33 0.0 0.0 0.0 0.1381258157387354 0.0 34-35 0.0 0.0 0.0 0.2559844614400746 0.0 36-37 0.0 0.0 0.0 0.43357479596130577 0.0 38-39 0.0 0.0 0.0 0.680346907384382 0.0 40-41 0.0 0.0 0.0 0.9608778240310392 0.0 42-43 0.0 0.0 0.0 1.2206660945103902 0.0 44-45 0.0 0.0 0.0 1.5297850134678614 0.0 46-47 0.0 0.0 0.0 1.8915034792965806 0.0 48-49 0.0 0.0 0.0 2.264692806759369 0.0 50-51 0.0 0.0 0.0 2.6737805820303304 0.0 52-53 0.0 0.0 0.0 3.0653946095271154 0.0 54-55 0.0 0.0 0.0 3.4978401496798854 0.0 56-57 0.0 0.0 0.0 4.008858120300216 0.0 58-59 0.0 0.0 0.0 4.540024391925464 0.0 60-61 0.0 0.0 0.0 5.114696729437441 0.0 62-63 0.0 0.0 0.0 5.681523710805909 0.0 64-65 0.0 0.0 0.0 6.269033903825443 0.0 66-67 0.0 0.0 0.0 6.9198418566391915 0.0 68-69 0.0 0.0 0.0 7.639058937643682 0.0 70-71 0.0 0.0 0.0 8.435362586209767 0.0 72-73 0.0 0.0 0.0 9.232320014454475 0.0 74-75 0.0 0.0 0.0 10.038668096264896 0.0 76-77 0.0 0.0 0.0 10.888225071380855 0.0 78-79 0.0 0.0 0.0 11.783190016903177 0.0 80-81 0.0 0.0 0.0 12.740739508024848 0.0 82-83 0.0 0.0 0.0 13.738288428575164 0.0 84-85 0.0 0.0 0.0 14.737917557193835 0.0 86-87 0.0 0.0 0.0 15.742063709046647 0.0 88-89 0.0 0.0 0.0 16.78811119484774 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3370 0.0 44.873764 1 GTATCAA 5680 0.0 38.429047 1 TATCAAC 7290 0.0 29.749475 2 ATCAACG 7305 0.0 29.623425 3 TCAACGC 7340 0.0 29.546822 4 CAACGCA 7505 0.0 28.960457 5 AACGCAG 7640 0.0 28.440268 6 TCGTATC 35 0.008442957 27.109627 94-95 ACGCAGA 8595 0.0 25.059448 7 CGCAGAG 8715 0.0 24.714397 8 GCAGAGT 8940 0.0 23.93319 9 GTACATG 4165 0.0 23.292196 1 TACATGG 4205 0.0 23.022623 2 GAGTACT 5510 0.0 22.12936 12-13 AGTACTT 5630 0.0 21.910501 12-13 ACATGGG 4235 0.0 21.851027 3 ATACGTC 265 8.021743E-10 21.489483 5 CATGGGA 2360 0.0 20.912788 4 AGAGTAC 8125 0.0 20.700472 10-11 GTACTTT 6130 0.0 20.04595 14-15 >>END_MODULE