FastQCFastQC Report
Thu 26 May 2016
SRR938851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116345
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17121.4714856676264558No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16161.3889724526193648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14051.2076152821350294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.8242726374145859No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.8234131247582621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.3326313979973355No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.2647298981477502No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.23292792986376723No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.20198547423610813No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.12033177188534101No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10486054407151145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.039.8619161
ATGGGGA851.6423906E-533.4611975
GTATCAA13200.031.9613551
CATTATA600.003975914331.602242
CATGGGC1950.031.6022384
CTTCTAG650.005880987729.1712991
GTGTTGC1005.013471E-528.4175479
ATGGGAC1005.013471E-528.4175477
GTACATG9200.028.3389641
ATGGGCA856.448636E-427.8843295
TAGAATA856.448636E-427.8843294
CATGGGG2053.092282E-1127.7483064
TACATGG9350.027.377342
ACATGGG9350.026.8703523
GGGGATT458.8749116E-426.41479774-75
AACGCAG16450.026.2005796
TATCAAC16150.026.1232132
ATCAACG16150.026.1232133
GGGCTTG1109.5920695E-525.8341337
TGGGGAG1109.5920695E-525.8341336
Produced by FastQC (version 0.11.5)