##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938851_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116345 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.685358201899522 31.0 31.0 34.0 27.0 34.0 2 30.704198719326143 33.0 31.0 34.0 27.0 34.0 3 30.643860930852206 33.0 31.0 34.0 26.0 34.0 4 34.18334264472044 37.0 35.0 37.0 30.0 37.0 5 34.193209850015045 37.0 35.0 37.0 30.0 37.0 6 34.285091752976065 37.0 35.0 37.0 30.0 37.0 7 34.20188233271735 37.0 35.0 37.0 30.0 37.0 8 34.17011474493962 37.0 35.0 37.0 30.0 37.0 9 35.63205982208088 39.0 37.0 39.0 30.0 39.0 10-11 35.520980703940864 39.0 35.0 39.0 30.0 39.0 12-13 35.39509648029568 39.0 35.0 39.0 30.0 39.0 14-15 36.36080192530835 40.0 36.0 41.0 29.0 41.0 16-17 36.24717005457906 39.5 36.0 41.0 28.5 41.0 18-19 36.25615196183764 40.0 36.0 41.0 29.0 41.0 20-21 36.1880527740771 40.0 36.0 41.0 29.0 41.0 22-23 36.00424169495896 39.0 35.5 41.0 27.5 41.0 24-25 35.88124113627573 39.0 35.0 41.0 27.0 41.0 26-27 35.69318836219863 39.0 35.0 41.0 27.0 41.0 28-29 35.483183634879026 39.0 35.0 41.0 26.5 41.0 30-31 35.08145171687653 38.5 35.0 41.0 25.5 41.0 32-33 34.65887661695818 38.0 35.0 40.0 24.5 41.0 34-35 34.1987837895913 38.0 34.5 40.0 22.0 41.0 36-37 33.81489965189738 38.0 34.0 40.0 17.5 41.0 38-39 33.52373544200438 38.0 34.0 40.0 15.5 41.0 40-41 33.178512183591906 38.0 33.0 40.0 9.5 41.0 42-43 32.72046069878379 37.5 33.0 40.0 4.0 41.0 44-45 32.28394000601659 38.0 33.0 40.0 2.0 41.0 46-47 31.735235721345994 37.0 31.5 40.0 2.0 41.0 48-49 31.24615153208131 37.0 31.0 40.0 2.0 41.0 50-51 30.038334264472045 36.0 28.5 39.0 2.0 40.5 52-53 30.081318492414802 35.5 29.0 39.5 2.0 40.0 54-55 30.476853324165198 36.5 30.0 40.0 2.0 41.0 56-57 30.328308049336023 36.0 29.0 40.0 2.0 41.0 58-59 30.082208088014095 36.0 28.5 40.0 2.0 41.0 60-61 29.778005071124674 35.5 28.0 40.0 2.0 41.0 62-63 29.40674287678886 35.0 27.0 39.5 2.0 41.0 64-65 29.011066225450172 35.0 26.0 39.0 2.0 41.0 66-67 28.59657484206455 35.0 26.0 39.0 2.0 41.0 68-69 28.146460956637586 35.0 24.5 38.0 2.0 40.0 70-71 27.698577506553782 35.0 22.5 37.5 2.0 40.0 72-73 27.23981262624092 35.0 21.0 37.0 2.0 39.5 74-75 26.741488675920753 34.0 19.0 36.5 2.0 39.0 76-77 26.267286948300313 34.0 14.5 36.0 2.0 39.0 78-79 25.773630151703983 34.0 6.0 35.5 2.0 37.5 80-81 25.276973655937084 33.5 2.0 35.0 2.0 37.0 82-83 24.760423739739565 33.0 2.0 35.0 2.0 37.0 84-85 24.288340710816968 33.0 2.0 35.0 2.0 36.0 86-87 23.83739309811337 33.0 2.0 35.0 2.0 36.0 88-89 23.373294941768016 32.5 2.0 35.0 2.0 35.5 90-91 22.884838196742447 32.0 2.0 35.0 2.0 35.0 92-93 22.3504319051098 31.5 2.0 35.0 2.0 35.0 94-95 21.765963298809574 31.0 2.0 35.0 2.0 35.0 96-97 21.16292492156947 31.0 2.0 35.0 2.0 35.0 98-99 20.517774721732778 30.0 2.0 34.5 2.0 35.0 100-101 18.79843568696549 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6270334794317733 1101 2 0.342519758895822 1101 3 0.5828820365603491 1101 4 0.36635036530653764 1101 5 -0.0856380435306221 1101 6 0.015949562908680548 1101 7 -0.13974329266972774 1101 8 -0.2189768770104905 1101 9 -0.06570215602862106 1101 10-11 0.5903411916022065 1101 12-13 0.7510724826317059 1101 14-15 0.36451865263656913 1101 16-17 0.7155615799761961 1101 18-19 0.3743236314076128 1101 20-21 0.11654643719501934 1101 22-23 0.7523260513181853 1101 24-25 0.6065410476878768 1101 26-27 0.5847300396462245 1101 28-29 0.2603592851887342 1101 30-31 0.2506723226763725 1101 32-33 0.9499443625867556 1101 34-35 0.957017257120917 1101 36-37 0.7667977241378523 1101 38-39 0.054632054169935884 1101 40-41 0.030763853200298286 1101 42-43 0.4290505765303081 1101 44-45 0.7248881403337961 1101 46-47 1.0867712420265683 1101 48-49 1.5523017854385337 1101 50-51 1.7783382658873919 1101 52-53 1.2870704432754962 1101 54-55 1.5460658059599943 1101 56-57 1.6028375115525435 1101 58-59 1.1356130407711653 1101 60-61 0.7436459939924376 1101 62-63 0.521246597079088 1101 64-65 0.15491347835970615 1101 66-67 0.5665157857117471 1101 68-69 0.9251212122460544 1101 70-71 0.7462152721367978 1101 72-73 1.0861604153739073 1101 74-75 0.6997587689839087 1101 76-77 1.2030073885193602 1101 78-79 1.1641466710462467 1101 80-81 0.4029007471013344 1101 82-83 0.19157414360604363 1101 84-85 0.26447996826767195 1101 86-87 0.4382207998587546 1101 88-89 0.7799044265550528 1101 90-91 0.629615565113216 1101 92-93 0.34185928879128014 1101 94-95 0.18876589425090984 1101 96-97 0.590134696422357 1101 98-99 0.7677176944561062 1101 100-101 1.8382836580193995 1102 1 0.911056621398842 1102 2 0.5657507335286667 1102 3 1.036731613774414 1102 4 0.7115217048703997 1102 5 0.9219275995490435 1102 6 0.56254502352283 1102 7 0.8680003655412136 1102 8 0.7910809779071784 1102 9 1.1101767936821076 1102 10-11 1.0322192512372794 1102 12-13 1.2297106668773665 1102 14-15 0.8881056575319803 1102 16-17 1.2385700356949272 1102 18-19 0.9737450822309341 1102 20-21 0.7693724274041855 1102 22-23 0.49994509893723205 1102 24-25 0.5546051331351478 1102 26-27 0.5609650196195233 1102 28-29 0.8676134017886525 1102 30-31 0.8468895011365376 1102 32-33 0.5929350167923602 1102 34-35 1.3276447604586963 1102 36-37 1.0691119208445699 1102 38-39 0.8626872388606301 1102 40-41 1.3551964299693253 1102 42-43 1.3725307723469484 1102 44-45 1.8470065203961 1102 46-47 1.139953262498313 1102 48-49 0.20432670755691262 1102 50-51 0.36405255160167727 1102 52-53 0.7276578932087361 1102 54-55 0.7964440881139758 1102 56-57 0.9028820153353649 1102 58-59 0.7753104150479757 1102 60-61 1.032297529372947 1102 62-63 0.359386338957151 1102 64-65 0.6284337275808944 1102 66-67 0.26882998241846323 1102 68-69 0.42854800352331424 1102 70-71 0.12957085736154283 1102 72-73 0.4301301528015884 1102 74-75 0.5598388757929875 1102 76-77 0.3573910111272802 1102 78-79 -0.07786489993728907 1102 80-81 0.34290964785790123 1102 82-83 0.3339862486349716 1102 84-85 0.17120003947372453 1102 86-87 0.5132096739130496 1102 88-89 0.49218747061379986 1102 90-91 0.21506282813056998 1102 92-93 0.0033635166506478242 1102 94-95 0.2697182752032923 1102 96-97 0.5441622887677049 1102 98-99 -0.33998145997201945 1102 100-101 -0.02437766819332765 1103 1 0.9297482101838881 1103 2 0.8180871821267957 1103 3 0.7657035763912354 1103 4 0.9451665646834897 1103 5 0.4266004967453014 1103 6 0.7774982945508668 1103 7 0.6437013001206537 1103 8 0.706968828374464 1103 9 1.3905506254578057 1103 10-11 0.929415512919519 1103 12-13 0.8932620687465231 1103 14-15 0.9675449098684297 1103 16-17 0.8740840543865218 1103 18-19 0.9083245214832729 1103 20-21 1.16189579189016 1103 22-23 1.6448049120213426 1103 24-25 1.4331098060323413 1103 26-27 1.1450771691522306 1103 28-29 1.3208844298260303 1103 30-31 1.206702585248685 1103 32-33 1.2377948298764778 1103 34-35 1.7809157884960882 1103 36-37 1.597149304022146 1103 38-39 1.049603126711105 1103 40-41 1.0841683925861503 1103 42-43 0.6575775013189116 1103 44-45 0.8096233428260078 1103 46-47 0.9670560662366263 1103 48-49 0.9847005393326107 1103 50-51 0.5229310562745759 1103 52-53 0.70896630442369 1103 54-55 1.100182405870985 1103 56-57 1.3608259405690077 1103 58-59 1.3080206954218063 1103 60-61 0.35472743591500233 1103 62-63 -0.04715571711761868 1103 64-65 -0.08184664625088445 1103 66-67 0.6473346553156638 1103 68-69 -0.23500339834584594 1103 70-71 -0.27697119871322684 1103 72-73 0.16844790981093283 1103 74-75 -0.019600376543461806 1103 76-77 -0.40896412905963686 1103 78-79 -0.12459387189990423 1103 80-81 -0.848679137188828 1103 82-83 -0.36227543360801917 1103 84-85 0.2506392918101703 1103 86-87 -0.4166968681430241 1103 88-89 -0.7975321555544248 1103 90-91 -0.635404461589058 1103 92-93 -0.72560844596617 1103 94-95 -0.7069172388154001 1103 96-97 -1.170790982260332 1103 98-99 -0.8867104319346346 1103 100-101 -0.19727486445501086 1104 1 0.9562001460984426 1104 2 0.794305473181538 1104 3 0.5602629889413038 1104 4 0.8330170319732062 1104 5 1.0423381469455677 1104 6 0.6963067057658208 1104 7 0.4798995644731292 1104 8 0.6584040753704627 1104 9 0.5938216534951906 1104 10-11 0.1504602392212604 1104 12-13 0.2064296254888447 1104 14-15 0.5014234145413283 1104 16-17 0.5759782466428618 1104 18-19 -0.07895017811619454 1104 20-21 0.14095119909978138 1104 22-23 0.07137367036580144 1104 24-25 0.35029104768788244 1104 26-27 0.7284800396462217 1104 28-29 0.8243473804268291 1104 30-31 0.28668422743827904 1104 32-33 0.5419086483010389 1104 34-35 0.5980886856923462 1104 36-37 0.650428676518807 1104 38-39 0.8346915779794557 1104 40-41 0.5218352817717289 1104 42-43 0.7534553384350673 1104 44-45 0.5573286165242735 1104 46-47 0.9900450515503785 1104 48-49 1.5740279759147278 1104 50-51 1.056016837315962 1104 52-53 1.2468918718469233 1104 54-55 1.432375329769517 1104 56-57 1.2087898925049245 1104 58-59 0.6585297074378325 1104 60-61 0.8427531368495806 1104 62-63 0.7724370732695647 1104 64-65 1.0998539545501842 1104 66-67 0.8769324523784157 1104 68-69 1.0905974027222456 1104 70-71 1.228953367374892 1104 72-73 1.0688985106120015 1104 74-75 0.9729730546981905 1104 76-77 1.011638340900312 1104 78-79 0.9665276234271971 1104 80-81 0.992484080434668 1104 82-83 0.7258003340822334 1104 84-85 0.9079323492200544 1104 86-87 0.3742327046206597 1104 88-89 0.0031187122693410174 1104 90-91 -0.5444915777439299 1104 92-93 -0.16052166358966957 1104 94-95 0.37686113234614993 1104 96-97 0.2511466011842636 1104 98-99 0.6174200754084893 1104 100-101 1.1680455627813053 1105 1 0.26821752061581705 1105 2 0.20429582067188434 1105 3 0.7782488319271437 1105 4 1.1213053202614915 1105 5 0.8461508882583075 1105 6 0.8849328318919447 1105 7 0.9907586378322009 1105 8 0.48578502775141175 1105 9 1.0285063381798736 1105 10-11 1.1519885532495664 1105 12-13 1.0406477722069951 1105 14-15 1.1939909821088932 1105 16-17 0.5298472942619057 1105 18-19 1.130308187392174 1105 20-21 0.9774215980701726 1105 22-23 1.4233685223606543 1105 24-25 0.8617534029002414 1105 26-27 -0.6191309642147829 1105 28-29 0.5480040728335283 1105 30-31 0.0379310099350576 1105 32-33 -0.10501059236820254 1105 34-35 0.03333643344009829 1105 36-37 0.07349013083025824 1105 38-39 -0.18063178109389355 1105 40-41 -0.5625437401072872 1105 42-43 -1.0362003887206583 1105 44-45 -1.0931298776842233 1105 46-47 -0.4756483203929953 1105 48-49 -0.5756261424893943 1105 50-51 -0.5143258267767017 1105 52-53 -0.6955550393499905 1105 54-55 -0.7028402429460563 1105 56-57 -1.1916283829133505 1105 58-59 -1.041478336320214 1105 60-61 -1.0026860523396088 1105 62-63 -0.9783673025348101 1105 64-65 -1.0613027378565079 1105 66-67 -1.3348999157039536 1105 68-69 -1.8847886976638542 1105 70-71 -1.4019185759970512 1105 72-73 -0.91010728089379 1105 74-75 -1.3400095707844315 1105 76-77 -1.2604417749298058 1105 78-79 -1.873562466662893 1105 80-81 -1.585902341701754 1105 82-83 -1.6938827418508389 1105 84-85 -1.320590816803115 1105 86-87 -1.7097843481463926 1105 88-89 -2.1557455090948814 1105 90-91 -1.8034603679627175 1105 92-93 -1.1280248810928875 1105 94-95 -1.2888402833552668 1105 96-97 -1.191461185173523 1105 98-99 -0.7419348151964016 1105 100-101 -0.7465297267939874 1106 1 -0.36704881367964504 1106 2 -0.20005999145091735 1106 3 -0.2575248057882398 1106 4 -0.7980509291918452 1106 5 -0.43330332323473897 1106 6 -0.20235834277787035 1106 7 -0.5458459278985828 1106 8 -0.345930182604576 1106 9 -0.8018094144659713 1106 10-11 -0.5580175693779097 1106 12-13 -0.2801341747885431 1106 14-15 0.281763220366571 1106 16-17 -0.05591322353744488 1106 18-19 0.3195848426882435 1106 20-21 -0.11503007690577505 1106 22-23 -0.47092868747053274 1106 24-25 -0.12105950709714364 1106 26-27 -0.0993198910056492 1106 28-29 0.6295224844490264 1106 30-31 0.2963496227226017 1106 32-33 0.49072106161587925 1106 34-35 0.8826760643331255 1106 36-37 -0.20259663554777063 1106 38-39 0.17636113415144905 1106 40-41 0.6063533122849094 1106 42-43 1.4537847420411651 1106 44-45 0.3698719591040245 1106 46-47 0.9295821527986483 1106 48-49 0.7730137595485722 1106 50-51 1.51939235742692 1106 52-53 1.1837417331506686 1106 54-55 1.455867007994204 1106 56-57 1.5629854542247585 1106 58-59 1.4419468364253483 1106 60-61 1.3224254669466688 1106 62-63 1.218518904060133 1106 64-65 1.4736837048969242 1106 66-67 0.8951796784533066 1106 68-69 1.1448623125696784 1106 70-71 1.085836169039247 1106 72-73 0.9035899114441825 1106 74-75 0.6266658424929226 1106 76-77 0.4358321689169564 1106 78-79 1.3184693710000133 1106 80-81 1.164250723985294 1106 82-83 1.101883898575995 1106 84-85 0.8565280496361432 1106 86-87 0.9865333287538078 1106 88-89 0.8749113613678148 1106 90-91 1.4243451074155722 1106 92-93 1.5575781977140757 1106 94-95 1.4687474014168842 1106 96-97 0.96313515874321 1106 98-99 0.4718323500270749 1106 100-101 0.08396095806563153 1107 1 -0.4584301797160961 1107 2 0.08362251578805413 1107 3 -0.302832249153937 1107 4 0.21021001442934306 1107 5 0.24017649281022813 1107 6 -0.18856171528680932 1107 7 -0.1903698590857701 1107 8 -0.1370219897924443 1107 9 0.08943568858290973 1107 10-11 -0.45777910914966924 1107 12-13 -0.46196009882193323 1107 14-15 -1.0446292170376168 1107 16-17 -0.49208253029949134 1107 18-19 -0.7671550653342436 1107 20-21 -1.2072630866145104 1107 22-23 -0.5921601642206582 1107 24-25 -0.7246619598309181 1107 26-27 -0.7086283563437519 1107 28-29 0.14319136538923516 1107 30-31 -0.7596033665466919 1107 32-33 -0.8386521286413142 1107 34-35 -0.7933586827287087 1107 36-37 -1.375182225736836 1107 38-39 -1.0258190736496147 1107 40-41 -1.6910406580778954 1107 42-43 -1.2701975437704434 1107 44-45 -1.504936421069715 1107 46-47 -1.0986924171213026 1107 48-49 -0.9459438285965547 1107 50-51 -0.2894562203281481 1107 52-53 -0.08886940634856444 1107 54-55 -0.38325750231068767 1107 56-57 -0.49390434308404707 1107 58-59 0.15328221370349482 1107 60-61 -0.2389354596416453 1107 62-63 -0.5768737036727885 1107 64-65 -0.902104065499941 1107 66-67 -1.315815041355922 1107 68-69 -1.4266331737188587 1107 70-71 -1.3933837253569372 1107 72-73 -1.621107755052158 1107 74-75 -1.3166572711163447 1107 76-77 -1.0330828370445495 1107 78-79 -0.8141741309587687 1107 80-81 -0.8110090273347552 1107 82-83 -0.5402554303287914 1107 84-85 -0.6207330643137787 1107 86-87 -0.5016288242013935 1107 88-89 -1.104180786477528 1107 90-91 -1.0708856880196187 1107 92-93 -0.8466118891535785 1107 94-95 -0.5592290932177626 1107 96-97 -0.3606171832768901 1107 98-99 -0.4547133832381256 1107 100-101 -0.2307639610282166 1108 1 -0.6322738798755836 1108 2 -1.0146230982470357 1108 3 -0.8270746733963605 1108 4 -1.2033466043904326 1108 5 -0.8172397751323501 1108 6 -0.7139205669614554 1108 7 -0.6072757602021994 1108 8 -0.6354270934607058 1108 9 -0.9834510737775375 1108 10-11 -1.1691826179215994 1108 12-13 -1.7781482965890731 1108 14-15 -1.2463038581859465 1108 16-17 -1.035304220889607 1108 18-19 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2316 90-91 -1.81434047884283 2316 92-93 -1.5561260591940673 2316 94-95 -1.3225894171043997 2316 96-97 -1.1193891131014517 2316 98-99 -1.4634178366794224 2316 100-101 -1.3256357559000165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3068.0 3 566.0 4 468.0 5 401.0 6 407.0 7 421.0 8 494.0 9 515.0 10 631.0 11 798.0 12 1111.0 13 1468.0 14 1481.0 15 1655.0 16 2083.0 17 2625.0 18 2115.0 19 1356.0 20 1244.0 21 1262.0 22 1312.0 23 1387.0 24 1481.0 25 1691.0 26 1828.0 27 2158.0 28 2418.0 29 2700.0 30 3072.0 31 3610.0 32 4254.0 33 5051.0 34 6695.0 35 9642.0 36 11101.0 37 15559.0 38 15465.0 39 2751.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.475528929745735 21.885382774707775 15.59245296011293 19.04663533543356 2 15.941954186128529 23.565843462104205 39.35984447713177 21.132357874635492 3 18.296060553930847 29.751419232754174 32.298296920694995 19.654223292619992 4 12.310193828127018 20.09170918038146 38.18835654739885 29.409740444092673 5 11.405584477046853 39.185991481306196 34.03605386567999 15.372370175966957 6 25.541464253471474 38.714586647177676 19.226172563518336 16.51777653583251 7 22.943842059554417 37.8986560272406 22.859575053526747 16.29792685967824 8 24.300503877968666 34.0057438648988 21.889456396498648 19.80429586063389 9 26.43386589161952 17.70856622009734 23.166285448948354 32.69128243933479 10-11 22.733838931022028 28.897745447041224 27.75198197733409 20.616433644602658 12-13 24.873703173436176 28.421622876601017 28.39754586455821 18.3071280854046 14-15 22.611989389008077 28.362046356448502 29.1742085825211 19.85175567202232 16-17 18.250334274892193 31.5809160206885 29.865043230019822 20.303706474399487 18-19 17.845902485166395 28.788373892854075 35.125978158053144 18.23974546392639 20-21 19.59798778914782 27.877719494367533 34.942385415770914 17.581907300713734 22-23 19.37414802169024 28.16677918872314 34.514743256201974 17.944329533384646 24-25 18.475016558142734 28.883852155139046 34.97208770224589 17.669043584472334 26-27 18.228893380929854 28.602640746634556 35.17655154617006 17.991914326265537 28-29 18.095221712373835 28.391913336286343 35.66535214659551 17.847512804744316 30-31 18.47844783102274 28.69250748219753 35.06570573463139 17.763338952148334 32-33 17.83553692357304 29.26109857035365 34.96678490809416 17.936579597979147 34-35 18.04912105292645 28.858510853778892 34.71399204444081 18.378376048853855 36-37 18.404865604509922 29.04988625915646 34.03808052074746 18.507167615586166 38-39 18.21947956673715 28.90562292322962 34.433608078367065 18.441289431666164 40-41 18.01586856445148 28.845149905729166 34.68907882077324 18.449902709046118 42-43 18.043052330850433 28.995157907456477 34.0646820704477 18.89710769124539 44-45 18.059320131899245 29.174939143345473 33.773285223486354 18.992455501268925 46-47 18.303336354611766 29.092321636669688 33.04458543743796 19.55975657128059 48-49 18.32611708028819 29.365041678717734 32.533573923065966 19.775267317928108 50-51 18.31256447554138 29.58256538197577 32.114970411391724 19.989899731091132 52-53 18.044200802866143 29.729356412137953 32.37449820865887 19.85194457633703 54-55 18.41862191605808 29.556205866611418 31.860206142849744 20.16496607448076 56-57 18.18421120767777 29.903251141394875 31.562481120595166 20.350056530332193 58-59 18.316306279876436 28.76637271989922 32.10519957892557 20.812121421298773 60-61 18.492621844172312 29.02477214201602 31.919528281133402 20.563077732678263 62-63 18.49473502503021 29.054030726739168 31.95063007077507 20.50060417745555 64-65 18.815001575795776 28.681641763337062 31.908785169387254 20.59457149147991 66-67 18.496995026250346 28.78600787510362 31.379956479690524 21.337040618955513 68-69 18.604189245788948 28.76382274075641 31.385231850703605 21.246756162751034 70-71 18.79023379314811 28.69546445610172 31.356777410789455 21.15752433996071 72-73 18.980210330000517 28.3777133088121 31.049146074012672 21.592930287174706 74-75 18.897066758757635 28.579137078845697 30.87392890289439 21.64986725950228 76-77 19.079373300530882 28.336570417368034 31.03198239026285 21.552073891838234 78-79 19.125433119452513 28.330650833444803 30.971438927460316 21.57247711964237 80-81 19.30991926331551 28.367013174190152 30.515963942504783 21.807103619989558 82-83 19.288682885145718 28.333570697981536 30.426263750965642 21.9514826659071 84-85 19.525889227422986 27.72292182739364 30.4408807257097 22.310308219473672 86-87 19.886226304340322 27.41704361037257 30.468129553537516 22.228600531749596 88-89 20.695415695415694 26.803936803936807 30.303461970128637 22.197185530518865 90-91 20.555910487964912 26.670580515937424 30.71450771834099 22.059001277756675 92-93 21.07844195312736 26.509071914836667 30.20000086337519 22.212485268660775 94-95 21.226720542906726 26.08507956242823 30.031686827086624 22.65651306757842 96-97 21.469246078858863 25.635822802844206 29.814056098329655 23.080875019967277 98-99 21.60213768006458 25.556759463507916 29.79283669971898 23.048266156708525 100-101 22.802708873762857 24.850765917223104 29.950837998472053 22.39568721054199 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1408.0 1 879.5 2 365.0 3 386.5 4 318.5 5 301.0 6 450.5 7 719.5 8 970.0 9 998.0 10 785.5 11 551.5 12 484.0 13 497.0 14 482.0 15 452.0 16 437.5 17 449.5 18 498.5 19 500.5 20 513.0 21 545.5 22 594.0 23 685.0 24 743.5 25 881.5 26 1110.0 27 1371.0 28 1553.5 29 1710.0 30 1956.5 31 2204.5 32 2550.5 33 2907.0 34 3352.5 35 3753.0 36 3972.0 37 4180.0 38 4165.5 39 3897.0 40 3592.5 41 3500.0 42 3450.5 43 3396.5 44 3391.5 45 3369.0 46 3228.0 47 2993.0 48 2891.5 49 2852.5 50 2790.0 51 2629.0 52 2414.0 53 2207.0 54 2002.0 55 1886.0 56 1749.0 57 1538.0 58 1428.0 59 1342.5 60 1228.0 61 1125.0 62 1016.5 63 857.5 64 701.5 65 621.5 66 538.0 67 493.0 68 460.5 69 400.0 70 389.5 71 349.0 72 288.5 73 254.5 74 236.0 75 198.0 76 145.0 77 118.0 78 95.0 79 72.5 80 56.0 81 45.5 82 38.0 83 30.0 84 17.0 85 12.5 86 10.0 87 4.5 88 4.0 89 3.5 90 2.5 91 1.5 92 1.0 93 1.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14353861360608533 2 0.07907516438179553 3 0.07305857578752847 4 0.09282736688297735 5 0.11173664532210237 6 0.03438050625295458 7 0.04125660750354549 8 0.04039709484722163 9 0.04383514547251709 10-11 0.04039709484722163 12-13 0.04426490180067902 14-15 0.04340538914435515 16-17 0.04168636383170742 18-19 0.04727319609781254 20-21 0.04727319609781254 22-23 0.0614551549271563 24-25 0.07563711375650006 26-27 0.07735613906914779 28-29 0.06833125617774721 30-31 0.05930637328634664 32-33 0.0494219777386222 34-35 0.27977136963341787 36-37 0.4396407237096566 38-39 0.4125660750354549 40-41 0.3915080149555202 42-43 0.41772315097339807 44-45 0.4288968155056083 46-47 0.42975632816193216 48-49 0.4456573123039237 50-51 0.4353431604280373 52-53 0.43835145472517084 54-55 0.4323348661309038 56-57 0.41084704972280717 58-59 0.38764020800206284 60-61 0.36830117323477585 62-63 0.41686363831707424 64-65 0.45597146417981005 66-67 0.4615582964459152 68-69 0.4714426919936396 70-71 0.4619880527740771 72-73 0.4529631698826765 74-75 0.4447977996475998 76-77 0.42975632816193216 78-79 0.4044007048003782 80-81 0.4078387554256736 82-83 0.42073144527053163 84-85 0.41772315097339807 86-87 0.43061584081825605 88-89 0.44264901800679013 90-91 0.4443680433194379 92-93 0.4473763376165714 94-95 0.4503846319137049 96-97 0.45597146417981005 98-99 0.4456573123039237 100-101 0.4327646224590657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 116345.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.55073273453952 #Duplication Level Percentage of deduplicated Percentage of total 1 87.63784173605193 57.44724741071813 2 6.567888284271947 8.610597791052474 3 2.023208549137874 3.978684086123168 4 0.9834130990624795 2.578537968971593 5 0.5730020323870714 1.8780351540676434 6 0.4340129810529076 1.7069921354591948 7 0.3251819314233266 1.4921139713782285 8 0.239952796171245 1.2583265288581373 9 0.1861928800891628 1.0984571747818985 >10 0.9781682292008129 11.465039322704026 >50 0.0367140890316659 1.5127422751300013 >100 0.007867304792499835 1.2574670162018136 >500 0.0026224349308332784 1.6476857621728478 >1k 0.003933652396249918 4.06807340238085 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 1.4714856676264558 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1616 1.3889724526193648 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1405 1.2076152821350294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 959 0.8242726374145859 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 958 0.8234131247582621 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 387 0.3326313979973355 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 308 0.2647298981477502 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 271 0.23292792986376723 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 235 0.20198547423610813 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.12033177188534101 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 122 0.10486054407151145 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.595126563238644E-4 0.0 18-19 0.0 0.0 0.0 8.595126563238644E-4 0.0 20-21 0.0 0.0 0.0 0.002148781640809661 0.0 22-23 0.0 0.0 0.0 0.009024882891400576 0.0 24-25 0.0 0.0 0.0 0.022347329064420475 0.0 26-27 0.0 0.0 0.0 0.0494219777386222 0.0 28-29 0.0 0.0 0.0 0.09325712321113928 0.0 30-31 0.0 0.0 0.0 0.2110103571275087 0.0 32-33 0.0 0.0 0.0 0.3880699643302248 0.0 34-35 0.0 0.0 0.0 0.6497915681808415 0.0 36-37 0.0 0.0 0.0 1.0494649533714384 0.0 38-39 0.0 0.0 0.0 1.5350896041944218 0.0 40-41 0.0 0.0 0.0 2.046929391035283 0.0 42-43 0.0 0.0 0.0 2.4882891400575877 0.0 44-45 0.0 0.0 0.0 2.9618806136920366 0.0 46-47 0.0 0.0 0.0 3.5081009067858524 0.0 48-49 0.0 0.0 0.0 3.9984528772186168 0.0 50-51 0.0 0.0 0.0 4.525763891873307 0.0 52-53 0.0 0.0 0.0 5.03115733379174 0.0 54-55 0.0 0.0 0.0 5.601443981262625 0.0 56-57 0.0 0.0 0.0 6.216855043190511 0.0 58-59 0.0 0.0 0.0 6.84601830761958 0.0 60-61 0.0 0.0 0.0 7.537925995960291 0.0 62-63 0.0 0.0 0.0 8.170957067342815 0.0 64-65 0.0 0.0 0.0 8.849542309510507 0.0 66-67 0.0 0.0 0.0 9.58829343762087 0.0 68-69 0.0 0.0 0.0 10.38463191370493 0.0 70-71 0.0 0.0 0.0 11.271219218702996 0.0 72-73 0.0 0.0 0.0 12.1088143022906 0.0 74-75 0.0 0.0 0.0 12.963169882676523 0.0 76-77 0.0 0.0 0.0 13.896170871116077 0.0 78-79 0.0 0.0 0.0 14.863122609480424 0.0 80-81 0.0 0.0 0.0 15.861446559800592 0.0 82-83 0.0 0.0 0.0 16.842150500666122 0.0 84-85 0.0 0.0 0.0 17.8335983497357 0.0 86-87 0.0 0.0 0.0 18.835790107009323 0.0 88-89 0.0 0.0 0.0 19.848725772487 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 880 0.0 39.861916 1 ATGGGGA 85 1.6423906E-5 33.461197 5 GTATCAA 1320 0.0 31.961355 1 CATTATA 60 0.0039759143 31.60224 2 CATGGGC 195 0.0 31.602238 4 CTTCTAG 65 0.0058809877 29.171299 1 GTGTTGC 100 5.013471E-5 28.417547 9 ATGGGAC 100 5.013471E-5 28.417547 7 GTACATG 920 0.0 28.338964 1 ATGGGCA 85 6.448636E-4 27.884329 5 TAGAATA 85 6.448636E-4 27.884329 4 CATGGGG 205 3.092282E-11 27.748306 4 TACATGG 935 0.0 27.37734 2 ACATGGG 935 0.0 26.870352 3 GGGGATT 45 8.8749116E-4 26.414797 74-75 AACGCAG 1645 0.0 26.200579 6 TATCAAC 1615 0.0 26.123213 2 ATCAACG 1615 0.0 26.123213 3 GGGCTTG 110 9.5920695E-5 25.834133 7 TGGGGAG 110 9.5920695E-5 25.834133 6 >>END_MODULE