FastQCFastQC Report
Thu 26 May 2016
SRR938851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116345
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT20641.7740341226524559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15751.3537324337100864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15621.3425587691778762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14231.223086509948859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.8646697322618077No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT9810.8431819158537109RNA PCR Primer, Index 26 (100% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.7297262452189608No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT5700.48992221410460274RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG4030.34638360049851735RNA PCR Primer, Index 26 (100% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.3137221195582105No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.25441574627186386No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.22003524001890928No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.17620009454639218No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1890.16244789204521035RNA PCR Primer, Index 26 (100% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1620.13924105032446604RNA PCR Primer, Index 26 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.1349434870428467No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG1340.11517469594739783RNA PCR Primer, Index 26 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT1190.10228200610253985RNA PCR Primer, Index 26 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAA200.002075369771.198088
TGGACTG352.7875265E-454.2461625
GTCCAGA502.7734603E-547.608671
TAGTCAA459.6238346E-442.191469
GGTATCA9300.041.977531
ATTATGT500.001615624237.9723138
GTATCAA15050.035.4297071
TACTATG550.002579345134.5202877
CACCGCC300.003859612931.80769384-85
GGATAGA300.003958631331.64359532-33
TATCAAC17800.029.5992032
ATCAACG17950.029.3518583
TCAACGC18150.029.0284214
CAACGCA18450.028.5564165
AACGCAG18200.028.1662756
AGGTAGC700.00839091427.1230817
CCCTAGG700.00839091427.1230818
ATTCGTG350.008407894527.12308154-55
CGCAGAG20000.025.6313118
GAGTACT11800.025.34169412-13
Produced by FastQC (version 0.11.5)