##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938851_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116345 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79325282564786 31.0 31.0 34.0 30.0 34.0 2 32.015531393699774 33.0 31.0 34.0 30.0 34.0 3 32.034853238213934 33.0 31.0 34.0 30.0 34.0 4 35.75842537281361 37.0 35.0 37.0 33.0 37.0 5 35.67720142679101 37.0 35.0 37.0 33.0 37.0 6 35.727319609781254 37.0 35.0 37.0 33.0 37.0 7 35.642743564398984 37.0 35.0 37.0 33.0 37.0 8 35.636744166057845 37.0 35.0 37.0 33.0 37.0 9 37.20804503846319 39.0 37.0 39.0 33.0 39.0 10-11 37.149490738751126 39.0 37.0 39.0 33.0 39.0 12-13 37.066307103872106 39.0 37.0 39.0 33.0 39.0 14-15 38.15204349134041 40.0 37.0 41.0 33.0 41.0 16-17 38.06574842064549 40.0 37.0 41.0 33.0 41.0 18-19 38.122798573208996 40.0 37.0 41.0 33.0 41.0 20-21 38.179311530362284 40.0 37.0 41.0 33.0 41.0 22-23 38.060445227555974 40.0 37.0 41.0 33.0 41.0 24-25 37.914671881043446 40.0 37.0 41.0 33.0 41.0 26-27 37.641282392883234 40.0 36.0 41.0 32.5 41.0 28-29 37.38513902617216 39.5 36.0 41.0 32.0 41.0 30-31 36.97825432979501 39.0 35.0 41.0 31.0 41.0 32-33 36.47435643989858 38.5 35.0 41.0 30.5 41.0 34-35 36.1795178133998 38.0 35.0 41.0 30.0 41.0 36-37 35.91531651553569 38.0 35.0 41.0 30.0 41.0 38-39 35.568077699944126 38.0 35.0 41.0 28.5 41.0 40-41 35.27417594224075 38.0 35.0 41.0 27.0 41.0 42-43 34.93386479865916 38.0 35.0 40.5 25.5 41.0 44-45 34.44965404615583 38.0 34.0 40.0 23.5 41.0 46-47 33.794868709441744 38.0 33.0 40.0 18.5 41.0 48-49 33.24270918389273 38.0 33.0 40.0 10.0 41.0 50-51 32.82670505823198 38.0 33.0 40.0 2.0 41.0 52-53 32.601568610597795 37.0 33.0 40.0 2.0 41.0 54-55 32.25387855086166 37.0 32.5 40.0 2.0 41.0 56-57 31.830525591989343 36.5 31.5 40.0 2.0 41.0 58-59 31.360075637113756 36.0 31.0 40.0 2.0 41.0 60-61 30.777326056126178 35.5 30.5 39.5 2.0 41.0 62-63 30.21899522970476 35.0 29.5 39.0 2.0 41.0 64-65 29.68539687996906 35.0 28.0 39.0 2.0 40.0 66-67 29.051837208302892 35.0 27.5 38.5 2.0 40.0 68-69 28.45782371395419 35.0 26.0 38.0 2.0 40.0 70-71 27.96714512871202 34.5 25.5 37.0 2.0 39.5 72-73 27.655429971206324 34.0 25.0 37.0 2.0 39.0 74-75 27.251080837165325 34.0 24.0 36.0 2.0 39.0 76-77 25.364828742103228 31.5 20.0 34.5 2.0 36.5 78-79 26.284911255318235 33.5 21.0 35.0 2.0 37.0 80-81 26.286363831707423 34.0 20.5 35.0 2.0 37.0 82-83 25.96376724397267 34.0 19.5 35.0 2.0 36.5 84-85 25.59721517899351 33.0 17.0 35.0 2.0 36.0 86-87 25.06538742532984 33.0 8.5 35.0 2.0 36.0 88-89 24.545661609867206 33.0 2.0 35.0 2.0 35.0 90-91 24.120477889036916 32.0 2.0 35.0 2.0 35.0 92-93 23.829631698826766 32.0 2.0 35.0 2.0 35.0 94-95 23.448330396665092 32.0 2.0 35.0 2.0 35.0 96-97 22.96151961837638 32.0 2.0 34.5 2.0 35.0 98-99 22.3015428252181 31.0 2.0 34.0 2.0 35.0 100-101 19.81924019081181 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13516896797677447 1101 2 0.1777068244714215 1101 3 0.07982818212045828 1101 4 0.22496111402278274 1101 5 0.4490112372397306 1101 6 0.2624688599090774 1101 7 0.0058348541085635475 1101 8 0.1446328849264873 1101 9 0.3249472534274176 1101 10-11 0.3458372516098649 1101 12-13 0.2835355650625999 1101 14-15 0.26531014055667157 1101 16-17 0.017198790107258333 1101 18-19 0.18260305242141328 1101 20-21 0.48274865786579824 1101 22-23 0.2009844672100769 1101 24-25 0.2504462673875807 1101 26-27 0.30776250404569083 1101 28-29 0.4583285106498778 1101 30-31 -0.019975094325353382 1101 32-33 -0.3240883641955108 1101 34-35 -0.13902925164560997 1101 36-37 -0.751237554540424 1101 38-39 -0.41872092177730735 1101 40-41 0.4505810132966559 1101 42-43 -0.3158598281288718 1101 44-45 -0.5470853729375094 1101 46-47 -0.5324971239774712 1101 48-49 -0.1028710741836818 1101 50-51 0.11393059815909368 1101 52-53 -0.6343964207237391 1101 54-55 -1.128463769716621 1101 56-57 -1.8464793301339313 1101 58-59 -1.348351968184069 1101 60-61 -0.7217971346464118 1101 62-63 -0.952439567713256 1101 64-65 -0.6856989626040324 1101 66-67 -0.8193189202325506 1101 68-69 -0.9162870662817397 1101 70-71 -0.40236452845539006 1101 72-73 -0.017775296926217266 1101 74-75 -0.062207635150812735 1101 76-77 -0.30022921560609817 1101 78-79 -0.3937345420800291 1101 80-81 -0.331136204456417 1101 82-83 0.06927274347549428 1101 84-85 -0.14622227015899725 1101 86-87 0.44972209379524486 1101 88-89 0.3434191155692581 1101 90-91 -0.02947713585242795 1101 92-93 -0.012084598510739397 1101 94-95 0.16800370994426217 1101 96-97 -0.19713369135942216 1101 98-99 0.06332954418908088 1101 100-101 0.6580218887839102 1102 1 0.024087526054209718 1102 2 0.3608843945648772 1102 3 0.4887289386847655 1102 4 0.40270922261201747 1102 5 0.20406241658997004 1102 6 0.2538351260417002 1102 7 0.3490480272282781 1102 8 0.616372982834811 1102 9 0.520941912973484 1102 10-11 0.2809507362560595 1102 12-13 0.5951510523790233 1102 14-15 0.6127066692616197 1102 16-17 0.5998868185896811 1102 18-19 0.5465104845531457 1102 20-21 0.3636120312525293 1102 22-23 0.33258215301336946 1102 24-25 0.4013585949354237 1102 26-27 0.6131919655499232 1102 28-29 0.3824495609391505 1102 30-31 0.7842972688255117 1102 32-33 0.3116481734101839 1102 34-35 0.747575109725112 1102 36-37 0.6016774432343865 1102 38-39 0.6637000840081768 1102 40-41 0.8674034432032016 1102 42-43 0.7056354989739404 1102 44-45 0.9665327846058815 1102 46-47 0.6652331830986355 1102 48-49 0.5636620811345168 1102 50-51 0.6768144432859273 1102 52-53 1.054655648702159 1102 54-55 0.7768321804391434 1102 56-57 1.0100849778322498 1102 58-59 0.9232101145929725 1102 60-61 1.7360577830215895 1102 62-63 0.8133370232969774 1102 64-65 1.2387336141649925 1102 66-67 1.414459450928998 1102 68-69 1.601781469543809 1102 70-71 1.410096530734645 1102 72-73 1.1248593270168143 1102 74-75 1.4351221378798975 1102 76-77 1.6567356264054744 1102 78-79 1.2827229568073726 1102 80-81 1.1944534706659695 1102 82-83 1.7236563660834143 1102 84-85 1.6414056782166178 1102 86-87 1.2782490185838498 1102 88-89 1.6683412339493202 1102 90-91 2.0508521921404537 1102 92-93 2.5437676489302916 1102 94-95 2.021363745547287 1102 96-97 2.186711435476358 1102 98-99 2.5344465891379038 1102 100-101 2.911871465997976 1103 1 0.28576976904486173 1103 2 0.15527691792936338 1103 3 0.1990093125165373 1103 4 0.22513912915407275 1103 5 0.10125867827221668 1103 6 0.12299400454637066 1103 7 0.22755270012547157 1103 8 0.31730756227406687 1103 9 0.5396335017585301 1103 10-11 0.31366101662988655 1103 12-13 -0.4562508167798569 1103 14-15 -0.4527138914860487 1103 16-17 -0.09170196645705175 1103 18-19 0.08856655931950286 1103 20-21 0.14865876022449243 1103 22-23 0.3933298165647727 1103 24-25 0.35462962297281564 1103 26-27 0.3421639281667481 1103 28-29 0.12076731794849849 1103 30-31 0.125418764152613 1103 32-33 0.2555734070550457 1103 34-35 -0.14962115195713466 1103 36-37 -0.41234124835440156 1103 38-39 -0.9484494487020996 1103 40-41 -0.7213816035257778 1103 42-43 -0.7149252486896174 1103 44-45 -1.6082335705343027 1103 46-47 -1.4983182187705282 1103 48-49 -2.0391416571832366 1103 50-51 -1.8839332202654724 1103 52-53 -1.356559304568865 1103 54-55 -1.7979341747010444 1103 56-57 -0.7889804427285014 1103 58-59 -1.17492072652853 1103 60-61 -1.5302973571653276 1103 62-63 -1.2427377430581608 1103 64-65 -0.51360283443314 1103 66-67 -0.3659143808467036 1103 68-69 -0.6832652594281541 1103 70-71 -0.024482908517693858 1103 72-73 0.10149484103551032 1103 74-75 0.4257763434873709 1103 76-77 -0.460086803501067 1103 78-79 0.31543323718120675 1103 80-81 0.22716375103980013 1103 82-83 0.17225449692453765 1103 84-85 -0.4894354432787118 1103 86-87 -0.4740874300142792 1103 88-89 0.50478983208016 1103 90-91 -0.5846618265511374 1103 92-93 -0.3534286127519515 1103 94-95 -0.7262998058545804 1103 96-97 -0.23384931218719274 1103 98-99 -0.31134780338546264 1103 100-101 0.7529929613250772 1104 1 0.022668967976773757 1104 2 0.46877825304284926 1104 3 0.25959008688236196 1104 4 0.07377063783231108 1104 5 0.34424933247782263 1104 6 0.22020695514716948 1104 7 0.25166818744189356 1104 8 0.4809424087360128 1104 9 0.43566153914170513 1104 10-11 0.3297658230384357 1104 12-13 0.21835699363403194 1104 14-15 0.41352442627096053 1104 16-17 0.5392225996310742 1104 18-19 0.4641506714690351 1104 20-21 0.4750105626277019 1104 22-23 0.3887820862576987 1104 24-25 0.41354150548281865 1104 26-27 1.1303815516647475 1104 28-29 0.8407689868403523 1104 30-31 0.9952034771032103 1104 32-33 1.3931735405663943 1104 34-35 0.15144693883058835 1104 36-37 0.31275054069766384 1104 38-39 0.6604457448893584 1104 40-41 0.5987952990109449 1104 42-43 0.9323544575854186 1104 44-45 0.4761289127767796 1104 46-47 -0.0655328382631879 1104 48-49 0.17718844962584512 1104 50-51 0.24607345530194635 1104 52-53 -0.033503563580886464 1104 54-55 0.7557624207595737 1104 56-57 0.6091754317708293 1104 58-59 0.9614694603873595 1104 60-61 1.282369532020251 1104 62-63 0.6237509084772199 1104 64-65 0.6062653231102537 1104 66-67 0.540502508338875 1104 68-69 0.42984388609921353 1104 70-71 0.7964449953541362 1104 72-73 0.36764136974044703 1104 74-75 0.6208280791349026 1104 76-77 0.24113983201295142 1104 78-79 0.6315630769675913 1104 80-81 0.2923757003054881 1104 82-83 0.5296894101421614 1104 84-85 0.9293729679362421 1104 86-87 1.4053768557000055 1104 88-89 1.3963953060454486 1104 90-91 1.3708204831951925 1104 92-93 1.1590463538702132 1104 94-95 0.9924084718490249 1104 96-97 1.3073305943548625 1104 98-99 1.3529128775224173 1104 100-101 1.7702242697362927 1105 1 -0.059940427132620044 1105 2 -0.09436524760065268 1105 3 0.00106370335597461 1105 4 0.14439483345650217 1105 5 0.27217726040574775 1105 6 0.17443797187819143 1105 7 0.19656844484214986 1105 8 0.2295749698685725 1105 9 0.39624712472728874 1105 10-11 0.38792219369480563 1105 12-13 0.13964882117585375 1105 14-15 -3.269250803938917E-4 1105 16-17 0.19776507067354032 1105 18-19 0.5638128336311965 1105 20-21 0.2688490439661848 1105 22-23 0.05001921624482719 1105 24-25 -0.10592438898306966 1105 26-27 -0.47563517935198973 1105 28-29 0.20736325968462666 1105 30-31 0.1482359738857113 1105 32-33 0.014392974285819093 1105 34-35 -0.057047269663627276 1105 36-37 -0.22614102944389458 1105 38-39 -0.21640431946070748 1105 40-41 -0.48815772544208613 1105 42-43 -0.5149589272279655 1105 44-45 -0.6203157461678828 1105 46-47 -1.0057274972078432 1105 48-49 -1.197851769164373 1105 50-51 -0.6952071109786147 1105 52-53 -1.0156062019335224 1105 54-55 -0.3347700760229273 1105 56-57 -0.2207393043939092 1105 58-59 -0.49996071979282064 1105 60-61 -0.7896221024713803 1105 62-63 -0.6883469036205945 1105 64-65 -0.25558313248820497 1105 66-67 -0.7378517387653716 1105 68-69 -0.55772850772318 1105 70-71 -0.14740957350043615 1105 72-73 -0.44312922228014173 1105 74-75 -0.47983955278272816 1105 76-77 -0.7895471049239866 1105 78-79 -1.2392943876398768 1105 80-81 -0.9823588556790668 1105 82-83 -0.20048272627997576 1105 84-85 0.07295404901732283 1105 86-87 -0.09570905163590027 1105 88-89 0.22900470115484595 1105 90-91 -0.7476238539991442 1105 92-93 -0.5217371081632507 1105 94-95 -0.05786501592446314 1105 96-97 -0.3258338200595503 1105 98-99 0.06989325075278785 1105 100-101 0.3065418373038611 1106 1 0.3282341552167196 1106 2 0.16217284388889652 1106 3 -0.529418234892951 1106 4 -0.1538645910165144 1106 5 0.2471619538370433 1106 6 -0.01982425151025069 1106 7 -0.1953671801031831 1106 8 -0.005621391541382081 1106 9 -0.2767171016349934 1106 10-11 -0.09161073729443814 1106 12-13 -0.06445333923699081 1106 14-15 0.3704419019991221 1106 16-17 0.03809569255756173 1106 18-19 -0.2488347654241707 1106 20-21 -0.3206725164291697 1106 22-23 0.5545674538027683 1106 24-25 0.26357617954662516 1106 26-27 0.2720251022888789 1106 28-29 -0.0113894736312119 1106 30-31 0.45519827599364504 1106 32-33 0.3844183394568219 1106 34-35 0.8088671884838448 1106 36-37 0.7073847293245734 1106 38-39 0.53449220813485 1106 40-41 0.8816489744616973 1106 42-43 1.2880236670167662 1106 44-45 1.5504180944688741 1106 46-47 1.9951034308075464 1106 48-49 2.4668469100974093 1106 50-51 2.504915341571021 1106 52-53 2.324549487736732 1106 54-55 2.207782184975933 1106 56-57 1.9433033790662506 1106 58-59 1.845268004076683 1106 60-61 1.579266203310123 1106 62-63 2.135540090169318 1106 64-65 1.8054244770630952 1106 66-67 2.165979276716129 1106 68-69 2.331932998443179 1106 70-71 1.9526516527743851 1106 72-73 1.1350217442203387 1106 74-75 1.0425080375260265 1106 76-77 1.4580694436634367 1106 78-79 1.5782950464544143 1106 80-81 1.127717239833924 1106 82-83 1.1758839316400795 1106 84-85 1.4433292786158525 1106 86-87 1.9239708224129046 1106 88-89 1.8214126430773483 1106 90-91 1.9210545331258437 1106 92-93 2.0561077269631376 1106 94-95 1.9465520224731563 1106 96-97 2.43324078852962 1106 98-99 2.0642079538053544 1106 100-101 1.836061648377072 1107 1 -0.29891624505580694 1107 2 0.004022613945103615 1107 3 0.15777304427584227 1107 4 0.14676562530097925 1107 5 0.19512652546028164 1107 6 -0.19066397217112296 1107 7 -0.10594459451299088 1107 8 -0.25762275417125124 1107 9 -0.33846126787583586 1107 10-11 0.03230341702339956 1107 12-13 -0.12836919684216497 1107 14-15 -0.38143171407991616 1107 16-17 -0.19934256327620403 1107 18-19 -0.21238441625026638 1107 20-21 -0.4480784098033723 1107 22-23 -0.8594165352961838 1107 24-25 -0.6582003491537662 1107 26-27 -0.2859718317939084 1107 28-29 -0.8292654743125283 1107 30-31 -0.5177194552276134 1107 32-33 -0.6367450057995185 1107 34-35 -1.1223625849789443 1107 36-37 -1.8483553490266402 1107 38-39 -1.6670918490956126 1107 40-41 -2.1836295130191417 1107 42-43 -1.5912984246200992 1107 44-45 -2.1913209619099447 1107 46-47 -2.673223941020076 1107 48-49 -2.947733229572151 1107 50-51 -2.6884503542218603 1107 52-53 -2.5376545660871486 1107 54-55 -2.3924988574359176 1107 56-57 -2.428183590785558 1107 58-59 -1.8241665045249214 1107 60-61 -2.2967344780048116 1107 62-63 -2.4570761591919528 1107 64-65 -2.281187684408547 1107 66-67 -2.3867374665984684 1107 68-69 -2.5899712768080505 1107 70-71 -1.896224177578194 1107 72-73 -2.237700108956293 1107 74-75 -1.8892753043237427 1107 76-77 -2.4403294662326616 1107 78-79 -2.361153088445942 1107 80-81 -2.453316655584235 1107 82-83 -2.1712034470006962 1107 84-85 -2.951109488204107 1107 86-87 -2.3863681317686627 1107 88-89 -2.4399142177640734 1107 90-91 -2.4379984391105722 1107 92-93 -1.8134630446260296 1107 94-95 -2.1772343852938327 1107 96-97 -1.9333492302065416 1107 98-99 -1.565241884382349 1107 100-101 -2.361902923246163 1108 1 0.23776637057417815 1108 2 0.04134318810778126 1108 3 0.2469277492200206 1108 4 0.1435758326375023 1108 5 0.08970387793236512 1108 6 0.07329137073158876 1108 7 -0.07771492944122116 1108 8 -0.008180967887362556 1108 9 0.07429790277807058 1108 10-11 0.19259049836310282 1108 12-13 -0.022308590781555893 1108 14-15 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2316 46-47 0.2806896892093391 2316 48-49 0.5258835045708992 2316 50-51 0.9282163124448104 2316 52-53 0.6683508320235134 2316 54-55 0.34333110207825257 2316 56-57 0.21048037682577458 2316 58-59 0.6840656142335142 2316 60-61 0.854210191360913 2316 62-63 1.020454205180517 2316 64-65 0.9252900483849764 2316 66-67 0.7301316292180005 2316 68-69 0.8388411388464689 2316 70-71 0.02199444590358368 2316 72-73 0.3324078532569317 2316 74-75 0.11031983737666096 2316 76-77 -0.08722555260243325 2316 78-79 0.38520318685769794 2316 80-81 0.7379114145912027 2316 82-83 0.8364888606916132 2316 84-85 0.5918729679362436 2316 86-87 0.5605279546011062 2316 88-89 0.6536755258256655 2316 90-91 0.24121883484354356 2316 92-93 -0.29322012964626865 2316 94-95 -0.36954207760152613 2316 96-97 -0.7729578671835995 2316 98-99 -1.3165239356643959 2316 100-101 -1.9148718841098606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 2.0 5 12.0 6 52.0 7 97.0 8 154.0 9 219.0 10 436.0 11 658.0 12 919.0 13 1207.0 14 1310.0 15 1736.0 16 2048.0 17 2556.0 18 2450.0 19 1739.0 20 1482.0 21 1629.0 22 1741.0 23 1699.0 24 2005.0 25 2133.0 26 1973.0 27 1952.0 28 2115.0 29 2689.0 30 3205.0 31 3506.0 32 4263.0 33 5259.0 34 7031.0 35 10040.0 36 12117.0 37 17629.0 38 16612.0 39 1667.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.72362871018106 23.675289611924335 16.279511088492292 20.321570589402317 2 15.179853023335768 23.75435128282264 40.2776225879926 20.788173105848983 3 16.989127164897504 30.039107825862736 34.36589453779707 18.605870471442692 4 11.612875499591732 22.209807039408656 36.41497271047316 29.762344750526452 5 10.525591989342043 40.25699428424084 34.590227341097595 14.627186385319524 6 24.30186084490094 40.086810778288715 18.78378959130173 16.827538785508615 7 21.620181357170484 38.43138940220895 21.824745369375563 18.123683871245007 8 23.933989427994327 35.07499247926425 21.811852679530706 19.17916541321071 9 24.416176028192016 17.845201770596073 22.18058360909364 35.558038592118265 10-11 21.81228243585887 29.25480252696721 27.983153551936052 20.949761485237868 12-13 23.833856203532598 28.26163565258498 28.301173234775884 19.603334909106536 14-15 21.91026687867979 28.449868924319908 27.928144741931327 21.711719455068977 16-17 18.403500025786045 32.51233432466349 28.658180192879612 20.42598545667085 18-19 17.995264064910675 28.861699521679153 33.69433020039624 19.448706213013935 20-21 18.901972581546264 27.957368172246337 33.66324294125231 19.47741630495509 22-23 18.942799432721646 29.3102410933001 33.31471055911298 18.43224891486527 24-25 18.552580686750613 30.254845502600027 33.57213459968198 17.62043921096738 26-27 18.178341047492577 29.522667594944323 34.479928144433124 17.819063213129972 28-29 16.900167604967983 28.22596587734754 36.209978941939916 18.663887575744553 30-31 17.910094976148525 28.663457819416394 34.116635867463145 19.30981133697194 32-33 18.385835231423783 29.114701963986423 35.07327345395161 17.42618935063819 34-35 18.179278450654586 30.095501706308614 34.2164304195714 17.508789423465394 36-37 17.68035830954919 29.51196657611499 33.595388741790174 19.21228637254565 38-39 17.592321975036963 28.76078808926177 34.267871265000174 19.379018670701097 40-41 17.328181611102277 28.62042437240247 35.217665994765184 18.833728021730067 42-43 18.175098095676052 28.455267082400372 34.902291119601514 18.467343702322065 44-45 17.121356678098383 29.460302725561487 34.12194975202634 19.296390844313795 46-47 18.59399711201265 28.478907378120056 32.446451901258335 20.480643608608954 48-49 18.361768877046714 28.633374876445057 32.438007649662644 20.56684859684559 50-51 17.61277928909403 28.79121918096687 32.93709629591429 20.658905234024814 52-53 17.458850831578495 29.66908762731531 33.6576561089862 19.21440543211999 54-55 17.23279900296532 29.593020757230647 32.23559241909837 20.93858782070566 56-57 18.432678671193433 30.35798702135889 30.57630323606515 20.633031071382526 58-59 17.162392721615547 30.701494269174734 32.125712861372904 20.010400147836812 60-61 18.044334043869902 31.92343395447982 30.138210823076395 19.894021178573883 62-63 17.198418496712364 32.48742962740126 30.128067385792257 20.186084490094117 64-65 17.3527010185225 32.29189049808758 30.10486054407151 20.250547939318405 66-67 17.12593687684797 31.94019459533796 29.76045176373513 21.17341676407894 68-69 17.36308978937552 31.142855914976643 29.958442384470256 21.53561191117758 70-71 17.427853106130605 30.480263016524482 30.04104261125556 22.05084126608935 72-73 17.687910954488807 29.840560402251924 29.702178864583782 22.76934977867549 74-75 17.3312132021144 29.842709183892737 29.70475740255275 23.121320211440114 76-77 17.311183186076722 29.522871374863424 30.39909839379888 22.766847045260977 78-79 17.21434098529791 29.098529791075574 30.18184163012639 23.505287593500128 80-81 16.94966242495348 28.953667771145174 30.12475879822424 23.971911005677104 82-83 17.187674588508315 28.728780781297004 29.894279943272167 24.189264686922517 84-85 17.03081352872921 28.17783316859341 29.776526709355792 25.014826593321587 86-87 17.08085494658736 28.033244097063807 29.82327775434489 25.06262320200394 88-89 17.488091389158313 27.490031253367817 29.745877788554804 25.275999568919065 90-91 17.14366981412607 27.18982584824103 30.1268321273579 25.539672210275 92-93 17.16017018350595 27.297262452189607 29.920065322961882 25.62250204134256 94-95 17.052804386971452 26.616213056912873 30.023335868906564 26.307646687209107 96-97 17.27799767071069 26.082883161644943 29.726802728104897 26.91231643953947 98-99 16.95990373458249 26.150242812325413 29.995272680390215 26.894580772701875 100-101 17.200997034681336 25.79225579096652 30.37775581245434 26.628991361897803 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1268.0 1 795.5 2 363.5 3 385.5 4 314.0 5 284.0 6 424.5 7 698.5 8 897.0 9 926.0 10 732.5 11 516.5 12 462.5 13 464.5 14 450.5 15 421.0 16 429.0 17 426.0 18 443.5 19 448.0 20 442.5 21 489.0 22 550.0 23 597.0 24 664.5 25 803.0 26 1067.5 27 1371.5 28 1475.0 29 1573.0 30 1858.5 31 2154.0 32 2524.5 33 2985.5 34 3395.0 35 3682.0 36 4038.5 37 4336.5 38 4251.5 39 4005.0 40 3805.5 41 3684.5 42 3645.5 43 3674.5 44 3758.0 45 3884.5 46 3821.0 47 3562.5 48 3380.5 49 3205.0 50 3037.5 51 2913.0 52 2637.5 53 2205.0 54 1794.0 55 1613.0 56 1513.5 57 1365.0 58 1263.5 59 1172.0 60 1052.0 61 925.0 62 802.5 63 658.0 64 535.5 65 466.0 66 419.0 67 372.5 68 329.5 69 286.5 70 281.0 71 266.0 72 236.0 73 205.0 74 171.0 75 147.0 76 116.0 77 94.0 78 78.5 79 60.0 80 36.5 81 28.0 82 25.5 83 14.5 84 9.5 85 5.5 86 3.0 87 1.5 88 1.5 89 1.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3558382397180798 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002578537968971593 18-19 4.297563281619322E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.297563281619322E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010314151875886373 36-37 0.018049765782801153 38-39 0.011173664532210238 40-41 0.0073058575787528475 42-43 0.00386780695345739 44-45 0.0017190253126477288 46-47 8.595126563238644E-4 48-49 0.0 50-51 4.297563281619322E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.297563281619322E-4 60-61 8.595126563238644E-4 62-63 0.0 64-65 0.0 66-67 8.595126563238644E-4 68-69 4.297563281619322E-4 70-71 0.002148781640809661 72-73 0.0 74-75 0.0 76-77 0.09282736688297735 78-79 0.030082942971335253 80-81 4.297563281619322E-4 82-83 0.0 84-85 0.0 86-87 0.1495552022003524 88-89 0.30727577463578154 90-91 0.27977136963341787 92-93 0.0 94-95 4.297563281619322E-4 96-97 4.297563281619322E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 116345.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.2810606386179 #Duplication Level Percentage of deduplicated Percentage of total 1 86.17503635896998 51.947225922901715 2 7.414378190321385 8.93893162576819 3 2.1487438332335245 3.885856719240191 4 1.0465679984030571 2.523529158966866 5 0.7257535574756894 2.1874597103442346 6 0.4790828984515356 1.7327775151489107 7 0.3265178087660764 1.3777987880871545 8 0.2580773947015713 1.2445743263569555 9 0.19961787435480652 1.0829859469680692 >10 1.1520803034191691 12.45347887747647 >50 0.04705278466934725 1.9407795779792858 >100 0.015684261556449083 2.0636898878335983 >500 0.004277525879031569 2.0628303751772745 >1k 0.007129209798385947 6.558081567751086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 2064 1.7740341226524559 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1575 1.3537324337100864 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1562 1.3425587691778762 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1423 1.223086509948859 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1006 0.8646697322618077 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 981 0.8431819158537109 RNA PCR Primer, Index 26 (100% over 22bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.7297262452189608 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 570 0.48992221410460274 RNA PCR Primer, Index 26 (100% over 24bp) TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 403 0.34638360049851735 RNA PCR Primer, Index 26 (100% over 29bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.3137221195582105 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 296 0.25441574627186386 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.22003524001890928 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.17620009454639218 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 189 0.16244789204521035 RNA PCR Primer, Index 26 (100% over 27bp) TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 162 0.13924105032446604 RNA PCR Primer, Index 26 (95% over 22bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.1349434870428467 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 134 0.11517469594739783 RNA PCR Primer, Index 26 (96% over 27bp) TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 119 0.10228200610253985 RNA PCR Primer, Index 26 (100% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.595126563238644E-4 0.0 18-19 0.0 0.0 0.0 8.595126563238644E-4 0.0 20-21 0.0 0.0 0.0 0.002148781640809661 0.0 22-23 0.0 0.0 0.0 0.009024882891400576 0.0 24-25 0.0 0.0 0.0 0.02191757273625854 0.0 26-27 0.0 0.0 0.0 0.0494219777386222 0.0 28-29 0.0 0.0 0.0 0.09497614852378701 0.0 30-31 0.0 0.0 0.0 0.2148781640809661 0.0 32-33 0.0 0.0 0.0 0.39537582190897763 0.0 34-35 0.0 0.0 0.0 0.6583866947440802 0.0 36-37 0.0 0.0 0.0 1.0636469122007821 0.0 38-39 0.0 0.0 0.0 1.5552881516180326 0.0 40-41 0.0 0.0 0.0 2.0765825776784563 0.0 42-43 0.0 0.0 0.0 2.5248184279513515 0.0 44-45 0.0 0.0 0.0 3.0087240534616875 0.0 46-47 0.0 0.0 0.0 3.5631097167905796 0.0 48-49 0.0 0.0 0.0 4.062486570114745 0.0 50-51 0.0 0.0 0.0 4.590227341097597 0.0 52-53 0.0 0.0 0.0 5.096050539344192 0.0 54-55 0.0 0.0 0.0 5.6637586488461045 0.0 56-57 0.0 0.0 0.0 6.2795994671021536 0.0 58-59 0.0 0.0 0.0 6.907903218874898 0.0 60-61 0.0 0.0 0.0 7.603678714169066 0.0 62-63 0.0 0.0 0.0 8.239288323520563 0.0 64-65 0.0 0.0 0.0 8.91701405303193 0.0 66-67 0.0 0.0 0.0 9.661352013408397 0.0 68-69 0.0 0.0 0.0 10.468434397696505 0.0 70-71 0.0 0.0 0.0 11.350724139412954 0.0 72-73 0.0 0.0 0.0 12.190468004641367 0.0 74-75 0.0 0.0 0.0 13.055567493231337 0.0 76-77 0.0 0.0 0.0 13.999312389874941 0.0 78-79 0.0 0.0 0.0 14.971421204177231 0.0 80-81 0.0 0.0 0.0 15.977910524732476 0.0 82-83 0.0 0.0 0.0 16.971936911771024 0.0 84-85 0.0 0.0 0.0 17.980145257638917 0.0 86-87 0.0 0.0 0.0 19.00511410030513 0.0 88-89 0.0 0.0 0.0 20.02664489234604 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACTAA 20 0.0020753697 71.19808 8 TGGACTG 35 2.7875265E-4 54.246162 5 GTCCAGA 50 2.7734603E-5 47.60867 1 TAGTCAA 45 9.6238346E-4 42.19146 9 GGTATCA 930 0.0 41.97753 1 ATTATGT 50 0.0016156242 37.972313 8 GTATCAA 1505 0.0 35.429707 1 TACTATG 55 0.0025793451 34.520287 7 CACCGCC 30 0.0038596129 31.807693 84-85 GGATAGA 30 0.0039586313 31.643595 32-33 TATCAAC 1780 0.0 29.599203 2 ATCAACG 1795 0.0 29.351858 3 TCAACGC 1815 0.0 29.028421 4 CAACGCA 1845 0.0 28.556416 5 AACGCAG 1820 0.0 28.166275 6 AGGTAGC 70 0.008390914 27.123081 7 CCCTAGG 70 0.008390914 27.123081 8 ATTCGTG 35 0.0084078945 27.123081 54-55 CGCAGAG 2000 0.0 25.631311 8 GAGTACT 1180 0.0 25.341694 12-13 >>END_MODULE