FastQCFastQC Report
Thu 26 May 2016
SRR938850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116310
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17801.530392915484481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16261.3979881351560486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14431.2406499871034304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.8331183905081249No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.8176425070931131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.3026394978935603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.276846358868541No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.236437107729344No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.19774739919181497No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.11177026910841716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG301.3007532E-463.2896963
CTGGATA250.005023629456.9607284
GACATAT352.7751163E-454.295081
ACACCTA652.581428E-643.8159376
GGATATG459.621891E-442.193139
CATTTAC500.001608469338.0065541
ATGGGGC500.001615298437.9738165
ACATATA651.32621E-436.5132832
GGTATCA8850.036.5034681
TACTTAT550.00257882734.5216527
ACCTACT550.00257882734.5216528
CAAGACT300.00394534531.66530476-77
CAGAATT600.003949770231.6448489
CACCTAC600.003949770231.6448487
TTAACAG600.003949770231.6448484
AGGATAT600.003949770231.6448488
TACACCT1051.8924275E-631.6448465
GTATCAA13300.031.4339921
CATGGGC1102.7202186E-630.2064444
TAGGATA650.005842386329.2106277
Produced by FastQC (version 0.11.5)