##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938850_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116310 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.633952368669934 31.0 31.0 34.0 27.0 34.0 2 30.655592812311927 33.0 31.0 34.0 27.0 34.0 3 30.597936548878 33.0 31.0 34.0 26.0 34.0 4 34.14455334880922 37.0 35.0 37.0 30.0 37.0 5 34.13398675952197 37.0 35.0 37.0 30.0 37.0 6 34.201074714126044 37.0 35.0 37.0 30.0 37.0 7 34.12801994669418 37.0 35.0 37.0 30.0 37.0 8 34.090903619637174 37.0 35.0 37.0 30.0 37.0 9 35.54629868454991 39.0 37.0 39.0 30.0 39.0 10-11 35.41330496088041 39.0 35.0 39.0 30.0 39.0 12-13 35.30153039291548 39.0 35.0 39.0 30.0 39.0 14-15 36.274864586020115 40.0 36.0 41.0 28.0 41.0 16-17 36.14588169546901 39.5 36.0 41.0 28.0 41.0 18-19 36.11913850915656 40.0 36.0 41.0 28.5 41.0 20-21 36.0468102484739 40.0 36.0 41.0 27.0 41.0 22-23 35.89671997248732 39.5 35.5 41.0 27.0 41.0 24-25 35.762075487920214 39.0 35.0 41.0 27.0 41.0 26-27 35.55513713352248 39.0 35.0 41.0 26.5 41.0 28-29 35.28469607084516 39.0 35.0 41.0 25.5 41.0 30-31 34.87160605278996 38.5 35.0 40.5 24.5 41.0 32-33 34.44771300833978 38.0 35.0 40.0 23.5 41.0 34-35 34.04946694179348 38.0 34.5 40.0 20.0 41.0 36-37 33.695963373742586 38.0 34.0 40.0 16.5 41.0 38-39 33.38730547674318 38.0 33.0 40.0 14.5 41.0 40-41 33.05671911271602 38.0 33.0 40.0 8.5 41.0 42-43 32.7956753503568 38.0 33.0 40.0 2.0 41.0 44-45 32.356018399105835 38.0 33.0 40.0 2.0 41.0 46-47 31.713588685409682 37.5 31.5 40.0 2.0 41.0 48-49 31.158430057604676 37.0 31.0 40.0 2.0 41.0 50-51 29.946466339953574 36.0 28.0 39.0 2.0 40.5 52-53 29.98039291548448 35.5 28.5 39.5 2.0 40.0 54-55 30.365879975926404 36.0 29.5 40.0 2.0 41.0 56-57 30.225088126558333 36.0 29.0 40.0 2.0 41.0 58-59 30.00168085289313 36.0 28.0 40.0 2.0 41.0 60-61 29.680126386381225 35.0 27.5 40.0 2.0 41.0 62-63 29.25486200670622 35.0 26.5 39.0 2.0 41.0 64-65 28.83243487232396 35.0 26.0 39.0 2.0 41.0 66-67 28.433015217952025 35.0 25.5 39.0 2.0 40.5 68-69 27.96582838964835 35.0 23.0 38.0 2.0 40.0 70-71 27.51925027942567 35.0 22.0 37.5 2.0 40.0 72-73 27.01468059496174 34.5 19.5 37.0 2.0 39.5 74-75 26.480599260596684 34.0 15.5 36.0 2.0 39.0 76-77 25.97287421545869 34.0 7.5 36.0 2.0 39.0 78-79 25.46823574929069 34.0 2.0 35.0 2.0 37.5 80-81 24.933780414409767 33.0 2.0 35.0 2.0 37.0 82-83 24.459152265497377 33.0 2.0 35.0 2.0 36.5 84-85 24.038930444501762 33.0 2.0 35.0 2.0 36.0 86-87 23.538474765712323 32.5 2.0 35.0 2.0 36.0 88-89 22.99943255094145 32.0 2.0 35.0 2.0 35.0 90-91 22.47588771386811 31.5 2.0 35.0 2.0 35.0 92-93 21.956607342446908 31.0 2.0 35.0 2.0 35.0 94-95 21.387911615510276 31.0 2.0 35.0 2.0 35.0 96-97 20.77357922792537 30.0 2.0 34.5 2.0 35.0 98-99 20.078303671223452 29.5 2.0 34.0 2.0 35.0 100-101 18.203675522311066 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7050972556589095 1101 2 0.9627689456045161 1101 3 1.033803752172659 1101 4 0.934764487564415 1101 5 1.0049899800200208 1101 6 0.9714991077664408 1101 7 1.4208159949246664 1101 8 0.8060007628409878 1101 9 1.3094552423573518 1101 10-11 1.0702399083149245 1101 12-13 0.9858911732225266 1101 14-15 0.6861407359945559 1101 16-17 0.9573620310618836 1101 18-19 1.8136331379157866 1101 20-21 2.289756854245276 1101 22-23 1.696440139027267 1101 24-25 1.583002165555719 1101 26-27 1.6640871629275082 1101 28-29 1.613811086740938 1101 30-31 0.7362099985223338 1101 32-33 0.7285483911799489 1101 34-35 0.6378827988669755 1101 36-37 0.9565629076822546 1101 38-39 0.9111336811159845 1101 40-41 0.6428020280209168 1101 42-43 0.19575062334601512 1101 44-45 0.016860893053070924 1101 46-47 0.18495145408077818 1101 48-49 0.7078360699127693 1101 50-51 0.5415386893206282 1101 52-53 0.8756588656678908 1101 54-55 0.46761249629631507 1101 56-57 0.6020274918304445 1101 58-59 0.19067304441554356 1101 60-61 0.08936564229264476 1101 62-63 0.3753011786644542 1101 64-65 -0.028417147421460953 1101 66-67 -0.027667015691200447 1101 68-69 0.11905445600459785 1101 70-71 0.5949453317861995 1101 72-73 0.5062253073631382 1101 74-75 0.5614414512631392 1101 76-77 0.908495084490081 1101 78-79 0.3453310016070006 1101 80-81 0.4626587002407554 1101 82-83 0.895712738596643 1101 84-85 0.02920624425185636 1101 86-87 0.22242543289284455 1101 88-89 -0.0011645007462313117 1101 90-91 -0.6787280037891215 1101 92-93 -1.0444174301544393 1101 94-95 -1.3108373106616256 1101 96-97 -0.6655129323270117 1101 98-99 -0.19079137382421507 1101 100-101 -0.41152408555684517 1102 1 0.9871874368641365 1102 2 0.6173833577410512 1102 3 0.8545370012244895 1102 4 0.8361551323178915 1102 5 0.8614585851611949 1102 6 1.135005218175209 1102 7 1.2748404365597281 1102 8 1.0795785125249324 1102 9 1.3148492583708347 1102 10-11 1.4633709660477479 1102 12-13 1.4500293948828684 1102 14-15 1.4679780726991467 1102 16-17 1.5252507794816026 1102 18-19 1.1274553039503417 1102 20-21 1.3271356995887231 1102 22-23 1.6843036029969198 1102 24-25 1.422614470654743 1102 26-27 1.7319759113472273 1102 28-29 1.671628195885475 1102 30-31 1.8871160246496075 1102 32-33 1.5643258880193898 1102 34-35 1.79017946974772 1102 36-37 2.0289185756131403 1102 38-39 1.8474168669566922 1102 40-41 1.501461952167567 1102 42-43 2.169665499030799 1102 44-45 2.2330429410935224 1102 46-47 2.282675853575082 1102 48-49 1.441085121745889 1102 50-51 1.2823309354226105 1102 52-53 1.0043567164896352 1102 54-55 0.7014936593810184 1102 56-57 0.6054830333390839 1102 58-59 0.8897459479132266 1102 60-61 0.9100146098442714 1102 62-63 1.4950230497137547 1102 64-65 1.0141450101849436 1102 66-67 1.3808454158300805 1102 68-69 1.981380625410413 1102 70-71 1.5709672449762557 1102 72-73 0.989284725820788 1102 74-75 1.2409189059618342 1102 76-77 1.4377829058132967 1102 78-79 0.34971363961795987 1102 80-81 1.167926294003923 1102 82-83 1.5008539101094946 1102 84-85 1.2117599737082436 1102 86-87 1.5317385386661293 1102 88-89 1.7378578338513222 1102 90-91 1.3014237029112579 1102 92-93 1.4776221821506574 1102 94-95 1.2892891115886904 1102 96-97 1.4241204431470749 1102 98-99 1.4623481120586312 1102 100-101 1.10267734722866 1103 1 -0.0713438470979817 1103 2 0.12367120124361364 1103 3 0.49069598274909865 1103 4 0.019977520145864958 1103 5 0.6245388522004731 1103 6 -0.15757357393781746 1103 7 0.13084105758130704 1103 8 0.3325671788810851 1103 9 -0.04417884285567908 1103 10-11 0.08264592335251564 1103 12-13 0.26333478224508156 1103 14-15 -0.20132793568463825 1103 16-17 -0.19226202908848933 1103 18-19 -0.13849718789874998 1103 20-21 -0.4267844991381793 1103 22-23 -0.027620011348673756 1103 24-25 -0.46536876176258346 1103 26-27 -0.6829053182754947 1103 28-29 -1.095838036066084 1103 30-31 -0.6947423824300429 1103 32-33 -0.8663137642085275 1103 34-35 -1.182417953012724 1103 36-37 -0.46987819507463513 1103 38-39 -0.5458838627436648 1103 40-41 -0.21108268375852646 1103 42-43 0.3525175406392478 1103 44-45 0.5989410935543233 1103 46-47 0.9260542109730032 1103 48-49 0.8895403305643903 1103 50-51 0.6993081128795282 1103 52-53 0.6562353067706503 1103 54-55 0.5189909424116053 1103 56-57 -0.07065421243020964 1103 58-59 0.8271392098290811 1103 60-61 1.213927045801416 1103 62-63 0.4907147124990381 1103 64-65 0.24601894280410264 1103 66-67 0.6921324830556692 1103 68-69 0.7303326514933204 1103 70-71 -0.21633286370252236 1103 72-73 0.22965889132304085 1103 74-75 -0.13918511515290177 1103 76-77 -0.36970040423172534 1103 78-79 -0.02258879789174273 1103 80-81 -0.5535067132930784 1103 82-83 -0.09175593308255614 1103 84-85 -0.7343275903346083 1103 86-87 -0.5313339656033946 1103 88-89 -1.1907634982399635 1103 90-91 -0.5961465501550336 1103 92-93 -0.21446755546772422 1103 94-95 -0.26497264900748974 1103 96-97 -0.15711694235207574 1103 98-99 -0.5693628023956414 1103 100-101 -0.5146569176370441 1104 1 0.6594486513896172 1104 2 0.8255768273779154 1104 3 0.8249367570987651 1104 4 0.6453556205693403 1104 5 1.2750228207425138 1104 6 1.2637815379799093 1104 7 1.0916698537095613 1104 8 1.2651140633335984 1104 9 1.3597836495823117 1104 10-11 1.29778506430835 1104 12-13 1.705636657623181 1104 14-15 0.9989896686710722 1104 16-17 1.5693078438697654 1104 18-19 1.3950781297056096 1104 20-21 1.470750286100781 1104 22-23 1.4905288089780058 1104 24-25 1.8821811474933199 1104 26-27 1.6424122860802228 1104 28-29 1.7278915465110458 1104 30-31 1.4000852037768539 1104 32-33 1.3119227754163987 1104 34-35 1.0778663785057319 1104 36-37 1.0928108551370954 1104 38-39 1.2999267845642564 1104 40-41 1.6765458703854534 1104 42-43 1.3165223803246633 1104 44-45 1.333363356107263 1104 46-47 1.2398775624551526 1104 48-49 1.1942892718832105 1104 50-51 0.9470395103386942 1104 52-53 1.6590332499043434 1104 54-55 2.0858390972815393 1104 56-57 1.711181843226175 1104 58-59 2.0672329787341006 1104 60-61 1.3146529986144877 1104 62-63 1.2668036417186386 1104 64-65 2.029669880493149 1104 66-67 2.1994676312710304 1104 68-69 1.9912219436893288 1104 70-71 1.6678927866302082 1104 72-73 1.6979740758360435 1104 74-75 1.098715671295981 1104 76-77 1.350202802059865 1104 78-79 1.6098219704083157 1104 80-81 0.7866324276627594 1104 82-83 0.5036766138018969 1104 84-85 0.7629500866163887 1104 86-87 0.26388684504391335 1104 88-89 0.6034742513063165 1104 90-91 0.21979416369856253 1104 92-93 0.28197830055163564 1104 94-95 0.6120690932792634 1104 96-97 -0.2162518485831697 1104 98-99 -0.4113168253841515 1104 100-101 0.08432977322804902 1105 1 -0.46503995176643187 1105 2 -0.7081753643228446 1105 3 -1.4538475795464691 1105 4 -0.9284316383435751 1105 5 -0.4101028367677131 1105 6 -0.6003330149131401 1105 7 -0.6580379195224708 1105 8 -0.8254762347377067 1105 9 -1.0296085187403108 1105 10-11 -0.45199576561567056 1105 12-13 -0.8034954288759408 1105 14-15 -0.7396865440700182 1105 16-17 -0.8729043127637865 1105 18-19 -1.1487558854901891 1105 20-21 -1.077394073922605 1105 22-23 -1.3605816527402936 1105 24-25 -1.2989994486492904 1105 26-27 -1.1534673164913798 1105 28-29 -1.3237595347279907 1105 30-31 -1.7201661112436 1105 32-33 -1.2150351439290148 1105 34-35 -1.6452487588408502 1105 36-37 -1.054252265844788 1105 38-39 -0.9549680137992738 1105 40-41 -1.4023553569669716 1105 42-43 -1.0402865033690816 1105 44-45 -0.3003303902398997 1105 46-47 0.44136791897181027 1105 48-49 -0.47315666616471574 1105 50-51 -0.14837252940414558 1105 52-53 -0.17300941520378288 1105 54-55 -0.5063584480136107 1105 56-57 0.28854888004674706 1105 58-59 0.03223882326945926 1105 60-61 -0.0032897249389911565 1105 62-63 0.2708217597782543 1105 64-65 0.60031570165037 1105 66-67 0.2489270117502862 1105 68-69 -0.5618172146975802 1105 70-71 -0.43971972067545906 1105 72-73 -0.15467057641410165 1105 74-75 -0.1078886536601864 1105 76-77 -0.2381957952516487 1105 78-79 -0.08428966021704909 1105 80-81 0.3525295638404309 1105 82-83 0.6212978879106039 1105 84-85 0.7518454694334551 1105 86-87 0.4092696621099563 1105 88-89 0.4343480093425498 1105 90-91 -0.6105681974937198 1105 92-93 -0.4629807877008467 1105 94-95 -0.40078888451150263 1105 96-97 0.31136358099018224 1105 98-99 -1.0848995255594858 1105 100-101 -1.1463102033938064 1106 1 0.35809932605228667 1106 2 -0.022324222097349633 1106 3 -0.2233391049702007 1106 4 0.26147256209857517 1106 5 0.5569568537260281 1106 6 0.11145769989894916 1106 7 0.35456340693294663 1106 8 0.20724299886582997 1106 9 -0.31000645536521887 1106 10-11 0.14332479444328783 1106 12-13 -0.07228688060770594 1106 14-15 0.18448442129476206 1106 16-17 -0.005992006205197242 1106 18-19 -0.365041810324378 1106 20-21 -0.406723477017664 1106 22-23 -0.05759712805347306 1106 24-25 -0.5900827205726458 1106 26-27 -0.603614700426526 1106 28-29 -0.2586526813750112 1106 30-31 -0.12735107808222068 1106 32-33 -0.6459857703107375 1106 34-35 -1.042448464072983 1106 36-37 -0.9295654567069747 1106 38-39 -0.46712718844923984 1106 40-41 -0.7764526303641688 1106 42-43 -0.6069408880411515 1106 44-45 -0.2415242001146325 1106 46-47 -0.15045227262730165 1106 48-49 -0.5437167251182906 1106 50-51 -0.9127056170975898 1106 52-53 -2.6162666001706185 1106 54-55 -2.455989988175734 1106 56-57 -2.3350325495774236 1106 58-59 -1.9778569762883897 1106 60-61 -2.604837256258076 1106 62-63 -3.209170871024991 1106 64-65 -3.0652701494918553 1106 66-67 -2.8237332682791916 1106 68-69 -3.129392748964346 1106 70-71 -3.042114709621668 1106 72-73 -2.7428430156182273 1106 74-75 -2.951809066335205 1106 76-77 -3.037053569754228 1106 78-79 -2.649285975618671 1106 80-81 -2.5058713204631786 1106 82-83 -2.2479725616103963 1106 84-85 -2.8943962402201926 1106 86-87 -2.784613904581274 1106 88-89 -3.485843589773147 1106 90-91 -3.517237320559307 1106 92-93 -2.829303558518827 1106 94-95 -2.970464639854171 1106 96-97 -3.0543328081034105 1106 98-99 -3.3135657009463664 1106 100-101 -2.6212549344181255 1107 1 -0.007118065252061712 1107 2 0.18637143007656576 1107 3 0.646226112421104 1107 4 0.6440812577507486 1107 5 0.44391337546515786 1107 6 0.32015335207285744 1107 7 0.02412862432424845 1107 8 0.042025607561484435 1107 9 -0.5273977597130397 1107 10-11 -0.13928390120889134 1107 12-13 0.3885826846096876 1107 14-15 0.010571377816496863 1107 16-17 0.6635732111861117 1107 18-19 0.35234949402344284 1107 20-21 0.5324069577649411 1107 22-23 0.30327243716391195 1107 24-25 -0.15095228579004072 1107 26-27 0.11812443000825823 1107 28-29 -0.31517442050544275 1107 30-31 -0.16648151286482715 1107 32-33 0.19749249055882956 1107 34-35 0.7314645794052765 1107 36-37 -0.003478500185238431 1107 38-39 -0.5279967536666277 1107 40-41 0.12354736963582269 1107 42-43 -0.1808539315194153 1107 44-45 0.49325840858102055 1107 46-47 0.5278085969379127 1107 48-49 0.32150066618605777 1107 50-51 0.5568596002937163 1107 52-53 1.1098203563511184 1107 54-55 0.7744447944329629 1107 56-57 0.19975005911822663 1107 58-59 -0.034378715418821315 1107 60-61 0.3647279611332266 1107 62-63 0.2125682594097924 1107 64-65 -0.017444062535336258 1107 66-67 -0.10199413784441091 1107 68-69 0.08799855538348211 1107 70-71 -0.08994079657818688 1107 72-73 -0.24719084170518357 1107 74-75 -0.19528732720477038 1107 76-77 0.345555125897949 1107 78-79 -0.27102510605345387 1107 80-81 0.15065041866725792 1107 82-83 0.3215926557809077 1107 84-85 -0.07700493587236679 1107 86-87 0.11973392150568074 1107 88-89 -0.3814957636861891 1107 90-91 -0.23897645099409104 1107 92-93 0.383739919742041 1107 94-95 0.19910057753713417 1107 96-97 -0.31085454723384487 1107 98-99 -0.1526961357289771 1107 100-101 0.08744071775578632 1108 1 0.8286397567715049 1108 2 1.0444778908648615 1108 3 0.48881042774008066 1108 4 0.8760194942399764 1108 5 0.7871884668914078 1108 6 0.519340674299599 1108 7 0.3916215133937371 1108 8 0.5651462902108122 1108 9 0.5725616063982955 1108 10-11 0.6214637623425148 1108 12-13 0.6948809373524725 1108 14-15 0.9194295655450659 1108 16-17 0.9110335931446585 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0.10987846280546876 2311 62-63 0.2692572226204959 2311 64-65 0.4785425595382442 2311 66-67 0.8906480026238199 2311 68-69 0.9157577527078935 2311 70-71 0.27260100944187826 2311 72-73 0.6573242084620396 2311 74-75 0.37444511426680194 2311 76-77 0.7693009452959387 2311 78-79 0.8649280712040728 2311 80-81 0.22456346214551814 2311 82-83 0.5125625554464612 2311 84-85 0.38085459590020676 2311 86-87 0.053011513478924854 2311 88-89 0.19041059082886136 2311 90-91 0.28226100719989233 2311 92-93 0.514007478270468 2311 94-95 0.4342176343933204 2311 96-97 -0.2292491960632752 2311 98-99 -1.1103884434212823 2311 100-101 -1.219582693615454 2312 1 -0.26553160657300623 2312 2 -0.5741994295317987 2312 3 -0.39433147138241154 2312 4 -0.7119094491858604 2312 5 -0.8191466975517727 2312 6 -0.7838625788929576 2312 7 -0.7059742631633057 2312 8 -0.667499782415284 2312 9 -0.4616825257269781 2312 10-11 -1.034703748537133 2312 12-13 -0.6652175145273844 2312 14-15 -1.1229832189309263 2312 16-17 -0.9811330616316809 2312 18-19 -1.229296705777415 2312 20-21 -1.5510646652022047 2312 22-23 -0.7383472110272606 2312 24-25 -1.082283185225819 2312 26-27 -0.8812781587736893 2312 28-29 -1.5179955290351472 2312 30-31 -1.6546660465521796 2312 32-33 -1.490426527031616 2312 34-35 -1.9943236715074306 2312 36-37 -1.6965087026412675 2312 38-39 -1.7865762425481435 2312 40-41 -2.4080158563847505 2312 42-43 -1.5042857270720162 2312 44-45 -1.8064760753220632 2312 46-47 -1.9103593419933844 2312 48-49 -1.778532523326053 2312 50-51 -1.8311257963209542 2312 52-53 -1.6471328464368007 2312 54-55 -1.7679382125368335 2312 56-57 -2.3749263431386574 2312 58-59 -1.9497122700155707 2312 60-61 -1.7531279963841975 2312 62-63 -1.6220815912374 2312 64-65 -0.8194022437500692 2312 66-67 -0.6973144166363134 2312 68-69 -0.3632792408329131 2312 70-71 -0.5209398008928261 2312 72-73 -0.8599688038691404 2312 74-75 -0.5375043866139926 2312 76-77 -0.5924136759606675 2312 78-79 -0.32177187007602015 2312 80-81 -0.23192508747280272 2312 82-83 -1.0490346259980505 2312 84-85 -0.23000858671870006 2312 86-87 -0.15852694805953504 2312 88-89 -0.6936763146320928 2312 90-91 -0.5101347649668746 2312 92-93 -0.9969437842075131 2312 94-95 -0.2744141917957599 2312 96-97 -0.333124709862453 2312 98-99 0.02669317724812359 2312 100-101 0.6502352746757971 2313 1 -0.013503934817279628 2313 2 -0.6033024829669138 2313 3 -0.4514641049701993 2313 4 0.3465948447072691 2313 5 0.17292152763906898 2313 6 -0.04710208270974903 2313 7 -0.1254637669800971 2313 8 -0.7437760228732984 2313 9 -0.2002917814521723 2313 10-11 -0.2322526512088885 2313 12-13 -0.09878144582509663 2313 14-15 0.14756186694693696 2313 16-17 0.2405093524904558 2313 18-19 0.4807462331538801 2313 20-21 0.29820179472146435 2313 22-23 0.25157406759869616 2313 24-25 0.3839322250795263 2313 26-27 -0.056671765643919514 2313 28-29 0.04766525340760097 2313 30-31 0.016195117569957063 2313 32-33 0.9323430340370891 2313 34-35 0.4453232750574543 2313 36-37 0.7090554672060634 2313 38-39 0.4955086811159788 2313 40-41 0.10731095659235024 2313 42-43 0.22573574239363126 2313 44-45 -0.22253642837549847 2313 46-47 0.5262460969379141 2313 48-49 0.9946664270556234 2313 50-51 1.208354165511107 2313 52-53 1.5099222585250338 2313 54-55 1.2633714248677457 2313 56-57 0.7016182656399685 2313 58-59 0.647109056320307 2313 60-61 0.3021260589593133 2313 62-63 0.8398101072358806 2313 64-65 0.8540233287690135 2313 66-67 0.9535085795468952 2313 68-69 0.13925534886174162 2313 70-71 0.6156298555957243 2313 72-73 0.07553334307742432 2313 74-75 0.19031049888218732 2313 76-77 0.03702931068055548 2313 78-79 0.11817326351176405 2313 80-81 0.7605009621455174 2313 82-83 0.27145678621569047 2313 84-85 0.26012142282328554 2313 86-87 0.44694180194046496 2313 88-89 0.9140884754442453 2313 90-91 1.322164853353737 2313 92-93 0.766892093655084 2313 94-95 0.845635903624089 2313 96-97 1.3700897462444175 2313 98-99 0.1324601142710229 2313 100-101 0.5429774025383942 2314 1 -0.14750014693849067 2314 2 -0.14307521023964043 2314 3 -0.8884906201217149 2314 4 -0.12475932195939521 2314 5 0.2616999367299755 2314 6 0.3416289778963133 2314 7 0.41951729362596524 2314 8 -0.0039559471157204484 2314 9 0.34019117309328095 2314 10-11 0.13268579576081407 2314 12-13 0.16684355417490337 2314 14-15 0.26557796543178114 2314 16-17 -0.1386336399337864 2314 18-19 -0.05097725169460432 2314 20-21 0.10371789320630853 2314 22-23 -0.47498843240130384 2314 24-25 -0.23550432795077114 2314 26-27 -0.17433275049240393 2314 28-29 -0.3270980041681568 2314 30-31 -0.24777268546034037 2314 32-33 -0.25586719323563756 2314 34-35 0.45952782051200103 2314 36-37 0.09790489902425037 2314 38-39 -0.23129056130826342 2314 40-41 -0.26271745249855627 2314 42-43 -0.6291411515457597 2314 44-45 -0.8159313147391352 2314 46-47 -0.5843126151832969 2314 48-49 -0.8630513759746812 2314 50-51 -0.8375738647919242 2314 52-53 -1.026180961171935 2314 54-55 -1.2969978933140744 2314 56-57 -1.9362841964812425 2314 58-59 -1.190604011861513 2314 60-61 -0.8757858728588666 2314 62-63 -0.011278907915635017 2314 64-65 -0.3287418227461387 2314 66-67 -0.662731950756136 2314 68-69 -0.6873308253806805 2314 70-71 -0.41230575046488127 2314 72-73 0.08512141125924444 2314 74-75 -0.23109101626932826 2314 76-77 -0.1266780756830812 2314 78-79 0.15344788472388515 2314 80-81 0.18213921972127523 2314 82-83 0.5077257256096281 2314 84-85 0.49035343039904333 2314 86-87 0.7505639610313715 2314 88-89 0.35608658150485084 2314 90-91 -0.1465851466462631 2314 92-93 -0.12515336089036921 2314 94-95 0.20193325210893676 2314 96-97 -0.08694150375558252 2314 98-99 -0.5276961357289771 2314 100-101 0.39702569799294096 2315 1 -1.149664649102995 2315 2 -0.5872310543954846 2315 3 -0.36619624782734306 2315 4 -0.6866640838641587 2315 5 -0.4045338295037908 2315 6 -0.804691368424038 2315 7 -1.141088766980097 2315 8 -0.8082849514447261 2315 9 -0.6357828528807445 2315 10-11 -0.38928390120889134 2315 12-13 -0.43315644582509805 2315 14-15 -0.6186211687673477 2315 16-17 -0.7140665403666873 2315 18-19 -1.201843052560406 2315 20-21 -1.2126240981356773 2315 22-23 -1.5785598609727316 2315 24-25 -1.4717597392061847 2315 26-27 -0.857341408501064 2315 28-29 -1.2016651037352517 2315 30-31 -0.42688523957289704 2315 32-33 -0.48573732310576645 2315 34-35 -1.0644981535139735 2315 36-37 -0.7839132827939395 2315 38-39 -1.0995806045983016 2315 40-41 -1.1214836862647886 2315 42-43 -0.46734461474922995 2315 44-45 -0.6938533926612145 2315 46-47 -0.9793342602049435 2315 48-49 0.022121784198478878 2315 50-51 -0.3858422630603222 2315 52-53 0.2185160085250324 2315 54-55 -0.010958932275112687 2315 56-57 0.4794084442113977 2315 58-59 0.6704349491774515 2315 60-61 0.6631751661021674 2315 62-63 1.1181583215215944 2315 64-65 1.1942019001975837 2315 66-67 1.1961425081183243 2315 68-69 1.576197313147457 2315 70-71 1.2687548555957235 2315 72-73 1.6312253073631382 2315 74-75 2.0519176417393297 2315 76-77 1.5989712749662708 2315 78-79 1.6429500492260516 2315 80-81 1.656706319288375 2315 82-83 2.1576175005014058 2315 84-85 1.9220633871090023 2315 86-87 2.0521873376547504 2315 88-89 1.7143116897299606 2315 90-91 2.364129139068023 2315 92-93 2.5365349507979396 2315 94-95 1.9284484036240883 2315 96-97 2.634487067672989 2315 98-99 2.7687324356995937 2315 100-101 2.6122854382526803 2316 1 -1.2010039348172796 2316 2 -1.0486149829669138 2316 3 -1.2952141049701993 2316 4 -1.715905155292731 2316 5 -2.186453472360931 2316 6 -2.070539582709749 2316 7 -1.805151266980097 2316 8 -1.9469010228732984 2316 9 -1.2862292814521723 2316 10-11 -1.2283464012088885 2316 12-13 -2.0167501958250966 2316 14-15 -2.266500633053063 2316 16-17 -2.302459397509544 2316 18-19 -2.28487876684612 2316 20-21 -2.0728919552785356 2316 22-23 -2.146863432401304 2316 24-25 -2.4207552749204737 2316 26-27 -2.3183905156439195 2316 28-29 -2.674990996592399 2316 30-31 -2.218179882430043 2316 32-33 -1.723906965962911 2316 34-35 -2.4531142249425457 2316 36-37 -2.1893820327939366 2316 38-39 -1.9654288188840212 2316 40-41 -1.7130015434076498 2316 42-43 -2.3992642576063687 2316 44-45 -2.4256614283754985 2316 46-47 -2.251097653062086 2316 48-49 -2.2983023229443766 2316 50-51 -2.049458334488893 2316 52-53 -2.599452741474966 2316 54-55 -2.7405348251322543 2316 56-57 -2.2163504843600315 2316 58-59 -2.614609693679693 2316 60-61 -2.1002176910406867 2316 62-63 -2.2305023927641194 2316 64-65 -2.2553516712309865 2316 66-67 -2.468366420453105 2316 68-69 -2.1576196511382584 2316 70-71 -1.9273388944042757 2316 72-73 -2.3150916569225757 2316 74-75 -2.0010957511178127 2316 76-77 -1.8067206893194445 2316 78-79 -1.221670486488236 2316 80-81 -1.1340302878544826 2316 82-83 -0.9902619637843095 2316 84-85 -0.6656598271767145 2316 86-87 -0.642901948059535 2316 88-89 -0.3437240245557547 2316 90-91 -0.9004913966462631 2316 92-93 -0.45967040634491596 2316 94-95 -0.834051596375911 2316 96-97 -2.0400665037555825 2316 98-99 -1.4456648857289771 2316 100-101 -1.4726475974616058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3230.0 3 618.0 4 494.0 5 414.0 6 447.0 7 470.0 8 531.0 9 566.0 10 613.0 11 829.0 12 1105.0 13 1405.0 14 1461.0 15 1771.0 16 2136.0 17 2490.0 18 2257.0 19 1336.0 20 1284.0 21 1197.0 22 1378.0 23 1475.0 24 1617.0 25 1753.0 26 1908.0 27 2097.0 28 2363.0 29 2813.0 30 3147.0 31 3590.0 32 4191.0 33 4991.0 34 6473.0 35 9671.0 36 11070.0 37 15445.0 38 15282.0 39 2392.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29638313277937 21.91669827259249 15.932834534358514 18.854084060269628 2 15.919052744886974 23.555651237890203 39.25252960172228 21.272766415500538 3 18.122690190470447 29.574176652509887 32.47215306553183 19.83098009148784 4 11.929694631508243 20.264893818570123 38.40443671311207 29.400974836809564 5 11.408233438757659 39.16010996302967 34.20229405630866 15.229362541904015 6 25.546558704453442 38.598501162890855 19.303126884313894 16.551813248341805 7 22.94519662509803 37.767704015237044 22.854705126989735 16.432394232675186 8 24.33764845810422 33.67090134969748 22.148483960491614 19.842966231706686 9 26.330064318371633 17.41417759447568 23.4111984570479 32.84455963010478 10-11 22.729896445371057 28.751916880610644 27.990333752606094 20.5278529214122 12-13 24.659245601640446 28.460100287767304 28.46225423465959 18.41839987593266 14-15 22.54757157805442 28.30008310762222 29.259480943379163 19.892864370944196 16-17 18.245343288343243 31.51346900770335 30.02021752156464 20.22097018238877 18-19 17.863959705807588 28.737187248215157 35.10230812648378 18.296544919493478 20-21 19.471401312020536 27.885389880298582 34.9597908348086 17.683417972872274 22-23 19.43465333735511 28.13849269616926 34.442625070021975 17.984228896453654 24-25 18.435010405401332 28.864971067383056 34.805313433093055 17.894705094122564 26-27 18.32297466907626 28.394960556564346 35.19070437479243 18.091360399566963 28-29 17.799241117626767 28.280010348396 35.968006209037604 17.952742324939635 30-31 18.37552860843963 28.826133625316302 34.986227082857354 17.812110683386713 32-33 17.757975390101773 29.250203503271127 34.90996326173751 18.08185784488959 34-35 18.1513467810122 28.661156454368196 34.87567511604231 18.311821648577293 36-37 18.633556456102646 28.798805408461405 34.11749981658186 18.45013831885409 38-39 18.336792237098145 28.93052996733817 34.283704895823 18.44897289974069 40-41 17.885284717999145 28.728419182182428 34.907983662625455 18.478312437192972 42-43 17.98515833980499 29.05298127534731 34.091811200276126 18.870049184571577 44-45 17.800340015706308 29.105430758476658 33.88377331135601 19.210455914461022 46-47 18.306486064303993 29.278985773033057 32.87779997669833 19.536728185964623 48-49 18.39962019033643 29.296273117676257 32.5000539502363 19.804052741751008 50-51 18.29120397743702 29.634408973401754 32.169938758863566 19.904448290297662 52-53 18.143603775457798 29.857104014984444 32.099159714639605 19.90013249491815 54-55 18.39931643406437 29.449004427642993 31.803077772886944 20.348601365405695 56-57 18.31132356303739 29.805298738950174 31.48094619722077 20.402431500791664 58-59 18.188405484556892 29.07729533191576 31.818711155009037 20.915588028518314 60-61 18.495920936890943 28.986788319908936 31.832215112368274 20.685075630831854 62-63 18.86960986955377 28.79008211682769 31.68682549203212 20.653482521586415 64-65 18.75075536525147 28.756539304891316 31.834976432604158 20.65772889725306 66-67 18.519062089923338 28.827094412597553 31.58410456523241 21.0697389322467 68-69 18.656110784957004 28.943347031805782 31.261439375625926 21.139102807611284 70-71 18.75946973784744 28.880811882983178 31.199305876309573 21.160412502859806 72-73 18.947232113952737 28.406172439840294 31.005071760008633 21.64152368619834 74-75 18.784287150977843 28.700854184381246 30.705748803321782 21.80910986131913 76-77 18.837952953665706 28.455221400860488 31.006908932416742 21.699916713057064 78-79 19.059862982967783 28.206698993942954 31.029439679718372 21.703998343370895 80-81 19.2063752308303 28.292891289715755 30.497644236577326 22.00308924287662 82-83 19.311811995391366 28.2149314530571 30.400148442860285 22.073108108691244 84-85 19.592976338944727 27.34463666924149 30.468085840910362 22.59430115090342 86-87 19.91628729853934 27.204470430861505 30.581026559364823 22.29821571123433 88-89 20.478506479775245 26.679066636170223 30.45731720474191 22.38510967931262 90-91 20.521331514701565 26.5336072791312 30.541791122084287 22.403270084082944 92-93 20.960446124894254 26.40924707791647 30.317587748830306 22.31271904835897 94-95 21.0279809028827 25.971906862702777 30.14486872890209 22.855243505512437 96-97 21.56806036380589 25.388281202786818 29.825780663207603 23.21787777019969 98-99 21.47367966676019 25.472989014309455 29.97086309973453 23.08246821919582 100-101 22.59406461858464 24.57914201502617 30.288397222617796 22.53839614377139 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1447.0 1 912.5 2 415.5 3 417.0 4 337.0 5 314.5 6 455.5 7 737.5 8 997.5 9 1019.0 10 756.0 11 541.0 12 510.0 13 480.5 14 445.5 15 434.0 16 444.5 17 453.0 18 447.0 19 461.0 20 495.5 21 563.0 22 595.5 23 652.5 24 748.0 25 844.5 26 1110.5 27 1385.5 28 1502.5 29 1639.0 30 1919.0 31 2194.5 32 2554.5 33 2951.0 34 3386.5 35 3787.0 36 3998.0 37 4176.0 38 4064.5 39 3838.5 40 3650.0 41 3487.0 42 3334.0 43 3257.0 44 3330.0 45 3357.0 46 3256.5 47 3036.5 48 2916.5 49 2846.0 50 2759.0 51 2646.0 52 2409.5 53 2255.5 54 2101.5 55 1908.5 56 1764.0 57 1595.5 58 1509.5 59 1393.0 60 1239.0 61 1140.5 62 1007.5 63 832.0 64 687.5 65 592.5 66 527.0 67 490.5 68 447.5 69 411.5 70 382.5 71 329.0 72 287.5 73 265.5 74 238.0 75 195.0 76 162.0 77 140.0 78 109.0 79 74.0 80 57.5 81 47.0 82 35.0 83 26.5 84 19.0 85 12.0 86 7.0 87 6.0 88 4.0 89 3.0 90 2.5 91 2.0 92 2.5 93 2.0 94 1.5 95 1.5 96 0.5 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2562118476485255 2 0.15905769065428596 3 0.19688762789098097 4 0.1616370045567879 5 0.23299802252600807 6 0.1891496861834752 7 0.23815665033101197 8 0.24417504943684978 9 0.1453013498409423 10-11 0.20290602699681884 12-13 0.20978419740349066 14-15 0.16808528931304273 16-17 0.27641647321812396 18-19 0.22654973776975326 20-21 0.19731751354139798 22-23 0.236437107729344 24-25 0.22740950907058724 26-27 0.33058206517066463 28-29 0.30091995529189236 30-31 0.27641647321812396 32-33 0.18786002923222425 34-35 0.3473476055369272 36-37 0.3907660562290431 38-39 0.3658326885048577 40-41 0.32628320866649474 42-43 0.3611039463502708 44-45 0.37228097326111254 46-47 0.3770097154156994 48-49 0.3976442266357149 50-51 0.39248559883071105 52-53 0.39248559883071105 54-55 0.3843177714727882 56-57 0.3563752041956839 58-59 0.33058206517066463 60-61 0.3026394978935603 62-63 0.3770097154156994 64-65 0.4058120539936377 66-67 0.4109706817986416 68-69 0.4144097670019775 70-71 0.4131201100507265 72-73 0.40624193964405464 74-75 0.4028028544407188 76-77 0.3830281145215373 78-79 0.354225775943599 80-81 0.3645430315536068 82-83 0.37872925801736734 84-85 0.3821683432207033 86-87 0.3761499441148654 88-89 0.3864671997248732 90-91 0.4058120539936377 92-93 0.40237296879030177 94-95 0.41354999570114354 96-97 0.4118304530994756 98-99 0.41054079614822453 100-101 0.3830281145215373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 116310.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.77336428510017 #Duplication Level Percentage of deduplicated Percentage of total 1 87.86290375289212 57.790387756856674 2 6.554162690683782 8.621786604763134 3 1.9006287499509809 3.7503224142378127 4 0.9699219618044208 2.551801220875247 5 0.5829989150468621 1.9172900008597713 6 0.3934589090338688 1.5527469693061644 7 0.29149945752343104 1.34210300060184 8 0.20914759284192364 1.1005072650674919 9 0.16993241918406296 1.0059324219757546 >10 1.0104443079175436 11.637004556787895 >50 0.0405223461131227 1.6911701487404351 >100 0.007843034731572137 1.2191557045825812 >500 0.0026143449105240453 1.6507608976012382 >1k 0.003921517365786068 4.16903103774396 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1780 1.530392915484481 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1626 1.3979881351560486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1443 1.2406499871034304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 969 0.8331183905081249 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 951 0.8176425070931131 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 0.3026394978935603 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 322 0.276846358868541 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 275 0.236437107729344 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 230 0.19774739919181497 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.11177026910841716 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0012896569512509672 0.0 22-23 0.0 0.0 0.0 0.005158627805003869 0.0 24-25 0.0 0.0 0.0 0.01461611211417763 0.0 26-27 0.0 0.0 0.0 0.03654028028544407 0.0 28-29 0.0 0.0 0.0 0.0907058722379847 0.0 30-31 0.0 0.0 0.0 0.2003267130943169 0.0 32-33 0.0 0.0 0.0 0.3628234889519388 0.0 34-35 0.0 0.0 0.0 0.6164560226979623 0.0 36-37 0.0 0.0 0.0 0.982718596853237 0.0 38-39 0.0 0.0 0.0 1.4383973862952453 0.0 40-41 0.0 0.0 0.0 1.920729086063107 0.0 42-43 0.0 0.0 0.0 2.332989424813 0.0 44-45 0.0 0.0 0.0 2.7899578712062594 0.0 46-47 0.0 0.0 0.0 3.2963631673974723 0.0 48-49 0.0 0.0 0.0 3.7958902931820138 0.0 50-51 0.0 0.0 0.0 4.340555412260339 0.0 52-53 0.0 0.0 0.0 4.796664087352765 0.0 54-55 0.0 0.0 0.0 5.337460235577336 0.0 56-57 0.0 0.0 0.0 5.980999054251569 0.0 58-59 0.0 0.0 0.0 6.632705700283725 0.0 60-61 0.0 0.0 0.0 7.309775599690482 0.0 62-63 0.0 0.0 0.0 7.955033960966383 0.0 64-65 0.0 0.0 0.0 8.589115295331442 0.0 66-67 0.0 0.0 0.0 9.313902501934486 0.0 68-69 0.0 0.0 0.0 10.129395580775514 0.0 70-71 0.0 0.0 0.0 11.068265841286218 0.0 72-73 0.0 0.0 0.0 11.917719886510188 0.0 74-75 0.0 0.0 0.0 12.793396956409595 0.0 76-77 0.0 0.0 0.0 13.698736136187774 0.0 78-79 0.0 0.0 0.0 14.662539764422664 0.0 80-81 0.0 0.0 0.0 15.6224744218038 0.0 82-83 0.0 0.0 0.0 16.62668730117789 0.0 84-85 0.0 0.0 0.0 17.618003611039462 0.0 86-87 0.0 0.0 0.0 18.601152093543117 0.0 88-89 0.0 0.0 0.0 19.625569598486805 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 30 1.3007532E-4 63.289696 3 CTGGATA 25 0.0050236294 56.960728 4 GACATAT 35 2.7751163E-4 54.29508 1 ACACCTA 65 2.581428E-6 43.815937 6 GGATATG 45 9.621891E-4 42.19313 9 CATTTAC 50 0.0016084693 38.006554 1 ATGGGGC 50 0.0016152984 37.973816 5 ACATATA 65 1.32621E-4 36.513283 2 GGTATCA 885 0.0 36.503468 1 TACTTAT 55 0.002578827 34.521652 7 ACCTACT 55 0.002578827 34.521652 8 CAAGACT 30 0.003945345 31.665304 76-77 CAGAATT 60 0.0039497702 31.644848 9 CACCTAC 60 0.0039497702 31.644848 7 TTAACAG 60 0.0039497702 31.644848 4 AGGATAT 60 0.0039497702 31.644848 8 TACACCT 105 1.8924275E-6 31.644846 5 GTATCAA 1330 0.0 31.433992 1 CATGGGC 110 2.7202186E-6 30.206444 4 TAGGATA 65 0.0058423863 29.210627 7 >>END_MODULE