FastQCFastQC Report
Thu 26 May 2016
SRR938850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116310
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT20211.7375977989854698No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16901.4530134984094232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15661.34640185710601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13881.1933625655575617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.8597713008339782No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT9360.8047459375806035RNA PCR Primer, Index 26 (100% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.7737941707505804No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT5290.4548190181411745RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG4210.3619637176511048RNA PCR Primer, Index 26 (100% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.275986587567707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.23385779382684205No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.21322328260682658No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.17969220187430143No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1970.1693749462642937RNA PCR Primer, Index 26 (100% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1800.15475883415011607RNA PCR Primer, Index 26 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.12552660992176082No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG1280.1100507265067492RNA PCR Primer, Index 26 (96% over 27bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.10059324219757544No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCAG156.625822E-494.917028
TGAGATG500.001616774737.9668124
GGTATCA10700.034.655521
TATGAGA702.052553E-433.8989372
CATAGAC600.003953367531.6390062
CCAGTTG300.003961440631.63900612-13
GACTTTA650.00584769729.2052387
TTATAGG650.00584769729.2052383
AGACGAG650.00584769729.2052385
GTATCAA15850.029.0940461
TAGGACT856.4049894E-427.916774
ACATAGA700.00832658727.1658691
GACGAGT700.00839684727.119156
GGCTAGT350.00841384127.1191512-13
ATGGGAC908.9446E-426.3658397
ATCAACG18650.024.6835163
AACGCAG18650.024.6835166
GCTTATA1351.32055375E-524.6505091
TCAACGC18700.024.6175164
CAACGCA18750.024.5518725
Produced by FastQC (version 0.11.5)